BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,352,673	T→C	100%	E118G (GAA→GGA)	arcB ←	aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,352,673	0	T	C	100.0%	483.6 / ‑6.7	146	E118G (GAA→GGA)	arcB	aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (80/64); minor base A (2/0); total (82/64)

Only 100 of 147 total aligned reads displayed.

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGAC  >  NC_000913/3352576‑3352769

gAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                                <  2:2513531/100‑1 (MQ=255)

 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                               <  1:115498/100‑1 (MQ=255)

 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                               <  1:257727/100‑1 (MQ=255)

  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                              <  1:1721726/100‑1 (MQ=255)

   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAATTCTGCTTCCGCTTTTTCAAGAACGGCAATTCCCTGa                                                                                             >  1:2582537/1‑100 (MQ=255)

    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                             <  1:2505667/99‑1 (MQ=255)

    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                            <  1:942830/100‑1 (MQ=255)

    tGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAt                                                                                            >  1:1671042/1‑100 (MQ=255)

     gAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAtt                                                                                           <  2:1177402/100‑1 (MQ=255)

     gAATTTGTGTCTCTTCGCGCTATTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTTTGCTTTCGCTTTTTCACGAACGGAAATTCCCTGAtt                                                                                           >  2:1465257/1‑100 (MQ=255)

      aaTTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGAttt                                                                                          <  1:2916550/100‑1 (MQ=255)

        tttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAg                                                                                        <  2:404778/100‑1 (MQ=255)

         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTTAttt                                                                                          >  2:725323/1‑97 (MQ=255)

         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTCCCGCTTTTTCACGAACGACAATTCCCTGATTTAGc                                                                                       >  1:2689078/1‑100 (MQ=255)

          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                      >  2:806704/1‑100 (MQ=255)

           gtgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTg                                                                                     <  1:1560200/100‑1 (MQ=255)

             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                   >  1:2084505/1‑100 (MQ=255)

             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                   >  1:2247208/1‑100 (MQ=255)

              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                  <  1:1978009/100‑1 (MQ=255)

               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                 <  1:2909283/100‑1 (MQ=255)

               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                 <  2:121291/100‑1 (MQ=255)

               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACAAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                 >  1:2226983/1‑100 (MQ=255)

                tctTCGCGCTCTTTGATTACAATTTTCAGTTGGACGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCGGATTTAGCTGGGCaa                                                                                >  2:1897382/1‑100 (MQ=255)

                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                             >  2:1083389/1‑100 (MQ=255)

                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                             >  2:3375659/1‑100 (MQ=255)

                     gcgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatt                                                                            >  2:625435/1‑99 (MQ=255)

                      cgcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtattat                                                                          <  2:2677359/100‑1 (MQ=255)

                        ctctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCt                                                                        <  1:3376284/100‑1 (MQ=255)

                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                      >  2:821973/1‑100 (MQ=255)

                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                      <  2:1361655/100‑1 (MQ=255)

                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                     <  1:2531047/100‑1 (MQ=255)

                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                     <  1:449789/100‑1 (MQ=255)

                            ttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTTTTTTATTTCTATTCCCTGATTTAGCTGGGCAATATTATCTTTTa                                                                    >  2:3169179/1‑100 (MQ=255)

                            ttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTa                                                                    <  2:3619158/100‑1 (MQ=255)

                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                  >  1:1811266/1‑100 (MQ=255)

                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                  <  2:2453552/100‑1 (MQ=255)

                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAGATTATATTTTAAc                                                                  >  1:2274858/1‑100 (MQ=255)

                               aTTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACt                                                                 >  1:2155591/1‑100 (MQ=255)

                                tttCAATTTTCAGTTGGTCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTTTTTTTTTGTAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                                >  2:1613751/1‑100 (MQ=255)

                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                  <  2:1432705/98‑1 (MQ=255)

                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                                >  2:3889641/1‑100 (MQ=255)

                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                                >  1:1337232/1‑100 (MQ=255)

                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                                >  1:37093/1‑100 (MQ=255)

                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                              >  1:1391991/1‑100 (MQ=255)

                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTAACTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                              >  1:3423051/1‑99 (MQ=255)

                                    aaTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTACCTGAACg                                                            <  2:2689054/100‑1 (MQ=255)

                                    aaTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACg                                                            >  2:3628038/1‑100 (MQ=255)

                                         tCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACTAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAg                                                       <  2:1789797/100‑1 (MQ=255)

                                          cAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAgg                                                      <  2:2527933/100‑1 (MQ=255)

                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCCCGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAgg                                                      >  1:2816688/1‑99 (MQ=255)

                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTATTAACTGAACGTTGAGGc                                                     <  1:1823841/100‑1 (MQ=255)

                                             ttggttgtaGGTTTCTTGTAGTTTTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTc                                                   <  2:3459845/92‑1 (MQ=255)

                                             ttGGTCTAAAGTTTCTTGCAGTTCTTCTTCCGCTTTTTCACGATCGTTAATTCCCTTATTTAGTTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                     <  2:2363307/98‑1 (MQ=255)

                                               ggCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaa                                                 >  2:1036603/1‑100 (MQ=255)

                                                gCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACTAATTTCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaaa                                                >  2:2969090/1‑100 (MQ=255)

                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaag                                                >  2:2371432/1‑98 (MQ=255)

                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                               >  2:778978/1‑100 (MQ=255)

                                                   gAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcg                                             >  1:1006415/1‑100 (MQ=255)

                                                      ggTTTCCTGCAGTTTTGCTTTCGTTTTTTCATGAACGGGAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcg                                             <  1:678869/97‑1 (MQ=255)

                                                      ggTTTCCTGCAGTTCTGCATCCACTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCACTATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgc                                            >  2:1715780/1‑98 (MQ=255)

                                                       gTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGATCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgct                                         <  2:437552/100‑1 (MQ=255)

                                                       gTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgct                                         >  1:3407885/1‑100 (MQ=255)

                                                         ttCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcg                                       <  1:778987/100‑1 (MQ=255)

                                                         ttCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcg                                       <  2:3750431/100‑1 (MQ=255)

                                                         ttCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcg                                       <  1:3628077/100‑1 (MQ=255)

                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAGCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgagatcggaa                                    >  2:3706032/1‑91 (MQ=255)

                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                    <  2:1256295/100‑1 (MQ=255)

                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                    <  1:1028199/100‑1 (MQ=255)

                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                    >  1:557092/1‑100 (MQ=255)

                                                                 gTTCTGCTTCCGCTTTTTCACGAACTGCAATTCCCTGATTTAGCTGGGCAATATTATATTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                    <  1:3126688/95‑1 (MQ=255)

                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCAtctc                               <  2:2784325/100‑1 (MQ=255)

                                                                 gTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATAGCGCTCGCGCAtctc                               >  1:3878394/1‑100 (MQ=255)

                                                                  tgcTGCTTCCGCTCTTTCACGAACGGCAATTCCATGATTTAGCTAGGCAATACTATCTTTTAACTCCCCGTTGCGCCTCAAATCGCGCTCGCGCATctcc                              <  2:2737370/98‑1 (MQ=255)

                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcct                             >  1:842978/1‑100 (MQ=255)

                                                                   tCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATctcct                             >  1:2337596/1‑100 (MQ=255)

                                                                     tGCTTCCGTTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                           <  2:4656/100‑1 (MQ=255)

                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                           >  1:2186400/1‑100 (MQ=255)

                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                           <  1:220657/100‑1 (MQ=255)

                                                                     tGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                           <  2:343954/100‑1 (MQ=255)

                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCa                          >  1:1306199/1‑100 (MQ=255)

                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCa                          >  2:2992342/1‑100 (MQ=255)

                                                                       cTTCCGCTTTTTCACAAAAGGAAATTCACTGATTTAGATGGGCAATATTATCTTTTAACTGAACGTTGATGCTCAAATAGCGATCGCGCATCTCCTCCa                          >  2:1251180/1‑99 (MQ=255)

                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                       >  1:445622/1‑100 (MQ=255)

                                                                         tCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtt                       >  1:1564186/1‑100 (MQ=255)

                                                                          ccGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttt                      <  1:309636/100‑1 (MQ=255)

                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                    >  2:287706/1‑100 (MQ=255)

                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                    >  2:442166/1‑100 (MQ=255)

                                                                            gCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGctttt                    >  2:1487167/1‑100 (MQ=255)

                                                                            gCTTTTTCACGAACGACAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttttt                    >  2:1885927/1‑100 (MQ=255)

                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                   <  2:2854723/100‑1 (MQ=255)

                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                   <  2:2558427/100‑1 (MQ=255)

                                                                              tttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGTACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGc                  <  2:318631/100‑1 (MQ=255)

                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc               >  2:1638672/1‑100 (MQ=255)

                                                                                   cACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAg             >  1:2423341/1‑100 (MQ=255)

                                                                                     cGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGcc           >  2:234140/1‑100 (MQ=255)

                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg        <  1:196007/100‑1 (MQ=255)

                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc      <  1:3296627/100‑1 (MQ=255)

                                                                                           gCAATTCCCTGATTTAGCTGGTCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACa     <  1:3244559/100‑1 (MQ=255)

                                                                                             aaTTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGa   >  1:1432363/1‑100 (MQ=255)

                                                                                              aTTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGAc  >  1:2974525/1‑100 (MQ=255)

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGAC  >  NC_000913/3352576‑3352769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`