BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	464,356	A→T	15.2%	intergenic (+48/‑46)	ybaV → / → fadM	putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	464,356	0	A	T	15.2%	453.2 / 62.1	169	intergenic (+48/‑46)	ybaV/fadM	putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
Reads supporting (aligned to +/- strand): ref base A (67/76); new base T (8/18); total (75/94)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01

Only 100 of 172 total aligned reads displayed.

GGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTC  >  NC_000913/464257‑464455

  ggATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAg                                                                                                    >  1:3713378/1‑99 (MQ=255)

  ggATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGt                                                                                                   >  1:2764373/1‑100 (MQ=255)

    aTGGGCAATTCGCTGGTGGAACGTAATATGGTAGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTa                                                                                                 <  1:1773588/100‑1 (MQ=255)

     tGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                                                                >  2:2443467/1‑100 (MQ=255)

       ggCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                                                                >  2:1247308/1‑98 (MQ=255)

       ggCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGa                                                                                              >  2:1670066/1‑100 (MQ=255)

       ggCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGa                                                                                              >  1:1274364/1‑100 (MQ=255)

        gCAATTCGTTGGTGTAACTTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATg                                                                                               <  1:809111/98‑1 (MQ=255)

        gCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAc                                                                                             <  1:3856238/100‑1 (MQ=255)

         cAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                            >  2:3390769/1‑100 (MQ=255)

         cAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                            <  2:3883154/100‑1 (MQ=255)

         cAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                            >  2:948923/1‑100 (MQ=255)

          aaTTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACct                                                                                           <  2:2950332/100‑1 (MQ=255)

           aTTCGCTGGTTGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTTCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctc                                                                                          <  1:1981441/100‑1 (MQ=255)

           aTTCGCTGGTGGAACTTAATCTTGTGGTATTAATTCTTTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctc                                                                                          <  1:2048667/100‑1 (MQ=255)

            ttCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctct                                                                                         <  2:423483/100‑1 (MQ=255)

            ttCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctct                                                                                         <  1:2984956/100‑1 (MQ=255)

              cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGTCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttc                                                                                       <  1:1010504/100‑3 (MQ=38)

              cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttc                                                                                       <  2:1934048/100‑3 (MQ=39)

              cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttc                                                                                       <  1:794778/100‑3 (MQ=39)

              cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttc                                                                                       <  1:3294789/100‑3 (MQ=38)

              cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTAGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGt                                                                                       >  1:1077827/1‑100 (MQ=255)

               gCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTa                                                                                      <  2:2030625/100‑1 (MQ=255)

                cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                     <  1:2807409/100‑1 (MQ=255)

                cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttcag                                                                                     <  1:3781222/100‑5 (MQ=38)

                 tggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACt                                                                                    <  2:3332317/100‑1 (MQ=255)

                 tggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACt                                                                                    <  1:3801898/100‑1 (MQ=255)

                  ggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACtt                                                                                   >  1:2616951/1‑100 (MQ=255)

                  ggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACtt                                                                                   >  1:3825096/1‑100 (MQ=255)

                   gtggAACGTAATCTGGCGGTATTAACCCTGTAATTTATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttcagtct                                                                                  <  1:1848104/100‑8 (MQ=37)

                   gtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTta                                                                                  <  2:186057/100‑1 (MQ=255)

                    tggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtat                                                                                 <  1:1145926/100‑1 (MQ=255)

                    tggAACGTAATCTGGCGGTATTAACCATGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtat                                                                                 >  1:2374747/1‑100 (MQ=255)

                     ggAACGTAATCTGGCGGTATTAACTCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtata                                                                                <  1:2513518/100‑1 (MQ=255)

                     ggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtata                                                                                >  1:1349307/1‑100 (MQ=255)

                      gAACGTAATCTGGCTGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaa                                                                               <  1:595365/100‑1 (MQ=255)

                         cGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATGGTGGCAATTTTTTGCCAGCATGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaa                                                                            >  2:2742994/1‑100 (MQ=255)

                          gTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaac                                                                           <  2:2977357/100‑1 (MQ=255)

                           tAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaaca                                                                          >  1:3359520/1‑100 (MQ=255)

                            aaTCTCGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaa                                                                         >  2:1174023/1‑100 (MQ=255)

                              tCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACg                                                                       >  2:3882287/1‑100 (MQ=255)

                                  gCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAg                                                                   >  1:723134/1‑100 (MQ=255)

                                    ggTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGt                                                                 <  2:2247151/100‑1 (MQ=255)

                                          aaCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgc                                                           >  2:1408646/1‑100 (MQ=255)

                                          aaCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgc                                                           >  1:2231800/1‑100 (MQ=255)

                                           aCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcg                                                          >  1:3169984/1‑100 (MQ=255)

                                           aCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcg                                                          >  1:407089/1‑100 (MQ=255)

                                            cccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCATTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgc                                                           >  1:2094975/1‑98 (MQ=255)

                                              cTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTa                                                       <  1:2630822/100‑1 (MQ=255)

                                               tGTAATTATTTTGCATAGTGTCAATTTTTTGCCAGACTGAAGATGTCATACCAGTTATGGCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTAt                                                      <  1:1782248/100‑1 (MQ=255)

                                                gTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                     >  1:856197/1‑100 (MQ=255)

                                                gTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                     >  2:1000599/1‑100 (MQ=255)

                                                 taattaatTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACAGTTATGAACTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGc                                                    <  2:1080666/100‑1 (MQ=255)

                                                 taattaatTTGCATAGTGGCAATTTTTTGCCAGACTAAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                     <  2:223245/99‑1 (MQ=255)

                                                    ttaattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCaaa                                                 <  1:2024031/100‑1 (MQ=255)

                                                     taattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAac                                                >  1:1724985/1‑100 (MQ=255)

                                                       attTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAacac                                              <  1:2952192/100‑1 (MQ=255)

                                                              tAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCaa                                       <  1:1604250/100‑1 (MQ=255)

                                                                  ggcaaaaaattGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGtt                                   >  2:848961/10‑100 (MQ=255)

                                                                   gCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGTAAACACAAATCAAAGTTc                                  >  2:3189759/1‑100 (MQ=255)

                                                                     aaTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGt                                >  2:1659012/1‑100 (MQ=255)

                                                                      aTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTg                               <  2:615806/100‑1 (MQ=255)

                                                                      aTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTg                               <  1:974763/100‑1 (MQ=255)

                                                                       ttttttGCCAGACTGAAGAGTTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTgg                              <  1:2327262/100‑1 (MQ=255)

                                                                        tttttGCCAGACTGAAGAGGTCATACAAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGATATACAAACACAAATCAAAGTTCGTgg                              >  2:1445775/1‑99 (MQ=255)

                                                                          tttGCCAGACTGAAGAGGTCATACCAGTTATGTCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGata                           <  1:2722256/100‑1 (MQ=255)

                                                                           ttGCCAGACTGAAGAGGTTATACTAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGatat                          <  1:3541024/100‑1 (MQ=255)

                                                                            tgacAGACTGAAGAGGTCATACCAGTGATGAGCTCTGTACTTATAACAACAACGTAAAGTTATTGCCCTATGCAAACACGAATCAAAGTTCGTGGatat                          <  2:3562097/96‑1 (MQ=255)

                                                                             gCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatca                        >  1:2248437/1‑100 (MQ=255)

                                                                             gCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatca                        >  2:3245310/1‑100 (MQ=255)

                                                                               cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcatc                      >  2:443020/1‑100 (MQ=255)

                                                                               cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcatc                      >  2:3123104/1‑100 (MQ=255)

                                                                               cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcatc                      <  1:446230/100‑1 (MQ=255)

                                                                               cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcat                       >  1:3608433/1‑99 (MQ=255)

                                                                                aGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCAtct                     <  1:2474539/100‑1 (MQ=255)

                                                                                    tacagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCACTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCg                   >  2:258267/4‑98 (MQ=38)

                                                                                    tGAAGAGGTCATATCAGTTATGACCTCTGTACTTATAATAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGAc                 <  1:3330270/100‑1 (MQ=255)

                                                                                    tGAAGAGGTCATACCAGTTACGACCTCTGTACTTATACCAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCTAATTTCGTGGATATCATCTCGAc                 <  2:567760/100‑1 (MQ=255)

                                                                                     gAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGAc                 >  1:872466/1‑99 (MQ=255)

                                                                                     acagagGTCATAACTGGTATGACCTCTGTACTAATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGAc                 >  1:3728774/3‑99 (MQ=38)

                                                                                      aaGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGt               <  2:2284737/100‑1 (MQ=255)

                                                                                       agagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGtt              <  2:2794927/100‑1 (MQ=255)

                                                                                        gagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGttt             <  2:2433831/100‑1 (MQ=255)

                                                                                        gagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGctt             >  2:1846570/1‑100 (MQ=255)

                                                                                        gagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGttt             >  1:2487196/1‑100 (MQ=255)

                                                                                           ntCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAcc          >  2:2347485/2‑100 (MQ=255)

                                                                                           gTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAcc          <  1:2780425/100‑1 (MQ=255)

                                                                                           gTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGATATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAcc          <  1:1306797/100‑1 (MQ=255)

                                                                                            tCATACCAGTTATGATCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCa         <  2:395701/100‑1 (MQ=255)

                                                                                            tCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCa         >  1:1453367/1‑100 (MQ=255)

                                                                                             cATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGAAGTTTACCa         >  2:3757581/1‑99 (MQ=255)

                                                                                              aTACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGc       <  2:1217238/100‑1 (MQ=255)

                                                                                               tACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCa      <  1:2603053/100‑1 (MQ=255)

                                                                                               tACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCa      <  2:7633/100‑1 (MQ=255)

                                                                                               tACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTAACCAGCa      >  1:1546640/1‑100 (MQ=255)

                                                                                               tACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTAGATATCATCTCGACGTTTACCAGCa      >  1:539793/1‑100 (MQ=255)

                                                                                                aCCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCAc     >  2:1199934/1‑100 (MQ=255)

                                                                                                 ccAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCTTGGATATCATCTCGACGTTTACCAGCACg    <  2:596888/100‑1 (MQ=255)

                                                                                                 ccAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACg    >  1:444199/1‑100 (MQ=255)

                                                                                                   aGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTc  <  2:2366005/100‑1 (MQ=255)

GGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTC  >  NC_000913/464257‑464455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`