Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,472,522 C→T 26.7% A120A (GCG→GCA)  yfdN ← CPS‑53 (KpLE1) prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,472,5220CT26.7% 240.7 / 95.0 146A120A (GCG→GCA) yfdNCPS‑53 (KpLE1) prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (52/54);  new base T (20/19);  total (73/73)
Fisher's exact test for biased strand distribution p-value = 8.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 157 total aligned reads displayed.

TCACCTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCG  >  NC_000913/2472424‑2472621
                                                                                                  |                                                                                                   
tCACCTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATgccg                                                                                                    <  2:3614148/100‑1 (MQ=255)
tCACCTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATgccg                                                                                                    >  1:3396271/1‑100 (MQ=255)
 cACCTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATgccgc                                                                                                   <  2:1810137/100‑1 (MQ=255)
 cACCTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATgccgc                                                                                                   <  2:2714525/100‑1 (MQ=255)
   ccTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCa                                                                                                 >  1:2716247/1‑100 (MQ=255)
     tGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATc                                                                                               >  1:2915600/1‑100 (MQ=255)
      gATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATcg                                                                                              <  1:2370920/100‑2 (MQ=255)
       aTGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATcc                                                                                              <  2:356934/99‑1 (MQ=255)
       aTGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCca                                                                                             <  2:2724772/100‑1 (MQ=255)
         gCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCcaca                                                                                           <  2:2126038/100‑1 (MQ=255)
         gCAGCCCGCTCACTGCTATTCCGTAAACACCGTTCACGACCCGCCAGAAAACTGTTTCGTTCTGGCTGGGAGTGGCGTTGACGGAATGCCGCCATCcac                                                                                            >  1:2362436/1‑99 (MQ=255)
                gCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTgc                                                                                    <  1:1712934/100‑1 (MQ=255)
                 cgtACTGCTTTTCCGTAACCACGTGTCACGACGCGCCAGAAAATGGTTTCGTTCTGGGTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGttccn                                                                                   <  1:3028214/98‑4 (MQ=255)
                 cTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTgca                                                                                   <  1:1711733/100‑1 (MQ=255)
                  ttACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCGGGGACCGCCGCGATCTACACCGTTgcag                                                                                  <  1:2455059/100‑1 (MQ=255)
                  ttACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTgcag                                                                                  <  1:2771686/100‑1 (MQ=255)
                  ttACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTgcag                                                                                  <  1:3298120/100‑1 (MQ=255)
                  ttACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTgcag                                                                                  <  2:3642615/100‑1 (MQ=255)
                  ttACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTgcag                                                                                  <  2:2341869/100‑1 (MQ=255)
                     cTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCac                                                                               >  2:2724880/1‑100 (MQ=255)
                     cTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCac                                                                               >  2:585643/1‑100 (MQ=255)
                     cTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCac                                                                               >  1:988779/1‑100 (MQ=255)
                       gCTTTTCGGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCacga                                                                             >  1:1983828/1‑100 (MQ=255)
                       gCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCacga                                                                             >  2:1058837/1‑100 (MQ=255)
                       gCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTCCCATCCACACCGTTGCAGCacg                                                                              >  2:737007/1‑99 (MQ=255)
                       gCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCacga                                                                             >  2:1287372/1‑100 (MQ=255)
                        cTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCacgac                                                                            >  2:171207/1‑100 (MQ=255)
                          tttCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACgg                                                                          <  1:850636/100‑1 (MQ=255)
                          tttCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACgg                                                                          >  2:3375646/1‑100 (MQ=255)
                             ccGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGtat                                                                       >  1:1149380/1‑100 (MQ=255)
                             ccGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGtat                                                                       >  2:493514/1‑100 (MQ=255)
                              cGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGtata                                                                      >  1:2116228/1‑100 (MQ=255)
                               gTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATTCCGCCATCCACACCGTTGCAGCACGACGGTATaa                                                                     >  1:931400/1‑100 (MQ=255)
                                tAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAg                                                                    >  2:2033688/1‑100 (MQ=255)
                                  aaCACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGcc                                                                  <  1:2859805/100‑1 (MQ=255)
                                  aaCACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGcc                                                                  >  1:748232/1‑100 (MQ=255)
                                   acacCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGACTGCCGCCATACACACCGTTGCAGCACGACGGTATaa                                                                     >  1:1596831/1‑96 (MQ=255)
                                    cacCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGcccc                                                                >  2:3447572/1‑100 (MQ=255)
                                      ccGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTg                                                              >  2:2657594/1‑100 (MQ=255)
                                      ccGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTg                                                              >  2:23860/1‑100 (MQ=255)
                                            acgacgCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTcc                                                        <  2:496044/100‑1 (MQ=255)
                                             cgacgCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCa                                                       >  1:1042479/1‑100 (MQ=255)
                                               acgCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGt                                                     >  1:71275/1‑100 (MQ=255)
                                                cgcgCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGtt                                                    <  2:2926160/100‑1 (MQ=255)
                                                cgcgCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGtt                                                    >  2:3241972/1‑100 (MQ=255)
                                                   gcCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGttctt                                                 >  2:3297877/1‑100 (MQ=255)
                                                    ccAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGttcttc                                                <  2:3206386/100‑1 (MQ=255)
                                                    ccAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGttcttc                                                <  2:3400748/100‑1 (MQ=255)
                                                    ccAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGttcttc                                                >  2:3799492/1‑100 (MQ=255)
                                                     cAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTcc                                               >  1:2381339/1‑100 (MQ=255)
                                                      aGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCg                                              <  1:977593/100‑1 (MQ=255)
                                                      aGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCg                                              <  2:3800629/100‑1 (MQ=255)
                                                       gAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGc                                             >  2:1060807/1‑100 (MQ=255)
                                                        aaaaTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGcc                                            <  2:2302353/100‑1 (MQ=255)
                                                          aaTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTg                                          >  2:1890044/1‑100 (MQ=255)
                                                           aTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTgg                                         >  1:3804153/1‑100 (MQ=255)
                                                           aTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTgg                                         >  2:2510332/1‑100 (MQ=255)
                                                           aTTGTTTCGTTCTGGCGGGGAGTGGCGTTCTCGGAATGCTGCCAGCGACGCCGTTGCAGCACGACGGTAGGAGGCCCTGGACGCCGGGTCTTCCgtcgg                                          <  1:161538/99‑5 (MQ=255)
                                                            ttGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGc                                        <  2:1837484/100‑1 (MQ=255)
                                                             tGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCg                                       <  2:2536801/100‑1 (MQ=255)
                                                              gtctcgttcgtGCTGGGAGTGGCTTTCCCGGAATGCTCCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCgg                                      <  1:3510831/97‑1 (MQ=255)
                                                              gTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCgg                                      >  2:629259/1‑100 (MQ=255)
                                                               tttCGTTCTGGCTGGTAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCggg                                     <  2:3750781/100‑1 (MQ=255)
                                                               tttCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCggg                                     <  2:1132968/100‑1 (MQ=255)
                                                                 tCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTc                                   <  2:3647203/100‑1 (MQ=255)
                                                                  cGTTCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCa                                  <  2:1182570/100‑1 (MQ=255)
                                                                  cGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCa                                  <  1:1465584/100‑1 (MQ=255)
                                                                   gTTCTGGCTGGGCGTGGCTTTAACGGAATGCAGCCATCCACACCGTTGCAGCACGACGGTATAAGCCGCTGGACTCCAGTTCTTCCGACTGGCGGGACAg                                 >  1:3621597/1‑100 (MQ=255)
                                                                   gTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATTCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAg                                 >  2:217994/1‑100 (MQ=255)
                                                                    ttCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAgg                                <  2:1348686/100‑1 (MQ=255)
                                                                    ttCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAgg                                <  1:3044992/100‑1 (MQ=255)
                                                                     tCTGGCTGGGAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGc                               <  2:1520105/100‑1 (MQ=255)
                                                                     tCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGc                               >  1:2548229/1‑100 (MQ=255)
                                                                      ctggctggGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCGGGca                              >  1:1145746/1‑100 (MQ=255)
                                                                            gggAGTGGCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAAt                        >  2:1948296/1‑100 (MQ=255)
                                                                              gAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCa                      <  2:1685412/100‑1 (MQ=255)
                                                                              gAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCa                      <  2:2867855/100‑1 (MQ=255)
                                                                               aGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCAc                     >  2:2582184/1‑100 (MQ=255)
                                                                                gTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCAcc                    >  1:287698/1‑100 (MQ=255)
                                                                                 tGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCAccc                   <  1:1961576/100‑1 (MQ=255)
                                                                                   gCTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCcgc                 <  2:1567911/100‑1 (MQ=255)
                                                                                   gCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCcgc                 <  1:1354736/100‑1 (MQ=255)
                                                                                    cTTTCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCcgcg                >  2:299779/1‑100 (MQ=255)
                                                                                    cTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCcgcg                >  2:3065795/1‑100 (MQ=255)
                                                                                     tttCACGGAATGCTGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCgg               <  2:3766532/100‑1 (MQ=255)
                                                                                     tttCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCgg               >  2:1136083/1‑100 (MQ=255)
                                                                                      ttCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCggg              <  1:1672782/100‑1 (MQ=255)
                                                                                       tCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCCCAAAATCACCCGCgg               >  1:869212/1‑98 (MQ=255)
                                                                                        cACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTc            <  1:3196217/100‑1 (MQ=255)
                                                                                         aCGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCg           <  2:3788551/100‑1 (MQ=255)
                                                                                          cGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGt          <  1:1054556/100‑1 (MQ=255)
                                                                                           ggAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGtt         <  1:3332206/100‑1 (MQ=255)
                                                                                            gAATGCTGCCATCCACACCGTTGCAGCAGGGCGGTATAAGCCCCTCTTCTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGtt         <  1:1480456/99‑1 (MQ=255)
                                                                                             aaTGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCTCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAg       <  1:1349480/100‑1 (MQ=255)
                                                                                              aTGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGt      <  1:2312132/100‑1 (MQ=255)
                                                                                                gccgccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGc    <  1:493512/100‑1 (MQ=255)
                                                                                                gccgccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGc    >  1:539232/1‑100 (MQ=255)
                                                                                                 ccgccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGcc   <  2:294512/100‑1 (MQ=255)
                                                                                                  cgccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCg  <  1:337437/100‑1 (MQ=255)
                                                                                                  cgccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCg  >  2:2825784/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TCACCTGATGCAGCCCGCTTACTGCTTTTCCGTAAACACCGTTCACGACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCG  >  NC_000913/2472424‑2472621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑