Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,159 G→A 15.4% intergenic (+26/‑112) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1590GA15.4% 449.3 / 59.7 187intergenic (+26/‑112)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (67/89);  new base A (15/14);  total (82/105)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

Only 100 of 193 total aligned reads displayed.

TCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAT  >  NC_000913/2971060‑2971258
                                                                                                   |                                                                                                   
tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                                     >  2:295199/1‑100 (MQ=255)
 cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggn                                                                                                    <  1:2959291/100‑2 (MQ=255)
   aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                  >  2:3797608/1‑100 (MQ=255)
    cTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGt                                                                                                 <  2:1603805/100‑1 (MQ=255)
    cTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGt                                                                                                 <  2:931646/100‑1 (MQ=255)
     ttCGCGATGTTCTTCTCTATTGCGTTTGAAAGCCTCAACTTGATACGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGt                                                                                                 <  1:1217568/99‑1 (MQ=255)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  2:1496884/100‑1 (MQ=38)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  1:71902/100‑1 (MQ=38)
       cgcgAGGTTCTTCTCTATTGCGGTTGATAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCCGCAGGAGAGCGTac                                                                                               <  1:1780359/99‑1 (MQ=255)
         cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgga                                                                                            <  1:1381401/100‑4 (MQ=38)
          gATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacggag                                                                                           <  2:597679/100‑5 (MQ=37)
              ttcttcTCTATTGCAGTTGAAAGCCTCAAATTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacggagtatc                                                                                       <  1:1487505/100‑9 (MQ=25)
                 ttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtttt                                                                                    <  2:3764267/100‑1 (MQ=255)
                 ttctCTATGGCGGTTGAAGGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                      <  1:168554/98‑1 (MQ=255)
                  tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTa                                                                                   <  1:3391779/100‑1 (MQ=255)
                  tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTa                                                                                   <  1:1746305/100‑1 (MQ=255)
                   ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                                  <  2:2551006/100‑1 (MQ=255)
                    tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                                 <  2:3058056/100‑1 (MQ=255)
                    tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                                 >  2:2085546/1‑100 (MQ=255)
                    tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                                 <  2:2676447/100‑1 (MQ=255)
                        ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                             >  2:85385/1‑100 (MQ=255)
                        ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                             <  1:1005492/100‑1 (MQ=255)
                           cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                          >  2:3132206/1‑100 (MQ=255)
                             gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                                        <  1:1768365/100‑1 (MQ=255)
                               tGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                                      <  2:3472306/100‑1 (MQ=255)
                                gAAAGCCTAAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                     >  1:1666207/1‑100 (MQ=255)
                                 aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                                    >  2:580832/1‑100 (MQ=255)
                                   aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                                  >  1:697134/1‑100 (MQ=255)
                                   aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                                  <  2:134739/100‑1 (MQ=255)
                                    gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                 <  2:2521517/100‑1 (MQ=255)
                                    gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                 >  1:670243/1‑100 (MQ=255)
                                     ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGTGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                                      <  1:3737986/94‑1 (MQ=255)
                                       tCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTATGATTTTc                                                              <  1:1982495/100‑1 (MQ=255)
                                        cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                             >  2:1030613/1‑100 (MQ=255)
                                        cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                             <  1:1342490/100‑1 (MQ=255)
                                        cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                             <  2:976198/100‑1 (MQ=255)
                                         aaCTTGATTCGCAACAAAAAGAATCTGCTGTGATACTCCGTACGCTCTCCTGCGGTAGAGCGTACGCACTCCTTTTACTGTCACAGTTTAAGATTTTcat                                                            >  1:3181157/1‑98 (MQ=255)
                                          aCTTGATTCGCAACAAAAAGAATCCGCTCTGATACCCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                           <  1:863480/100‑1 (MQ=255)
                                               aTTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTc                                                      <  1:343156/100‑1 (MQ=255)
                                                ttCGCAACAAAAAGAATCCTCTCTCATACTCCGTACGCTCTCCTGCTGTAGTGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                     <  1:3121648/100‑1 (MQ=255)
                                                ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                     >  2:2807754/1‑100 (MQ=255)
                                                ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                     <  1:1432785/100‑1 (MQ=255)
                                                ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                     >  2:1173871/1‑100 (MQ=255)
                                                 tCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                                    >  2:1509081/1‑100 (MQ=255)
                                                 tCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                                    <  2:1004507/100‑1 (MQ=255)
                                                  cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                   <  2:451457/100‑1 (MQ=255)
                                                   gctacgaaaaGAATCAGCTCGGATACTCCGTACGCTCTCGTAAGGGGGGGCGTACTGCATACTTTTGCCATCGCAGTTTAAGATTTGCCtac                                                          >  1:3163765/7‑90 (MQ=255)
                                                   gCAACGACAAGAATCCGCTCTGATGCTCCTTCCGCTCTCTTGCTGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                                    <  1:3793136/98‑1 (MQ=255)
                                                   gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                  >  2:1686658/1‑100 (MQ=255)
                                                     aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                                >  2:1096323/1‑100 (MQ=255)
                                                      acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                               <  1:1850661/100‑1 (MQ=255)
                                                      acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                               <  2:3580970/100‑1 (MQ=255)
                                                       caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                              <  2:2198112/100‑1 (MQ=255)
                                                        aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatatat                                             >  1:1133107/1‑100 (MQ=255)
                                                         aaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                            >  1:306411/1‑100 (MQ=255)
                                                          aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATaa                                           >  1:1940327/1‑100 (MQ=255)
                                                           aaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAAc                                          <  2:1525368/100‑1 (MQ=255)
                                                              aaTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTc                                       >  1:909987/1‑100 (MQ=255)
                                                               aTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                                      <  2:3684332/100‑1 (MQ=255)
                                                               aTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCATACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                                      >  2:1289907/1‑100 (MQ=255)
                                                                  cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCAGtt                                   >  1:892628/1‑100 (MQ=255)
                                                                  cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                   <  1:1839514/100‑1 (MQ=255)
                                                                  cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                   <  2:2865210/100‑1 (MQ=255)
                                                                  cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                   >  1:2379727/1‑100 (MQ=255)
                                                                    ctctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTta                                 <  1:3313103/100‑1 (MQ=255)
                                                                    ctctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTta                                 >  2:1535379/1‑100 (MQ=255)
                                                                         aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAAc                            <  2:3577472/100‑1 (MQ=255)
                                                                         aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAAc                            <  1:2182400/100‑1 (MQ=255)
                                                                            gtgagTACGCCCTCCGGCACGAGAGCGTACTCACCCCGCTCACCATCAGCGTGGAATAACGTCCAGCTTTCAAGATATATAACGCCCATTTATAAACa                           >  1:1631749/5‑98 (MQ=255)
                                                                            cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                         <  1:3641977/100‑1 (MQ=255)
                                                                             tgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                        >  2:2421997/4‑100 (MQ=38)
                                                                              gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                       >  1:3695012/3‑100 (MQ=38)
                                                                              gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                       >  2:664539/3‑100 (MQ=38)
                                                                              ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCTCCCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCTGTTTATAAACCGAcg                       <  2:895108/100‑2 (MQ=255)
                                                                              ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                       <  2:1181918/100‑1 (MQ=255)
                                                                              ccGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGGTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                       <  1:1557950/100‑1 (MQ=255)
                                                                               cGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                      >  2:2454252/1‑100 (MQ=255)
                                                                               agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                      >  1:2532640/2‑100 (MQ=38)
                                                                                gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat                     <  2:2509807/100‑1 (MQ=255)
                                                                                gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat                     >  2:3336611/1‑100 (MQ=38)
                                                                                gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat                     >  2:3412755/1‑100 (MQ=38)
                                                                                   cgcttgccTTCGGTAGGGCGTACTCACTCCTTTTACCCTCCCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat                  <  1:1392931/94‑1 (MQ=255)
                                                                                     ctctCCTGCGGGAGGGGGTAATAACTATTTTTACCTTCACAGTTTAAGATTTTCCGGTTTTCAAGATATATAACGTCGATTTATAAATAGACtatta                   >  1:1862712/1‑97 (MQ=255)
                                                                                     ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa                >  1:1605429/1‑100 (MQ=255)
                                                                                     ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa                >  2:485850/1‑100 (MQ=255)
                                                                                       ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta              <  1:2815844/100‑1 (MQ=255)
                                                                                       ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta              <  2:1722434/100‑1 (MQ=255)
                                                                                        tcCTGCGGGATGACGTACTCATTCCTTTTACCTTCACAATTTAAGATTTTCCTGCTTTCAAGATATATAACATCGGTTTATAAACAGACTATTATCAta              <  1:3266467/99‑1 (MQ=255)
                                                                                        tcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtag             <  2:3082521/100‑1 (MQ=255)
                                                                                         ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg            >  1:2436664/1‑100 (MQ=255)
                                                                                         ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg            <  2:2098469/100‑1 (MQ=255)
                                                                                         ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg            <  2:1902382/100‑1 (MQ=255)
                                                                                          cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt           >  2:1233748/1‑100 (MQ=255)
                                                                                          cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt           >  2:3160458/1‑100 (MQ=255)
                                                                                           ngCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggta          >  2:1644680/2‑100 (MQ=255)
                                                                                            gCGGGAGGGCGTACTCACTCGTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag         >  1:2321117/1‑100 (MQ=255)
                                                                                             cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg        <  2:2039355/100‑1 (MQ=255)
                                                                                              gggAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATAGAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGc       <  1:2145522/100‑1 (MQ=255)
                                                                                                gAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCa     <  1:464366/100‑1 (MQ=255)
                                                                                                gAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGACa     <  2:469942/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAT  >  NC_000913/2971060‑2971258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: