BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	410,479	A→G	100%	S112S (TCA→TCG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,479	0	A	G	99.4%	582.0 / ‑5.1	167	S112S (TCA→TCG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (78/87); total (79/88)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 167 total aligned reads displayed.

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGC  >  NC_000913/410382‑410574

cAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGa                                                                                               <  1:2953689/100‑1 (MQ=255)

 aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                              >  2:3234576/1‑100 (MQ=255)

 aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                              <  2:3384486/100‑1 (MQ=255)

 aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGa                                                                                               <  2:2131178/99‑1 (MQ=255)

 aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGGCTGGCGGTTTCGGaa                                                                                              >  1:3869605/1‑100 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  1:3623393/100‑1 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  2:423555/100‑1 (MQ=255)

     tGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAAGGACGCTAACTGTCTGGCGGTTTCGGAAGCAg                                                                                          <  1:1220983/100‑1 (MQ=255)

      ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATCACGCTAACTGTCTGGCGTTTTCGGAAGCaat                                                                                         <  1:2635998/100‑3 (MQ=255)

        cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                       >  1:2707239/1‑100 (MQ=255)

        cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                       >  2:403733/1‑100 (MQ=255)

         tCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                      >  1:3307189/1‑100 (MQ=255)

         tCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                      <  1:1267550/100‑1 (MQ=255)

         tCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                      >  2:31715/1‑100 (MQ=255)

          cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                     >  2:1893140/1‑100 (MQ=255)

          cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                     >  1:2731259/1‑100 (MQ=255)

          cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                     <  1:3481233/100‑1 (MQ=255)

           aaCGGTCAGCCATTCGATAAAGACTTAAGCGAGAGGTTGCAGCGGTAAGTGCGGCTGGCAAATGACTCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                      <  2:884879/98‑1 (MQ=255)

            aCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATgg                                                                                   >  1:1012056/1‑100 (MQ=255)

            aCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATgg                                                                                   >  1:1918103/1‑100 (MQ=255)

             cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATgg                                                                                   >  2:2141973/1‑99 (MQ=255)

              ggTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcg                                                                                 >  1:3164214/1‑100 (MQ=255)

               gTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcgc                                                                                <  2:2443893/100‑1 (MQ=255)

                 cAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCgg                                                                              >  2:3799046/1‑100 (MQ=255)

                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                            <  1:3350699/100‑1 (MQ=255)

                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTATCGGAAGCAGTAGATGGAGCggaa                                                                            >  2:2773120/1‑98 (MQ=255)

                    ccATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAg                                                                           >  2:3076120/1‑100 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                         <  1:2311268/100‑1 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                         <  2:562915/100‑1 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                         <  2:1713317/100‑1 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCGGGCGGTTTCGGAAGCAGTAGATGGTGCGGCAGCg                                                                         <  1:3501506/100‑1 (MQ=255)

                       ttCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCgg                                                                        >  2:3068178/1‑100 (MQ=255)

                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                      >  1:685044/1‑100 (MQ=255)

                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                      <  1:360027/100‑1 (MQ=255)

                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                      >  2:3031990/1‑100 (MQ=255)

                          gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGATTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                     >  1:1287347/1‑100 (MQ=255)

                           aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCCAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgc                                                                    <  1:29232/100‑1 (MQ=255)

                             naaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgc                                                                  >  1:1508174/2‑100 (MQ=255)

                             aaaGACTTAAGCGCGTGGTTGCAGCGGGAAGGGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgc                                                                  <  2:2968207/100‑1 (MQ=255)

                             aaaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgc                                                                  >  2:633655/1‑100 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                 >  2:2887486/1‑100 (MQ=255)

                               aGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAg                                                                >  1:2083016/1‑100 (MQ=255)

                                  cgtAAGCGCGAGGTTGCAGCGGGAAGTGCGGGTGGCAAATGACGCTAACTGGCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGAc                                                              <  2:758915/97‑1 (MQ=255)

                                  cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                             >  2:3130182/1‑100 (MQ=255)

                                  cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTAGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                             >  2:3682236/1‑100 (MQ=255)

                                   ttAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACgg                                                            <  2:3850493/100‑1 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTGCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGt                                                           >  2:2659324/1‑100 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGAGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCTGTAGATGGCGCGGCAGCGGGAGCGCAGACGGt                                                           >  2:40200/1‑100 (MQ=255)

                                            aGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCg                                                   <  2:3456590/100‑1 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  2:3925939/100‑1 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  2:386571/100‑1 (MQ=255)

                                              gTTGCAGCGGGCAGTGCGGCGGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGGAGATGGCGCGGCAGCGGGAGCGCAGACGGTATGTGCCGTg                                                 >  2:2395490/1‑100 (MQ=255)

                                               ttGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGa                                                <  2:2553481/100‑1 (MQ=255)

                                               ttGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGa                                                <  1:3885499/100‑1 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAt                                               <  1:2501972/100‑1 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCAGCAACGGGAACGCAGACGGTATTTGCCGTGa                                                >  1:2464228/1‑99 (MQ=255)

                                                  cAGCGGGAAGTGCGGCTGGCAAATGATGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCCCAGACGGTATTTGCCGTGATTa                                             <  2:1928408/100‑1 (MQ=255)

                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCTGGAGCGCAGACGGTATTTGCCGTGATTATc                                           <  2:3584526/100‑1 (MQ=255)

                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATc                                           >  2:3316440/1‑100 (MQ=255)

                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATc                                           <  1:2890003/100‑1 (MQ=255)

                                                     cGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCg                                          <  1:550218/100‑1 (MQ=255)

                                                      gggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCgg                                         <  1:2887512/100‑1 (MQ=255)

                                                      gggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCgg                                         <  1:3835124/100‑1 (MQ=255)

                                                       ggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGc                                        <  1:3208273/100‑1 (MQ=255)

                                                       ggAAGGGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGc                                        <  2:3500296/100‑1 (MQ=255)

                                                        gAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCa                                       >  2:1538051/1‑100 (MQ=255)

                                                           gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                    <  1:403739/100‑1 (MQ=255)

                                                             gCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGa                                  >  2:298726/1‑100 (MQ=255)

                                                              cGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAt                                 <  1:3913894/100‑1 (MQ=255)

                                                               ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATg                                >  1:2054584/1‑100 (MQ=255)

                                                               ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATg                                <  1:250988/100‑1 (MQ=255)

                                                                gCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgc                               <  1:3223439/100‑1 (MQ=255)

                                                                 cTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcg                              >  1:2084773/1‑100 (MQ=255)

                                                                  tGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcgg                             >  2:498055/1‑100 (MQ=255)

                                                                    gCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcggcg                           >  1:3090293/1‑100 (MQ=255)

                                                                     cAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgcgc                          >  1:478316/1‑100 (MQ=255)

                                                                     cAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgcgc                          >  1:3041458/1‑100 (MQ=255)

                                                                     cAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgcgc                          >  1:906386/1‑100 (MQ=255)

                                                                        aTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCggg                       <  2:1062842/100‑1 (MQ=255)

                                                                         tGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGc                      >  1:2214279/1‑100 (MQ=255)

                                                                          gACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCAGCGCGAGCg                     >  1:3531151/1‑100 (MQ=255)

                                                                            cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                   <  1:866258/100‑1 (MQ=255)

                                                                             gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                  <  1:1055336/100‑1 (MQ=255)

                                                                              cTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                 <  1:226901/100‑1 (MQ=255)

                                                                                aaCTGTCTGGCGGTTTCTGAAGCAGTAGATGGCGCGGCAGCGGGATCGCAGACGGTATTCGCCGTGATTTTGGCCACTGGATGCGGCGCGGGCGTGGCAt               <  2:3645525/100‑1 (MQ=255)

                                                                                  cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGTGGCGCGGGCGTGGCATTc             >  2:6938/1‑100 (MQ=255)

                                                                                  cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGGGGCATTc             <  2:1618734/100‑1 (MQ=255)

                                                                                   tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa            >  2:1887464/1‑100 (MQ=255)

                                                                                   tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa            <  1:93771/100‑1 (MQ=255)

                                                                                   tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa            <  1:2990654/100‑1 (MQ=255)

                                                                                    gTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCaa           <  2:1633925/100‑1 (MQ=255)

                                                                                    gTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCATGGCATTc             <  2:3188198/98‑1 (MQ=255)

                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAt          >  1:1941155/1‑99 (MQ=255)

                                                                                      cTGCCGGTTTCGGAAGCCGTCGATGCCACGGCCGCGTGAGCGCAGACGGTATTTGCCGTGATTAGCCGCACGGGATGCGGCGCGGGCGTGGCATTCa            <  2:2332487/97‑1 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg       <  1:968747/100‑1 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCAGTGATTATCGCCACGGGACGCGGCGCGGGCCTGGGATTCAAggg        >  2:3556087/1‑99 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGAAGCGCAGACGGTATTTGCAGTGATTATCGGCACGGGATGCGGCGTGGGCGTGGCATTCAATggg       <  2:963286/100‑1 (MQ=255)

                                                                                          cGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcg     >  2:1233543/1‑100 (MQ=255)

                                                                                            gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCATGGGATGCGGCGCGGGCGTGGCATTCAATgtgcgg    >  2:118108/1‑99 (MQ=255)

                                                                                            gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg   <  1:2824538/100‑1 (MQ=255)

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGC  >  NC_000913/410382‑410574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`