Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,018,260 (A)8→7 coding (315/444 nt) fliJ → flagellar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,018,2530A.98.4% 548.3 / 2.6 124coding (308/444 nt)fliJflagellar protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (53/68);  total (54/70)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348
                                                                                                   |                                                                                               
agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  1:1098250/1‑100 (MQ=255)
agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  1:12018/1‑100 (MQ=255)
    gTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaa                                                                                             >  2:3611266/1‑100 (MQ=255)
    gTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaa                                                                                             >  2:2128851/1‑100 (MQ=255)
    gTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaa                                                                                             >  1:3953062/1‑100 (MQ=255)
     tttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaa                                                                                            >  1:1884745/1‑100 (MQ=255)
       tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           <  2:3204836/99‑1 (MQ=255)
         tCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaa                                                                                        >  1:3572636/1‑97 (MQ=255)
          ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       <  2:231524/100‑5 (MQ=255)
           cAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTGATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACg                                                                                     <  2:2939276/100‑1 (MQ=255)
           cAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACg                                                                                     >  1:90337/1‑100 (MQ=255)
           cAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACg                                                                                     >  1:3709617/1‑100 (MQ=255)
             gACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGtt                                                                                   >  2:2916539/1‑100 (MQ=255)
             gACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGtt                                                                                   <  1:2178835/100‑1 (MQ=255)
              aCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGttt                                                                                  >  1:233100/1‑100 (MQ=255)
               cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACCGTTGGCGAGAAAAAAACAACGTTTg                                                                                 >  2:3860907/1‑100 (MQ=255)
               cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTg                                                                                 >  2:1321972/1‑100 (MQ=255)
               cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTg                                                                                 <  1:2654828/100‑1 (MQ=255)
                gCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAACACAACGTTTGc                                                                                <  2:2096735/100‑1 (MQ=255)
                gCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGc                                                                                <  2:780823/100‑1 (MQ=255)
                 cttgAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               >  2:3913213/4‑100 (MQ=255)
                 cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               <  1:2448343/100‑1 (MQ=255)
                 cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               <  2:366489/100‑1 (MQ=255)
                 cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               >  2:1357062/1‑100 (MQ=255)
                  tagAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGACAAGAAAAAAACAACGTTTGTAg                                                                              <  2:4046420/98‑1 (MQ=255)
                    gAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGc                                                                            >  1:240786/1‑100 (MQ=255)
                    gAAAAAGACATTACTCATATTCGCAAGCAACCTAATCAGTGTACGCAGAAAGTTGACATTGCCCTGAACATTTAGAAAAAAAAAAACAACGttt                                                                                  <  2:2539141/94‑1 (MQ=255)
                     aaaaaGCCATTACTCAGCATCGCCAGCAATTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGGTGACGAGAAAAAAACAACGTTTGCAGGcc                                                                           <  2:3709576/100‑1 (MQ=255)
                     aaaaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGcc                                                                           <  1:1567499/100‑1 (MQ=255)
                       aaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTg                                                                         <  1:3995175/100‑1 (MQ=255)
                       aaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTg                                                                         <  2:295835/100‑1 (MQ=255)
                        aaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTgg                                                                        >  1:1932812/1‑100 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGc                                                                       <  1:3388992/100‑1 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGc                                                                       <  2:2220734/100‑1 (MQ=255)
                          gCCATTCCTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCATGCCTGGCa                                                                      <  2:1129070/100‑1 (MQ=255)
                          gCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCa                                                                      <  1:776514/100‑1 (MQ=255)
                          gCCATTACTCAGCATAGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCa                                                                      <  2:1129324/100‑1 (MQ=255)
                                  tCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              <  2:1933495/100‑1 (MQ=255)
                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             >  2:322363/1‑100 (MQ=255)
                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             <  1:688667/100‑1 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGTAGACACTGCAg                                                            <  1:2355616/100‑1 (MQ=255)
                                     gCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAgg                                                           >  2:285780/1‑100 (MQ=255)
                                     gCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAgg                                                           <  2:1035989/100‑1 (MQ=255)
                                     gCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAgg                                                           >  1:4175214/1‑100 (MQ=255)
                                       aTCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGaa                                                         >  1:2668935/1‑100 (MQ=255)
                                        tCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAAc                                                        <  1:3903226/100‑1 (MQ=255)
                                          gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACgg                                                      >  2:2277314/1‑100 (MQ=255)
                                          gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACgg                                                      >  1:2063605/1‑100 (MQ=255)
                                            cAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCa                                                    <  1:1095833/100‑1 (MQ=255)
                                             ngCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCaa                                                   >  1:3787660/2‑100 (MQ=255)
                                                aaCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATcc                                                <  1:3923529/100‑1 (MQ=255)
                                                 aCTTCATCAGGGGATGCAGAGAGTTGACATTGCGCTGAACAGTTGGCCGGAAAAAAACAACGTTTGCAGGACTGGCAGACACTGCACGCACGGCat                                                   >  2:2843235/1‑95 (MQ=255)
                                                 aCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCa                                               <  2:2592777/100‑1 (MQ=255)
                                                 aCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCa                                               >  1:1961039/1‑100 (MQ=255)
                                                  cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              >  1:2038125/1‑100 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  1:3280123/1‑100 (MQ=255)
                                                     aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggc                                           <  1:416426/100‑1 (MQ=255)
                                                     aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggc                                           >  2:713033/1‑100 (MQ=255)
                                                         aGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCa                                       >  1:3174194/1‑100 (MQ=255)
                                                          gTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCAc                                      <  2:1307063/100‑1 (MQ=255)
                                                           tGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCCGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACt                                     <  2:2231599/100‑1 (MQ=255)
                                                            ggACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTg                                    >  2:3444466/1‑100 (MQ=255)
                                                             gACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGc                                   <  1:3611305/100‑1 (MQ=255)
                                                               cGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCtt                                 >  2:1583961/1‑100 (MQ=255)
                                                                gCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTg                                >  1:690354/1‑100 (MQ=255)
                                                                 naGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               >  2:2010967/2‑100 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAACAACAACGTTTGCAGGCCTGTCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:2280644/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               >  1:2731533/1‑100 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:4111112/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:713372/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  1:3969311/100‑1 (MQ=255)
                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCGGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  1:2916571/100‑1 (MQ=255)
                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  1:2918494/100‑1 (MQ=255)
                                                                  aGAAAGTTGAAATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  1:1266408/99‑1 (MQ=255)
                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGTGGCACTGCTTGCAg                             >  1:339655/1‑100 (MQ=255)
                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             <  1:1259843/100‑1 (MQ=255)
                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             <  1:375812/100‑1 (MQ=255)
                                                                   gAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGCCAATCCACGGCGGCACTGCTTGCAg                             >  2:2478295/1‑100 (MQ=255)
                                                                     aaGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaa                           <  1:2128871/100‑1 (MQ=255)
                                                                     aaGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaa                           >  1:126472/1‑100 (MQ=255)
                                                                     aaGTTGACATTGCCCTGAACAATTGGCGAAAAAAAATCAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaa                           <  2:1173983/100‑1 (MQ=255)
                                                                      aGTTGACATTGCCCTGAACAGTCGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaa                          >  2:2432175/1‑100 (MQ=255)
                                                                        ttGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAAc                        <  2:923303/100‑1 (MQ=255)
                                                                               tGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCg                 >  2:3020364/1‑100 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                <  1:900466/100‑1 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                >  2:897285/1‑100 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                <  1:3590064/100‑1 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCCCGa                >  1:1785324/1‑100 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAAGAACGGCAATCCACAGCGGCACTGCTTGCAGAAAACCGCCTCGa                >  2:1877265/1‑100 (MQ=255)
                                                                                 cccTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               >  1:2496566/1‑100 (MQ=255)
                                                                                 cccTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               >  2:3709964/1‑100 (MQ=255)
                                                                                  ccagAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGTAGACACTGCAGGAACGGCTATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCg                 <  2:1520474/94‑1 (MQ=255)
                                                                                    tGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAg            <  1:285785/100‑1 (MQ=255)
                                                                                     gAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGa           >  2:523731/1‑100 (MQ=255)
                                                                                      aaCAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaa          <  2:348817/100‑1 (MQ=255)
                                                                                        tccatGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        <  1:4059348/96‑1 (MQ=255)
                                                                                        cAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        <  1:2630600/100‑1 (MQ=255)
                                                                                            tGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgan    <  2:3787618/100‑2 (MQ=255)
                                                                                             ggCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   <  2:2155524/100‑1 (MQ=255)
                                                                                              gCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  1:1434731/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: