Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,755,639	G→A	100%	intergenic (‑498/‑59)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,639	0	G	A	100.0%	304.9 / ‑6.7	92	intergenic (‑498/‑59)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/49);  minor base T (1/0);  total (43/49)

CTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA  >  NC_000913/1755542‑1755734                                                                                                  |                                                                                                cTGAGACACAAGCACACATTCCTCTGCACGGTTATTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACa                                                                                               >  1:2492197/1‑100 (MQ=255)     gaCACAAGCACACATTCCTCTGCACGCTTTTTCTATGTTACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGCTTCGCTTTCCGACAGTgc                                                                                           >  1:3014795/1‑100 (MQ=255)     gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                           >  1:736970/1‑100 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAACGAGGCACCACAACTTTCGTAATACCGGATTCGCTTTCCGACAGttctc                                                                                         >  1:1455528/1‑96 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                         >  2:1157134/1‑100 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                         >  2:3284803/1‑100 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                         >  1:1015489/1‑100 (MQ=255)         caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                       <  2:2776830/100‑1 (MQ=255)          aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                      <  2:2180901/100‑1 (MQ=255)           aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                     <  2:3602637/100‑1 (MQ=255)           aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                     <  1:2521151/100‑1 (MQ=255)             cacacaTTCCTCTGCACGCTTTATCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                      >  1:2196577/1‑97 (MQ=255)               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                 <  2:83425/100‑1 (MQ=255)                acaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGc                                                                                <  1:1517279/100‑1 (MQ=255)                   ttCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCaa                                                                              >  2:1849393/1‑99 (MQ=255)                     ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                           >  1:1895067/1‑100 (MQ=255)                      ctctGCACGCTTTTTTTTTTTTTTTTATCCTTAAAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTTCGCCCAGAAATCaact                                                                            >  1:1153942/1‑96 (MQ=255)                      ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGGTTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGttt                                                                          >  2:1687677/1‑100 (MQ=255)                          gCACGCTTTTTTGATGTCATCTATCCTTAGAGCCGGGCACCATCACTTTCGTAATACCGGATTCGCTTTCAGTAAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                      >  1:1309271/1‑100 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACGACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                      <  1:2432703/100‑1 (MQ=255)                           cccGCTTTTTCGATGTCACCTATCCTTCGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                     <  1:2301001/98‑1 (MQ=255)                            actcTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                     <  2:2831647/96‑1 (MQ=255)                            aCGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCa                                                                    >  1:1624960/1‑100 (MQ=255)                             cGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCAt                                                                   <  1:1350485/100‑1 (MQ=255)                              gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATc                                                                  <  1:164245/100‑1 (MQ=255)                                tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                >  2:2828034/1‑100 (MQ=255)                                   ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                             >  2:2480261/1‑100 (MQ=255)                                   ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                             >  2:2087779/1‑100 (MQ=255)                                    tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc                                                            >  1:2516945/1‑100 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                           <  2:1383651/100‑1 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                           <  2:772622/100‑1 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                           <  2:2984955/100‑1 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                           <  2:3299629/100‑1 (MQ=255)                                      gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa                                                          <  2:79741/100‑1 (MQ=255)                                      gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa                                                          >  1:333859/1‑100 (MQ=255)                                       aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCGTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                         <  1:2102970/100‑1 (MQ=255)                                        tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                        <  1:3536238/100‑1 (MQ=255)                                        tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                        <  1:3021835/100‑1 (MQ=255)                                           cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                     >  1:591762/1‑100 (MQ=255)                                           cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAACAAGTTTCTCCCATCCTTCTCAACTTaa                                                     >  2:465353/1‑100 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                   <  1:2416861/100‑1 (MQ=255)                                               tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                 >  1:3101788/1‑100 (MQ=255)                                                  ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              <  1:1995775/100‑1 (MQ=255)                                                  ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              >  1:3606100/1‑100 (MQ=255)                                                   cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                             >  1:2724464/1‑100 (MQ=255)                                                      agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagact                                          >  1:3712403/1‑100 (MQ=255)                                                      agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagact                                          <  2:3548520/100‑1 (MQ=255)                                                        agCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGt                                        >  2:519044/1‑100 (MQ=255)                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCTTTCTCAACTTAAAGACTAAGACTGTCa                                      <  1:3628026/100‑1 (MQ=255)                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                      <  2:478435/100‑1 (MQ=255)                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                      <  1:488043/100‑1 (MQ=255)                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                      <  2:2002488/100‑1 (MQ=255)                                                           gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                     >  1:3637805/1‑100 (MQ=255)                                                            aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                    <  1:1513166/100‑1 (MQ=255)                                                              gCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaa                                  >  2:1215785/1‑100 (MQ=255)                                                               caccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaa                                 >  2:835137/1‑100 (MQ=255)                                                               caccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCGCAACTTAAAGACTAAGACTGTCATGaaa                                 >  1:2835440/1‑100 (MQ=255)                                                                accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                <  1:431584/100‑1 (MQ=255)                                                                 ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa                               >  2:1294887/1‑100 (MQ=255)                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                              >  1:3111951/1‑100 (MQ=255)                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                              >  2:3008747/1‑100 (MQ=255)                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                              >  1:1575694/1‑100 (MQ=255)                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                              <  2:1128328/100‑1 (MQ=255)                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            <  1:1511565/100‑1 (MQ=255)                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            >  2:3015037/1‑100 (MQ=255)                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTATCATGAAAAAGa                             >  2:2833187/1‑99 (MQ=255)                                                                    ccacTTTCATAATACCCGATTCGCCTTCCGACTGTGCGCCCAGAAAGCAAGTTGCTCCCAACCTTCTCAACTTAAAGACTAAGACTGTTATGAAAAAGAc                            <  1:2082878/100‑1 (MQ=255)                                                                     cacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            <  1:1511762/99‑1 (MQ=255)                                                                            gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                    >  1:2410983/1‑100 (MQ=255)                                                                            gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTGCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                    <  2:162146/100‑1 (MQ=255)                                                                             tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt                   >  2:3614547/1‑100 (MQ=255)                                                                               aTACCTCATTTGCTTTCCTACAGTGCGCCTAGAAAGCAAGTTTCTCCCACCCGTCTCAACTTAAATACTACCACTGTCATGAAAAAGACCAAAATTGttt                 <  1:3265436/100‑1 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:3419300/100‑1 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  1:980172/100‑1 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:2032751/100‑1 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:1084789/100‑1 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                <  2:1895074/100‑1 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                <  2:1097134/100‑1 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                >  1:3693559/1‑100 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                <  2:727033/100‑1 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGAAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                <  1:2065497/100‑1 (MQ=255)                                                                                 aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                >  2:3416056/1‑99 (MQ=255)                                                                                  ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa              <  2:3420687/100‑1 (MQ=255)                                                                                   cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc             <  2:262958/100‑1 (MQ=255)                                                                                   cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc             >  1:3683152/1‑100 (MQ=255)                                                                                    ggATTCGCTTACGGACAGAGCGCCCAGAAAGAAAGTTTCTACCATCCTTCTCGACTTAAAGAGTAAGAAAAAAAAAAAAAAGACCAAAATTGTTTGc               <  1:2377307/97‑1 (MQ=255)                                                                                       ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc         <  2:1481080/100‑1 (MQ=255)                                                                                          gCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGa      <  2:2937268/100‑1 (MQ=255)                                                                                           cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc     >  2:3548703/1‑100 (MQ=255)                                                                                           cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc     <  2:883659/100‑1 (MQ=255)                                                                                            tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc    <  1:1431126/100‑1 (MQ=255)                                                                                               ccGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGa  >  2:2982744/1‑99 (MQ=255)                                                                                                  |                                                                                                CTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA  >  NC_000913/1755542‑1755734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑