BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	433,246	A→G	25.5%	D82G (GAT→GGT)	nrdR →	Nrd regulon repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	433,246	0	A	G	25.5%	255.3 / 99.5	162	D82G (GAT→GGT)	nrdR	Nrd regulon repressor
Reads supporting (aligned to +/- strand): ref base A (60/59); new base G (21/20); total (82/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 165 total aligned reads displayed.

CGCGTGTTGTAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGATGGAGC  >  NC_000913/433147‑433344

cgcgTGTTGTAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGa                                                                                                     >  1:118416/1‑100 (MQ=255)

cgcgTGTTGTAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGa                                                                                                     >  2:1404515/1‑100 (MQ=255)

  cgTGTTGTAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATg                                                                                                   <  2:1033958/100‑1 (MQ=255)

    tgttgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCTGGTGAGTTCCGATGAc                                                                                                 <  2:1554803/100‑1 (MQ=255)

    tgttgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGan                                                                                                 <  2:1769049/100‑2 (MQ=255)

    tgttgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGAc                                                                                                 >  1:1170071/1‑100 (MQ=255)

     gttgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACg                                                                                                <  1:2558937/100‑1 (MQ=255)

      ttgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGt                                                                                               >  1:624486/1‑100 (MQ=255)

       tgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTc                                                                                              >  2:1095860/1‑100 (MQ=255)

       tgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTc                                                                                              >  2:3681508/1‑100 (MQ=255)

       tgtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTc                                                                                              >  2:3255752/1‑100 (MQ=255)

        gtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCg                                                                                             >  2:2183655/1‑100 (MQ=255)

        gtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCg                                                                                             <  1:2218009/100‑1 (MQ=255)

        gtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCg                                                                                             >  1:2802116/1‑100 (MQ=255)

        gtAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGAGGGCGCTGGAAAAACGTCCGATGAGTTCAGGTGACGTCg                                                                                             >  1:1925192/1‑100 (MQ=255)

         tAAAAAGCAACGACGTGCGTGAATCATTTTATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGa                                                                                            <  1:1757349/100‑1 (MQ=255)

         tAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGTAATGCTGCGGCCACTGGAAAAACGTCCGGTGAGATCAGGTGAc                                                                                                 >  1:3235257/1‑95 (MQ=255)

         tAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGa                                                                                            <  1:2832272/100‑1 (MQ=255)

          aaaaaGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGAAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGaa                                                                                           >  1:2974965/1‑100 (MQ=255)

           aaaaGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGaaa                                                                                          <  2:1779964/100‑1 (MQ=255)

            aaaGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAAt                                                                                         <  1:3016637/100‑1 (MQ=255)

              aGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATgg                                                                                       <  1:3519430/100‑1 (MQ=255)

              aGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATgg                                                                                       <  1:560402/100‑1 (MQ=255)

              aGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATgg                                                                                       <  1:2974019/100‑1 (MQ=255)

               gCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGc                                                                                      <  1:3681547/100‑1 (MQ=255)

               gCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGc                                                                                      <  1:1615582/100‑1 (MQ=255)

               gCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGc                                                                                      >  2:3090076/1‑100 (MQ=255)

                  acgacgTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGcaat                                                                                   >  1:3768763/1‑100 (MQ=255)

                   cgacgTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGcaatc                                                                                  >  1:731228/1‑100 (MQ=255)

                   cgacgTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGcaatc                                                                                  <  2:3242634/100‑1 (MQ=255)

                   cgacgTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGcaatc                                                                                  <  1:3394082/100‑1 (MQ=255)

                   cgacgTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGcaatc                                                                                  >  1:589610/1‑100 (MQ=255)

                        tgcgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAatcaatca                                                                             <  1:3248966/100‑1 (MQ=255)

                        tgcgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAatcaatca                                                                             >  2:1926885/1‑100 (MQ=255)

                        tgcgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGAGAGTTCCGATGACGTCGAAATGGCAatcaatca                                                                             >  1:1018464/1‑100 (MQ=255)

                        tgcgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGAGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAatcaatca                                                                             >  1:1019593/1‑100 (MQ=255)

                         gcgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCat                                                                            >  1:2178037/1‑100 (MQ=255)

                         gcgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCat                                                                            <  2:2491189/100‑1 (MQ=255)

                          cgtgAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCata                                                                           <  2:2291964/100‑1 (MQ=255)

                             gAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTa                                                                        <  2:2613679/100‑1 (MQ=255)

                               aCCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTaaa                                                                      >  2:3277972/1‑100 (MQ=255)

                               aCCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTaaa                                                                      <  2:2231350/100‑1 (MQ=255)

                                ccGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAAt                                                                     >  1:2901073/1‑100 (MQ=255)

                                 cGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATc                                                                    <  1:907887/100‑1 (MQ=255)

                                  gTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCg                                                                   >  1:2004544/1‑100 (MQ=255)

                                  gTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCg                                                                   <  1:1988422/100‑1 (MQ=255)

                                   tttAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGTGTTCCGGTGATGTCGAAATGGCAATCAATCATATTAAATCGc                                                                  <  1:2649001/100‑1 (MQ=255)

                                    ttAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCa                                                                 <  1:2912875/100‑1 (MQ=255)

                                      aaTGAAGAGAAATTGCGTAGCGGAATGCTGCTGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGc                                                               <  2:3744288/100‑1 (MQ=255)

                                       aTGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCt                                                              >  2:2003890/1‑100 (MQ=255)

                                        tGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATAACGTCGAAATGGAAATCAATCATATTAAATCGCAGCTg                                                             >  1:3083784/1‑100 (MQ=255)

                                         gAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTg                                                             >  2:3821973/1‑99 (MQ=255)

                                          aaGAGACATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGGCCGGTGAGTGCCGATGACGTCGAAATGGAAATCAAGCATATTCAATc                                                                    >  2:3210242/1‑91 (MQ=255)

                                           agagAACTTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGATCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTgcgc                                                          >  2:81214/1‑100 (MQ=255)

                                             tgaAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTgcgcgc                                                        <  2:512513/99‑1 (MQ=255)

                                              gaAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCAGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGc‑c                                                      >  1:2388016/1‑100 (MQ=255)

                                                 aTTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CAcc                                                   <  1:1398468/100‑1 (MQ=255)

                                                     cGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTg                                               >  1:697829/1‑100 (MQ=255)

                                                      gTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGa                                              >  1:3053882/1‑100 (MQ=255)

                                                      gTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGa                                              <  2:2727258/100‑1 (MQ=255)

                                                       tAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGAGC‑CACCGGTGAg                                             >  2:130376/1‑100 (MQ=255)

                                                            gAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGa                                        <  2:3628892/100‑1 (MQ=255)

                                                              aTGCTGCGGGCGCTGGATAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAATTCGCAGCTGCGC‑T‑CACCGGTG‑GCGCGCAgc                                     <  2:1516256/99‑2 (MQ=255)

                                                              aTGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGATGTCTATATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAg                                      <  1:136451/100‑1 (MQ=255)

                                                                gctgcGGGCGCTGGTAAACCGTCCGGTGATTTCTGTTTACGTGGAAATGGTAATCAATCAGATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTg                                    <  1:1132026/100‑1 (MQ=255)

                                                                gctgcGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGC‑T‑CACCGGTG‑Gcgcgcagctgc                                  <  2:1367701/100‑8 (MQ=255)

                                                                 ctgcGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGc                                   >  2:3703045/1‑100 (MQ=255)

                                                                 ctgcGGGCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGc                                   >  2:3703391/1‑100 (MQ=255)

                                                                  tgcGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGcc                                  <  2:3615820/100‑1 (MQ=255)

                                                                   gcGGGCGCTGGAAAAACGTCCGGTGAGTTTTGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGCTCACCGGTG‑Gcgcgcagctgcg                                 <  1:3262048/100‑9 (MQ=37)

                                                                   gcGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGTGCGCGAAGTGCCg                                 <  1:2657362/100‑1 (MQ=255)

                                                                   gcGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTccc                                  >  2:2401161/1‑99 (MQ=255)

                                                                     gggCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAg                               <  2:2319419/100‑1 (MQ=255)

                                                                     gggCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAg                               <  2:2697022/100‑1 (MQ=255)

                                                                      ggCGCTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTTCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGc                              <  2:587173/100‑1 (MQ=255)

                                                                        cgcTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCaa                            >  1:1785429/1‑100 (MQ=255)

                                                                         gcTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAg                           >  1:1759223/1‑100 (MQ=255)

                                                                          cTGGAAAAACGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAga                          >  1:3357143/1‑100 (MQ=255)

                                                                           tGGAAAAACGTCCGGTGAGTTCCGATGACATCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAgat                         <  1:31263/100‑1 (MQ=255)

                                                                            ggAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAgatg                        >  1:3441012/1‑100 (MQ=255)

                                                                             gAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAgatga                       <  2:2807484/100‑1 (MQ=255)

                                                                             gAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAgatga                       >  1:3791296/1‑100 (MQ=255)

                                                                              aaaaaCGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAgatgat                      >  2:756943/1‑100 (MQ=255)

                                                                               aaaaCGTCCGGTGAGTTCCGGTGACGTCGAAATGGCAATCAATCATATTAAATCACAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGACGAGCAAgatga                       >  1:1572803/1‑98 (MQ=255)

                                                                                 aaCGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTgg                   >  2:662850/1‑100 (MQ=255)

                                                                                  aCGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGTGCTAAGTGCCGAGCAAGATGATTGGc                  <  2:1668587/100‑1 (MQ=255)

                                                                                  aCGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGc                  >  2:3492540/1‑100 (MQ=255)

                                                                                  aCGTCCGGTGAGTTCCGATGACGTCGAAACGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACGGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGc                  <  2:2344170/100‑1 (MQ=255)

                                                                                   cGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCACGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCa                 >  1:450496/1‑100 (MQ=255)

                                                                                    gTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCaa                >  2:1248776/1‑100 (MQ=255)

                                                                                      ccGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATc              >  1:2060800/1‑100 (MQ=255)

                                                                                        ggTGAGTTCCGATGACGTAGAAATGGCAATAAATAATATTAAATAACAGCTGCGCGC‑CAACGGTGAGCGCGAAATGCCTAGCAAGATGATTGGCAATCTg            >  2:1924495/1‑100 (MQ=255)

                                                                                          tGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGt          >  1:251246/1‑100 (MQ=255)

                                                                                             gTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGAt       <  1:1615776/100‑1 (MQ=255)

                                                                                             gTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGAt       >  2:2704888/1‑100 (MQ=255)

                                                                                              ttCCGATGACATCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGacg      >  2:2816166/1‑98 (MQ=255)

                                                                                               tCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAAAGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGATgg     >  1:998715/1‑100 (MQ=255)

                                                                                                ccGGTGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGATGGa    >  1:389477/1‑100 (MQ=255)

                                                                                                  gATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACTGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGATGGAGc  <  2:2552406/100‑1 (MQ=255)

                                                                                                  gATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGATGGAGc  <  1:3828692/100‑1 (MQ=255)

CGCGTGTTGTAAAAAGCAACGACGTGCGTGAACCGTTTAATGAAGAGAAATTGCGTAGCGGAATGCTGCGGGCGCTGGAAAAACGTCCGGTGAGTTCCGATGACGTCGAAATGGCAATCAATCATATTAAATCGCAGCTGCGCGC‑CACCGGTGAGCGCGAAGTGCCGAGCAAGATGATTGGCAATCTGGTGATGGAGC  >  NC_000913/433147‑433344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`