Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 111,013 G→A 15.8% intergenic (+29/‑31) secA → / → mutT preprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913111,0130GA15.8% 434.6 / 56.2 187intergenic (+29/‑31)secA/mutTpreprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (85/70);  new base A (12/18);  total (98/89)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 190 total aligned reads displayed.

GACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCG  >  NC_000913/110917‑111111
                                                                                                |                                                                                                  
gACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGAtt                                                                                                 >  1:895869/1‑100 (MQ=255)
  cGTAACGATCCTTGCCCGGGCTGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAAtcc                                                                                                <  2:3351274/99‑3 (MQ=39)
   gTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTg                                                                                              <  1:621158/100‑1 (MQ=255)
    tAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTTCCATGGCCGCCTGCAATAAAAGCTAACTATTGAAGTAAAAAGCGCAGGATTCTg                                                                                              >  1:197089/1‑99 (MQ=255)
    tAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTgc                                                                                             >  1:2440899/1‑100 (MQ=255)
     aaCGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACAGTTGAAGTAAAAGGCGCAGGATTCTgcg                                                                                            >  1:277882/1‑100 (MQ=255)
       cGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGcc                                                                                          <  2:1002701/100‑1 (MQ=255)
         aTCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCtt                                                                                        <  2:675427/100‑1 (MQ=255)
         aTCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCt                                                                                         <  1:1930348/99‑1 (MQ=255)
          tCCTTGCCCTTGTGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCtt                                                                                        <  1:3847823/99‑1 (MQ=255)
          tCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCttt                                                                                       <  1:1144410/100‑1 (MQ=255)
           ccTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCttt                                                                                       <  2:1565483/99‑1 (MQ=255)
            cTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCttttt                                                                                     >  2:2306020/1‑100 (MQ=255)
            cTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCttttt                                                                                     <  2:2440876/100‑1 (MQ=255)
            cTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCttttt                                                                                     <  2:1640094/100‑1 (MQ=255)
            cTTGCCCGAGCGGAACTAGTAACAACTACAAGAAGTGCCATGGCCGCCTGCAATAACAGCTAACTGTTGCAGTAAAAGGCGCAGGATTCTGCGCGttttt                                                                                     <  2:319882/100‑1 (MQ=255)
              tGCCCGTGCGGTTCTGGTAAAAAATACAAGCTGTGTCATGTCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCttttact                                                                                   <  1:1102471/100‑4 (MQ=37)
              tGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCttttact                                                                                   <  2:2761162/100‑4 (MQ=39)
              tGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCttttact                                                                                   <  2:1394985/100‑4 (MQ=255)
               gCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATTGTTGTCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGAATCCTGCGCCttttactt                                                                                  <  1:102356/100‑5 (MQ=37)
               gCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTta                                                                                   >  2:1861588/1‑99 (MQ=255)
                cccGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTtata                                                                                 >  2:2002914/1‑100 (MQ=255)
                cccGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTtata                                                                                 >  1:207156/1‑100 (MQ=255)
                  cGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAgg                                                                               <  1:281820/100‑1 (MQ=255)
                  cGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAgg                                                                               <  1:428163/100‑1 (MQ=255)
                      cGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTa                                                                           <  2:1516387/100‑1 (MQ=255)
                        gTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAg                                                                         >  2:2285175/1‑100 (MQ=255)
                        gTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAg                                                                         >  2:818229/1‑100 (MQ=255)
                         ttCTGGTAAAAAATACAATCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGa                                                                        <  2:1526418/100‑1 (MQ=255)
                         ttCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAg                                                                         <  2:1105680/99‑1 (MQ=255)
                         ttCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGa                                                                        <  2:1126764/100‑1 (MQ=255)
                         ttCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGa                                                                        <  1:72591/100‑1 (MQ=255)
                          tCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAAGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGAc                                                                       >  1:209656/1‑100 (MQ=255)
                           cTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACa                                                                      >  1:18509/1‑100 (MQ=255)
                            tGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTTCGCTTTTTTTATAGGTTTAAGACaa                                                                     <  2:1887558/100‑1 (MQ=255)
                            tGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGCTTTAAGACaa                                                                     >  2:923710/1‑100 (MQ=255)
                             tctAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAAt                                                                    <  1:2931572/98‑1 (MQ=255)
                             ggTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAAt                                                                    <  2:1555792/100‑1 (MQ=255)
                             ggTAAAAAATACAAGCAGTGCCATGGCCGACTGCAATAAAAGCTAACTGTTGAAGTAAAATGCGCAGGATTCTCCGCCTTTTTTATAGGTTTAAGACAAt                                                                    >  2:1437641/1‑100 (MQ=255)
                               tAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATg                                                                   <  1:2458471/99‑1 (MQ=255)
                               tAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGACGCAGAATTCTACAACTTTTTTATAAAATTAAGACaa                                                                     >  1:3548904/1‑97 (MQ=255)
                                aaaaaaTACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAAtaga                                                                 >  2:2931546/1‑97 (MQ=255)
                                 aaaaaTACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGaaa                                                                >  2:2316221/1‑100 (MQ=255)
                                 aaaaaTACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTACGCCTTTTTTATAGGTTTAAGACAATGaaa                                                                >  1:2722150/1‑100 (MQ=255)
                                    aaTACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAg                                                             <  2:3485743/100‑1 (MQ=255)
                                       aCAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTg                                                          <  2:3743893/100‑1 (MQ=255)
                                          aGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAGAGCTGCaa                                                       >  1:3359908/1‑100 (MQ=255)
                                           gCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTACGAATTTTTTATAGGTTTAAGACAATGAAAAAGCTGCaaa                                                      >  1:3379196/1‑100 (MQ=255)
                                              gTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTg                                                   >  1:1787167/1‑100 (MQ=255)
                                              gTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTg                                                   >  2:3866382/1‑100 (MQ=255)
                                                gCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCaaa                                                      >  2:3827960/1‑95 (MQ=255)
                                                    tGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtag                                             <  2:769397/100‑1 (MQ=255)
                                                    tGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCGGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtag                                             <  2:3682190/100‑1 (MQ=255)
                                                     ggCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtagg                                            <  2:3900445/100‑1 (MQ=255)
                                                     ggCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtag                                             >  2:316535/1‑99 (MQ=255)
                                                      gccgccTGCAATAAAAGCTAACTGTTGAAGTACAATGCGCAGGATTCTGCGCCTTTTTTATAGGATTAAGACAATGAAAAAGCTGCAAATTGCggtaggt                                           >  1:483/1‑100 (MQ=255)
                                                      gccgccTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtaggt                                           >  1:2207035/1‑100 (MQ=255)
                                                      gacgccTGCAATAGAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtaggt                                           >  1:2642331/3‑100 (MQ=255)
                                                       ccgccTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCggtaggta                                          >  2:1308176/1‑100 (MQ=255)
                                                         gccTGCAATAAAAGCTAACTGTAGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTACGtatt                                        >  2:1789717/1‑100 (MQ=255)
                                                            tGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTattatt                                     >  1:269649/1‑100 (MQ=255)
                                                            tGCAATAAAAGCTAACAGATGAAGTAAAAGGCGCAGGATGCTGTGCCTTTTTTATAGGTTTAAGACAATGAAATAACTGCAAATTGCggttggtagt                                        >  2:2329774/1‑95 (MQ=255)
                                                              cAATAAAAGCTAACTGTTTAAGTAAAAGGTGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTc                                    <  1:2510630/99‑1 (MQ=255)
                                                              cAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCg                                   <  2:3652114/100‑1 (MQ=255)
                                                               aaTAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCg                                   >  1:538528/1‑99 (MQ=255)
                                                               aaTAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGc                                  <  2:17431/100‑1 (MQ=255)
                                                                  aaaaGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAAc                               <  1:2496167/100‑1 (MQ=255)
                                                                      gCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACgaga                           >  1:2191105/1‑100 (MQ=255)
                                                                           cTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTACGGTAGGTATTATTCGCAACGAGAACAAt                      >  1:2520579/1‑100 (MQ=255)
                                                                            tGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATg                     <  2:609285/100‑1 (MQ=255)
                                                                             gTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTTCGGTAGGTACTATTAGCAACGAGAACAATGa                    >  2:102768/1‑100 (MQ=255)
                                                                             gTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGa                    >  1:3604195/1‑100 (MQ=255)
                                                                             gTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGa                    >  2:2836922/1‑100 (MQ=255)
                                                                             acctataaaaaaGGCGCAGAATCCTGCGTCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGa                    <  1:1959283/92‑1 (MQ=38)
                                                                              ttGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGTTTTAAGATAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGaa                   <  1:1003616/100‑1 (MQ=255)
                                                                                tataaaaaaGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAAt                 >  1:1767737/6‑100 (MQ=38)
                                                                                 aaGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATc                >  1:1278134/1‑100 (MQ=255)
                                                                                  aGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGAGGTAGGTATTATTCGCAACGAGAACAATGAAATCt               >  2:849356/1‑100 (MQ=255)
                                                                                  aGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTATAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAATAATGAAATCt               >  2:2081125/1‑100 (MQ=255)
                                                                                   gTAAAAGGCGCAGGATTCTGCGTCTTTTTTATAGGTTGAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCtt              <  2:2647510/100‑1 (MQ=255)
                                                                                   gTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCtt              <  2:508403/100‑1 (MQ=255)
                                                                                   aaaaaaGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCtt              >  2:2629004/3‑100 (MQ=255)
                                                                                    tAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGAAGGTATTATTCGCAACGAGAACAATGAAATCttt             >  1:2984078/1‑100 (MQ=255)
                                                                                    aaaaaGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCttt             >  1:3787761/2‑100 (MQ=255)
                                                                                     aaaaGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCt               >  2:3372979/1‑97 (MQ=255)
                                                                                     aaaaGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTta            <  2:197084/100‑1 (MQ=255)
                                                                                      aaaGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAAAAATGAAATATTtat           >  2:3118705/1‑100 (MQ=255)
                                                                                       aaGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTtata          >  1:990397/1‑100 (MQ=255)
                                                                                       aaGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTtata          >  1:371203/1‑100 (MQ=255)
                                                                                       aaGGCGCAGAATCCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAAGTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCtgtgta          >  2:2887798/1‑95 (MQ=255)
                                                                                        aGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATaa         >  2:1216157/1‑100 (MQ=255)
                                                                                         ggCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGATAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAAc        <  1:323586/100‑1 (MQ=255)
                                                                                          gcgcAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAAcg       >  1:1962552/1‑100 (MQ=255)
                                                                                            gcAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAAcgc      >  1:3930045/1‑99 (MQ=255)
                                                                                             cAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGt    >  2:482914/1‑100 (MQ=255)
                                                                                             cAGGATTCTGCGACTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGt    >  1:2580176/1‑100 (MQ=255)
                                                                                              aGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAAcgcg     <  1:846457/98‑1 (MQ=255)
                                                                                               ggATTCTGCGCCTTTTTTTTTTTTTTTTGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGGAACAAGAACAATGACATCTTTATAAGACGTcg  >  2:1710901/1‑100 (MQ=255)
                                                                                               ggATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAAAACAATGAAATCTTTATAACGCGTcg  >  1:817168/1‑100 (MQ=255)
                                                                                               ggATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAAAGAGAACAATGAAATCTTTATAACGCGTcg  >  1:1605848/1‑100 (MQ=255)
                                                                                                |                                                                                                  
GACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAGCAGTGCCATGGCCGCCTGCAATAAAAGCTAACTGTTGAAGTAAAAGGCGCAGGATTCTGCGCCTTTTTTATAGGTTTAAGACAATGAAAAAGCTGCAAATTGCGGTAGGTATTATTCGCAACGAGAACAATGAAATCTTTATAACGCGTCG  >  NC_000913/110917‑111111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: