Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,143,641 G→A 100% H243Y (CAT→TAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,6410GA98.3% 448.9 / ‑2.3 137H243Y (CAT→TAT) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (69/64);  total (70/67)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143546‑1143736
                                                                                               |                                                                                               
cTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGa                                                                                             <  1:3513231/100‑1 (MQ=255)
 tgtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGGGTACAGTTTGATTTTGCTGCTGAAATCCGGGGGACCTAATGCAGCGATATACTGa                                                                                             >  1:3699055/1‑99 (MQ=255)
    gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         >  2:3061043/1‑100 (MQ=255)
    gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         <  2:2274656/100‑1 (MQ=255)
     aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGc                                                                                        <  2:408634/100‑1 (MQ=255)
      cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  1:3137054/100‑1 (MQ=255)
        cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAg                                                                                     >  1:1278748/1‑100 (MQ=255)
        cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     <  1:2145933/100‑1 (MQ=255)
         gATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGt                                                                                    >  2:1314544/1‑100 (MQ=255)
           tCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTc                                                                                  >  1:1211264/1‑100 (MQ=255)
            cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 <  1:3379490/100‑1 (MQ=255)
            cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGAGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCAAGTTCg                                                                                 >  1:434254/1‑100 (MQ=255)
              gggaGTGGCGCAACAGCGTGATCTCGCCGGTGTCGAGTTTGATTTTCCTGCGGACATCCGGGCGAACTAATGCAGCCATATTCTGACGTGCCAGTTCGa                                                                                <  1:425683/96‑1 (MQ=255)
              ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:592347/100‑1 (MQ=255)
              ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               >  2:1504119/1‑100 (MQ=255)
               gTAGTGGCTGAACAGCGGGATCTCGCTGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGCCCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 <  1:2275324/97‑1 (MQ=255)
               gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc                                                                              <  2:2283465/100‑1 (MQ=255)
               gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc                                                                              <  1:61204/100‑1 (MQ=255)
                  gTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACt                                                                           <  1:1658348/100‑1 (MQ=255)
                   tGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACtt                                                                          >  1:248490/1‑100 (MQ=255)
                    ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  1:894145/1‑100 (MQ=255)
                     gCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTc                                                                        >  1:2789909/1‑100 (MQ=255)
                      cTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCg                                                                       >  1:2233773/1‑100 (MQ=255)
                        gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     <  1:1576349/100‑1 (MQ=255)
                        gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     <  1:2456343/100‑1 (MQ=255)
                        gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAAGCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     <  2:3428008/100‑1 (MQ=255)
                          aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     >  2:1490506/1‑98 (MQ=255)
                           cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  >  2:2626760/1‑100 (MQ=255)
                           cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  >  1:1182922/1‑100 (MQ=255)
                             gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGCGCACTTTCGGGTTATc                                                                >  1:1226746/1‑100 (MQ=255)
                             gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:3104953/100‑1 (MQ=255)
                             gCGGGATCTCGCCGGTGTACAGTTTGATTGTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  1:1077849/100‑1 (MQ=255)
                               gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                              <  1:1499811/100‑1 (MQ=255)
                               gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                              <  2:571942/100‑1 (MQ=255)
                               gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCAGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGGTATCGa                                                              >  1:1044356/1‑100 (MQ=255)
                               gggATCGCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                              <  1:1625949/100‑1 (MQ=255)
                                 gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa                                                            >  2:538306/1‑100 (MQ=255)
                                    ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAgg                                                         >  2:1725036/1‑100 (MQ=255)
                                    ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAgg                                                         >  2:1516411/1‑100 (MQ=255)
                                      cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt                                                       >  1:328463/1‑100 (MQ=255)
                                       gCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAtt                                                      >  2:2344124/1‑100 (MQ=255)
                                       gCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAtt                                                      <  1:2005350/100‑1 (MQ=255)
                                       gCCGGTGCACACTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGACAGGGa                                                        >  1:1994962/1‑98 (MQ=255)
                                          gttgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   <  1:3579622/98‑1 (MQ=255)
                                          ggTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   >  1:1154825/1‑100 (MQ=255)
                                            tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc                                                 <  1:2839169/100‑1 (MQ=255)
                                            tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc                                                 <  2:399037/100‑1 (MQ=255)
                                            tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc                                                 <  2:3482786/100‑1 (MQ=255)
                                             gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg                                                <  2:2333210/100‑1 (MQ=255)
                                              tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               <  1:1644283/100‑1 (MQ=255)
                                              tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               <  1:3056106/100‑1 (MQ=255)
                                              gaCAGTGTGAGTTGGCTGGTGAAATCCGGGTGACCTAATGGAGCGGTATACTGACGTGCCAGTGCGAGTACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               <  1:3206197/99‑1 (MQ=255)
                                                cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             <  1:2716045/100‑1 (MQ=255)
                                                  gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGCTATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                           >  2:3234508/1‑100 (MQ=255)
                                                  gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                           >  2:510857/1‑100 (MQ=255)
                                                  gTTTGATTTTGCTGCGGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                           >  1:1040523/1‑100 (MQ=255)
                                                   tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  2:2095102/100‑1 (MQ=255)
                                                   tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  1:1893932/100‑1 (MQ=255)
                                                    ttGATTTTGCTGGTGAGATCCGGGCGAACTGATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:1673687/100‑1 (MQ=255)
                                                    ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  1:144421/99‑1 (MQ=255)
                                                    ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:2389746/100‑1 (MQ=255)
                                                    ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         >  1:2731549/1‑100 (MQ=255)
                                                     tGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTg                                        >  1:1989672/1‑100 (MQ=255)
                                                      gATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTCACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa                                       <  1:998014/100‑1 (MQ=255)
                                                        ttttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACg                                     >  2:2047555/1‑100 (MQ=255)
                                                         tttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgt                                    >  1:929585/1‑100 (MQ=255)
                                                          ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgta                                   <  2:1645088/100‑1 (MQ=255)
                                                          ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgta                                   <  2:66227/100‑1 (MQ=255)
                                                           tgctgcTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaa                                  >  1:1920226/1‑100 (MQ=255)
                                                             ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                <  1:3723127/100‑1 (MQ=255)
                                                             ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                >  2:1803387/1‑100 (MQ=255)
                                                             ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                >  2:1426731/1‑100 (MQ=255)
                                                             ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                >  2:1338294/1‑100 (MQ=255)
                                                              tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  1:1175545/1‑100 (MQ=255)
                                                              tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  1:435390/1‑100 (MQ=255)
                                                                ctgAAGTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt                             >  1:2849559/1‑100 (MQ=255)
                                                                 tgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATc                            >  2:2349964/1‑100 (MQ=255)
                                                                  gAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcg                           <  1:239873/100‑1 (MQ=255)
                                                                       ccGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      <  2:1278753/100‑1 (MQ=255)
                                                                       ccGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      >  1:1711578/1‑100 (MQ=255)
                                                                        cGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt                     <  2:2896628/100‑1 (MQ=255)
                                                                         gggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg                    <  2:2358989/100‑1 (MQ=255)
                                                                           gcaaCCTAATCCAGCGATATACTGACGGGCCATTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  <  1:2773239/97‑1 (MQ=255)
                                                                            cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcgc                 >  2:3282534/1‑100 (MQ=255)
                                                                             gACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGcgcg                <  2:3191906/100‑1 (MQ=255)
                                                                              aCCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGa               >  1:94383/1‑100 (MQ=255)
                                                                              aCCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGa               >  2:61984/1‑100 (MQ=255)
                                                                               ccTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa              <  2:3015288/100‑1 (MQ=255)
                                                                               ccTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa              >  1:2702765/1‑100 (MQ=255)
                                                                                 tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTCTCGGGTTATCGCTAAGGAATTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcgc                 >  1:2023693/1‑95 (MQ=255)
                                                                                    tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  1:2752813/1‑100 (MQ=255)
                                                                                    tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  2:2731267/1‑100 (MQ=255)
                                                                                    tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCGGACGTAAGTAATCGCGGAATGCGCGAACGAtt         <  2:3603003/100‑1 (MQ=255)
                                                                                     gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCTATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        <  1:896727/100‑1 (MQ=255)
                                                                                       aGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACg      <  1:3040012/100‑1 (MQ=255)
                                                                                        gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt     >  2:2636717/1‑100 (MQ=255)
                                                                                        gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt     >  2:1466052/1‑100 (MQ=255)
                                                                                          gATATACTGACGTGCGAGTTCGAGCACTTTGGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       <  1:728418/96‑1 (MQ=255)
                                                                                           atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:3116846/100‑1 (MQ=255)
                                                                                           atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  >  1:3583610/1‑100 (MQ=255)
                                                                                               |                                                                                               
CTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143546‑1143736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: