Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 674,567 C→T 19.8% V73I (GTC→ATC)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913674,5670CT19.8% 303.6 / 61.0 141V73I (GTC→ATC) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (51/62);  new base T (10/18);  total (61/80)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

Only 100 of 143 total aligned reads displayed.

CGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAT  >  NC_000913/674468‑674666
                                                                                                   |                                                                                                   
cgATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGAc                                                                                                     >  1:2992131/1‑100 (MQ=255)
   tgttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGtt                                                                                                  >  2:3875626/1‑100 (MQ=255)
      tgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAATGCGTCCCAGCCGATCGGCTGCAGGATGttttt                                                                                               >  2:508924/1‑100 (MQ=255)
      tgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGttttt                                                                                               >  1:729075/1‑100 (MQ=255)
       gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTg                                                                                              <  2:78232/100‑1 (MQ=255)
       gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTg                                                                                              >  2:3795193/1‑100 (MQ=255)
       gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTg                                                                                              <  1:2713080/100‑1 (MQ=255)
       gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTg                                                                                              <  1:2087375/100‑1 (MQ=255)
         cGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGcc                                                                                            >  1:1104709/1‑100 (MQ=255)
         cGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGcc                                                                                            <  1:849919/100‑1 (MQ=255)
           tACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCa                                                                                          <  1:3344365/100‑1 (MQ=255)
             cGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGc                                                                                        <  2:266288/100‑1 (MQ=255)
             cGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACAAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGc                                                                                        >  1:2069335/1‑100 (MQ=255)
              gTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCa                                                                                       >  2:2012870/1‑100 (MQ=255)
              gTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCa                                                                                       >  1:3670675/1‑100 (MQ=255)
                 cACGGTGCCGGAGCGGTGTTGTATTTCTCCGCCGCGCCTTCCGCAGGCAGTCCAAACGCGTCCCCGCCGATCGGCTTCAGGACGTTTTTGCCCAGc                                                                                        <  2:912427/96‑1 (MQ=255)
                    ggTGCCGGAGCGGTGTTGTTTTTCTCCGCCGCGCCTTCCGTAGGCAGACCAAACGCGTCCCAGCCTATCGGCTGCATGATGTTTTTGCCCAGCATACGCt                                                                                 <  2:265769/100‑1 (MQ=255)
                     gTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTg                                                                                <  1:2476200/100‑1 (MQ=255)
                      tGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGTCGTTTTTGCCCAGCATACGCTgg                                                                               <  2:755878/100‑1 (MQ=255)
                       gCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGt                                                                              >  1:591748/1‑100 (MQ=255)
                        ccGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTa                                                                             >  1:2052607/1‑100 (MQ=255)
                         cGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCGTATGCTgggca                                                                            <  2:3173188/100‑4 (MQ=255)
                           gAGCGGTGTTGTTTTTCACCGTCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCGTATGCTgggcaaa                                                                          <  1:2974371/100‑6 (MQ=255)
                           gAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCGTATGCTgggcaaa                                                                          <  2:3762013/100‑6 (MQ=255)
                           gAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCg                                                                          <  1:3028940/100‑1 (MQ=255)
                           gAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCg                                                                          >  1:2786480/1‑100 (MQ=255)
                             gCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCggg                                                                        >  1:3445583/1‑100 (MQ=255)
                               ggTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCg                                                                      >  1:1790933/1‑100 (MQ=255)
                               ggTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCg                                                                      >  1:26657/1‑100 (MQ=255)
                                gtgtTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACTCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGa                                                                     <  2:732455/100‑1 (MQ=255)
                                gtgtTGTTTTTCACCGCCGCGCCTTACGCAGGCAGACCAAACGCGTCTCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCg                                                                          <  2:1124390/95‑1 (MQ=255)
                                 tgttgtTTTTCACCGCCGCGCCTTCTTCATGCAGTCCAAACGCGTCCCAGCCGATCGGCTGCAGGACTTTTTTGCCCAGCATACGCTGGTAGCGGGc                                                                       <  2:1024666/97‑1 (MQ=255)
                                 tgttgtTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                    <  1:3187737/100‑1 (MQ=255)
                                 tgttgtTTTTCACCGCCGCGCCCTCCGCAGGAAGACCGAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                    <  2:2635847/100‑1 (MQ=255)
                                  gtttttTTTTACCGCCGGGCCTTCCGCAGGCAGACCAAACGCGTCCCACGCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                   <  1:2519004/100‑1 (MQ=255)
                                  gttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                   <  2:1976971/100‑1 (MQ=255)
                                  gttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                   >  1:3895183/1‑100 (MQ=255)
                                   ttgtccttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCCATCGGCTGCAGGATGATGTTGCCCAGCATACGCTGGTAGCGGGCGATCa                                                                  <  2:1329848/94‑1 (MQ=255)
                                       ttttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                              >  1:2771948/1‑100 (MQ=255)
                                       ttttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                              <  1:809340/100‑1 (MQ=255)
                                       ttttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                              >  2:105382/1‑100 (MQ=255)
                                        tttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCa                                                             >  2:699338/1‑100 (MQ=255)
                                         ttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAc                                                            <  1:1734824/100‑1 (MQ=255)
                                         ttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAc                                                            <  2:1970168/100‑1 (MQ=255)
                                          tCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                           <  1:2229169/100‑1 (MQ=255)
                                           cACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCg                                                          <  1:2940490/100‑1 (MQ=255)
                                            aCCGCCGCGCCTTTCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGa                                                         <  1:722918/100‑1 (MQ=255)
                                               gccgcGCCTTCCGCAGGCAGTCCAAACGCGTCCCAGCCGATCGACTGCAGGATGTTTTTGCCCAGCATACCCTGGTAGCAGGCGATCACGTCAcc                                                           >  2:2386379/1‑95 (MQ=255)
                                               gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                      >  1:1582900/1‑100 (MQ=255)
                                               gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                      <  2:2069316/100‑1 (MQ=255)
                                               gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                      >  1:655988/1‑100 (MQ=255)
                                               gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                      <  2:3895144/100‑1 (MQ=255)
                                               gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTGGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                      <  2:509330/100‑1 (MQ=255)
                                                ccgcGTCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgt                                                     <  1:1471754/100‑1 (MQ=255)
                                                    gcCTTCCGCAGGCAGACCAAACGCGACCCCGCCGATCGGCTGCCGGACGTTTTTGCCCAGCACACGCTGGTAGCGGCCGATCACGTCACCGATGGTGTAg                                                 <  2:2071744/100‑1 (MQ=255)
                                                      cTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                               >  2:213336/1‑100 (MQ=255)
                                                      cTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                               >  2:3477117/1‑100 (MQ=255)
                                                       ttCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTta                                              <  1:3287395/100‑1 (MQ=255)
                                                         ccGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacg                                            >  2:2853724/1‑100 (MQ=255)
                                                           gcaggcagACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgta                                          <  2:3910388/100‑1 (MQ=255)
                                                           gcaggcagACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgta                                          >  1:2116905/1‑100 (MQ=255)
                                                            cagtcagACCTTACGTGTCCCAGTCGACCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGTGCGATCACGTCACCGATGGTGTAGTtacgta                                          <  2:2829386/99‑1 (MQ=255)
                                                            caggcagACCAAACGCGTCCCAGCCGAGCGGCTGCAGGACGTGTTTGCCCAGCATTCGCTGGTAGCGTGCGATCACGTCACCGATGGTGTAGTtacgta                                          <  2:1183146/99‑1 (MQ=255)
                                                              ggcagACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTacgtacgt                                       >  2:466225/1‑100 (MQ=255)
                                                                 agACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGc                                    <  1:1265446/100‑1 (MQ=255)
                                                                    ccAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCa                                 >  1:2263441/1‑100 (MQ=255)
                                                                     cAAACGCGTCCCAGCCGATTGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAt                                <  1:3687395/100‑1 (MQ=255)
                                                                     cAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTAGTAGCGGGCAATAACGTCACCGAGGGTGTAGTTACGTAAGTGGCCCAt                                >  1:1141013/1‑100 (MQ=255)
                                                                      aaaCGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtn                               <  2:2241225/100‑2 (MQ=255)
                                                                      aaaCGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAt                                <  1:762333/99‑1 (MQ=255)
                                                                       naCGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGGGACCCAtg                               >  1:1718223/2‑99 (MQ=255)
                                                                         cgcgTCCCAGCCGATCGGTTGCAGGACGTTTTTGTCCAGCATTCGTTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATgtgt                            <  1:3102835/100‑1 (MQ=255)
                                                                          gtcctgcAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTa                           >  1:1442325/7‑100 (MQ=255)
                                                                          gcgTCCCAGCCGAGCGGCTGCAGGATGTTTTTGCCCAGCATACGTTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTa                           <  2:1015033/100‑1 (MQ=255)
                                                                            gTCCCAGCCGATCGGCTGCAGGACGTTTTTTTTTTTTTTACTCTTGTAGCGGGCGATCACGTCAACGATGGTGTAGTTACGTACGTGGCCAATGTGTatt                         >  2:3680652/1‑98 (MQ=255)
                                                                             tCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGCAGTTACGTACGTGGCCCATGTGTAGTc                        >  1:2295695/1‑100 (MQ=255)
                                                                              cccAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                       >  1:2487103/1‑100 (MQ=255)
                                                                              cccAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTAGCCCATGTGTAGTCg                       >  2:1445618/1‑100 (MQ=255)
                                                                              cccAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGAAGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                       >  1:1960345/1‑100 (MQ=255)
                                                                               ccAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGa                      >  1:1709095/1‑100 (MQ=255)
                                                                                cAGCCGCTCGGCTGAAGGACTTTTTTGCCAAGCATACGCTGGTAGCGGGCGATCACATCACCGATGGTGTAGTTACGTACGTAGCCCATGTGTAGta                        <  2:1321885/97‑2 (MQ=255)
                                                                                cAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                     <  1:130913/100‑1 (MQ=255)
                                                                                cAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                     >  2:2854671/1‑100 (MQ=255)
                                                                                  gCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCa                   <  2:934391/100‑1 (MQ=255)
                                                                                   cttccgatcTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAg                  <  1:1021088/92‑1 (MQ=255)
                                                                                      aTCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAg               <  1:3915202/100‑1 (MQ=255)
                                                                                        cGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCTATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGa             <  2:1199001/100‑1 (MQ=255)
                                                                                         ggCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGAt            >  2:2628114/1‑100 (MQ=255)
                                                                                         ggCTGCAGCACGTTTTTGCCAAGTATTCGTTTGTAGCGGGCGATCACGTCACCGCTTGTGTAGTTTCGTTCGTGGTCCATGTGTAGTCGACCAGAAg               <  2:1710562/97‑1 (MQ=255)
                                                                                              cAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTATGTACGTGGGCCATGTGTAGTCGACCAGAAGGATAGGGa       <  2:2403965/100‑1 (MQ=255)
                                                                                              cAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGa       <  1:618035/100‑1 (MQ=255)
                                                                                               aGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGaa      >  1:1876135/1‑100 (MQ=255)
                                                                                               aGGACGTTTTTGCCCAAAATACGCTGGTAGCGAGCGATCACGTAACCGATGGTGTAGTTACATACGTGGCCCATGTGTAGTAGACCAGAAGGATAGGGaa      >  2:1536088/1‑100 (MQ=255)
                                                                                                tgACGTTTTTGCCCAGCATACGCTGGGAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCAATGTGTAGTCGACCAGAAGGATAGGGaa      >  2:1695384/2‑99 (MQ=255)
                                                                                                ggATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATAACGTCACCGATGGTGTAGTTACGTACGTGGCCGAGGTGTAGTAGACCAGAAGGATAGGGGAg     >  2:2927626/1‑100 (MQ=255)
                                                                                                ggACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAg     >  1:947830/1‑100 (MQ=255)
                                                                                                ggACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAg     >  1:3236588/1‑100 (MQ=255)
                                                                                                 gACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGc    <  2:3901477/100‑1 (MQ=255)
                                                                                                  aCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCa   <  2:2116886/100‑1 (MQ=255)
                                                                                                   cGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt  >  2:3460182/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAT  >  NC_000913/674468‑674666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑