Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,352,673 T→C 100% E118G (GAA→GGA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,6730TC99.1% 460.0 / ‑4.6 135E118G (GAA→GGA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (73/60);  total (74/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGA  >  NC_000913/3352576‑3352768
                                                                                                 |                                                                                               
gAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                               >  1:2742328/1‑100 (MQ=255)
gAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                               <  1:139053/100‑1 (MQ=255)
   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                            <  1:1056107/100‑1 (MQ=255)
   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                            >  2:1947694/1‑100 (MQ=255)
   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                            >  1:2041794/1‑100 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           <  2:316310/100‑1 (MQ=255)
    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                           >  2:3036643/1‑100 (MQ=255)
       aTTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTa                                                                                        <  2:2297371/100‑1 (MQ=255)
          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                     >  2:3505913/1‑100 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                  <  1:1254611/100‑1 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                  <  2:1344702/100‑1 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                 <  1:948677/100‑1 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                 <  2:2627675/100‑1 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                >  2:3690259/1‑100 (MQ=255)
                tctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCaa                                                                               >  2:3729195/1‑100 (MQ=255)
                 gttagcgaTCTTTGATTTCAATTTTCAGTAGGCCGAAAGTGTACTAAAATTATGCTTCCGCTTTTTAATGAGAGGAATGTCCCTGTTTTAGCTGGGGAat                                                                              >  1:2964538/5‑100 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                             >  2:355357/1‑100 (MQ=255)
                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                            <  1:270277/100‑1 (MQ=255)
                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCGGCTTCCGCTTTTTCACGAACAGCAATTCCCTGATTTAGCTGGGCAatat                                                                            <  1:1234273/100‑1 (MQ=255)
                    cgcgCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatt                                                                           <  1:1897837/100‑1 (MQ=255)
                    cgcgCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatt                                                                           >  1:3077095/1‑100 (MQ=255)
                     gcgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatta                                                                          >  1:1383725/1‑100 (MQ=255)
                      cgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtattat                                                                         >  2:765046/1‑100 (MQ=255)
                        ctctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCt                                                                       >  2:2202301/1‑100 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCCCGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                     >  2:1265451/1‑100 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                     <  2:2887472/100‑1 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                    >  2:2114136/1‑100 (MQ=255)
                             tGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTaa                                                                  >  1:979205/1‑100 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:635535/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATGTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:2440352/100‑1 (MQ=255)
                               aTTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACt                                                                >  1:164869/1‑100 (MQ=255)
                               aTTTCAACTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATACCCTGATTTAGCTGGGCAATATTATCTTTTAACt                                                                >  1:1990391/1‑100 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGCTTTAGCTTGGCAATATTATCTTTTAACt                                                                >  2:273641/1‑99 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:2644498/98‑1 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                               >  2:1611652/1‑100 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                               >  2:1870586/1‑100 (MQ=255)
                                 ttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGACCGGCAATTCCCTAATTTAGCTGGACAATATTATCATTTAACTGa                                                              >  2:1110970/1‑100 (MQ=255)
                                 ttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGa                                                              >  1:986546/1‑100 (MQ=255)
                                 ttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGa                                                              >  1:1030539/1‑100 (MQ=255)
                                 ttCAACTTTCAGTTTGCCGAAGGTTTCCTTCAGTTCTGCTTCCGTTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  1:1546917/97‑1 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                             >  2:677728/1‑100 (MQ=255)
                                     aTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTTTTTTTTTTTTTTTCCCTGAATTAGCTACGCAATATTTTCTTTTAACTGAAc                                                            >  1:93091/1‑98 (MQ=255)
                                     aTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGt                                                          >  2:670056/1‑100 (MQ=255)
                                        ttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGa                                                       >  2:3740215/1‑100 (MQ=255)
                                          cAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGa                                                       <  2:313302/98‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                    <  2:2482123/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCCCTTTTTCACGAACGGCAATTCCCTGAGTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAgg                                                     <  1:1998770/99‑1 (MQ=255)
                                            gTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCt                                                   <  2:1166712/100‑1 (MQ=255)
                                               ggCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACTGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaa                                                >  1:1286595/1‑100 (MQ=255)
                                                gCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaaa                                               >  1:2715663/1‑100 (MQ=255)
                                                gCCGAAGGCGTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGCTTTAGCTGGGCACTCTTCTCTTTTCACTGAACGTTGAGGCTCaaa                                               >  1:840300/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                              >  2:2098465/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                              >  2:287362/1‑100 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                              >  2:3350126/1‑100 (MQ=255)
                                                 cagAAGGTTTCCTGCAGTTCTGCTTCCGCTTATTCACGAACGTCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                              >  2:2560380/3‑100 (MQ=255)
                                                  cGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtn                                             <  1:2746642/100‑2 (MQ=255)
                                                  cGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtc                                             >  1:697053/1‑100 (MQ=255)
                                                  cGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtc                                             >  1:250304/1‑100 (MQ=255)
                                                     aGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcg                                          <  2:2356963/100‑1 (MQ=255)
                                                     aGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcg                                          <  1:3224499/100‑1 (MQ=255)
                                                      ggTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                         <  2:120799/100‑1 (MQ=255)
                                                       gTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcg                                          >  1:3412088/1‑98 (MQ=255)
                                                         ttCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcg                                      <  2:1472214/100‑1 (MQ=255)
                                                            cTGCATGTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCGGATTTAGCTGGGCAATATGATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                   >  1:173320/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGTAATTCCCTGATTTAGGTGGGCAATATTGTCTTTTAACTGAACGTGGAGGCTCAAAtcgcgctcgcgn                                   <  1:2262048/99‑2 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  <  1:2982191/100‑1 (MQ=255)
                                                              gCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAt                                 >  2:440026/1‑100 (MQ=255)
                                                               cAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtc                                <  1:460768/100‑1 (MQ=255)
                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTACCTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtctc                              >  2:2667322/1‑100 (MQ=255)
                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtctc                              >  2:3448593/1‑100 (MQ=255)
                                                                  ttCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcc                             <  2:3076936/100‑1 (MQ=255)
                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcct                            >  2:3284096/1‑100 (MQ=255)
                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCGGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcc                             >  2:3529295/1‑99 (MQ=255)
                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                          <  2:1034694/100‑1 (MQ=255)
                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                          <  1:3236925/100‑1 (MQ=255)
                                                                       cTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAg                        <  2:2060824/100‑1 (MQ=255)
                                                                        ttCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGt                       >  1:2164503/1‑100 (MQ=255)
                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAgag                      >  1:2060817/1‑98 (MQ=255)
                                                                          ccGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttt                     >  2:175064/1‑100 (MQ=255)
                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                   <  1:3592249/100‑1 (MQ=255)
                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                   <  1:3161129/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  1:377498/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  2:2692512/100‑1 (MQ=255)
                                                                               ttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCa                <  2:3792268/100‑1 (MQ=255)
                                                                               tttaCACGACGGGCGATTCCCTGATGTAGCTGGGCAATATTATCTGTTAACTGATCGTTGAGGCTCAAATCGCCCTCGCGCATCTCCTCCAGttttt                   <  1:3461359/97‑1 (MQ=255)
                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc              <  2:949055/100‑1 (MQ=255)
                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc              >  2:2268909/1‑100 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                   >  1:161605/1‑94 (MQ=255)
                                                                                   cACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAg            <  2:3065005/100‑1 (MQ=255)
                                                                                    aCGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAg            >  1:357144/1‑99 (MQ=255)
                                                                                    aCGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGc           <  1:102438/100‑1 (MQ=255)
                                                                                     ccaaCGGCAATTTCTTGAGTTAGCAGGACAATATTATCTTTTAACTGAACATTTAGGTTCAAATTGCTCCCGCGCATCAACTCTAGCTTTTGCACCAGc           >  1:223122/3‑99 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          >  2:3641075/1‑100 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          <  1:230267/100‑1 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          >  2:320331/1‑100 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          <  2:2344180/100‑1 (MQ=255)
                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc          <  1:3208413/100‑1 (MQ=255)
                                                                                         cGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGa      >  1:637989/1‑100 (MQ=255)
                                                                                            cAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAg   <  2:1383730/100‑1 (MQ=255)
                                                                                            cAATTCCCTGATTTAGCGGGGCAATATTATCTTTTAACTGAAAGTTGAGGGACAAATCGGGCTAGCGCATCGCCTCAAGTTTTTGCACCAGCCGTGACa    >  1:3163583/1‑99 (MQ=255)
                                                                                                 |                                                                                               
GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGA  >  NC_000913/3352576‑3352768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: