| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,306,399 | C→T | W119* (TGG→TAG) | mqo ← | malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,306,399 | 0 | C | T | 96.3% | 175.8 / ‑1.7 | 54 | W119* (TGG→TAG) | mqo | malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (26/26); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
ACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTC > NC_000913/2306306‑2306493 | aCGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACt > 2:526675/1‑100 (MQ=255) ccTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGa > 1:913285/1‑100 (MQ=255) cTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGaa > 1:344752/1‑100 (MQ=255) gCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATc < 1:295273/100‑1 (MQ=255) ccccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCt < 1:132907/100‑1 (MQ=255) ccccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCt > 2:401496/1‑100 (MQ=255) ccccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCt > 1:940662/1‑100 (MQ=255) cccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTg < 2:923725/100‑1 (MQ=255) cccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTg < 2:273741/100‑1 (MQ=255) cccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTg > 1:290107/1‑100 (MQ=255) ccAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGa > 2:69141/1‑100 (MQ=255) aGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGaaa < 1:547377/100‑1 (MQ=255) cAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGc > 2:890950/1‑100 (MQ=255) aaGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCtt > 1:1186954/1‑100 (MQ=255) aaGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCtt > 1:526441/1‑100 (MQ=255) gCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCg > 2:127502/1‑100 (MQ=255) cTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGt > 1:81596/1‑100 (MQ=255) cTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGt < 1:642430/100‑1 (MQ=255) tCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGtt > 1:170969/1‑100 (MQ=255) tCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGtt > 1:246281/1‑100 (MQ=255) cATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTa > 2:718735/1‑100 (MQ=255) atGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATg < 2:445894/100‑1 (MQ=255) tGCGGAACGGTATTGATAAATGAACGCGGCGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATgg < 2:787086/100‑1 (MQ=255) cGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCg < 2:58183/100‑1 (MQ=255) cGGAACGGTATTGATAAATGAACGAAGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTCGAAGTGGCGGGAAATCTGAAATGCTTCGTTAATgg > 2:80565/1‑98 (MQ=255) aaCGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACt < 1:69142/100‑1 (MQ=255) aaCGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACt < 1:436507/100‑1 (MQ=255) aTTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt < 1:890957/100‑1 (MQ=255) aTTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt > 2:314715/1‑100 (MQ=255) aTTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt > 2:81238/1‑100 (MQ=255) gATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCa < 1:498106/100‑1 (MQ=255) gATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCa < 1:178433/100‑1 (MQ=255) tAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAAt < 1:268410/100‑1 (MQ=255) tAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAAt > 1:664189/1‑100 (MQ=255) aaaTGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATa > 1:1048125/1‑100 (MQ=255) aaCGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGCAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGAt > 1:1290218/1‑100 (MQ=255) aCGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCg < 1:750972/100‑1 (MQ=255) cGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGTGAAATCTGAAATGCTTCGTGAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCgg > 2:709885/1‑100 (MQ=255) cGCAGCACGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCATTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCgg > 2:184391/1‑100 (MQ=255) gcagcaCGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGc < 1:785499/100‑1 (MQ=255) agcaCGCCGCGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGt < 2:253043/100‑1 (MQ=255) ccgcGCTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCg < 2:1141693/100‑1 (MQ=255) gcgcTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCggg < 2:1179402/100‑1 (MQ=255) gcgcTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCggg < 1:640074/100‑1 (MQ=255) cgcTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCgggg > 1:547382/1‑100 (MQ=255) cTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtg < 1:49319/100‑1 (MQ=255) cTCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtg > 2:766128/1‑100 (MQ=255) tCAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtgt < 1:1196479/100‑1 (MQ=255) cAACCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtgt > 2:102638/1‑99 (MQ=255) aCCTGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagt > 2:578250/1‑100 (MQ=255) tGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttca < 2:919135/100‑1 (MQ=255) tGGTGCGCCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttca < 2:940654/100‑1 (MQ=255) gcgcCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagttc < 2:913277/100‑1 (MQ=255) gcgcCTAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagtt > 2:834749/1‑99 (MQ=255) | ACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTC > NC_000913/2306306‑2306493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |