Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,436,256 G→A A350A (GCG→GCA)  rsmB → 16S rRNA m(5)C967 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,436,2560GA98.7% 541.0 / ‑3.0 159A350A (GCG→GCA) rsmB16S rRNA m(5)C967 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (76/81);  total (77/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 159 total aligned reads displayed.

CATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAA  >  NC_000913/3436160‑3436351
                                                                                                |                                                                                               
cATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACaa                                                                                              >  1:2946614/1‑100 (MQ=255)
   tttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTg                                                                                           <  1:1505448/100‑1 (MQ=255)
    ttattaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGc                                                                                          <  1:1093415/100‑1 (MQ=255)
    ttattaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGc                                                                                          <  1:369524/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                        <  2:970282/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                        <  2:79109/100‑1 (MQ=255)
      attaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCACACAATTGCAg                                                                                        <  1:2570060/100‑1 (MQ=255)
       ttaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGt                                                                                       >  1:2491726/1‑100 (MQ=255)
        taGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTc                                                                                      >  2:2377407/1‑100 (MQ=255)
         aGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCt                                                                                     >  2:2261884/1‑100 (MQ=255)
          gATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTg                                                                                    <  2:546926/100‑1 (MQ=255)
           aTGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGa                                                                                   >  1:2972979/1‑100 (MQ=255)
           aTGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGa                                                                                   <  1:1001304/100‑1 (MQ=255)
            tGCGCCTTGTTCATCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGaa                                                                                  <  2:1555658/100‑1 (MQ=255)
            tGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTgn                                                                                   <  2:1888607/99‑2 (MQ=255)
               gcCTTGTTCAGCAACCGGGGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAAtt                                                                               >  2:166678/1‑100 (MQ=255)
                ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtc                                                                              <  2:3995592/100‑1 (MQ=255)
                 cTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                             <  1:450290/100‑1 (MQ=255)
                 cTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                             >  1:2499669/1‑100 (MQ=255)
                 cTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                             <  2:3220650/100‑1 (MQ=255)
                  ttGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtctc                                                                            <  1:3554022/100‑1 (MQ=255)
                   tGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCg                                                                           <  2:932398/100‑1 (MQ=255)
                    gTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGa                                                                          >  1:1743769/1‑100 (MQ=255)
                    gTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGa                                                                          >  2:359111/1‑100 (MQ=255)
                     ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGAc                                                                         >  1:236152/1‑100 (MQ=255)
                     ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGAc                                                                         <  1:609196/100‑1 (MQ=255)
                     ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGAc                                                                         <  1:3343316/100‑1 (MQ=255)
                      tCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACg                                                                        >  1:795604/1‑100 (MQ=255)
                      tCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACg                                                                        >  1:1845734/1‑100 (MQ=255)
                      tCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACg                                                                        >  1:2692547/1‑100 (MQ=255)
                       cAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGc                                                                       >  1:1622236/1‑100 (MQ=255)
                        aGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGc                                                                       <  1:353593/99‑1 (MQ=255)
                            aCCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCAttt                                                                  <  1:774568/100‑1 (MQ=255)
                             ccGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTg                                                                 <  1:902258/100‑1 (MQ=255)
                              cGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTgg                                                                <  2:2610568/100‑1 (MQ=255)
                               ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                               <  2:3386927/100‑1 (MQ=255)
                                gtgtGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGcc                                                              >  1:2118492/1‑100 (MQ=255)
                                 tgtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                               >  2:2539792/1‑98 (MQ=255)
                                 tgtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCg                                                             <  2:2898649/100‑1 (MQ=255)
                                  gtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGc                                                            <  2:798456/100‑1 (MQ=255)
                                  gtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGc                                                            >  2:655405/1‑100 (MQ=255)
                                   tgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCa                                                           >  2:3118934/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  2:14058/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  1:876477/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  2:512742/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                          >  1:317396/1‑100 (MQ=255)
                                    gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCACAt                                                          >  1:4013708/1‑100 (MQ=255)
                                     aTTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAtt                                                         >  1:3979883/1‑100 (MQ=255)
                                      ttCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAttt                                                        >  1:1302950/1‑100 (MQ=255)
                                      ttCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAttt                                                        >  1:3452101/1‑100 (MQ=255)
                                         gtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaa                                                     >  1:578469/1‑100 (MQ=255)
                                         gtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaa                                                     <  2:3322116/100‑1 (MQ=255)
                                          tcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCCGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaaa                                                    >  2:1443634/1‑100 (MQ=255)
                                          tcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaaa                                                    <  1:451836/100‑1 (MQ=255)
                                            gCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAAc                                                  <  1:3175485/100‑1 (MQ=255)
                                              cATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCg                                                >  2:3117488/1‑100 (MQ=255)
                                              cATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCg                                                >  2:392563/1‑100 (MQ=255)
                                              cATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCg                                                >  2:1609145/1‑100 (MQ=255)
                                              cATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCg                                                >  2:1608875/1‑100 (MQ=255)
                                               aTCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCgg                                               >  1:102001/1‑100 (MQ=255)
                                                tCCAGATATTAAATGGTTACGTCGTGATCGCGATACCCCTGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaa                                                      <  2:244814/92‑1 (MQ=255)
                                                tCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGt                                              >  1:3660758/1‑100 (MQ=255)
                                                 ccAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTg                                             >  2:551852/1‑100 (MQ=255)
                                                 ccAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTg                                             <  1:1867790/100‑1 (MQ=255)
                                                    gATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGaa                                          <  2:1516865/100‑1 (MQ=255)
                                                     ctatTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAAc                                         <  1:1279125/99‑1 (MQ=255)
                                                      tatTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAAct                                        >  1:1094148/1‑100 (MQ=255)
                                                       atTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAAcag                                       >  2:1279114/1‑98 (MQ=255)
                                                          aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTgg                                    <  2:1222999/100‑1 (MQ=255)
                                                          aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTgg                                    <  1:2642036/100‑1 (MQ=255)
                                                            aTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTc                                  <  2:1289334/100‑1 (MQ=255)
                                                            aTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTc                                  <  2:3385387/100‑1 (MQ=255)
                                                               gTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                               >  1:1094783/1‑100 (MQ=255)
                                                               gTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                               <  2:3476909/100‑1 (MQ=255)
                                                               gTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                               <  2:301785/100‑1 (MQ=255)
                                                               gTTACGTCGCGATCGCGATATCCCGGAACTCACACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                               <  2:4084805/100‑1 (MQ=255)
                                                                ttACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGGCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                               <  1:2451419/99‑1 (MQ=255)
                                                                  aCGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGcc                            >  1:1289458/1‑100 (MQ=255)
                                                                     tctcgatcgcgatATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                         <  2:1313168/97‑1 (MQ=255)
                                                                     tcgcgatcgcgaTATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                         >  1:2479754/1‑100 (MQ=255)
                                                                     tcgcgatcgcgaTATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                         <  1:3692078/100‑1 (MQ=255)
                                                                      ngcgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCt                        >  1:116449/2‑100 (MQ=255)
                                                                        cgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGt                      >  1:1989710/1‑100 (MQ=255)
                                                                         gatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGtt                     >  2:1082488/1‑100 (MQ=255)
                                                                            cgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCgg                  <  2:3056422/100‑1 (MQ=255)
                                                                            cgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCgg                  <  1:3118967/100‑1 (MQ=255)
                                                                            cgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCgg                  >  1:2393684/1‑100 (MQ=255)
                                                                               gatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCAACCTGTTCGgtgn               <  2:1017951/100‑2 (MQ=255)
                                                                                atatCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGtt              <  2:2491701/100‑1 (MQ=255)
                                                                                   tCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTAcc           <  2:795600/100‑1 (MQ=255)
                                                                                     ccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCgg         <  1:3555178/100‑1 (MQ=255)
                                                                                      cGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGa        <  2:8793/100‑1 (MQ=255)
                                                                                        gAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAag      <  1:144846/100‑1 (MQ=255)
                                                                                        gAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAag      <  2:1714297/100‑1 (MQ=255)
                                                                                         aaCTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAaga     <  1:683740/100‑1 (MQ=255)
                                                                                         aaCTCGCACAATTGCAGTCTGAAAGTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagn     <  2:604802/100‑2 (MQ=255)
                                                                                          aCTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagag    <  2:2692515/100‑1 (MQ=255)
                                                                                          aCTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagag    <  1:2789521/100‑1 (MQ=255)
                                                                                           cTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAgaga   >  1:762843/1‑100 (MQ=255)
                                                                                            tCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGaa  <  1:3048660/100‑1 (MQ=255)
                                                                                                |                                                                                               
CATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAA  >  NC_000913/3436160‑3436351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑