Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,089 A→G 17.1% intergenic (‑47/+12) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0890AG17.1% 482.7 / 90.1 222intergenic (‑47/+12)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base A (92/92);  new base G (18/20);  total (110/112)
Fisher's exact test for biased strand distribution p-value = 8.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01

Only 100 of 227 total aligned reads displayed.

GACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCG  >  NC_000913/4311990‑4312188
                                                                                                   |                                                                                                   
 acaacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCATATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAt                                                                                                    <  1:1217135/100‑1 (MQ=255)
   aacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACATGTAACTCCGCGATGGCGGAATTa                                                                                                  <  2:614101/100‑1 (MQ=255)
   aacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTa                                                                                                  <  2:691797/100‑1 (MQ=255)
    acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                                 >  2:438534/1‑100 (MQ=255)
    acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                                 >  2:1537818/1‑100 (MQ=255)
     caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  1:3545250/100‑1 (MQ=38)
     caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                >  2:1186257/1‑100 (MQ=255)
      aTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCt                                                                                               <  2:3393598/100‑1 (MQ=38)
      aTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCt                                                                                               >  1:2988125/1‑100 (MQ=255)
      aTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCCATCGCGGAGTTACCt                                                                                               <  2:858605/100‑1 (MQ=255)
       ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                              <  2:3514365/100‑2 (MQ=38)
       ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                              <  1:1910791/100‑2 (MQ=38)
        tAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgt                                                                                             <  2:472818/100‑3 (MQ=38)
        tAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgt                                                                                             <  1:2074927/100‑3 (MQ=38)
          aGCCCACGAGTGTGTCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtcc                                                                                           <  1:918219/100‑5 (MQ=25)
          aGCCCACGAGTGTGCCGCTGAAATATTTTATATTTTTTTTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                                            <  1:3253344/99‑4 (MQ=25)
          aGCCCACGAGTGTGCCGCTGAAATATATCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCttttt                                                                                           >  2:195222/1‑100 (MQ=255)
           gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          >  1:1822185/1‑100 (MQ=255)
           gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          <  1:2701075/100‑1 (MQ=255)
            cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                                         >  1:1636922/1‑100 (MQ=255)
             ccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCtt                                                                                        >  2:2215106/1‑100 (MQ=255)
              cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                                       <  2:1656660/100‑1 (MQ=255)
              cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                                       <  2:2135139/100‑1 (MQ=255)
              cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                                       <  2:1003767/100‑1 (MQ=255)
               aCGAGTGTGCCGTTGAAATATTTCAGATATTTATTCATAATAATTTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAt                                                                                      <  1:483119/100‑1 (MQ=255)
               aCGAGTGTGCCGCTTAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGTTGACAGGTAACTCCTCGATGGCGGAATTACCTTTTTCtt                                                                                        <  2:1833719/98‑1 (MQ=255)
                cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtcccctta                                                                                      <  2:655960/99‑10 (MQ=25)
                cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                     <  1:543630/100‑1 (MQ=255)
                  aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                                   <  1:2266446/100‑1 (MQ=255)
                  aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                                   <  1:39229/100‑1 (MQ=255)
                    tgtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAg                                                                                 <  2:1201871/100‑1 (MQ=255)
                     gtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCAGAATTACCTTTTTCTTATTTCAgc                                                                                >  1:2736291/1‑100 (MQ=255)
                        ccGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcg                                                                             >  2:2358706/1‑100 (MQ=255)
                        ccGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcg                                                                             >  2:3855600/1‑100 (MQ=255)
                         cGCTGAAATATCTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                            >  2:3105918/1‑100 (MQ=255)
                          gCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAttagatcggaag                                                                           >  2:2817107/1‑90 (MQ=255)
                             gAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTgg                                                                        <  1:1466557/100‑1 (MQ=255)
                               aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGTGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCATCGCGCCTGGTg                                                                      <  2:1372099/100‑1 (MQ=255)
                                  atTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCAATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgaga                                                                   >  1:25052/1‑100 (MQ=255)
                                   tttCAGATATTTATTCATAATAATGTTATCACGGTAAGGGGACAGGTAAATCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgag                                                                    >  2:2271368/1‑98 (MQ=255)
                                    ttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAGCTTTTTCTTATTTCAGCGCGCCTGGTGagaag                                                                 <  1:3720817/100‑1 (MQ=255)
                                     tCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaaga                                                                >  1:1029610/1‑100 (MQ=255)
                                            ttttttTATTATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcat                                                          <  2:2056366/99‑2 (MQ=255)
                                             ttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaaca                                                        >  2:2954081/1‑100 (MQ=255)
                                                 tCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                                    <  1:3629556/100‑1 (MQ=255)
                                                     aataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGcc                                                >  1:2009393/1‑100 (MQ=255)
                                                     aataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGcc                                                <  1:1707409/100‑1 (MQ=255)
                                                       taatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCgg                                              <  1:2950795/100‑1 (MQ=255)
                                                       taatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGATGAATTACTTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAAt                                                      >  2:1873506/1‑92 (MQ=255)
                                                        aatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGt                                             <  2:3511497/100‑1 (MQ=255)
                                                         atGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtt                                            <  1:2555406/100‑1 (MQ=255)
                                                          tGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAACGCGCCTGTTGAGAAGAAATCAACAATTACGCCGGttt                                           >  1:776109/1‑100 (MQ=255)
                                                            ttCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttttt                                         >  2:2317152/1‑100 (MQ=255)
                                                            ttCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttttt                                         <  1:3048154/100‑1 (MQ=255)
                                                             tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTGCAGCGCGCCTGGGGAGAAGAAATCAACAATTGCGCCGGtttttt                                        >  2:3125298/1‑100 (MQ=255)
                                                              ctcACGGTAATGGTTTAGTTATCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGATAACATCTCAACCATTTCGCCTGttttttt                                       <  2:3539910/100‑2 (MQ=255)
                                                              ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                       >  2:260102/1‑100 (MQ=255)
                                                               tcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                       <  1:594888/99‑1 (MQ=255)
                                                               tcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGc                                      >  1:1778458/1‑100 (MQ=255)
                                                                cACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCa                                     <  2:3451209/100‑1 (MQ=255)
                                                                cACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCa                                     >  2:3011046/1‑100 (MQ=255)
                                                                cACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAACGCGCCTGGTGAGAAGAAATCAACAATTACGCCGATTTTTTGCa                                     >  1:670816/1‑100 (MQ=255)
                                                                   ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatat                                  <  1:696609/100‑1 (MQ=255)
                                                                    gTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatt                                 <  1:1683340/100‑1 (MQ=255)
                                                                      aaGGGGACAGTTAACTCCTCGATGGAGGAATTACCTTTTTCTTATTTCAGCGCGCATGGTGAGAAGAAAACAACAATAGCGCCGGTTTTTTTCatatt                                 >  2:258461/1‑98 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTTGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  2:1220619/100‑1 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  2:3481205/100‑1 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  1:3120451/100‑1 (MQ=255)
                                                                       aagaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaat                              >  2:199559/8‑100 (MQ=37)
                                                                       aagaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaat                              >  2:3749701/8‑100 (MQ=25)
                                                                       aGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               >  1:2596970/1‑99 (MQ=255)
                                                                        ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             <  1:2816179/100‑1 (MQ=255)
                                                                        ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  2:1106860/1‑100 (MQ=255)
                                                                        agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  1:1928364/7‑100 (MQ=37)
                                                                        agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGCGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  1:2871454/7‑100 (MQ=25)
                                                                          ggAAAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatt                           <  2:532439/100‑1 (MQ=255)
                                                                            aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                         >  1:1508693/3‑100 (MQ=38)
                                                                             aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                        >  2:2765472/2‑100 (MQ=38)
                                                                             aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTAAGAAGAAAACAAAAATTACGCCGGTTTTTTGCATATTAATATTAGc                        >  1:2029670/2‑100 (MQ=25)
                                                                              aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                        >  2:1477593/1‑99 (MQ=38)
                                                                              aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATATCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatagta                          >  2:241232/1‑97 (MQ=38)
                                                                              aGGTAACTCCGCGATTTCTTTATTATCTTTTTCTTAGTTCAGCGTGCCTGGGGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  1:867481/92‑1 (MQ=255)
                                                                              aGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                       <  2:1711443/100‑1 (MQ=255)
                                                                               ggTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  2:743751/1‑100 (MQ=38)
                                                                               ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  1:3169948/1‑100 (MQ=255)
                                                                                gTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCTGTTTTTTGCATATTAATATTAGCTTg                     <  2:2207514/100‑1 (MQ=255)
                                                                                 tatcTCCGCGATTGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt                    <  2:3255525/97‑1 (MQ=255)
                                                                                 tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt                    >  2:3701946/1‑100 (MQ=255)
                                                                                 tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt                    <  1:2462268/100‑1 (MQ=255)
                                                                                 tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt                    <  1:1426960/100‑1 (MQ=255)
                                                                                    cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCa                 >  2:3030838/1‑100 (MQ=255)
                                                                                      ccGCGATGGCGGAATTACCTTTTTCTTATTTCTGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCaa                <  1:1193312/99‑1 (MQ=255)
                                                                                      ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt               >  1:3750659/1‑100 (MQ=255)
                                                                                       cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGAATATTAATATTAGCTTGTTCAAt               >  1:2342818/1‑99 (MQ=255)
                                                                                          gATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg           <  2:1317354/100‑1 (MQ=255)
                                                                                             ggCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGc        <  1:2176600/100‑1 (MQ=255)
                                                                                              gCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGACGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCt       >  1:1129074/1‑100 (MQ=255)
                                                                                               cGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTg      >  2:253916/1‑100 (MQ=255)
                                                                                                  aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc   <  2:2488279/100‑1 (MQ=255)
                                                                                                  aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc   <  2:1918735/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCG  >  NC_000913/4311990‑4312188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: