Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,645,886 (C)5→4 coding (1440/2313 nt) pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,645,8820C.100.0% 564.7 / NA 122coding (1444/2313 nt)pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (67/55);  total (67/55)

Only 100 of 138 total aligned reads displayed.

TGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTG  >  NC_000913/2645783‑2645977
                                                                                                   |                                                                                               
 ggTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTGCATCAGCCATAATCCGACGAAGGATCCACgccgcc                                                                                                >  2:2694279/1‑100 (MQ=255)
   tGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGcccc                                                                                              >  1:3503547/1‑100 (MQ=255)
    gCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccg                                                                                             >  2:2460123/1‑99 (MQ=255)
     cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccga                                                                                            <  1:1346774/100‑3 (MQ=255)
     cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccga                                                                                            >  1:83513/1‑98 (MQ=255)
      ccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgac                                                                                           >  2:1442715/1‑97 (MQ=255)
      acGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgac                                                                                           >  2:1620283/2‑97 (MQ=255)
       cGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCCGCCATAATCCGGCGAATGATCCACGCCGccccgacg                                                                                          >  2:4104185/1‑96 (MQ=255)
       cGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgacg                                                                                          <  1:4060604/100‑5 (MQ=255)
       cGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgacg                                                                                          >  2:3578545/1‑96 (MQ=255)
       cGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgacg                                                                                          >  1:4163647/1‑96 (MQ=255)
        gTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCCGCAAGGGTTGCGCTTCATCACCCATACTCCGGCGAATGATCAACGCCGccccgac                                                                                           >  2:3361348/1‑95 (MQ=255)
           tgccATGCCAGTGGGGCGACGCTCGGCAACTCACTATCCTGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACa                                                                                      <  2:484252/97‑1 (MQ=255)
            tCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACAtt                                                                                    >  2:2714829/1‑100 (MQ=255)
            tCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACAtt                                                                                    >  2:3289223/1‑100 (MQ=255)
            tCCATGCCAGTGGGGCGACGCGCGGCAAGACACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAACCCGGCGAATGATCCACGCCG‑CCCCgacgac                                                                                       >  2:3445126/1‑97 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTa                                                                                   >  1:1895167/1‑100 (MQ=255)
              cATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCTGGCGAATGATCCACGCCG‑CCCCGACGACATTaa                                                                                  <  2:1337066/100‑1 (MQ=255)
               aTGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGGTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTaaa                                                                                 >  2:991928/1‑100 (MQ=255)
                tGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAg                                                                                <  1:2581276/100‑1 (MQ=255)
                 gCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAgg                                                                               >  1:2432652/1‑100 (MQ=255)
                 gCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAgg                                                                               >  1:1240295/1‑100 (MQ=255)
                 gCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAgg                                                                               <  1:1860002/100‑1 (MQ=255)
                  ccAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGACG‑CCCCGACGACATTAAAGGc                                                                              <  2:289756/100‑1 (MQ=255)
                    aGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCg                                                                            >  1:418814/1‑100 (MQ=255)
                     gTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGt                                                                           <  1:437110/100‑1 (MQ=255)
                     gTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGt                                                                           >  2:41746/1‑100 (MQ=255)
                     gTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGt                                                                           <  2:74007/100‑1 (MQ=255)
                      tGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACCACATTAAAGGCCGtt                                                                          >  1:4117387/1‑100 (MQ=255)
                        gggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCa                                                                        >  1:849305/1‑100 (MQ=255)
                           cGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagc                                                                     <  1:2416311/100‑1 (MQ=255)
                           cGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagc                                                                     <  2:3936814/100‑1 (MQ=255)
                           cGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagc                                                                     <  1:3753585/100‑1 (MQ=255)
                              cgcgcgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagcagc                                                                  >  1:501342/1‑100 (MQ=255)
                                cgcgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagcagc                                                                  <  2:1371037/98‑1 (MQ=255)
                                cgcgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCgg                                                                >  1:2700746/1‑100 (MQ=255)
                                  cgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCggc                                                               >  2:2532345/1‑98 (MQ=255)
                                  cgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGAt                                                              <  2:376147/100‑1 (MQ=255)
                                    gCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCTCGCCG‑CCCCGTCGACATTAAAGGCCGTTCAAGCAGCGGATCg                                                            <  1:2826394/100‑1 (MQ=255)
                                    gCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCg                                                            <  1:3130534/100‑1 (MQ=255)
                                     cAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATc                                                             >  2:1148153/1‑98 (MQ=255)
                                     cAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGt                                                           >  1:2673241/1‑100 (MQ=255)
                                       aGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACCTTAAAGGCCGTTCAAGCAGCGGATCGTCa                                                         >  1:3226293/1‑100 (MQ=255)
                                         gCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAgg                                                       >  1:2571858/1‑100 (MQ=255)
                                              aTCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTa                                                  <  1:41746/100‑1 (MQ=255)
                                                ccGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTCAg                                                >  2:1015795/1‑100 (MQ=255)
                                                ccGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAg                                                >  2:2352803/1‑100 (MQ=255)
                                                ccGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAg                                                >  1:3903938/1‑100 (MQ=255)
                                                ccGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAg                                                >  1:2939881/1‑100 (MQ=255)
                                                 cGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgc                                               <  2:436469/100‑1 (MQ=255)
                                                  ggCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgcg                                              <  1:1301601/100‑1 (MQ=255)
                                                   gCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgcgc                                             >  2:3252046/1‑100 (MQ=255)
                                                      aGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAAt                                          <  2:1913887/100‑1 (MQ=255)
                                                       gggTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAAtt                                         >  2:2393537/1‑100 (MQ=255)
                                                       gggTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAAtt                                         >  1:1678542/1‑100 (MQ=255)
                                                        ggTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAAttt                                        <  1:736627/100‑1 (MQ=255)
                                                          ttGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgc                                      <  2:382731/100‑1 (MQ=255)
                                                           tGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcc                                     <  2:1488217/100‑1 (MQ=255)
                                                            gcgcTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcct                                    >  2:1919528/1‑100 (MQ=255)
                                                            gcgcTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcct                                    <  2:2972146/100‑1 (MQ=255)
                                                             cgcTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcctg                                   >  1:215338/1‑100 (MQ=255)
                                                               cTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcctgcc                                 >  2:27337/1‑100 (MQ=255)
                                                                ttCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTgcctgcct                                <  2:3434816/100‑1 (MQ=255)
                                                                   atcaGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGc                             <  1:1750739/100‑1 (MQ=255)
                                                                   atcaGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGc                             >  2:3381155/1‑100 (MQ=255)
                                                                   atcaGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGc                             >  1:2773163/1‑100 (MQ=255)
                                                                    tcaGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGcc                            >  1:3079967/1‑100 (MQ=255)
                                                                     caGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCg                           >  2:3534136/1‑100 (MQ=255)
                                                                      aGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGcc                            <  2:985490/98‑1 (MQ=255)
                                                                      aGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGt                          >  1:1036715/1‑100 (MQ=255)
                                                                      aGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGt                          >  1:1343162/1‑100 (MQ=255)
                                                                        ccATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTgg                        >  1:1682409/1‑100 (MQ=255)
                                                                          aTAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      >  1:503983/1‑100 (MQ=255)
                                                                          aTAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      >  1:154178/1‑100 (MQ=255)
                                                                          aTAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      >  1:3990590/1‑100 (MQ=255)
                                                                          aTAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      <  1:2028350/100‑1 (MQ=255)
                                                                            aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    <  1:3224833/100‑1 (MQ=255)
                                                                            aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    <  2:813068/100‑1 (MQ=255)
                                                                            aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    <  1:1132488/100‑1 (MQ=255)
                                                                            aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    <  2:1456717/100‑1 (MQ=255)
                                                                            aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    >  1:454170/1‑100 (MQ=255)
                                                                            aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    >  2:3191914/1‑100 (MQ=255)
                                                                             aTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGc                   <  2:83513/100‑1 (MQ=255)
                                                                             aTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGc                   >  1:3559562/1‑100 (MQ=255)
                                                                              tCCGGCGAATGATCCAGGCCG‑CCGCGACGACATTAAAGGCCGGTCAAGCAGCGGATCGGCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      <  2:3914171/96‑1 (MQ=255)
                                                                              tCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCa                  <  1:2887722/100‑1 (MQ=255)
                                                                                      aTGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAATGCGGt          >  1:328400/1‑100 (MQ=255)
                                                                                      aTGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGt          <  1:457192/100‑1 (MQ=255)
                                                                                       tGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGta         >  1:1098982/1‑100 (MQ=255)
                                                                                       tGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGta         <  2:836590/100‑1 (MQ=255)
                                                                                       tGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGta         >  2:2370165/1‑100 (MQ=255)
                                                                                        gATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGtat        >  1:3485490/1‑100 (MQ=255)
                                                                                         aTCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGtata       >  2:3494006/1‑100 (MQ=255)
                                                                                          tCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATAc      >  2:3610243/1‑100 (MQ=255)
                                                                                            cACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGc    <  1:39939/100‑1 (MQ=255)
                                                                                            cACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGc    >  2:102329/1‑100 (MQ=255)
                                                                                             aCGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCt   <  1:3824239/100‑1 (MQ=255)
                                                                                             aCGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCt   >  2:781549/1‑100 (MQ=255)
                                                                                              cgccg‑cCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTg  >  1:1421139/1‑100 (MQ=255)
                                                                                              cgccg‑cCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGAAATTTGCCTGCCTTGCCGTGGAGAGCAAACGCGGAATACGCTg  >  1:2436337/1‑100 (MQ=255)
                                                                                                   |                                                                                               
TGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTG  >  NC_000913/2645783‑2645977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑