Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,018,260 (A)8→7 100% coding (315/444 nt) fliJ → flagellar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,018,2530A.92.8% 494.0 / 26.8 125coding (308/444 nt)fliJflagellar protein
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base . (69/47);  total (73/52)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348
                                                                                                   |                                                                                               
agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  2:3129290/1‑100 (MQ=255)
agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAAAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  1:516976/1‑100 (MQ=255)
 gcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaa                                                                                                >  2:3777499/1‑100 (MQ=255)
  cagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaa                                                                                               >  2:1241784/1‑100 (MQ=255)
   agTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaa                                                                                              <  2:3308730/100‑1 (MQ=255)
   agTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaa                                                                                               >  2:838934/1‑99 (MQ=255)
     tttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaa                                                                                            <  2:790895/100‑1 (MQ=255)
      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           <  2:805390/100‑1 (MQ=255)
      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           <  1:1006728/100‑1 (MQ=255)
      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGAAGAAAAGaaaaaaa                                                                                           <  1:3510449/100‑1 (MQ=255)
         tCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaa                                                                                        >  2:1784285/1‑97 (MQ=255)
          ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       >  1:2916101/1‑96 (MQ=255)
          ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACCTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       >  1:2835220/1‑96 (MQ=255)
            aGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGt                                                                                    >  1:226836/1‑100 (MQ=255)
              aCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGttt                                                                                  <  2:151476/100‑1 (MQ=255)
               cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTg                                                                                 >  2:612162/1‑100 (MQ=255)
                 cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCa                                                                               >  1:2176157/1‑100 (MQ=255)
                  tGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAg                                                                              >  2:524815/1‑100 (MQ=255)
                   ggAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAgg                                                                             <  1:3686479/100‑1 (MQ=255)
                    gAAAATGTCATTACTTAGCATCGCCAGCAACTTAATCAGTGGACGCATAAAGTTGACAGTGCCCTGAACAGTTGGCGAG‑CAAAAAGCAACGTTTGCa                                                                               >  1:1617855/1‑97 (MQ=255)
                    gAAAAAGCCAATATTCAGCATCGCCAGCAACTTAATCAGTGGACACAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGc                                                                            >  1:3504169/1‑100 (MQ=255)
                       aaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTg                                                                         <  2:910354/100‑1 (MQ=255)
                       aaaGCCATAAATCAGCATCGCCAGCAACATAATCAGTGGAAAAAGAAAGTTGACATTGCCCTGAAAAAAAAAAAA‑AAAAAAAACAACGTTTGCAGGCCTg                                                                         <  1:3694946/100‑1 (MQ=255)
                        aaGCCATTACCCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTgg                                                                        >  1:387134/1‑100 (MQ=255)
                         agcaataaatCAGAATCACCAACAACTTAAACAGTAAAAAAAGAAAGTTGACATTACCCTTAAAAAAAAAAAA‑AAAAAAAACAACGTTTGCAGGCCTGGc                                                                       <  1:1368691/91‑1 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGc                                                                       >  2:1849973/1‑100 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGc                                                                       >  1:3034046/1‑100 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGc                                                                       <  2:396884/100‑1 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACACTTGGAGAG‑AACAACACCACGTATGAAGGCCtcca                                                                       >  2:1640459/1‑97 (MQ=255)
                            cATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGa                                                                    >  2:2239547/1‑100 (MQ=255)
                             aTTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGac                                                                   <  2:1317667/100‑1 (MQ=255)
                               tACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGacac                                                                 <  1:1845787/100‑1 (MQ=255)
                                aCTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCGCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACt                                                                >  1:778556/1‑100 (MQ=255)
                                aCTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACt                                                                >  1:398726/1‑100 (MQ=255)
                                 cTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTg                                                               >  2:2046729/1‑100 (MQ=255)
                                  tCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              <  2:1935245/100‑1 (MQ=255)
                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             >  1:803578/1‑100 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAA‑AACGTTTGCAGGCCTGGCAGACACTGCAg                                                            >  1:2885326/1‑100 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            >  2:3445528/1‑100 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            >  1:1961243/1‑100 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            <  2:1304818/100‑1 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTCACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACCTGTGCAGCCTCGGGAGacac                                                                 >  1:2885185/1‑95 (MQ=255)
                                    aGCATCGCCAGCAACCTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            <  2:3463127/100‑1 (MQ=255)
                                    aGCATCGCCAGCAAATTAATCAGGGGAGGCAGAAAGTTGACAGTGCCCGGAACAGATGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              <  1:2962979/98‑1 (MQ=255)
                                       aTCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAACAACAACGTTTGCAGGCCTGGCAGACACTGCAGGaa                                                         >  1:1714390/1‑100 (MQ=255)
                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAAc                                                        >  1:315148/1‑99 (MQ=255)
                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       <  2:2362753/100‑1 (MQ=255)
                                         cGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       <  2:1580047/100‑1 (MQ=255)
                                          gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAgatcgg                                                       >  1:821770/1‑95 (MQ=255)
                                          gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       >  1:1886630/1‑99 (MQ=255)
                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  2:2027407/100‑1 (MQ=255)
                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     >  2:1780514/1‑100 (MQ=255)
                                                 aCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCa                                               >  2:3094021/1‑100 (MQ=255)
                                                  cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              <  1:52305/100‑1 (MQ=255)
                                                  cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              >  1:2194134/1‑100 (MQ=255)
                                                   ttAATCGGAGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACACCGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              >  1:2661137/1‑99 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  1:233336/1‑100 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  2:2403528/1‑100 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            <  1:10435/100‑1 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  1:1901501/1‑100 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  1:1543244/1‑100 (MQ=255)
                                                     aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggc                                           >  2:3031655/1‑100 (MQ=255)
                                                      aTCAGTGGACGCAGATAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggcg                                          <  2:3753809/100‑1 (MQ=255)
                                                      aTCAGTGGACGCAGAAAGTTGACATTGCCCTGAAAAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggcg                                          <  1:1117943/100‑1 (MQ=255)
                                                        cAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        >  1:1797057/1‑100 (MQ=255)
                                                        cAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        <  1:2760699/100‑1 (MQ=255)
                                                         aGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCa                                       >  2:335568/1‑100 (MQ=255)
                                                         aGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCa                                       >  1:2102736/1‑100 (MQ=255)
                                                          gTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCAc                                      >  1:677234/1‑100 (MQ=255)
                                                            ggACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTg                                    >  1:2822105/1‑100 (MQ=255)
                                                            ggACGAAGAAAGTTGACATTGCCCTGAACAGTTGGCGA‑AAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTg                                    <  1:510464/100‑1 (MQ=255)
                                                             gACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGc                                   <  2:654861/100‑1 (MQ=255)
                                                             gACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGc                                   >  2:2888665/1‑100 (MQ=255)
                                                              aCGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCt                                  <  2:3451784/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:2439970/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:363427/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               >  1:1470467/1‑100 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCGGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  1:2246711/100‑1 (MQ=255)
                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  2:2864155/100‑1 (MQ=255)
                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             >  2:3201174/1‑100 (MQ=255)
                                                                    aaaGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGa                            <  2:2182004/100‑1 (MQ=255)
                                                                       gTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaaa                         <  1:955990/100‑1 (MQ=255)
                                                                       gTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaa                          <  1:3288937/99‑1 (MQ=255)
                                                                       attcaCATTGCCCTGAACAGCTGGCGA‑TAAAAAAACATCGTTTGCAGGCGTGGCAGACACTGCAGGAACGCCAAGCCACGGCCGCACTGCTTGCCGa                            >  1:1763246/5‑97 (MQ=255)
                                                                        ttGACAGTGCCCTGAACAGTTGGCGA‑TAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaaa                         <  1:3282356/99‑1 (MQ=255)
                                                                           aCATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGc                     <  1:321865/100‑1 (MQ=255)
                                                                            cATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGcc                    >  2:1649862/1‑100 (MQ=255)
                                                                               tGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCg                 <  1:2893405/100‑1 (MQ=255)
                                                                                 cccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               <  2:1883210/100‑1 (MQ=255)
                                                                                 cccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               <  2:12923/100‑1 (MQ=255)
                                                                                  ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              >  2:651444/1‑100 (MQ=255)
                                                                                  ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCATGCAGAAAACCGCCTCGATc              <  1:2326856/100‑1 (MQ=255)
                                                                                   cTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCg                 <  1:3537315/96‑1 (MQ=255)
                                                                                        cAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        <  2:2848148/100‑1 (MQ=255)
                                                                                        cAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        >  1:583377/1‑100 (MQ=255)
                                                                                          gTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAg      >  2:3684747/1‑100 (MQ=255)
                                                                                            tGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgat    <  2:567155/100‑1 (MQ=255)
                                                                                             ggCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCATACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgat    <  1:1999668/99‑1 (MQ=255)
                                                                                             ggCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCATAAAACCGCCTCGATCAGAAAAAgatg   >  1:2585922/1‑100 (MQ=255)
                                                                                              gCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  2:2093124/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑