Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 732,503 G→A 17.2% R974Q (CGG→CAG)  rhsC → Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913732,5030GA17.2% 197.2 / 34.0 99R974Q (CGG→CAG) rhsCRhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base G (43/39);  new base A (11/6);  total (54/45)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-03

CACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGG  >  NC_000913/732406‑732600
                                                                                                 |                                                                                                 
cACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCAACCACGGGTACCATTACGACAGTCAGCACAGGc                                                                                                 >  1:2826962/1‑100 (MQ=255)
cACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAACGCACCCACCAGTACCATTACGACAGTCAGCACCGGc                                                                                                 >  1:2071287/1‑100 (MQ=16)
 aCGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCt                                                                                                <  1:1365461/100‑1 (MQ=33)
  cgtcAGTCTGACATAGAAATCCTACCTCATCCCTTAATATGTTATCCGCACGTATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCt                                                                                                <  1:3330740/96‑1 (MQ=11)
  cGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTg                                                                                               <  2:2784167/100‑1 (MQ=32)
  cGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTg                                                                                               >  2:1782385/1‑100 (MQ=32)
   ggCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTgg                                                                                              <  1:2207051/100‑1 (MQ=32)
    gCAGGCTGACAGAGAAACCCGCCCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCGCCCACCGGTACCATTACGACAGTCAGCACCAGCCGGt                                                                                             <  2:1351606/100‑1 (MQ=11)
      aGGCTGACAGAGAAAACCGACCTCATCCCGGGAGGGGTTATCCGAACGGATGATGAGCGCACCCACCGGTACCATTACTACTGTCAGCACCGGCTGGTGc                                                                                           <  2:1239351/100‑1 (MQ=11)
      aGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGc                                                                                           <  1:2363776/100‑1 (MQ=33)
       ggCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCa                                                                                          >  1:851790/1‑100 (MQ=33)
        gCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATTATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCAc                                                                                         <  2:3321556/100‑1 (MQ=18)
        gCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTTGTGc                                                                                           <  2:871903/98‑1 (MQ=17)
        gCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCAc                                                                                         >  2:1599915/1‑100 (MQ=32)
        gCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCAc                                                                                         <  1:3389733/100‑1 (MQ=33)
         cTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACt                                                                                        >  2:1770877/1‑100 (MQ=33)
         cTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACt                                                                                        <  1:85476/100‑1 (MQ=33)
         cTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACt                                                                                        >  1:598766/1‑100 (MQ=33)
         cTGACAGAGAAAACCGACCTCATCCCCGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACt                                                                                        <  2:3784481/100‑1 (MQ=17)
          tGACAGAGAAAACTGACCTCATCCCGGAAGGGGTTATCCGCACGGATGAGGAGCGCACCCACCGGTACGTTTACGACAGTCAGCACCGGCTGGTGCACTa                                                                                       <  2:3423605/100‑1 (MQ=11)
          tGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTa                                                                                       >  1:3091283/1‑100 (MQ=32)
          tGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCAGCCGGTGctgac                                                                                       <  2:1769247/100‑5 (MQ=11)
          tGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCAGCCGGTGctgac                                                                                       <  1:891012/100‑5 (MQ=11)
           gACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTac                                                                                      >  1:3928669/1‑100 (MQ=32)
           gACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCAGCCGGTGctgact                                                                                      <  2:3609670/100‑6 (MQ=11)
            aCAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCTCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCAGCCGGTGctgactg                                                                                     <  1:3157424/100‑7 (MQ=11)
            aCAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCTCCGGTACCATTACGACAGTCAGCACCAGCCGGTGctgactg                                                                                     <  2:1249446/100‑7 (MQ=11)
            aCAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTaca                                                                                     <  1:2944829/100‑1 (MQ=32)
             cAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTacac                                                                                    <  1:1222657/100‑1 (MQ=31)
             cAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTacac                                                                                    <  2:3662955/100‑1 (MQ=31)
             cAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTacac                                                                                    <  1:1665732/100‑1 (MQ=31)
             cAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTacac                                                                                    <  2:2251303/100‑1 (MQ=31)
              agagAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATAATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACAcg                                                                                   >  1:85686/1‑100 (MQ=11)
                                               ccGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGt                                                  >  2:219144/1‑100 (MQ=11)
                                                cGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGt                                                  <  1:3742046/99‑1 (MQ=11)
                                                cGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTc                                                 >  1:2080201/1‑100 (MQ=31)
                                                cGCACGGATGATAAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACAAAATATGCAGAGCCGCTGGTCGAAAGTc                                                 >  2:1266373/1‑100 (MQ=9)
                                                  gaCGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCATAGCCGCTGGTCGAAAGTCg                                                <  2:1983791/98‑1 (MQ=17)
                                                   aCGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGc                                               <  2:2428527/99‑1 (MQ=32)
                                                       atgatgAGCGCACCCACCGGTACCATTACTACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCt                                          <  1:3425670/100‑1 (MQ=18)
                                                       atgatgAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCt                                          >  2:3220094/1‑100 (MQ=32)
                                                        tgatgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCtt                                         <  2:1170066/100‑1 (MQ=33)
                                                         gatgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCttt                                        >  1:1498890/1‑100 (MQ=32)
                                                         gatgaGCGCACACACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCtct                                        >  2:43158/1‑98 (MQ=18)
                                                          atgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGCAACACTATGCAGAGCCGCTGGTCGAAGGTCGCTATGttt                                        >  2:2453726/1‑99 (MQ=11)
                                                          atgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGTTCGAAAGTCGCTATCtt                                         <  2:1408807/98‑1 (MQ=17)
                                                          atgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTa                                       <  1:3193648/100‑1 (MQ=33)
                                                          atgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTa                                       <  1:2051309/100‑1 (MQ=33)
                                                           tgaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCCCGGCGAAAGTCGCTATCTTTAc                                      >  2:3425533/1‑100 (MQ=255)
                                                            gaGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACg                                     <  2:393253/100‑1 (MQ=33)
                                                             aGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGa                                    >  1:343514/1‑100 (MQ=32)
                                                             aGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGa                                    >  1:61408/1‑100 (MQ=33)
                                                             aGCGCACCCACCGGTACCATTACGACAGTCAGCACAGGCTGGTGCACTACACGCGGCCACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGa                                    >  2:1385736/1‑100 (MQ=12)
                                                             aGCGCACCCACCGGTAACATTACGACAGTCAGCACCGGCTGGTGCACTACACCCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGa                                    >  2:14617/1‑100 (MQ=12)
                                                              gcgcACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATACAAAACCACTGGTCGAAAGTCGCTATCTTTACGAc                                   >  1:1962829/1‑100 (MQ=11)
                                                               cgcACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGCCACAATATGCAGAGCCGCTGGCCGAAAGTCGCTATCTTTACGAcc                                  >  2:3468219/1‑100 (MQ=12)
                                                                 cacccaccGGTACCATTACGACAGTCAGCACCGCCTGGTGCACTACACCCGGACACAATATGCACAGCTCCTACGCGAAAGTCGCTATCTTTACGAcc                                  >  2:1457147/1‑98 (MQ=11)
                                                                  acccaccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGc                               >  2:3857307/1‑100 (MQ=32)
                                                                   cccaccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCg                                >  2:2910973/1‑98 (MQ=32)
                                                                   cccaccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCt                              <  2:1197891/100‑1 (MQ=33)
                                                                     ccccGGTACCATTACGACAGTCAGCACAGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTg                             >  2:2788864/3‑99 (MQ=17)
                                                                      accGGTACCATTACGACAGTCAGCACCGGCTGGTGCATTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTggg                           <  1:2067729/100‑1 (MQ=17)
                                                                       ccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGc                          <  1:2864314/100‑1 (MQ=33)
                                                                       ccGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGc                          <  1:86270/100‑1 (MQ=32)
                                                                           tACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACAAAATATGCAGAGCCGTTGGTCGAAAGTCGCTATCTTTATGACTCGCTGGGCCGCa                      <  2:1601488/100‑1 (MQ=11)
                                                                             ccATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAgg                    >  2:2229181/1‑100 (MQ=33)
                                                                              cATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAggg                   <  1:2053596/100‑1 (MQ=32)
                                                                              cATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAggg                   >  2:3008912/1‑100 (MQ=32)
                                                                                ttACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTg                 <  2:409531/100‑1 (MQ=33)
                                                                                 tACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTg                 >  2:1738316/1‑99 (MQ=32)
                                                                                  aCGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGc               >  2:3616223/1‑100 (MQ=32)
                                                                                   cGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGc               >  2:2856794/1‑99 (MQ=33)
                                                                                   cGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCa              >  1:3850347/1‑100 (MQ=33)
                                                                                   cGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCa              <  1:3439274/100‑1 (MQ=33)
                                                                                   cGACAGTCAGCACCGGCTGGTGAACTACACGCGGACACTATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGc               <  2:3666535/99‑1 (MQ=12)
                                                                                   cGACACTCAGCACCGGCTGGTGCACTACAGTGGGACATAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGc               <  2:3915079/99‑1 (MQ=11)
                                                                                    cgtgtagtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCaa             >  1:1789544/9‑100 (MQ=11)
                                                                                     gtgtagtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACACGCTGGGCCGCAGGGTGGCaaa            >  1:830515/8‑100 (MQ=11)
                                                                                     aCAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTAGCTATCTTTACGACAAGCTAGGCCACAGGGTGGCaaa            >  1:1894112/1‑100 (MQ=11)
                                                                                      tgtagtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCaaaa           >  2:1012741/7‑100 (MQ=11)
                                                                                      cAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCaaaa           >  1:2924075/1‑100 (MQ=33)
                                                                                       gtagtgCACCAGCCGGTGCACTACACGCGGAAACAATATGCAGAGACGCTGGTCGAAAGTCGCGATCTTAACGACACGCTGGGCCGCAGGGGGGCAAAAc          >  2:786942/6‑100 (MQ=11)
                                                                                       gtagtgCACCAGCCGGTGCACTAAAAGAGGACACAATATGCAGAGACGATGGTAGAAAGTCGCTATCTTTACGAAACGCTGGGCCGGAGGGTGGCaa             >  2:728097/6‑97 (MQ=2)
                                                                                       aGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAAc          <  1:292898/100‑1 (MQ=32)
                                                                                        tagtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACg         >  1:1963115/5‑100 (MQ=11)
                                                                                        tagtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAAGGTGGCAAAAc          >  1:2681838/5‑99 (MQ=11)
                                                                                        gTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACg         >  2:2905514/1‑100 (MQ=32)
                                                                                         agtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACgg        >  2:1020391/4‑100 (MQ=255)
                                                                                         agtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACgg        >  2:509615/4‑100 (MQ=255)
                                                                                         agtgCACCAGCCGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACgg        >  1:2335715/4‑100 (MQ=255)
                                                                                         agtgCACCAGCCGGTGCACTACACGCAGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGAACCGCTGGGCCGCAGGGTGGCAAAACg         >  1:2375987/4‑99 (MQ=11)
                                                                                          cAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACggg       >  2:3573219/1‑100 (MQ=32)
                                                                                           aGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGt      <  2:2071268/100‑1 (MQ=33)
                                                                                            gCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACggg       >  2:3923891/1‑98 (MQ=32)
                                                                                            gCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTa     >  2:2087615/1‑100 (MQ=35)
                                                                                             cACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTAt    >  2:3138257/1‑100 (MQ=35)
                                                                                              aCCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATg   <  1:2802687/100‑1 (MQ=35)
                                                                                               ccGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATgg  >  1:1092849/1‑100 (MQ=35)
                                                                                               ccGGCTGGTGCACTACACACGTACACAATATACAGAGCCGCTAGTCGAAAGTCGCTATCTTTAGGCCCAGCCGGGCCGCAGGGTGGCCAAAGGGGTATg   >  2:1825461/1‑99 (MQ=11)
                                                                                                 |                                                                                                 
CACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGCACCCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGCAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTATGG  >  NC_000913/732406‑732600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: