Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,553 (T)9→8 100% intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5450T.92.9% 494.3 / 30.8 126intergenic (+71/‑160)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (8/1);  new base . (63/54);  total (71/55)
Fisher's exact test for biased strand distribution p-value = 7.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 144 total aligned reads displayed.

TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107446‑107640
                                                                                                   |                                                                                               
tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGt                                                                                                 >  1:439508/1‑100 (MQ=255)
 cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtt                                                                                                >  2:739978/1‑100 (MQ=255)
   aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttt                                                                                              >  2:1185400/1‑100 (MQ=255)
    aGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttt                                                                                             >  1:399769/1‑100 (MQ=255)
    aGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttt                                                                                             >  1:1864100/1‑100 (MQ=255)
      cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                           <  1:1001449/100‑1 (MQ=255)
      cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                           <  1:1001422/100‑1 (MQ=255)
      cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                           <  2:322947/100‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCCGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  2:2406255/100‑1 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGGCGTATAAAATTCGACTGGCAAATCTGGCACTCTCGCCGGCCAGGTGAACCAGTCGttttttttg                                                                                         <  2:1905077/100‑2 (MQ=255)
         cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttga                                                                                        >  1:2046272/1‑98 (MQ=255)
         cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttga                                                                                        <  2:2975599/100‑3 (MQ=255)
         cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttga                                                                                        <  1:1244043/100‑3 (MQ=255)
           tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaa                                                                                       >  1:2770328/1‑96 (MQ=255)
            cAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAtt                                                                                    >  1:2223832/1‑100 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                                 >  1:459938/1‑100 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                                 <  1:2109543/100‑1 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                                 <  2:2251190/100‑1 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                                 <  1:1846434/100‑1 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                                 <  2:439502/100‑1 (MQ=255)
                tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtat                                                                                <  2:150493/100‑1 (MQ=255)
                      ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTATCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGc                                                                          <  2:448697/100‑1 (MQ=255)
                        cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCta                                                                        >  2:1436733/1‑100 (MQ=255)
                         aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGATTTTTTTGAATTTTATAAGAGCtat                                                                       >  1:137821/1‑100 (MQ=255)
                          tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCtata                                                                      >  2:2892472/1‑100 (MQ=255)
                           aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATaaaaacgata                                                                      >  1:2475562/1‑92 (MQ=255)
                            actaCATTTATACTGTCGTATAAAATTCGACTGGCTAATCTGGCACTTTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                    <  2:1288578/97‑1 (MQ=255)
                            aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                    >  1:3922163/1‑100 (MQ=255)
                             cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaaa                                                                   >  2:1359519/1‑100 (MQ=255)
                              gACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaaaa                                                                  <  2:268383/100‑1 (MQ=255)
                                cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                <  2:546662/100‑1 (MQ=255)
                                  tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCATTTTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                              <  1:1393646/100‑1 (MQ=255)
                                      taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCg                                                          <  2:3588901/100‑1 (MQ=255)
                                        cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                        >  1:2597223/1‑100 (MQ=255)
                                        cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                        >  2:1790452/1‑100 (MQ=255)
                                        cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                        >  1:2793975/1‑100 (MQ=255)
                                        cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                        >  1:1174630/1‑100 (MQ=255)
                                          gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      >  1:952625/1‑100 (MQ=255)
                                          gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      >  1:3911137/1‑100 (MQ=255)
                                             gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   >  1:945833/1‑100 (MQ=255)
                                               ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTTTTAGCTATAAAAAACGGTGCGAATTCTGtt                                                 >  2:2493575/1‑100 (MQ=255)
                                               ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   >  1:2180468/1‑98 (MQ=255)
                                                taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTAAGACCAATAAAAAAATCTACGAACGc                                                     >  2:526639/1‑95 (MQ=255)
                                                 aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt                                               >  1:3393721/1‑100 (MQ=255)
                                                 aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt                                               <  1:1832018/100‑1 (MQ=255)
                                                  aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                              >  1:2254344/1‑100 (MQ=255)
                                                    aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTAAGAGCTATAAAAAAAGGTGCGAACTCTGTTTTCt                                             >  2:464965/1‑99 (MQ=255)
                                                    aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                            >  1:2676681/1‑100 (MQ=255)
                                                     ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                           <  2:3771157/100‑1 (MQ=255)
                                                      tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAAAAAAAAAAAACGGTGCGAACGCTGTTTTCTTaa                                          <  2:1522286/100‑1 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                         >  2:3917686/1‑100 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAGCTATAAAAAACGATGCAAACGCTGTTATCTTaa                                          >  1:504719/1‑99 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                        <  1:2589499/100‑1 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                        <  2:1825609/100‑1 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCACCGGCCAGGTGAACCAGTCGCTTTTTTTGAATTTTATAACAGCTATAAAAAACGGACCGAACGCTGTTTTCTTaa                                          >  2:3080339/1‑98 (MQ=255)
                                                         tcTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                        <  1:2715215/98‑1 (MQ=255)
                                                         aCTGTCAAATATGTCACTCTCTCCGGTCGGGTGATCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                         <  1:1711562/98‑1 (MQ=255)
                                                         aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCa                                       >  2:794588/1‑100 (MQ=255)
                                                         aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAAAGGTGCGAACGCTGTTTTCTTAAGCa                                       >  2:16660/1‑100 (MQ=255)
                                                          cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      <  1:896627/100‑1 (MQ=255)
                                                           tgccAATTCTGGCTCTTTCTCCGGCCAGTTAAACCAGTCTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                         <  2:2465883/93‑1 (MQ=255)
                                                           tGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                     >  1:1565159/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  2:1736719/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAACTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  1:3043185/1‑100 (MQ=255)
                                                             gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt                                   >  2:3219673/1‑100 (MQ=255)
                                                              cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                  >  2:2848151/1‑100 (MQ=255)
                                                              cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                  >  1:96025/1‑100 (MQ=255)
                                                              cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑ATTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                                 >  2:2079807/1‑100 (MQ=255)
                                                                 aTCTGGCACTCTCTCCGGCCAGGTGAACCTGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                               <  1:638274/100‑1 (MQ=255)
                                                                 aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAACTATAAAAAAAGGTACGAACGCTGTTTTCTTAAGCACTTTTCCg                               >  1:904867/1‑100 (MQ=255)
                                                                  tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                              <  2:730320/100‑1 (MQ=255)
                                                                   cTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTTAAGAGCTATAAAAAACGGTCCGAACGCTGTTTTTTTAAGCACTTTTcc                                >  2:3605756/1‑98 (MQ=255)
                                                                   cTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAAAAGTACAAACACTGTTTTCTTAAGCACTTTTCCGca                             >  1:1453494/1‑100 (MQ=255)
                                                                    tGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                            >  1:2481462/1‑100 (MQ=255)
                                                                       cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcacatc                         >  2:3740544/1‑98 (MQ=255)
                                                                       cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                         >  1:1982065/1‑100 (MQ=255)
                                                                        aCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                        <  2:1569622/100‑1 (MQ=255)
                                                                         ctctctCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                        <  1:2147885/99‑1 (MQ=255)
                                                                         ctctctCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                        <  1:1359534/99‑1 (MQ=255)
                                                                          tctctcTGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTGATAAGATCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTa                      <  1:3626596/100‑1 (MQ=255)
                                                                          tctctcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTa                      <  1:2983064/100‑1 (MQ=255)
                                                                          tctctcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTa                      <  1:2983047/100‑1 (MQ=255)
                                                                              tcCGGCCAGGTGAACCAGTCGTTTTTTTTGTATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                  >  1:3357295/1‑100 (MQ=255)
                                                                              tcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                  <  2:1489201/100‑1 (MQ=255)
                                                                               ccGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTc                 >  2:2568234/1‑100 (MQ=255)
                                                                                cGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa                <  2:3354291/100‑1 (MQ=255)
                                                                                  gCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt              <  2:1636983/100‑1 (MQ=255)
                                                                                    cAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg            <  1:3917725/100‑1 (MQ=255)
                                                                                    cAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCAGCACAACTTATCTTCATTCg            >  2:1857655/1‑100 (MQ=255)
                                                                                      ggTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg          >  1:1265993/1‑100 (MQ=255)
                                                                                      ggTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCAGCACAACTTATCTTCATTCGTg          >  1:3664954/1‑100 (MQ=255)
                                                                                       gTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc         <  1:1300604/100‑1 (MQ=255)
                                                                                         gAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtg       <  1:2591406/100‑1 (MQ=255)
                                                                                          aaCCAGTCGTTTTTTTTGAATATTATAAGAAATATAAAAAACGGTAAGAAAGCTGTTTTCTTAAGCACTTTTTCGCCCAACTTATCTTCATTAGTGCt        >  2:3833350/1‑98 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     >  1:1615997/1‑100 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     <  2:2676657/100‑1 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     <  2:1488825/100‑1 (MQ=255)
                                                                                            ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg    <  2:2609336/100‑1 (MQ=255)
                                                                                            ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg    <  1:3616882/100‑1 (MQ=255)
                                                                                               gTCGTTTTTTTTTAATTTAATAAGAGCAAAAAAAAACAGTGCGAACGCTATTTTCTTAAGCACTTTTCCGAAAAACTTATCTTCATTCGTACtg       >  2:814221/1‑94 (MQ=255)
                                                                                                   |                                                                                               
TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107446‑107640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: