Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,459,293:1 (G)5→6 100% coding (955/1482 nt) gspE → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,459,2881.G91.0% 493.4 / 42.6 156coding (950/1482 nt)gspEgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base . (7/7);  new base G (63/79);  total (70/86)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

GGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCA  >  NC_000913/3459190‑3459382
                                                                                                    |                                                                                                
ggAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCAtggtg                                                                                                 <  1:2426247/100‑1 (MQ=255)
  aaGGCATTGGGCAAACGCAGGTGAATACCCGAGTGGATATGTATTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGAACCCGGATGTCGTCATGGTggg                                                                                              >  2:264087/1‑100 (MQ=255)
   aGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggg                                                                                             >  2:2435394/1‑100 (MQ=255)
   aGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggg                                                                                             <  2:1176903/100‑1 (MQ=255)
     gCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggggg                                                                                           >  1:1564041/1‑99 (MQ=255)
     gCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCACGACCCGGATGTCGTCATGGTgggggg                                                                                           <  1:2385744/100‑2 (MQ=255)
      cATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTAGCCAGGACCCGGATGTCGTCATGGTgggggga                                                                                          >  1:807962/1‑98 (MQ=255)
          gggCAAACGCAGGTGAATACCCGTGTGGATATGTATTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGgaaa                                                                                       >  2:1528657/1‑97 (MQ=255)
           ggCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCACCAAGACCCGGATGTCGTCATGGTGGGGGGAAATTc                                                                                     >  1:745498/1‑100 (MQ=255)
             cAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGt                                                                                   <  1:875662/100‑1 (MQ=255)
             cAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGt                                                                                   <  1:2018410/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTg                                                                                  <  1:3034146/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTg                                                                                  <  1:3079002/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGTCATTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTg                                                                                  <  2:3157669/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGCCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTg                                                                                  <  2:2318129/100‑1 (MQ=255)
               aaCGCAGGTGAATTCCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGa                                                                                 <  2:3759424/100‑1 (MQ=255)
               aaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGa                                                                                 >  2:1738296/1‑100 (MQ=255)
               aaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGa                                                                                 <  2:38364/100‑1 (MQ=255)
                aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGAt                                                                                <  1:1980929/100‑1 (MQ=255)
                aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGAt                                                                                <  1:533009/100‑1 (MQ=255)
                  gCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATAc                                                                              <  2:1977761/100‑1 (MQ=255)
                     ggTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGa                                                                           >  2:721575/1‑100 (MQ=255)
                     ggTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGa                                                                           <  1:3049571/100‑1 (MQ=255)
                       tGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGaaa                                                                         >  2:2493647/1‑100 (MQ=255)
                        gAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATTCTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAAc                                                                        <  1:2965775/100‑1 (MQ=255)
                         aaTACCCGTGTGGATATGTTTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGTACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATAc                                                                              <  2:2972509/93‑1 (MQ=255)
                           tACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGTACCCTGATGTCGTCATGGTGGGGGGAAAATCGTGATACAGAAACcgc                                                                     >  1:1942717/1‑100 (MQ=255)
                           tACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACcgc                                                                     >  1:3887744/1‑100 (MQ=255)
                            aCCCGTGTGGATATGTCTTTCGGTCGCGGCCTGCGTGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACcgcg                                                                    <  1:3656896/100‑1 (MQ=255)
                             cccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACcgcg                                                                    <  1:2727438/99‑1 (MQ=255)
                             cccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGAGGAGGGCAATTCGTGATACAGAAAc                                                                        >  2:3712279/1‑95 (MQ=255)
                              ccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCa                                                                  >  2:2658918/1‑100 (MQ=255)
                              ccGTGTGGAGATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCgcgc                                                                   <  2:3841699/99‑1 (MQ=255)
                                   tgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTAGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTg                                                             >  1:1233821/1‑100 (MQ=255)
                                    ggATATGTCTTTCGCTCGCGGCGTGCGCGCCATAGTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTg                                                             >  2:3898609/1‑99 (MQ=255)
                                    ggATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGc                                                            <  2:591501/100‑1 (MQ=255)
                                    ggATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGc                                                            >  1:3684740/1‑100 (MQ=255)
                                     gATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGAAGGAAATTCGAGATACAGAAACCGCGCAGATTGCg                                                           >  1:470094/1‑100 (MQ=255)
                                     gATATGTCTTTCACTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCATCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCg                                                           >  1:63820/1‑100 (MQ=255)
                                      atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:2822286/100‑1 (MQ=255)
                                      atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:2820630/100‑1 (MQ=255)
                                       tatGTCTTTCGCTCGGGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:3437345/99‑1 (MQ=255)
                                       tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAGCCGCGCAGATTGCgg                                                          <  2:1163057/99‑1 (MQ=255)
                                       tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  1:3561865/99‑1 (MQ=255)
                                       tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGt                                                         <  2:3197010/100‑1 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  2:1680715/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  1:3220493/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  1:2203764/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  1:808900/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    <  1:2135048/100‑1 (MQ=255)
                                             tttCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGc                                                   >  1:397045/1‑100 (MQ=255)
                                             tttCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGc                                                   <  1:3341724/100‑1 (MQ=255)
                                                 gctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGcn                                                  <  2:107784/97‑2 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              <  2:1915769/100‑1 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:12159/1‑100 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:2324356/1‑100 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:660038/1‑100 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              <  1:3270303/100‑1 (MQ=255)
                                                    cgtggtcTGCGCGCTATACTTCGCCAGGAAGCGGATGTCGACATGGTGGGGGGAAAGTCGTGGACCATAAAACGCGACGATTGCGGTTCAg                                                     >  2:2690425/7‑91 (MQ=255)
                                                     gcgGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCa                                           <  1:1390634/100‑1 (MQ=255)
                                                       ggCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCAcc                                         >  1:3623041/1‑100 (MQ=255)
                                                       ggCATGCGCGCCATACTTCGCCAGGACCCGGATTTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCAcc                                         <  2:3802217/100‑1 (MQ=255)
                                                         ccTGTGCGCCATACTTCGCCATGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCgg                                       <  1:2681252/100‑1 (MQ=255)
                                                          cTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGc                                      >  2:2247117/1‑100 (MQ=255)
                                                          cTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGc                                      <  2:3333623/100‑1 (MQ=255)
                                                             cgcgcCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCAt                                   <  1:2957187/100‑1 (MQ=255)
                                                              gcgcCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATc                                  >  2:1312532/1‑100 (MQ=255)
                                                                gcCATACTTCGCCAGAACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCGCGCTCACCg                                        <  2:3754435/92‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTgg                               <  1:1419528/97‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  2:3684701/100‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  1:631834/100‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGACCTCGCTCACCGGCCATCTGGTAc                            >  1:874169/1‑100 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  1:1801001/1‑100 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  1:3119601/1‑100 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAcag                          >  1:2606930/1‑98 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGATCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  1:1086277/1‑100 (MQ=255)
                                                                       ttCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActct                         <  2:1766440/100‑1 (MQ=255)
                                                                       ttCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTACAGGCCTCGCTCACCGGCCATCTGGTActct                         <  2:552011/100‑1 (MQ=255)
                                                                         cGCCAGGACCCGGATGTCGTTATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCa                       <  1:3685930/100‑1 (MQ=255)
                                                                          gCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCACACAAATAGCAAATCAGGACTCGCTCACCGGCCATCTGGTACTCTCaa                      >  1:177384/1‑100 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATGAAGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAac                     >  2:1800319/1‑100 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAac                     <  1:2927895/100‑1 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAac                     <  1:2542879/100‑1 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTACAGATAAGACCTCGCTCACCGGCCATCTGGTACTCTCAac                     >  1:2331898/1‑100 (MQ=255)
                                                                            cAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAaca                    <  2:2705285/100‑1 (MQ=255)
                                                                            cAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATGGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAaca                    >  2:2183922/1‑100 (MQ=255)
                                                                              ggACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACt                  >  1:601900/1‑100 (MQ=255)
                                                                              ggACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACt                  >  1:2089070/1‑100 (MQ=255)
                                                                               gACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTcc                <  2:2931611/100‑1 (MQ=255)
                                                                                 cccGGCTGTCGTCTTTGTGGGGGGAAATTCGTGATACAGAAATCGGGCAGATTGCGGTTCAGGCCTCGCTCACCGTCCATCTTGTACTCTCAACACTCca               >  2:640889/1‑100 (MQ=255)
                                                                                 cccGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCca               >  1:2749076/1‑100 (MQ=255)
                                                                                  ccGGATGTCGTCATGGTGGGGGGAAATTCGTTATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcac              <  1:1150478/100‑1 (MQ=255)
                                                                                  ccGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcac              <  1:1458364/100‑1 (MQ=255)
                                                                                        gtcgtcATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAAc        <  2:712492/100‑1 (MQ=255)
                                                                                          cgtcATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCacac            <  2:801171/94‑1 (MQ=255)
                                                                                          cgtcATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAg      <  2:3664143/100‑1 (MQ=255)
                                                                                          cgtcATGGTGGGGGTAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAg      <  1:577252/100‑1 (MQ=255)
                                                                                            tcATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTg    >  2:2922826/1‑100 (MQ=255)
                                                                                              aTGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGc   <  2:2475946/99‑1 (MQ=255)
                                                                                              aTGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCa  <  2:1552901/100‑1 (MQ=255)
                                                                                                    |                                                                                                
GGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCA  >  NC_000913/3459190‑3459382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: