BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,379,266:1	(C)_7→8	100%	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	92.2%	423.4 / 32.8	141	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (4/7); new base C (65/64); total (69/72)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑c                                                                                                  >  1:2134326/1‑100 (MQ=255)

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGACCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑ttt                                                                                                      >  2:572176/1‑99 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc                                                                                                 >  2:136861/1‑100 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc                                                                                                 >  2:3752667/1‑100 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGACGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc                                                                                                 >  2:3405704/1‑100 (MQ=255)

  cacaGAGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc                                                                                                 <  2:3039165/100‑1 (MQ=255)

     aGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc‑c‑cc                                                                                            <  2:1622804/100‑1 (MQ=255)

       cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc‑c‑cc‑cc                                                                                         <  1:3642259/100‑1 (MQ=255)

        gtagtaAAAGAGACCGCCAGCGTCTGGTTCTTGACGTTCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc‑c‑cc‑ccc                                                                                        <  1:3529433/100‑2 (MQ=255)

        gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGATTTTTCTC‑‑G‑TTT‑‑‑cc‑c‑cc‑ccc                                                                                        <  2:1067815/100‑2 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc‑c‑cc‑cccgcc                                                                                     >  2:2727908/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑cc‑c‑cc‑cccgcc                                                                                     >  2:1814867/1‑96 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGATAAATTTTCTC‑‑A‑TTT‑‑‑CC‑CACC‑CCACCAg                                                                                    >  1:2573873/1‑100 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGACGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAg                                                                                    >  1:3317355/1‑100 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCTACGCCCATTGTTGGTAATGTCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAgcgc                                                                                 <  2:2594393/100‑1 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAgcgc                                                                                 <  2:2259325/100‑1 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGcc                                                                                <  2:2824981/100‑1 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGcc                                                                                >  2:3872875/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCaa                                                                              >  1:2209853/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGCTGAATTTTCTC‑‑G‑TTTCC‑CC‑C‑CC‑CCGCCAGCGCCa                                                                               >  2:2261154/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTTAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAAcc                                                                            >  2:2336460/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGACAGCGCCAAc                                                                             >  2:767482/1‑99 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCAt                                                                          <  1:3581374/100‑1 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCAt                                                                          >  2:2770058/1‑100 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGTTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGtt                                                                      <  1:2799301/100‑1 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTAt                                                                   <  1:1863538/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATc                                                                  >  1:3484012/1‑100 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGTTAATGGCTGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATcc                                                                 <  1:654290/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGTCGATGGTTGATTTATAACGTCGGTGAATGTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATcc                                                                 <  1:425879/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATcc                                                                 <  2:1357082/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATcc                                                                 <  2:1084156/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCa                                                                >  1:990871/1‑100 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCAtt                                                              >  2:3757436/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑CCAC‑CC‑CCGCCAGCGCCAACAATAGTTTATCCAttt                                                             >  1:927446/1‑99 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           >  2:1006509/1‑100 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           >  1:1258442/1‑100 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTAATTCCTAACGTCGGTAAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:422496/99‑1 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:1531484/1‑100 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                        <  2:498031/99‑1 (MQ=255)

                                           gCCCATTGTTGCTAATGTCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCg                                                      <  2:463213/100‑1 (MQ=255)

                                             ccgttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTCTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACAATAGTTTATCCATTTACGACCggg                                                    >  2:103285/4‑100 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                    <  1:1428887/100‑1 (MQ=255)

                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  <  2:3811945/100‑1 (MQ=255)

                                                ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  <  1:2838111/99‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               >  1:933341/1‑97 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:2826540/100‑1 (MQ=255)

                                                      cTAATGGCCGCTGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACTATTGTTTATCCATTTGCGCCCGGGTTTCATCGt                                           <  2:2580634/100‑1 (MQ=255)

                                                      cTAATGGCCGATGGTTGCTTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCCACCATAGTTTATCAATTTGCGCCCGCGTTTCATCGt                                           >  2:3168594/1‑100 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                           >  1:2381664/1‑100 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTACCCATTTGCGCCCGGGTTTCATc                                             >  1:3846036/1‑98 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          >  1:1692970/1‑100 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          <  1:2581746/100‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                         <  2:3600446/100‑1 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCCCCCGCGCCAACCATAGTTTACCCATTTGCGCCCGGGTTTCATCGTGCAg                                       >  2:899679/1‑100 (MQ=255)

                                                           ggCCTAAGGGTGATTTCTTAAGTCGGTTAATTTTTTC‑‑C‑TTTCCCCC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                        <  2:1476259/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  1:2959944/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  1:447464/1‑100 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   >  1:2029934/1‑100 (MQ=255)

                                                                aTGGTTGATTTCTAACGTCGGTTAATTTTTTTTTT‑TTT‑‑‑TT‑C‑TT‑TCTCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  1:1054134/100‑1 (MQ=255)

                                                                aTGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                 <  2:239699/100‑1 (MQ=255)

                                                                 tGGTTGATTTCTAACGTCGGTGAAATTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCTCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                >  2:2650899/1‑100 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTC‑‑T‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAAACATAGTTTATCCATGTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               <  1:2472368/100‑1 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGAGCCCGGGTTTCATCGTGAAGTGGCGaaa                               >  1:394159/1‑100 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCGATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                            >  2:2640470/1‑100 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑CCAC‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                           >  1:3723740/1‑100 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          <  2:3483973/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  1:1006520/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGAGCCAACCATAGCTTATCCATTTGCGCCCGGGTTTCCTCCTGAAGTGACGAAAGCcaca                         >  2:2517385/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTGCTC‑‑G‑TTA‑‑‑CC‑T‑CC‑CCGCCCGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          <  2:1976300/98‑1 (MQ=255)

                                                                         ttttAACGTCTGTAATTTTTTTC‑‑T‑TTT‑‑‑CC‑CTCC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        <  1:1079478/97‑1 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        >  1:3798220/1‑100 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCATAAAGTGGCGAAAGCcaca                         >  1:921902/1‑99 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                       <  2:2454709/100‑1 (MQ=255)

                                                                              aCGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                   <  2:415888/100‑1 (MQ=255)

                                                                               cGTCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCACCCGAGTTTAACCGTAACGTGGCGAAACACACCGATacgc                  >  1:3672210/1‑100 (MQ=255)

                                                                                 tCGGTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 <  1:3752706/99‑1 (MQ=255)

                                                                                  cGGTGAATTTTCTC‑‑T‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAATCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  1:1536832/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTC‑‑G‑TTTCC‑CC‑C‑CC‑CCGCCAGCTCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  2:2746593/100‑1 (MQ=255)

                                                                                    gTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCATCCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:1581481/100‑1 (MQ=255)

                                                                                    gTGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:3191987/100‑1 (MQ=255)

                                                                                     tGAATTTTCTT‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCATCGCTAACCATTGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:384249/99‑1 (MQ=255)

                                                                                     tGAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt            >  2:2488767/1‑100 (MQ=255)

                                                                                      gAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgaggg           >  2:461213/1‑98 (MQ=255)

                                                                                      gAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAATAGCGAAAGCCACAGATGCGCCtgagtg           >  1:3363066/1‑100 (MQ=255)

                                                                                      gAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTCAAGTGGCGAAAGCCACACATGCGCCtgagtg           <  1:2640494/100‑1 (MQ=255)

                                                                                      gAATTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCACCAGAGTTTCAACATGAAATAGCGAAAGCCACAGATGCGCCtgagt            >  1:187077/1‑99 (MQ=255)

                                                                                        aTTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAATCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:1164260/100‑1 (MQ=255)

                                                                                        aTTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  1:715508/1‑100 (MQ=255)

                                                                                        aTTTTCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:1044978/1‑100 (MQ=255)

                                                                                         ttttCTC‑‑GTTTT‑‑‑CC‑C‑CC‑CCGCCTGCGTCATCCATAGTTTATCCATTTGCGGCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:2737060/99‑1 (MQ=255)

                                                                                         ttttCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:3803882/1‑99 (MQ=255)

                                                                                         ttttCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt        <  1:956634/100‑1 (MQ=255)

                                                                                         ttttCTC‑‑G‑TTT‑‑‑CC‑C‑CCTCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:2736616/99‑1 (MQ=255)

                                                                                           ttCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  2:3102077/1‑100 (MQ=255)

                                                                                           ttCTC‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCAAGCGCCAACCATAGTTTATCAATTTGCGCACTGGTTTCATAGTGAAGTGGCAAAACCCACAGAAGCGCCtcagtcaaggt      >  2:1840128/1‑94 (MQ=255)

                                                                                            tatc‑‑G‑TTTG‑‑CC‑C‑CC‑CCGCCAGCGCCAAGCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAGGTGGCGAAAGCTACAGATGCGCCTGagtgagtg       <  2:2345849/96‑1 (MQ=255)

                                                                                             ctc‑‑G‑TTTC‑‑CC‑C‑CC‑CCTCCAGCGCTAACCATAGTTTATCCATTTGCGCCCGAGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     <  1:289090/99‑1 (MQ=255)

                                                                                             ctc‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    <  2:1231799/100‑1 (MQ=255)

                                                                                               c‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  2:1668996/100‑1 (MQ=255)

                                                                                               c‑‑G‑TTTC‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  2:2802192/1‑100 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑‑G‑TTT‑‑‑CC‑C‑CC‑CCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`