| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,183,656 | T→A | intergenic (‑5/+60) | yegX ← / ← thiD | putative family 25 glycosyl hydrolase/hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,183,656 | 0 | T | A | 98.4% | 215.6 / ‑3.7 | 62 | intergenic (‑5/+60) | yegX/thiD | putative family 25 glycosyl hydrolase/hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (26/35); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
CGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCAATA > NC_000913/2183559‑2183752 | cGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATc < 2:669995/100‑1 (MQ=255) cTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTatcatc < 1:575403/100‑1 (MQ=255) tGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATcc > 2:690334/1‑100 (MQ=255) gACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATccc < 1:101987/100‑1 (MQ=255) gACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATccc > 1:1244520/1‑100 (MQ=255) aCGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCt > 1:35861/1‑100 (MQ=255) cgcgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTg < 2:934008/100‑1 (MQ=255) cgcgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTGTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTg > 1:385955/1‑100 (MQ=255) gcgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGa > 2:195428/1‑100 (MQ=255) gATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATg < 2:325185/100‑1 (MQ=255) gATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATg < 2:749212/100‑1 (MQ=255) aaTTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAg < 1:228318/100‑1 (MQ=255) gCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGcc > 1:328771/1‑100 (MQ=255) cAACGATGGAAATGAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCa > 1:875917/1‑100 (MQ=255) cGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAg < 1:839764/100‑1 (MQ=255) cGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAg < 1:1227321/100‑1 (MQ=255) cGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAg < 2:905677/100‑1 (MQ=255) tGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCt < 1:724394/100‑1 (MQ=255) ggAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTg < 2:911568/100‑1 (MQ=255) ggAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTg < 2:707804/100‑1 (MQ=255) gAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGa > 1:295027/1‑100 (MQ=255) aaaTTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGa > 1:1261990/1‑99 (MQ=255) aaaTTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGAc < 2:411649/100‑1 (MQ=255) aaaTTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGAc < 1:164371/100‑1 (MQ=255) aTTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACaa > 2:486977/1‑100 (MQ=255) tAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATa < 1:1000960/100‑1 (MQ=255) cccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAcc > 1:1283936/1‑100 (MQ=255) cccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAcc < 1:852325/100‑1 (MQ=255) cccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAcc < 2:1264304/100‑1 (MQ=255) ccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCa > 2:569558/1‑100 (MQ=255) ccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCa > 1:876099/1‑100 (MQ=255) cAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCaa < 2:348960/100‑1 (MQ=255) cAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCaa < 1:1277433/100‑1 (MQ=255) cAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCaa < 1:707386/100‑1 (MQ=255) agCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAagca > 1:378165/1‑100 (MQ=255) agCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAagca > 2:557166/1‑100 (MQ=255) cATAATAGAGAGGTGAGTTTTTTTTTTTTTTTTATTCTTAGATGCATTGGTATCATTCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCta > 2:523960/1‑100 (MQ=255) cATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCta > 1:733236/1‑100 (MQ=255) ataataGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCtat < 2:353475/100‑1 (MQ=255) taataGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCtata > 1:871635/1‑100 (MQ=255) agagagGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTAc < 1:1180713/100‑1 (MQ=255) gagGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACgg > 2:382236/1‑99 (MQ=255) ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc < 2:1230367/100‑1 (MQ=255) tGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTc < 1:557170/100‑1 (MQ=255) tttttCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCaccacc < 2:1185958/100‑1 (MQ=255) cTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTgg < 2:875911/100‑1 (MQ=255) cTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTgg < 2:884739/100‑1 (MQ=255) tGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGa < 1:705955/100‑1 (MQ=255) ggTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGaa > 1:862324/1‑100 (MQ=255) ttCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAgtggt < 1:195430/100‑1 (MQ=255) ttCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAgtggt < 1:1151989/100‑1 (MQ=255) tCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAgtggtg < 1:1160375/100‑1 (MQ=255) cTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGa > 2:270674/1‑100 (MQ=255) tAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAAc > 2:522570/1‑100 (MQ=255) gCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg > 2:1293450/1‑100 (MQ=255) gCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg > 1:298574/1‑100 (MQ=255) gCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGAc > 2:465117/1‑100 (MQ=255) gCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGAc < 2:1283925/100‑1 (MQ=255) gCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGAc < 1:210437/100‑1 (MQ=255) cATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGAcc < 1:184524/100‑1 (MQ=255) cATTCGTATCATCCCTGAATGTCACTGCCAAAGGCTGACAATAACCAAAGCAGCTATCGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGAcc < 2:777960/100‑1 (MQ=255) aTTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCa < 1:721241/100‑1 (MQ=255) ggTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCAATa > 2:429418/1‑100 (MQ=255) | CGTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCAATA > NC_000913/2183559‑2183752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |