Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,129,394 G→C 15.4% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,3940GC15.4% 489.6 / 72.4 176intergenic (+20/+20)murJ/flgNputative lipid II flippase/export chaperone for FlgK and FlgL
Reads supporting (aligned to +/- strand):  ref base G (86/61);  new base C (14/13);  total (102/74)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 198 total aligned reads displayed.

TGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCG  >  NC_000913/1129295‑1129493
                                                                                                   |                                                                                                   
 ggCGGGGATTGGCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGGTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt                                                                                                    <  1:287061/100‑1 (MQ=255)
  gCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagn                                                                                                    <  1:1226844/99‑2 (MQ=255)
   cGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc                                                                                                  >  2:2074973/1‑100 (MQ=255)
   cGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc                                                                                                  >  2:1538820/1‑100 (MQ=255)
     gggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag                                                                                                <  2:983323/100‑1 (MQ=255)
     gggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag                                                                                                <  2:1176601/100‑1 (MQ=255)
       gATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGc                                                                                              >  2:448536/1‑100 (MQ=255)
       gATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATATGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGc                                                                                              <  1:2110877/100‑1 (MQ=255)
       gATTGCCGCGTACTTCGCTGCACGGGCGGTACTGGGCTTCAACGTTAAAGAAGTTGCCCGCCGGACGGTGTCACAATGCATTCCGGCCtgcagtgc                                                                                                  >  1:760642/1‑96 (MQ=255)
        aTTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGcc                                                                                             <  2:3653917/100‑1 (MQ=255)
        aTTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGcc                                                                                             <  2:1015652/100‑1 (MQ=255)
          tGCCGGGTACTGCGCTGCACTGGCGGTACTGGGCGTCAAAGTTAAAGCACGGGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                           <  1:2628802/100‑1 (MQ=255)
          tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                           <  1:1739193/100‑1 (MQ=255)
           gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGga                                                                                          <  2:646634/100‑1 (MQ=255)
           gCCGCGTACTTCGCGGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATATGCCCGCCGGCCGGTGCGACAATTCATTCCGACCTGCAGTGCAGGCCgg                                                                                           >  1:1417058/1‑99 (MQ=255)
             cgcgTACTTCGCTGCACTGGCGGTACTGGGCTCCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaat                                                                                        <  1:3446880/100‑3 (MQ=255)
               cgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaatgc                                                                                      <  2:1436197/100‑5 (MQ=255)
               cgTAATTCGCTGCACTGGCGGTAATGGGCTCAAAAGTTAAAGAATTTGACCGCCAGACGGTGTAACAATGCATTCCGGTCTGCACGGCTGGCCGga                                                                                          <  1:1995886/96‑1 (MQ=255)
                   cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCt                                                                                  >  1:1063260/1‑100 (MQ=255)
                      cGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                               <  1:78113/100‑1 (MQ=255)
                      cGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                               <  1:1666409/100‑1 (MQ=255)
                      cGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                               <  2:1945266/100‑1 (MQ=255)
                       gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAg                                                                              >  2:726050/1‑100 (MQ=255)
                       gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAg                                                                              >  1:380463/1‑100 (MQ=255)
                       gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCAGGACGGTGTAACAATGCATTCCCGCCTGCAATTCAGGCCAGAAATAATCATCAg                                                                              >  1:729964/1‑100 (MQ=255)
                        cTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGa                                                                             >  2:2604130/1‑100 (MQ=255)
                         tGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGAt                                                                            <  1:3539498/100‑1 (MQ=255)
                          gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc                                                                           >  1:146748/1‑100 (MQ=255)
                           cACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCg                                                                          <  2:2404010/100‑1 (MQ=255)
                            aCTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCg                                                                          >  1:3623302/1‑99 (MQ=255)
                            aCTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGa                                                                         >  2:3525992/1‑100 (MQ=255)
                               ggCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt                                                                      >  2:2353013/1‑100 (MQ=255)
                               ggCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt                                                                      >  1:839608/1‑100 (MQ=255)
                                   gTACTGGGCTTCAAATTTAAAGAATTTGCCCGCCGGACGTTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttt                                                                  <  1:1439569/100‑1 (MQ=255)
                                     aCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                >  1:290610/1‑100 (MQ=255)
                                      cTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTa                                                               >  1:176157/1‑100 (MQ=255)
                                        gggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAcc                                                             >  1:2515238/1‑100 (MQ=255)
                                           cTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgcc                                                          >  2:941582/1‑100 (MQ=255)
                                            ttCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTACAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgccg                                                         <  1:1078278/100‑1 (MQ=255)
                                             tCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACcgccgc                                                        >  2:3188152/1‑100 (MQ=255)
                                              cAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCcgcg                                                       <  1:2393/100‑1 (MQ=255)
                                              cAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCcgcg                                                       <  2:2807554/100‑1 (MQ=255)
                                                aaGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAt                                                     >  1:3658286/1‑100 (MQ=255)
                                                  gTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATgg                                                   <  2:888177/100‑1 (MQ=255)
                                                  gTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATgg                                                   >  2:1980400/1‑100 (MQ=255)
                                                  gTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATgg                                                   >  1:1843818/1‑100 (MQ=255)
                                                   ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCAGCGATggg                                                  >  1:2403429/1‑100 (MQ=255)
                                                    tAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgt                                                 >  2:360710/1‑100 (MQ=255)
                                                       aGAATTTGCCCGCCGGACGGTGTAACAATGCATTCAGGCATGCAGTGCAGGACGGAGATAATCTTCAGATCAAAATCTTTTTACCGCCGCGATGGgttgt                                              >  1:3297998/1‑100 (MQ=255)
                                                        gAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgtt                                             >  1:3570763/1‑100 (MQ=255)
                                                        gAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgtt                                             <  2:1512462/100‑1 (MQ=255)
                                                             tGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGa                                           >  1:1890817/1‑97 (MQ=255)
                                                               cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGta                                       >  1:2305333/1‑98 (MQ=255)
                                                               cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                      <  2:333837/100‑1 (MQ=255)
                                                               cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                      >  2:1880395/1‑100 (MQ=255)
                                                               cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                      >  2:3770312/1‑100 (MQ=255)
                                                               cccGCCGGACGGTGTAACAATGCATTACGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                      >  2:1131858/1‑100 (MQ=255)
                                                                 cgccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGa                                    <  2:2327736/100‑1 (MQ=255)
                                                                   ccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAcc                                  >  1:3757591/1‑100 (MQ=255)
                                                                      gACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGtt                               >  2:769276/1‑100 (MQ=255)
                                                                        cGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCg                             <  1:2808500/100‑1 (MQ=255)
                                                                        cGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCg                             <  2:2166177/100‑1 (MQ=255)
                                                                        cGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAAGCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTc                              <  1:862142/99‑1 (MQ=255)
                                                                        cGGTGTAACAATGAATTCCGACATGCAGTGCAGGCCGGAGATAATATTCAGATCGAAATCATTTTAACGCCGAGATGGGTTGATAAGGTCTGACCGtt                               >  1:3078318/1‑98 (MQ=255)
                                                                          gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc                           <  2:3063312/100‑1 (MQ=255)
                                                                          gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc                           <  2:1098949/100‑1 (MQ=255)
                                                                           tgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGccc                          <  2:371410/100‑1 (MQ=255)
                                                                            gtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcccc                         <  1:518274/100‑1 (MQ=255)
                                                                             tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa                        <  2:2310152/100‑1 (MQ=255)
                                                                             tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa                        >  2:1817689/1‑100 (MQ=255)
                                                                              aaaaaTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCat                       <  1:873375/97‑1 (MQ=255)
                                                                               aCAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCata                      <  2:455771/100‑1 (MQ=255)
                                                                                 aaTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCAtata                    >  2:191782/1‑100 (MQ=255)
                                                                                  aTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAg                   >  2:525857/1‑100 (MQ=255)
                                                                                   tGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGc                  <  2:582931/100‑1 (MQ=255)
                                                                                   tGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGc                  >  2:1346136/1‑100 (MQ=255)
                                                                                     cATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGt                >  1:2962481/1‑100 (MQ=255)
                                                                                      tctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTc               >  2:2039708/3‑100 (MQ=255)
                                                                                      tctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGACGGCTTGTTGAGGTCTGACCGTTCCCCCCATATAGCGTc               >  1:3493343/3‑100 (MQ=255)
                                                                                      aTTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTc               >  2:3543092/1‑100 (MQ=255)
                                                                                      aTTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTc               <  2:811334/100‑1 (MQ=255)
                                                                                       ctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTc               >  1:1850638/2‑99 (MQ=255)
                                                                                        tCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCgg             >  2:961889/1‑100 (MQ=255)
                                                                                        tCCGGCCCGAATTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGAGATGTTGAGGTCTGACCTTTCCCCAAATATAGAGTCgg             >  1:1982090/1‑100 (MQ=255)
                                                                                         ccGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGt            >  2:1686171/1‑100 (MQ=255)
                                                                                         ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGt            >  2:559353/1‑100 (MQ=255)
                                                                                           ggCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGCGCGTTCGCCCCATATAGGGTCGGTTc          >  2:3382232/1‑100 (MQ=255)
                                                                                           ggCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTc          >  2:1748659/1‑100 (MQ=255)
                                                                                           ggCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTc          >  2:2731720/1‑100 (MQ=255)
                                                                                           ggCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGt            >  1:1776116/1‑98 (MQ=255)
                                                                                             ccTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt        <  1:448534/100‑1 (MQ=255)
                                                                                             ccTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt        <  2:2709832/100‑1 (MQ=255)
                                                                                              cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg       >  2:34424/1‑100 (MQ=255)
                                                                                              cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg       <  2:290612/100‑1 (MQ=255)
                                                                                              cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg       <  1:2766288/100‑1 (MQ=255)
                                                                                               tgcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGa      >  1:1942170/1‑100 (MQ=255)
                                                                                               tgcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGa      <  2:2844043/100‑1 (MQ=255)
                                                                                               tgcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGa      >  2:1618593/1‑100 (MQ=255)
                                                                                                gcagtggagGCCGGAGATAATCTTCAGCTCGAAATCATTTTCCCGCCGCGATGGGTTGTTGGGGTGTGACCGTTCGCGCCATATAGCGTCGGTTCCt        <  1:1194462/97‑1 (MQ=255)
                                                                                                gcactgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGAt     >  2:1496941/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCG  >  NC_000913/1129295‑1129493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: