Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,470,410 (T)7→6 coding (1280/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4040T.98.1% 1153.6 / 13.9 260coding (1274/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base . (111/143);  total (115/145)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

AATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTA  >  NC_000913/2470305‑2470499
                                                                                                   |                                                                                               
    ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttt                                                                                             <  1:2254250/100‑1 (MQ=255)
     tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttt                                                                                            >  1:2654210/1‑100 (MQ=255)
      aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGCTCGACCTTTGCTTATACATAACAGTCGtttttta                                                                                           <  2:1356273/100‑2 (MQ=255)
        gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                         >  1:1950156/1‑97 (MQ=255)
         tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                        >  1:3008229/1‑96 (MQ=255)
          gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAAttt                                                                                      >  1:3577704/1‑100 (MQ=255)
          gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAAttt                                                                                      >  2:1454094/1‑100 (MQ=255)
           tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAAttt                                                                                      <  2:2265797/99‑1 (MQ=255)
           tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTta                                                                                     <  1:1276065/100‑1 (MQ=255)
             aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTtata                                                                                   <  2:1316756/100‑1 (MQ=255)
             aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTtata                                                                                   >  2:2169370/1‑100 (MQ=255)
              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTta                                                                                     <  2:1156644/97‑1 (MQ=255)
              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaa                                                                                  <  1:2336254/100‑1 (MQ=255)
                tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaaa                                                                                <  1:1103622/100‑1 (MQ=255)
                tttCGGGGAAGGATATAGAGATATAAGCAAGCATTTATTTGGAATGTATTTTTCTTTTTTCTTTTGCTTATACATAACAGTCGTTTTTTAATTTATaa                                                                                  >  2:2424139/1‑98 (MQ=255)
                 ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaat                                                                               >  1:441321/1‑100 (MQ=255)
                 ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaat                                                                               <  1:3488996/100‑1 (MQ=255)
                    ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTACTTTATAAaata                                                                              >  2:3727140/1‑98 (MQ=255)
                    ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaataat                                                                            >  1:1833383/1‑100 (MQ=255)
                     gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAAtt                                                                           <  1:1430846/100‑1 (MQ=255)
                     gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAAtt                                                                           >  1:74359/1‑100 (MQ=255)
                       gAAGGATATAGAGATTTAAGCAAGCATTTATTTGAAATGTATTTTTCGTTCGACCTTTGATTATACATAACAGTCGTTTTTTAATTTATAAaataat                                                                            <  2:3530979/97‑1 (MQ=255)
                        aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaa                                                                        >  2:2226761/1‑100 (MQ=255)
                        aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCa                                                                         <  2:3037334/99‑1 (MQ=255)
                         aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTCTTCTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAATTAATTCaaa                                                                       >  2:3903986/1‑100 (MQ=255)
                         aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaaa                                                                       <  1:1015469/100‑1 (MQ=255)
                          ngATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTACGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                      >  2:2321267/2‑100 (MQ=255)
                          ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                      <  1:712704/100‑1 (MQ=255)
                           gATATAGATATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                      <  2:1710578/99‑1 (MQ=255)
                           gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGa                                                                     >  1:94934/1‑100 (MQ=255)
                            atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                      <  2:57045/98‑1 (MQ=255)
                            atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGaa                                                                    >  1:2593124/1‑100 (MQ=255)
                             tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTTTTTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGaa                                                                    >  2:547173/1‑99 (MQ=255)
                             tataGAGATTTAAGCAAGCATTTATTTGAAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGaaa                                                                   <  2:3243957/100‑1 (MQ=255)
                               taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                 >  1:2752637/1‑100 (MQ=255)
                               taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                 <  2:2064247/100‑1 (MQ=255)
                                agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGATCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                <  2:4085012/100‑1 (MQ=255)
                                 gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                               >  1:1203243/1‑100 (MQ=255)
                                 gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                               >  2:319716/1‑100 (MQ=255)
                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                              <  2:722848/100‑1 (MQ=255)
                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                              <  1:4013675/100‑1 (MQ=255)
                                   gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                             >  2:185678/1‑100 (MQ=255)
                                     tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                           >  1:4041173/1‑100 (MQ=255)
                                      ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                          >  1:2471972/1‑100 (MQ=255)
                                       tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAt                                                         >  2:2501416/1‑100 (MQ=255)
                                        aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                        <  1:601524/100‑1 (MQ=255)
                                         agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                       <  2:358361/100‑1 (MQ=255)
                                          gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                      >  2:4077605/1‑100 (MQ=255)
                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAGTTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                     <  1:2937407/100‑1 (MQ=255)
                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                     >  1:4134194/1‑100 (MQ=255)
                                             agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                   >  1:2671909/1‑100 (MQ=255)
                                              gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                  <  1:1699356/100‑1 (MQ=255)
                                                atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                >  2:3118009/1‑100 (MQ=255)
                                                 tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                               <  1:3586821/100‑1 (MQ=255)
                                                    ntttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            >  2:307717/2‑100 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  2:3499617/100‑1 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  2:3353587/100‑1 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  2:2028574/100‑1 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  1:3045171/100‑1 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  1:3117334/100‑1 (MQ=255)
                                                     tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                           <  2:4126645/100‑1 (MQ=255)
                                                      ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCan                                          <  1:842101/100‑2 (MQ=255)
                                                      ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                          <  2:1203234/100‑1 (MQ=255)
                                                      ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                          <  1:17730/100‑1 (MQ=255)
                                                      ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                          >  1:2889178/1‑100 (MQ=255)
                                                       tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                          <  2:827641/99‑1 (MQ=255)
                                                       tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                         <  1:1501606/100‑1 (MQ=255)
                                                       tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                         >  1:3881178/1‑100 (MQ=255)
                                                          aaTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                      <  1:2149990/100‑1 (MQ=255)
                                                            tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                    <  2:3295872/100‑1 (MQ=255)
                                                               aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                 >  2:910367/1‑100 (MQ=255)
                                                               aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                 <  1:2394386/100‑1 (MQ=255)
                                                                 ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAt                               <  2:3254557/100‑1 (MQ=255)
                                                                  tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                              <  2:765828/100‑1 (MQ=255)
                                                                    tcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                            >  1:1872852/1‑100 (MQ=255)
                                                                     cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                           <  1:1601027/100‑1 (MQ=255)
                                                                     cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                           >  2:2598681/1‑100 (MQ=255)
                                                                     cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                           <  1:4072105/100‑1 (MQ=255)
                                                                      gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGCTGTAAAGCACTAAGTTTAAATTgcgc                          <  2:3772600/100‑1 (MQ=255)
                                                                      gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                          <  2:3825582/100‑1 (MQ=255)
                                                                       ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                         <  1:3274202/100‑1 (MQ=255)
                                                                       ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                         <  1:3719477/100‑1 (MQ=255)
                                                                       ttcgACCTTTGCTTATACATAACAGTCTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                         <  2:3695411/100‑1 (MQ=255)
                                                                         cgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                       <  2:2896602/100‑1 (MQ=255)
                                                                         cgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                       <  2:1525828/100‑1 (MQ=255)
                                                                          gACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                      <  1:2287567/100‑1 (MQ=255)
                                                                           aCCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCaa                     >  1:544205/1‑100 (MQ=255)
                                                                            ccTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                    >  1:3638589/1‑100 (MQ=255)
                                                                            ccTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                    >  1:1299565/1‑100 (MQ=255)
                                                                               ttGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                 <  2:1005538/100‑1 (MQ=255)
                                                                               ttGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                 <  2:1081990/100‑1 (MQ=255)
                                                                                 gCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg               >  2:2713130/1‑100 (MQ=255)
                                                                                  cTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc              <  2:3457068/100‑1 (MQ=255)
                                                                                  cTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc              <  1:3793531/100‑1 (MQ=255)
                                                                                       aCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca         >  2:3848895/1‑100 (MQ=255)
                                                                                        cttAACAGTCGTTGTTTAATTTATAAAATAATTCAAAGAAATGAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac        >  1:2830299/3‑100 (MQ=255)
                                                                                        cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGCTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGAGcac        >  1:2056039/1‑100 (MQ=255)
                                                                                        cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac        >  2:2564850/1‑100 (MQ=255)
                                                                                         aTAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcaca       <  1:2543517/100‑1 (MQ=255)
                                                                                              aGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta  <  1:2194070/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTA  >  NC_000913/2470305‑2470499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: