Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,397,557 G→T 100% G49V (GGT→GTT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,5570GT98.9% 611.6 / ‑1.9 191G49V (GGT→GTT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (96/91);  total (99/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

Only 100 of 191 total aligned reads displayed.

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652
                                                                                                  |                                                                                               
  tgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTggg                                                                                             <  1:3922852/100‑1 (MQ=255)
  tgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTggg                                                                                             <  2:3773945/100‑1 (MQ=255)
  tgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTACGACACGTATCGATATTGATATCGAACGCGTTggg                                                                                             >  1:1599185/1‑100 (MQ=255)
    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGc                                                                                           <  1:682357/100‑1 (MQ=255)
     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCg                                                                                          >  1:1993938/1‑100 (MQ=255)
       tggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaa                                                                                        <  2:1700411/100‑1 (MQ=255)
       tggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaa                                                                                        <  1:1948998/100‑1 (MQ=255)
        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaaa                                                                                       >  2:115988/1‑100 (MQ=255)
        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGAGTATAGATATTGATATCAAACGCGTTGGGACGaaa                                                                                       >  1:1735151/1‑100 (MQ=255)
         gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAAc                                                                                      <  2:3863659/100‑1 (MQ=255)
         gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGCGCGAAAc                                                                                      <  1:2050501/100‑1 (MQ=255)
          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACt                                                                                     <  1:2906094/100‑1 (MQ=255)
          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACt                                                                                     >  1:2691378/1‑100 (MQ=255)
          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACt                                                                                     <  1:2618226/100‑1 (MQ=255)
          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACt                                                                                     >  1:2538987/1‑100 (MQ=255)
           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACtt                                                                                    >  1:1002192/1‑100 (MQ=255)
            gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAAc                                                                                      <  2:589756/97‑1 (MQ=255)
            gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGAGTATCGATATTGATATCGAAAGAGTTGGAGCGAAACtt                                                                                    >  1:2323823/1‑99 (MQ=255)
              gCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATc                                                                                 <  1:2129795/100‑1 (MQ=255)
               cGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATcc                                                                                <  2:1376466/100‑1 (MQ=255)
               cGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATcc                                                                                <  1:1437838/100‑1 (MQ=255)
                 aCCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGc                                                                              >  2:3340688/1‑100 (MQ=255)
                   cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTTATATCGAACGCGTTGTGACGAAACTTATCCGc                                                                              >  2:813475/1‑98 (MQ=255)
                   cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCAt                                                                            >  2:3777597/1‑100 (MQ=255)
                   cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCAt                                                                            >  2:2434352/1‑100 (MQ=255)
                    tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCAtt                                                                           >  2:232698/1‑100 (MQ=255)
                       gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACTCGTATCGATATTGATATAGAACGCGAAAGGGCGAAACTTATCCGCATACGt                                                                        >  1:1248971/1‑100 (MQ=255)
                       gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGCTATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGt                                                                        <  2:2088325/100‑1 (MQ=255)
                         cGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGa                                                                      >  2:3795048/1‑100 (MQ=255)
                            tAGTCAAAGAACTAGTTTAAAACTGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGTGCTATTCTTATCCGCATTCGTGATaa                                                                   <  2:2583123/100‑1 (MQ=255)
                              gTCAAAGAACTAGTTGAAAATAGTCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 <  1:617024/100‑1 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:2862089/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               <  1:2186318/100‑1 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:1787734/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:1102643/1‑100 (MQ=255)
                                  aaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTg                                                             <  1:725509/100‑1 (MQ=255)
                                   aGAACTAGTGGAAACCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTAGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                            >  1:1516320/1‑100 (MQ=255)
                                   aGAACCAGTTGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACTCGTTGGGGCAAAACTTATCCGCATTCGTGACAACGGc                                                               <  2:1153947/97‑1 (MQ=255)
                                    gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCt                                                              >  2:3340392/1‑97 (MQ=255)
                                    gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCg                                                           <  2:1614010/100‑1 (MQ=255)
                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  1:1304754/100‑1 (MQ=255)
                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  2:2965385/100‑1 (MQ=255)
                                      aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                         >  1:3584821/1‑100 (MQ=255)
                                       cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        >  1:1061864/1‑100 (MQ=255)
                                       cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        >  1:1596438/1‑100 (MQ=255)
                                         aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      <  2:1200913/100‑1 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  2:3284560/1‑100 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     <  1:3211525/100‑1 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  2:2013553/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGACGAAACTTATCCACATTAGTGATAACGACTGCGGTATCaaa                                                   >  1:1591734/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATAGTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTagatcg                                                   >  2:2408141/1‑95 (MQ=255)
                                              aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 >  2:570826/1‑100 (MQ=255)
                                                aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                >  2:984156/1‑99 (MQ=255)
                                                aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                               <  1:291520/100‑1 (MQ=255)
                                                 aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATTTCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                              <  2:502078/100‑1 (MQ=255)
                                                 aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                              <  2:2198610/100‑1 (MQ=255)
                                                   cgCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACAGCTGCGGTATCAAAAAAGATg                                            >  1:1330874/2‑100 (MQ=255)
                                                      cgcGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAg                                          <  2:3908273/97‑1 (MQ=255)
                                                       tCGATGCAGGTGGGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgct                                        <  2:3121366/100‑1 (MQ=255)
                                                       tCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAAATTATCAGCATTCGTGATAACGACTCCGGTATCAAAAAAGATGAtct                                        >  2:1063481/1‑100 (MQ=255)
                                                           tGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                    >  2:3618317/1‑100 (MQ=255)
                                                           tGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                    >  1:2152055/1‑100 (MQ=255)
                                                           tGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                    <  1:2721434/100‑1 (MQ=255)
                                                            gCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgc                                   >  2:1127100/1‑100 (MQ=255)
                                                            gCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgc                                   >  2:249623/1‑100 (MQ=255)
                                                             cAGGTGCGACGCGTATCGATATTGATATCTAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  <  2:662333/100‑1 (MQ=255)
                                                             cAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  >  1:873408/1‑100 (MQ=255)
                                                             cAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  >  1:3863747/1‑100 (MQ=255)
                                                               tccgatctgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                <  2:630066/92‑1 (MQ=255)
                                                               ggTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                >  1:3179431/1‑100 (MQ=255)
                                                                ccccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  2:598434/6‑100 (MQ=25)
                                                                ccccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  1:209841/6‑100 (MQ=25)
                                                                 tGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTAGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                              >  1:274698/1‑100 (MQ=255)
                                                                  ccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                             >  1:2248107/4‑100 (MQ=37)
                                                                  ccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                             >  1:204207/4‑100 (MQ=37)
                                                                    gACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  1:1353526/100‑1 (MQ=255)
                                                                    gACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  2:873397/100‑1 (MQ=255)
                                                                    gACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  2:1322063/100‑1 (MQ=255)
                                                                     aCGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  2:859042/99‑1 (MQ=255)
                                                                      cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  2:1019828/100‑1 (MQ=255)
                                                                      cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  2:3690679/1‑100 (MQ=255)
                                                                       gcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                        <  1:1476398/100‑1 (MQ=255)
                                                                       gcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                        <  1:3099678/100‑1 (MQ=255)
                                                                        gcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                        >  2:2799289/5‑100 (MQ=38)
                                                                        cgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       >  2:845347/1‑100 (MQ=255)
                                                                         gTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCg                      <  2:1954266/100‑1 (MQ=255)
                                                                            tCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGAAATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   >  1:2459181/1‑100 (MQ=255)
                                                                             cGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCTCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt                  <  1:33490/100‑1 (MQ=255)
                                                                             cGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGATCTGGCGCTGGCGCTGGCTCGTCa                   <  2:3394453/99‑1 (MQ=255)
                                                                              gATATTGATATCGAACGCGTTGGGGTTAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 <  1:2274188/100‑1 (MQ=255)
                                                                              gATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 <  1:2558283/100‑1 (MQ=255)
                                                                              gATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 >  1:3616140/1‑100 (MQ=255)
                                                                                tatTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               >  2:969158/1‑100 (MQ=255)
                                                                                  ttGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             <  1:2025023/100‑1 (MQ=255)
                                                                                  ttGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             <  2:3486953/100‑1 (MQ=255)
                                                                                    gATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacca           >  1:420131/1‑100 (MQ=255)
                                                                                       atCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa        <  2:2225521/100‑1 (MQ=255)
                                                                                       atCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa        <  1:2504519/100‑1 (MQ=255)
                                                                                         cGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa      >  1:2225816/1‑100 (MQ=255)
                                                                                         cGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa      >  1:63352/1‑100 (MQ=255)
                                                                                                  |                                                                                               
GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: