Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,707,320 A→T 17.1% intergenic (‑196/+2) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,707,3200AT17.1% 437.1 / 76.4 180intergenic (‑196/+2)lepA/rseCback‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base A (67/82);  new base T (12/19);  total (79/101)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 185 total aligned reads displayed.

ACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCA  >  NC_000913/2707221‑2707418
                                                                                                   |                                                                                                  
acactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaa                                                                                                    <  1:2882451/100‑1 (MQ=255)
 cactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATATGGAAACGTCCTCGCATTTGTTATGCAAAATGAAACAAAGCCAGTGaaa                                                                                                   >  2:3555154/1‑100 (MQ=255)
   ctacaACATTGGGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCATCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                 <  2:3277601/100‑1 (MQ=255)
   ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                 <  1:1366003/100‑1 (MQ=255)
     acaacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCAc                                                                                               >  2:2402008/1‑100 (MQ=255)
        acaTTGAGGCGAGGAAATGTTCATACCTTATTGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTgg                                                                                            <  1:360371/100‑1 (MQ=255)
        acaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATACAAAATGCAACAAAGCCAGTGAAATCACTg                                                                                             >  1:999765/1‑99 (MQ=255)
         caTTGAGGCTAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGc                                                                                           <  2:1874992/100‑1 (MQ=255)
         caTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGc                                                                                           <  1:400712/100‑1 (MQ=255)
         caTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGc                                                                                           <  2:2727310/100‑1 (MQ=255)
          aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCt                                                                                          >  1:2955249/1‑100 (MQ=255)
           ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAATGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtt                                                                                         <  1:2185735/100‑2 (MQ=255)
            tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcg                                                                                        <  1:1767085/100‑1 (MQ=255)
            tGAGGCGAAGAAATGTTCATATCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTGGTGATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                        <  1:2065242/100‑3 (MQ=255)
             gAGGCGAGGAAATGTTTATACCGTATTGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGTAACAAAGCCAGTGATTTCACTGGCtttg                                                                                       <  1:1865346/100‑4 (MQ=255)
             gAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGCCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTc                                                                                         >  1:2782868/1‑98 (MQ=255)
              aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgt                                                                                      <  1:237867/100‑5 (MQ=255)
               ggCGAGGAAATTTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgt                                                                                      <  1:3887425/99‑5 (MQ=255)
               ggCGAGGAAATGTTCATACCTTATTGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgt                                                                                      <  1:220366/99‑5 (MQ=255)
               ggCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcgcg                                                                                      >  1:1558623/1‑99 (MQ=255)
               ggCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGt                                                                                     >  2:1862587/1‑100 (MQ=255)
               ggCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGt                                                                                     <  1:2614851/100‑1 (MQ=255)
                gCGATGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAAGCACTGGCTcgcg                                                                                      <  2:3841329/98‑1 (MQ=255)
                gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttg                                                                                    <  1:132092/100‑7 (MQ=39)
                gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc                                                                                    <  1:1367886/100‑1 (MQ=255)
                gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc                                                                                    >  1:3679168/1‑100 (MQ=255)
                 cGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgn                                                                                      <  1:3670550/97‑5 (MQ=255)
                  gAGGAAATGTTCATACCGTATTGATTGTGGTATTTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttgca                                                                                  <  1:1689774/100‑9 (MQ=37)
                   aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCtt                                                                                  <  2:1586539/99‑1 (MQ=255)
                   aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTc                                                                                 >  2:3802557/1‑100 (MQ=255)
                    ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                <  2:406717/100‑1 (MQ=255)
                    ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                <  1:1202191/100‑1 (MQ=255)
                    ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                <  2:1012038/100‑1 (MQ=255)
                     gAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCTCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                <  1:1159871/99‑1 (MQ=255)
                      aaaTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGa                                                                              <  1:3220083/100‑1 (MQ=255)
                         tGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                           >  1:2046569/1‑100 (MQ=255)
                         tGTTCATACCGTATGGATTGTGGTATATGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                           >  2:1770594/1‑100 (MQ=255)
                            tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGt                                                                        <  2:2118337/100‑1 (MQ=255)
                            tCATACCGTATGGATTGTGGTATCAGGAAAAGTACTCGCATTTGTTATGCAAAATGTAACAAAGCCAGTGAAATCACTGACTCGCGTCTTCCGAAGATGt                                                                        >  2:858938/1‑100 (MQ=255)
                             cATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGtt                                                                       <  1:139479/100‑1 (MQ=255)
                                 ccGTATGGATTGTGGTATCTGGAATCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCTCATCTTCCGAAGATGTTTCa                                                                    <  2:1236955/99‑1 (MQ=255)
                                 ccGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCaa                                                                   >  1:604316/1‑100 (MQ=255)
                                  cGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACCAAGCCAGTGAAATCACTGGCTCGCGTCTTACGAAGATTTTTCaaa                                                                  >  2:3336894/1‑100 (MQ=255)
                                   gTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAAt                                                                 <  2:792647/100‑1 (MQ=255)
                                      tctaTTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGc                                                              <  1:1939099/97‑1 (MQ=255)
                                       ggATTGTGGTATCTGGAAACGTCCACGCATTTGTTATGCAAAATGCAAAAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGca                                                             >  2:2489054/1‑100 (MQ=255)
                                        gATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGcac                                                            <  2:1465911/100‑1 (MQ=255)
                                        gATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGca                                                             <  1:879638/99‑1 (MQ=255)
                                         aTTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGcaga                                                           >  2:1939083/1‑98 (MQ=255)
                                            gtgGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAgg                                                        >  1:3519483/1‑100 (MQ=255)
                                            gtgGTATCTGGAAACGTCCTCGCAGTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAgg                                                        <  2:2763860/100‑1 (MQ=255)
                                             tgGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGc                                                       <  2:1300113/100‑1 (MQ=255)
                                             tgGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGc                                                       >  1:819503/1‑100 (MQ=255)
                                              ggTATCTTGAATCGTCCTCTCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                      <  1:2277473/100‑1 (MQ=255)
                                                tATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAg                                                    >  1:3371714/1‑100 (MQ=255)
                                                  tCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggc                                                  >  1:556665/1‑100 (MQ=255)
                                                    tGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAgg                                                   >  2:291899/1‑97 (MQ=255)
                                                    tGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGAGAAATCACTGGCTCGCGTCTTACGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                >  2:2688103/1‑100 (MQ=255)
                                                        aaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCTAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAt                                            <  1:96943/100‑1 (MQ=255)
                                                        aaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCaan                                            <  1:3198445/100‑2 (MQ=255)
                                                        aaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAt                                            >  1:2007698/1‑100 (MQ=255)
                                                        aaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAt                                            >  2:1675464/1‑100 (MQ=255)
                                                         aCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATg                                           <  2:3902109/100‑1 (MQ=255)
                                                         aCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATg                                           <  2:328916/100‑1 (MQ=255)
                                                         aCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATg                                           <  1:2441136/100‑1 (MQ=255)
                                                          cttCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATg                                           <  1:3746634/97‑1 (MQ=255)
                                                                cGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCt                                    >  2:2201332/1‑100 (MQ=255)
                                                                 gCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCtt                                   <  1:1035233/100‑1 (MQ=255)
                                                                      tGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgat                              <  1:3853176/100‑1 (MQ=255)
                                                                      tGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAga                               >  1:1460438/1‑99 (MQ=255)
                                                                         tATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAgatgat                           >  2:631948/1‑100 (MQ=255)
                                                                           tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCg                         >  2:3661220/1‑100 (MQ=255)
                                                                           tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGACAATACCACGCTTAAGATGATc                          >  1:3923692/1‑99 (MQ=255)
                                                                             cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGt                       >  1:724453/1‑100 (MQ=255)
                                                                             cAAAATGCAACAAAGCCAGAGAAATCACTGGGTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCGACGCTTAAGATGATCgg                        >  2:3122626/1‑99 (MQ=255)
                                                                              aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGtt                      <  2:191204/100‑1 (MQ=255)
                                                                               aaaTGTAATAAAGCCAGTGAAATCACTGGCTAGCGTTTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTg                     <  1:3428665/100‑1 (MQ=255)
                                                                               aaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTg                     >  2:2359693/1‑100 (MQ=255)
                                                                                aaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGc                    <  1:2727513/100‑1 (MQ=255)
                                                                                    gacgcgaGCCAGTGATTTCACTGGATAGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGAACGGTTGCCAtt                >  2:338508/7‑100 (MQ=255)
                                                                                    caacaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCAtt                >  1:532207/1‑100 (MQ=255)
                                                                                     aacaaAGCCAGTTATATCACTGGCTCGTTTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc               <  1:3579464/100‑1 (MQ=255)
                                                                                     aacaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAATATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc               <  2:1567418/100‑1 (MQ=255)
                                                                                     aacaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCACACAAAGCCAGACGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc               >  1:2028611/1‑100 (MQ=255)
                                                                                      cgcgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCt              >  2:3011633/5‑100 (MQ=255)
                                                                                       gcgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGACACGCTTAAGATGATCGGTTGCCATTCTg             >  1:1150661/4‑100 (MQ=255)
                                                                                        cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc            >  1:2416944/3‑100 (MQ=255)
                                                                                        cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc            >  1:3615823/3‑100 (MQ=255)
                                                                                        cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAAGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTg             >  1:1799622/3‑99 (MQ=255)
                                                                                        cgaGCCAGTGATTTCACTGGCTAGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAGGCCACGCTTAAGATGATCGGATACCATTCTg             >  2:262832/3‑99 (MQ=255)
                                                                                           gCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCTATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg         <  2:2087817/100‑1 (MQ=255)
                                                                                           gCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg         >  2:2178142/1‑100 (MQ=255)
                                                                                           gCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg         <  1:150590/100‑1 (MQ=255)
                                                                                              aGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgc      <  2:2448873/100‑1 (MQ=255)
                                                                                              aGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgc      <  2:1792473/100‑1 (MQ=255)
                                                                                              aGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGACAAAGCAGCAATGCGACGATTAAGATGATCGGTTGCCATACTGCCCGAgc      >  1:3128147/1‑100 (MQ=255)
                                                                                              aGTGAAATCACTGGCTAGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgc      <  2:3750371/100‑1 (MQ=255)
                                                                                               gTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgca     <  2:1091472/100‑1 (MQ=255)
                                                                                                tGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgcag    <  1:1784552/100‑1 (MQ=255)
                                                                                                  aaaTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgcagca  <  1:1038081/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
ACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCA  >  NC_000913/2707221‑2707418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: