Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,639,879 (T)8→7 intergenic (‑159/‑232) uspB ← / → uspA universal stress (ethanol tolerance) protein B/universal stress global response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,639,8720T.95.8% 808.2 / 25.8 192intergenic (‑152/‑239)uspB/uspAuniversal stress (ethanol tolerance) protein B/universal stress global response regulator
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base . (91/93);  total (97/95)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 216 total aligned reads displayed.

CGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTC  >  NC_000913/3639773‑3639967
                                                                                                   |                                                                                               
cGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgt                                                                                                 >  1:1992657/1‑100 (MQ=255)
     cGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttt                                                                                            >  2:1766742/1‑100 (MQ=255)
     cGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttt                                                                                            <  2:2378291/100‑1 (MQ=255)
      gCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGttttttt                                                                                           >  2:862997/1‑100 (MQ=255)
       cTGCCAATCTGTTAACACCAGGTCTTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttc                                                                                          <  1:949274/100‑2 (MQ=255)
       cTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttc                                                                                          >  1:1775210/1‑99 (MQ=255)
        tGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcc                                                                                         <  1:4147952/100‑3 (MQ=255)
        tGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcc                                                                                         <  1:2218221/100‑3 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        >  2:4127229/1‑97 (MQ=255)
           cAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGa                                                                                     <  1:3463783/100‑1 (MQ=255)
            aaTCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGaa                                                                                    <  1:3412908/100‑1 (MQ=255)
             aTCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAg                                                                                   <  1:764010/100‑1 (MQ=255)
             aTCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAg                                                                                   >  2:22630/1‑100 (MQ=255)
              tCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAgg                                                                                  <  1:3100179/100‑1 (MQ=255)
                aGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGtt                                                                                >  2:3016021/1‑100 (MQ=255)
                aGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGtt                                                                                >  1:408544/1‑100 (MQ=255)
                 gTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTa                                                                               >  2:1090678/1‑100 (MQ=255)
                 gTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTa                                                                               >  1:3513311/1‑100 (MQ=255)
                   tAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTaa                                                                              >  1:1155051/1‑100 (MQ=255)
                   tAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAc                                                                             >  2:1441362/1‑100 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAAc                                                                             <  1:1351847/100‑1 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            <  2:2030110/100‑1 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            >  2:1257742/1‑100 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            <  1:465572/100‑1 (MQ=255)
                       acCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACt                                                                         >  1:2623062/1‑100 (MQ=255)
                         cAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTAt                                                                       >  1:552286/1‑100 (MQ=255)
                            gTCCTGGAGAAAACGCTTTTTTGGTGACCAACATACGAGCGGCTCTATAGATAGTTTAGGAGATCAGGTTTTTTTTTTCCAGAAGGTTAACCACTATCaa                                                                    <  2:1566732/100‑1 (MQ=255)
                             tCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAat                                                                   <  2:3706603/100‑1 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCACCATACGAGCGGCCCCCTCGACAGTGCAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatc                                                                  <  1:3915090/100‑2 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAata                                                                  <  1:1823107/100‑1 (MQ=255)
                               cTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatat                                                                 >  1:2814249/1‑100 (MQ=255)
                               cTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatat                                                                 >  1:10055/1‑100 (MQ=255)
                                tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatata                                                                <  1:1533727/100‑1 (MQ=255)
                                tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatata                                                                >  2:2324192/1‑100 (MQ=255)
                                tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatata                                                                >  1:4014818/1‑100 (MQ=255)
                                tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatata                                                                <  1:3385193/100‑1 (MQ=255)
                                 ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCAGATGTTTAACCACTATCAatatat                                                               >  2:1705090/1‑100 (MQ=255)
                                 ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatatat                                                               <  1:2324212/100‑1 (MQ=255)
                                  gagaAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCTAGAAGGTTAACCACTATCAATATAtt                                                              <  1:2551629/100‑1 (MQ=255)
                                   agaAACCGCTTTTGTGGTGACCAACATACGATCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTc                                                             <  2:3352718/100‑1 (MQ=255)
                                     aaaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCAt                                                           <  2:1644201/100‑1 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATg                                                          <  1:3678292/100‑1 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATg                                                          >  2:441175/1‑100 (MQ=255)
                                      aaCAGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATg                                                          <  2:381188/100‑1 (MQ=255)
                                       aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGt                                                         <  2:1329970/100‑1 (MQ=255)
                                        ccGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTTATATGTTTAACCACTATCAATATATTCATGTc                                                        >  2:361459/1‑100 (MQ=255)
                                        ccGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCATAAGGTTAACCACTATCAATATATTCATGt                                                         >  2:337708/1‑99 (MQ=255)
                                        ccGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTc                                                        >  1:3570286/1‑100 (MQ=255)
                                        ccGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTc                                                        >  2:2125207/1‑100 (MQ=255)
                                           cTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGaa                                                     >  1:6658/1‑100 (MQ=255)
                                            ttttGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGaaa                                                    >  2:2797644/1‑100 (MQ=255)
                                             tttGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGaaaa                                                   <  1:147517/100‑1 (MQ=255)
                                              ttGTGGTGACCAACATACGAGCGGCTCTATAGATCGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTGATGTCGAAAAt                                                  <  2:2200705/100‑1 (MQ=255)
                                                gtggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAAttt                                                >  2:2154421/1‑100 (MQ=255)
                                                 tggtgACCAACATACGAGCGTCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTg                                               <  2:593355/100‑1 (MQ=255)
                                                 tggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTg                                               <  1:1723840/100‑1 (MQ=255)
                                                 tggtgACCAACATACGAGCGGCTCTAAAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTg                                               >  1:2538337/1‑100 (MQ=255)
                                                  ggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGt                                              >  2:1371159/1‑100 (MQ=255)
                                                  ggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGt                                              <  2:846738/100‑1 (MQ=255)
                                                  ggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGt                                              >  2:209097/1‑100 (MQ=255)
                                                   gtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtt                                             <  1:3785279/100‑1 (MQ=255)
                                                   gtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtt                                             >  2:3296227/1‑100 (MQ=255)
                                                     gACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTa                                           >  2:81961/1‑100 (MQ=255)
                                                      aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATGCATGTCGAAAATTTGTTTAt                                          <  2:2551013/100‑1 (MQ=255)
                                                       ccAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATc                                         >  2:1138072/1‑100 (MQ=255)
                                                         aaCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTa                                       <  2:3173529/100‑1 (MQ=255)
                                                         aaCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTa                                       >  2:46977/1‑100 (MQ=255)
                                                           cATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAAc                                     <  1:1094155/100‑1 (MQ=255)
                                                           cATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAAc                                     <  2:3740212/100‑1 (MQ=255)
                                                            ttACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAGTTTGTTTATCTAACg                                    <  2:1170220/99‑1 (MQ=255)
                                                            aTACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACg                                    >  1:2692048/1‑100 (MQ=255)
                                                            aTACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACg                                    >  1:981797/1‑100 (MQ=255)
                                                             tACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGa                                   >  2:1543203/1‑100 (MQ=255)
                                                               cGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGt                                 >  1:1283855/1‑100 (MQ=255)
                                                               cGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGt                                 >  1:1913321/1‑100 (MQ=255)
                                                                 aGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTa                                <  2:3520867/99‑1 (MQ=255)
                                                                  gCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAg                              >  2:4176198/1‑100 (MQ=255)
                                                                         tataGATAGTGTAGGAGATCAGGTTGTTTTTTTCTATATTGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg                       >  1:1203075/1‑100 (MQ=255)
                                                                         tataGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg                       <  1:244799/100‑1 (MQ=255)
                                                                          atagatagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCgg                      >  1:85185/1‑100 (MQ=255)
                                                                            agatagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAt                    >  2:1063498/1‑100 (MQ=255)
                                                                             gatagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                   <  2:3838282/100‑1 (MQ=255)
                                                                               tagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGa                 >  1:214982/1‑100 (MQ=255)
                                                                                agTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGAc                >  1:602774/1‑100 (MQ=255)
                                                                                 gtgtAGGAGATCAGGTTGTTTTTTTTTTTTTTTTTAACCACTATCAATATATTCATGTTGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTg                  >  2:3501185/1‑97 (MQ=255)
                                                                                 gtgtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACg               >  2:2915627/1‑100 (MQ=255)
                                                                                 gtgtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACg               <  2:10055/100‑1 (MQ=255)
                                                                                  tgtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACgg              >  2:3053130/1‑100 (MQ=255)
                                                                                   gtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGa             <  1:3253164/100‑1 (MQ=255)
                                                                                   gtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGa             >  1:3659218/1‑100 (MQ=255)
                                                                                   gtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGa             >  2:1456941/1‑100 (MQ=255)
                                                                                    tcggAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            <  2:2529885/98‑1 (MQ=255)
                                                                                    tAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            <  2:1929594/100‑1 (MQ=255)
                                                                                     aGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGatc           <  1:2483749/100‑1 (MQ=255)
                                                                                     aGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGatc           <  2:1962244/100‑1 (MQ=255)
                                                                                       gagaTCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGatcat         <  1:1441373/100‑1 (MQ=255)
                                                                                             aGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt   <  2:2814217/100‑1 (MQ=255)
                                                                                             aGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt   >  2:2362730/1‑100 (MQ=255)
                                                                                              ggTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCggg    <  1:2982962/98‑1 (MQ=255)
                                                                                              ggTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTc  <  2:39818/100‑1 (MQ=255)
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CGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTC  >  NC_000913/3639773‑3639967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: