Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258676 258683 8–776 134 [0] [0] 132 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)109 (0.860) 67/162 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 132 total aligned reads displayed.

AACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCT  >  NC_000913/258587‑258783
                                                                                                 |                                                                                                   
tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtacattcgtgaaggtaAg                                                                                                    >  1:2219791‑M2/98‑99 (MQ=255)
  acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 <  1:3688508‑M2/5‑1 (MQ=255)
  acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 >  1:2234496‑M2/96‑100 (MQ=255)
   caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                 <  2:1393330‑M2/5‑1 (MQ=255)
     acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                              >  2:944583‑M2/93‑100 (MQ=255)
     acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                              <  2:1029082‑M2/8‑1 (MQ=255)
       aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                            <  1:3043803‑M2/10‑1 (MQ=255)
          agatagcaatgacgttaccgagtggacacccgaagagaaaaataaaaaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATa                                                                                          <  1:751326‑M2/12‑1 (MQ=255)
            atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                       <  1:1151931‑M2/15‑1 (MQ=255)
            atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                       <  2:3312974‑M2/15‑1 (MQ=255)
            atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                       <  1:2179810‑M2/15‑1 (MQ=255)
             tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                       >  2:3505740‑M2/85‑99 (MQ=255)
             tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                      <  1:1436262‑M2/16‑1 (MQ=255)
              agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                     >  1:762097‑M2/84‑100 (MQ=255)
               gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                    >  1:2796653‑M2/83‑100 (MQ=255)
                caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                   <  2:476955‑M2/19‑1 (MQ=255)
                caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                   <  2:617066‑M2/19‑1 (MQ=255)
                 aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                  >  2:2261624‑M2/81‑100 (MQ=255)
                  atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgctctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                 <  1:2105945‑M2/21‑1 (MQ=255)
                  atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                 <  2:3222451‑M2/21‑1 (MQ=255)
                      cgttaccgagtggacacccgaagagaagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                             >  1:46807‑M2/76‑100 (MQ=255)
                       gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                            <  1:248452‑M2/26‑1 (MQ=255)
                       gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                            >  2:812696‑M2/75‑100 (MQ=255)
                       gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAATGCAAAGATAATCGATTCtttt                                                                              >  1:592336‑M2/75‑98 (MQ=255)
                        ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                           <  2:1161365‑M2/27‑1 (MQ=255)
                           ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                        >  1:1470707‑M2/71‑100 (MQ=255)
                            cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                       >  1:2361446‑M2/70‑100 (MQ=255)
                            cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                       >  1:3453851‑M2/70‑100 (MQ=255)
                              agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                     <  2:1188369‑M2/33‑1 (MQ=255)
                              agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                     >  1:1346925‑M2/68‑100 (MQ=255)
                                 ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                  >  2:1836283‑M2/65‑100 (MQ=255)
                                 ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                  >  1:1025242‑M2/65‑100 (MQ=255)
                                 ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAACCGATTCTTttttgtttgttt                                                                    >  1:1213098‑M2/65‑96 (MQ=255)
                                  gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                 <  2:1415704‑M2/37‑1 (MQ=255)
                                  gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                 <  2:879‑M2/37‑1 (MQ=255)
                                   acacccgaagagcagattgatcaaaaaatataccgcactaggcccgtatattcgtgtaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                 <  1:2051053‑M2/37‑1 (MQ=255)
                                    cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                               <  2:2631601‑M2/39‑1 (MQ=255)
                                    cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                               >  2:3148574‑M2/62‑100 (MQ=255)
                                      cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             <  2:1736520‑M2/41‑1 (MQ=255)
                                      cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             >  1:1913910‑M2/60‑100 (MQ=255)
                                       ccgaagagcagattgattaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                             <  1:3295644‑M2/41‑1 (MQ=255)
                                       ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                            >  2:1156606‑M2/59‑100 (MQ=255)
                                        cgaagagcagattgatcaaaaaatttaccgcattaggccagtctattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATg                                                           <  1:2665613‑M2/43‑1 (MQ=255)
                                          aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                         <  2:1675264‑M2/45‑1 (MQ=255)
                                          aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                         <  1:1184766‑M2/45‑1 (MQ=255)
                                           agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                        >  2:2472583‑M2/55‑100 (MQ=255)
                                            gagcagattgatcaaaaaatttaccgcactaggcccgtctattcgtgaaggtaAGTGCGAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                       <  1:2065277‑M2/47‑1 (MQ=255)
                                             agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                      <  2:213331‑M2/48‑1 (MQ=255)
                                              gcagattgatcaaaaaatttaccccactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                      <  1:814376‑M2/48‑1 (MQ=255)
                                                 gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                  <  1:2743383‑M2/52‑1 (MQ=255)
                                                  attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                 <  1:3724605‑M2/53‑1 (MQ=255)
                                                   ttgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                <  2:2622845‑M2/54‑1 (MQ=255)
                                                    tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                               <  2:2610622‑M2/55‑1 (MQ=255)
                                                     gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                              >  2:932864‑M2/45‑100 (MQ=255)
                                                     gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                              >  1:2007946‑M2/45‑100 (MQ=255)
                                                     gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                              >  2:2890889‑M2/45‑100 (MQ=255)
                                                      atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                               >  2:635101‑M2/44‑98 (MQ=255)
                                                      atcaaaaaatttaccgcactaggcccgtatattcgtgaagggaAGTGCAAAGATAATCGACTCTTTTTCGATTTTCTGGTTGTATGCGTCAACGTCAAAc                                             >  1:1282739‑M2/44‑100 (MQ=255)
                                                       tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                            <  2:1551041‑M2/58‑1 (MQ=255)
                                                        caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTTCTGTATGCGTCAACGTGAAACCg                                           >  1:587181‑M2/42‑100 (MQ=255)
                                                        caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAaccn                                           <  2:2915877‑M2/59‑2 (MQ=255)
                                                        caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                           >  1:109897‑M2/42‑100 (MQ=255)
                                                        caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                           >  2:1438571‑M2/42‑100 (MQ=255)
                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTTTGCGTCAACGTGAAACCgg                                          >  1:2824742‑M2/41‑100 (MQ=255)
                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                          >  2:1774247‑M2/41‑100 (MQ=255)
                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                          <  1:2731426‑M2/60‑1 (MQ=255)
                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTATTTTTCGATTGTTTGGATGTATGAGTGAACGTGAAACCgg                                          >  1:2713799‑M2/41‑100 (MQ=255)
                                                          aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                         <  2:3558562‑M2/61‑1 (MQ=255)
                                                          aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                         <  1:2712527‑M2/61‑1 (MQ=255)
                                                          aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                         >  2:490349‑M2/40‑100 (MQ=255)
                                                           aaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCa                                        <  1:2412688‑M2/62‑1 (MQ=255)
                                                                 taccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                  <  2:21766‑M2/68‑1 (MQ=255)
                                                                  accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                 <  2:184840‑M2/69‑1 (MQ=255)
                                                                  accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                 <  1:2465765‑M2/69‑1 (MQ=255)
                                                                    cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                               <  2:3183484‑M2/71‑1 (MQ=255)
                                                                     gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                              >  2:3390688‑M2/29‑100 (MQ=255)
                                                                      cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                             >  2:2107632‑M2/28‑100 (MQ=255)
                                                                         taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGTGTCAACGTGAAACCGGCACCGGAAGTGCGt                            <  1:2910930‑M2/74‑1 (MQ=255)
                                                                         taggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGa                          <  1:1979868‑M2/76‑1 (MQ=255)
                                                                           ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                        <  1:3414012‑M2/78‑1 (MQ=255)
                                                                             cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                      <  2:2047216‑M2/80‑1 (MQ=255)
                                                                             cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                      >  1:2376193‑M2/21‑100 (MQ=255)
                                                                             cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                      >  2:293155‑M2/21‑100 (MQ=255)
                                                                               cgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                    <  1:3111880‑M2/82‑1 (MQ=255)
                                                                                gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                   <  1:3722814‑M2/83‑1 (MQ=255)
                                                                                gtatattcgtgaaggtaAGTGCAAAGAGAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGGAACCGGCACCGGAAGTGCGTGACTTCTgg                   <  1:2854517‑M2/83‑1 (MQ=255)
                                                                                  atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                 <  1:3658547‑M2/85‑1 (MQ=255)
                                                                                  atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                 <  1:3278530‑M2/85‑1 (MQ=255)
                                                                                   tattcgtgaaggtaAGTGCAAAGATAATCGATTTTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                 <  1:1274157‑M2/85‑1 (MQ=255)
                                                                                     ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg              <  2:409728‑M2/88‑1 (MQ=255)
                                                                                      tcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg             <  1:3474711‑M2/89‑1 (MQ=255)
                                                                                        gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg           <  2:2250849‑M2/91‑1 (MQ=255)
                                                                                        gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg           <  2:769444‑M2/91‑1 (MQ=255)
                                                                                          gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga         <  1:962089‑M2/93‑1 (MQ=255)
                                                                                            aggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGCGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg       <  1:944578‑M2/95‑1 (MQ=255)
                                                                                              gtaAGTGGAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga     >  2:1997556‑M2/4‑100 (MQ=255)
                                                                                               taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg    >  2:3697258‑M2/3‑100 (MQ=255)
                                                                                                aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc   <  2:1470713‑M2/99‑1 (MQ=255)
                                                                                                aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc   <  2:1467008‑M2/99‑1 (MQ=255)
                                                                                                 aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt  >  1:755131/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
AACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCT  >  NC_000913/258587‑258783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: