Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,436,256 G→A 100% A350A (GCG→GCA rsmB → 16S rRNA m(5)C967 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,436,2560GA95.9% 506.8 / 6.0 168A350A (GCG→GCArsmB16S rRNA m(5)C967 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base A (67/93);  total (72/96)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

GCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAAT  >  NC_000913/3436159‑3436352
                                                                                                 |                                                                                                
gCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGcaca                                                                                                >  2:1469983/1‑100 (MQ=255)
gCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGcaca                                                                                                >  2:768850/1‑100 (MQ=255)
  aTTTTATTATATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAAt                                                                                              <  1:3860961/100‑1 (MQ=255)
  aTTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGGCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACGCGCACaa                                                                                               <  2:2253347/99‑1 (MQ=255)
   ttttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAAtt                                                                                             <  2:1893400/100‑1 (MQ=255)
   ttttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAAtt                                                                                             <  1:3799070/100‑1 (MQ=255)
   ttttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCAAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAAtt                                                                                             <  1:1518717/100‑1 (MQ=255)
    tttATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTg                                                                                            <  2:1058948/100‑1 (MQ=255)
        ttatgtGCTCCTTGTTCACCAACCCGTGTGCTTCGTCGCCAATCAGATATTAAACGGTTACGTCGCGATCGCGATATCCCGTAACTCGCACAATTGCAg                                                                                         <  2:228485/94‑1 (MQ=255)
        ttaGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAg                                                                                         >  1:777887/1‑99 (MQ=255)
           gATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTg                                                                                     >  1:141895/1‑100 (MQ=255)
           gATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTg                                                                                     >  1:3837975/1‑100 (MQ=255)
             tGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGAGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGaa                                                                                   >  1:3713376/1‑100 (MQ=255)
              gcgcCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGaaa                                                                                  <  2:2687144/100‑1 (MQ=255)
                gcCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAAtt                                                                                <  1:2509050/100‑1 (MQ=255)
                 ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtc                                                                               <  1:354661/100‑1 (MQ=255)
                 ccTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtc                                                                               >  2:696305/1‑100 (MQ=255)
                  cTTGTTCAGCGACCGGTGTGATTCGTAGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                              >  2:557302/1‑100 (MQ=255)
                  cTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAAtt                                                                                >  2:3448512/1‑98 (MQ=255)
                  cTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                              >  1:2580219/1‑100 (MQ=255)
                  attGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATtct                                                                              <  2:3767907/99‑1 (MQ=255)
                      ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTTCAGACTGCAAttgtgcga                                                                          <  2:1426435/100‑7 (MQ=255)
                      ttCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGAc                                                                          <  1:2306375/100‑1 (MQ=255)
                       tCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACg                                                                         >  2:1631993/1‑100 (MQ=255)
                        cAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCTATATCCCGGAACTCGCACAATTGAAGTCTGAAATTCTCGAc                                                                          >  1:785247/1‑98 (MQ=255)
                        cAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGc                                                                        <  2:127224/100‑1 (MQ=255)
                         aGCAACCGGTGTGATTCGTCGCCATCAAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGcc                                                                       >  2:1078849/1‑100 (MQ=255)
                            aaCCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCAtt                                                                    <  2:777703/100‑1 (MQ=255)
                              ccGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTg                                                                  <  1:3462455/100‑1 (MQ=255)
                               cGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTgg                                                                 >  1:3904139/1‑100 (MQ=255)
                                ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                                <  1:2969938/100‑1 (MQ=255)
                                ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                                <  2:1396524/100‑1 (MQ=255)
                                ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGc                                                                <  2:2304208/100‑1 (MQ=255)
                                ggTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGAGATCGCGATATCCCGGAACTCGTACAATTGCAGTATGAAATTATCGACGCCATTTgg                                                                 >  2:154636/1‑99 (MQ=255)
                                 gtgtGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGcc                                                               <  1:627291/100‑1 (MQ=255)
                                  tgtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCg                                                              <  2:672295/100‑1 (MQ=255)
                                  tgtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCg                                                              <  2:89416/100‑1 (MQ=255)
                                  tgtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCg                                                              <  1:3890437/100‑1 (MQ=255)
                                   gtgATTCGTCGCCATCCAGATATTAATTGGTTACGTCGCGATCGCGATATTCTGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGc                                                             <  1:36170/100‑1 (MQ=255)
                                   gtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGc                                                             >  2:1540320/1‑100 (MQ=255)
                                   gtgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGc                                                             <  2:2492743/100‑1 (MQ=255)
                                    tgATTCGTCGCCATCGAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCa                                                            <  1:3877584/100‑1 (MQ=255)
                                    tgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCa                                                            >  2:3843302/1‑100 (MQ=255)
                                    tgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCa                                                            <  1:2116968/100‑1 (MQ=255)
                                    tgATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTATCGACGCCATTTGGCCGCa                                                            >  1:965443/1‑100 (MQ=255)
                                     gATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAt                                                           >  1:1129444/1‑100 (MQ=255)
                                      aTTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCAtt                                                          >  1:2486764/1‑100 (MQ=255)
                                         cgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaa                                                       >  2:1701510/1‑100 (MQ=255)
                                         cgtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTa                                                        >  2:555352/1‑99 (MQ=255)
                                          gtcgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaa                                                      <  2:2831566/100‑1 (MQ=255)
                                            cgCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTaaaaa                                                    <  1:652559/100‑1 (MQ=255)
                                                aTCCAGATATTAAATGGTTACGTCGAGATCGCGATATCCCGGAACTCGCCCAATTGCAGTCTGAAATTATCGACGCCATTTGGCCGCATTTAAAAACCgg                                                >  2:1145065/1‑100 (MQ=255)
                                                 tCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAACAACCGGt                                               <  1:2046262/100‑1 (MQ=255)
                                                  ccAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTg                                              >  1:885177/1‑100 (MQ=255)
                                                   cAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTgg                                             >  1:1119518/1‑100 (MQ=255)
                                                     gATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGaa                                           >  1:1944776/1‑100 (MQ=255)
                                                         ttAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAActct                                       <  1:3351650/100‑1 (MQ=255)
                                                         ttAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAActct                                       >  2:2497119/1‑100 (MQ=255)
                                                         ttAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAActct                                       <  1:2800666/100‑1 (MQ=255)
                                                           aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTAGACGCCATTAGGCCGCATTTAAAAACTGGTGGAACTCTgg                                     >  1:2274907/1‑100 (MQ=255)
                                                           aaaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTgg                                     <  1:3098213/100‑1 (MQ=255)
                                                           aaaTGGTTACGTCGCGATCACGATATACCGGAACTCGCACAATTGCAGTATGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTgg                                     >  2:1343310/1‑100 (MQ=255)
                                                            aaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGt                                    >  2:2794771/1‑100 (MQ=255)
                                                            aaTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTAGCCGCATTTAAAAACCGGTGGAACTCTGGt                                    >  1:745632/1‑100 (MQ=255)
                                                             aTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTc                                   <  1:438533/100‑1 (MQ=255)
                                                             aTGGTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTc                                   <  1:2617016/100‑1 (MQ=255)
                                                                gTTACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTAt                                >  1:1458943/1‑100 (MQ=255)
                                                                 ttACGTCGCTATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATg                               <  1:2739933/100‑1 (MQ=255)
                                                                 ttACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATg                               <  1:120641/100‑1 (MQ=255)
                                                                  tACGTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGc                              >  1:3017001/1‑100 (MQ=255)
                                                                     gTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAc                           <  2:1232413/100‑1 (MQ=255)
                                                                     gTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAc                           >  2:1051003/1‑100 (MQ=255)
                                                                     gTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAc                           >  2:1934318/1‑100 (MQ=255)
                                                                     gTCGCGATCGCGATATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAc                           <  1:1718618/100‑1 (MQ=255)
                                                                      tcgcgatcgcgaTATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                          <  1:1784986/100‑1 (MQ=255)
                                                                      tcgcgatcgcgaTATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCAcc                          <  1:152126/100‑1 (MQ=255)
                                                                        gctcttccgatCTCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTg                        <  1:3506112/93‑1 (MQ=255)
                                                                        gcgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTg                        >  2:3237308/1‑100 (MQ=255)
                                                                         ctatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGt                       <  1:2427598/98‑1 (MQ=255)
                                                                         cgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGt                       >  1:2439494/1‑100 (MQ=255)
                                                                         cgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGt                       >  2:1891673/1‑100 (MQ=255)
                                                                         cgatcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGt                       >  2:3756809/1‑100 (MQ=255)
                                                                           atcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGtt                      <  1:1912493/99‑1 (MQ=255)
                                                                            tcgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCg                    <  2:2778537/100‑1 (MQ=255)
                                                                             cgcgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCgg                   <  1:2195981/100‑1 (MQ=255)
                                                                               cgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                 <  1:521224/100‑1 (MQ=255)
                                                                               cgatATCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                 <  1:3220609/100‑1 (MQ=255)
                                                                               cgatATCCCGGAACTCGCACAATTGCAGTCTGAAATGCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGgtg                 <  2:796210/100‑1 (MQ=255)
                                                                                    tCCCGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTAcc            <  1:1934334/100‑1 (MQ=255)
                                                                                     cccGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCg           >  1:13137/1‑100 (MQ=255)
                                                                                       cGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGa         <  2:437650/100‑1 (MQ=255)
                                                                                       cGGAACTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGa         <  1:274022/100‑1 (MQ=255)
                                                                                           aCTCGCACAATTGCAGTCTGATATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagag     <  1:2462111/100‑1 (MQ=255)
                                                                                           aCTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAagag     <  2:2988069/100‑1 (MQ=255)
                                                                                            cTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAgaga    >  2:3673662/1‑100 (MQ=255)
                                                                                            cTCGCACAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAgaga    <  1:2942418/100‑1 (MQ=255)
                                                                                             tCGCACAATTGCAGTCTGATATTCTGGACGACATTTGGCCGCATATAAAAACCGGTGGAACTCTGGTATATGCACAATGTTTGGTGTTACCGGaa        >  2:3351128/1‑95 (MQ=255)
                                                                                              cgcaCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAAt  >  2:1581252/1‑100 (MQ=255)
                                                                                              cgcaCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAAt  >  1:3329007/1‑100 (MQ=255)
                                                                                              cgcaCAATTGCAGTCTGAAATACTCGACGCCATTTGGACGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAAt  >  2:2143796/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATGGTTACGTCGCGATCGCGATATCCCGGAACTCGCGCAATTGCAGTCTGAAATTCTCGACGCCATTTGGCCGCATTTAAAAACCGGTGGAACTCTGGTCTATGCCACCTGTTCGGTGTTACCGGAAGAGAAT  >  NC_000913/3436159‑3436352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: