BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	805,312	G→T	15.8%	E604* (GAG→TAG) ‡	ybhJ →	aconitase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	805,312	0	G	T	15.8%	367.3 / 54.7	148	E604* (GAG→TAG)	ybhJ	aconitase family protein
Reads supporting (aligned to +/- strand): ref base G (64/59); new base T (7/16); total (71/77)
Fisher's exact test for biased strand distribution p-value = 7.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-02

Only 100 of 151 total aligned reads displayed.

CTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAA  >  NC_000913/805213‑805409

cTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCGGGCGGGGAATGTCAGCg                                                                                                   >  2:3931352/1‑100 (MQ=255)

 tGGCGGAGTTTACCCTGTCTCGCCGCGATCCAGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGa                                                                                                  >  1:2849204/1‑100 (MQ=255)

  ggCGGAGTTTACCCTGTCTCGCCGCGATTCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAg                                                                                                 <  1:3518421/100‑1 (MQ=255)

  ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAg                                                                                                 >  1:192669/1‑100 (MQ=255)

  ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAg                                                                                                 <  2:2711940/100‑1 (MQ=255)

   gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGc                                                                                                >  2:1356098/1‑100 (MQ=255)

   gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGc                                                                                                <  2:2169346/100‑1 (MQ=255)

       aGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGAc                                                                                            <  2:463904/100‑1 (MQ=255)

        gTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACa                                                                                           >  1:1632172/1‑100 (MQ=255)

         tttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACat                                                                                          <  1:2136210/100‑2 (MQ=255)

          ttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTTCTAAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACaga                                                                                         >  1:1199711/1‑100 (MQ=255)

          ttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACatt                                                                                         <  2:2193611/100‑3 (MQ=255)

          ttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACatt                                                                                         <  2:600047/100‑3 (MQ=255)

           tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGAAaga                                                                                         >  1:1957040/1‑99 (MQ=255)

            ttccgatctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcc                                                                                       <  2:3054295/94‑5 (MQ=255)

            aCCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcc                                                                                       <  1:2250302/100‑5 (MQ=255)

             nccggTCTCGACTCGATCCCTGTCATGTTACCAGAAGCAAATCGACTGCTGAGCCCGACAATCAGCGTCTGGCGGGGAATGTCAGCGCGCTGACagagc                                                                                       >  2:3233209/5‑98 (MQ=255)

             cccTGTCTCGCCGCGATCCCGGTTATGTTATCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattccc                                                                                      <  2:3708906/100‑6 (MQ=255)

             cccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattccc                                                                                      <  1:933492/100‑6 (MQ=255)

             cccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattccc                                                                                      <  1:3030672/100‑6 (MQ=255)

             cccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCACGCTGACattccc                                                                                      <  1:2629926/100‑6 (MQ=255)

             cccTGTCTCGCCGCGATCCCGCTTATTTTAGCAGATGTAAATCTACTGCTCATCTGTAAAATCAGCGTCTATCGCTCAATGTTATCGAGCTGACaga                                                                                         <  2:1867742/97‑1 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGTAGAAGTAAAGCTTCTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcccc                                                                                     <  1:3275026/100‑7 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcccc                                                                                     <  1:3803425/100‑7 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcc                                                                                       <  2:2071984/98‑5 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgtg                                                                                     <  2:1681635/100‑1 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgtg                                                                                     >  1:443944/1‑100 (MQ=255)

               cTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcccc                                                                                     <  1:55286/99‑7 (MQ=255)

               cTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgtgt                                                                                    >  2:3659391/1‑100 (MQ=255)

                    tcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcg                                                                               <  1:1716565/100‑1 (MQ=255)

                    tcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcg                                                                               <  1:782719/100‑1 (MQ=255)

                     cgccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcgc                                                                              >  1:993903/1‑100 (MQ=255)

                      gccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTTACAGAGGTGTTTgcgcg                                                                             <  2:2559694/100‑1 (MQ=255)

                      gccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcgcg                                                                             >  1:3371067/1‑100 (MQ=255)

                             ttccgatcttGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAg                                                                      <  1:451669/91‑1 (MQ=255)

                              cccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGc                                                                     >  1:837671/1‑100 (MQ=255)

                              cccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGc                                                                     <  2:564818/100‑1 (MQ=255)

                               ccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCa                                                                    <  2:1382318/100‑1 (MQ=255)

                                     aTGTTAGCAGAAGTAAAGCGACTGCTGATCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGc                                                              <  1:1726108/100‑1 (MQ=255)

                                     aTGTTAGCAGAAGTAAAGCGACTGCTGAGTTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTgn                                                              <  2:2347399/100‑2 (MQ=255)

                                      tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCt                                                             >  1:1252576/1‑100 (MQ=255)

                                      tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCt                                                             >  2:1966170/1‑100 (MQ=255)

                                      tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCt                                                             >  1:2986714/1‑100 (MQ=255)

                                           gCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCa                                                        >  2:2625327/1‑100 (MQ=255)

                                              gAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGAAAGAGGTGTTTGCGAGCATTAAGCAGATTGCTGGTCAGGa                                                     >  1:3571040/1‑100 (MQ=255)

                                                  tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCat                                                 <  1:2500150/100‑1 (MQ=255)

                                                   aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCata                                                >  1:2181714/1‑100 (MQ=255)

                                                     aGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                              <  1:3785099/100‑1 (MQ=255)

                                                     aGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                              <  1:1674177/100‑1 (MQ=255)

                                                         aCTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATc                                          <  2:3879067/100‑1 (MQ=255)

                                                           tgctgAGCTTGAATATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCat                                                 <  1:2681054/91‑1 (MQ=255)

                                                             ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCt                                      <  1:3430128/100‑1 (MQ=255)

                                                              tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTg                                     >  1:1216721/1‑100 (MQ=255)

                                                               gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAAGAGCATATTGATCCGCTGc                                    >  1:1259433/1‑100 (MQ=255)

                                                                 gCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTAGTCAGGAGCATATTGATCCGCTGCaa                                  >  1:863430/1‑100 (MQ=255)

                                                                  cTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaaa                                 >  2:668165/1‑100 (MQ=255)

                                                                   tGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAAc                                <  2:1700682/100‑1 (MQ=255)

                                                                    ggAACATCAGCGTCTGGCGGAGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACt                               >  2:462640/1‑100 (MQ=255)

                                                                    ggAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACt                               >  1:1790336/1‑100 (MQ=255)

                                                                       aaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                            >  2:3442321/1‑100 (MQ=255)

                                                                          tCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAAtt                         >  2:1388542/1‑100 (MQ=255)

                                                                           cAGCGTCTGGCGGGGAATGTCAGCGTGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                        <  1:1542428/100‑1 (MQ=255)

                                                                           aaGCGTCTGGCGGGGAATGTCAGCGAGATGACAGAGGTGTTTGCGCTCATTAAGCAGATTGCTGGGCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                        >  2:1295736/2‑100 (MQ=255)

                                                                             tcttCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                      <  2:2352912/97‑1 (MQ=255)

                                                                             gCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgc                      >  2:962909/1‑100 (MQ=255)

                                                                              cGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAAGAGCATATTGATCCGCTGCAAACTGAAATTGgca                     >  1:1694305/1‑100 (MQ=255)

                                                                               gTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGGTGGACAAGAGCATATTGATCCGCTGCAAACTGAAATTGgcag                    >  1:1063368/1‑100 (MQ=255)

                                                                               gTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcag                    <  2:3291508/100‑1 (MQ=255)

                                                                               gTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcag                    <  1:3326836/100‑1 (MQ=255)

                                                                                tCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcagc                   >  2:2230328/1‑100 (MQ=255)

                                                                                tCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcaga                   >  1:2352934/1‑99 (MQ=255)

                                                                                 cTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcagca                  <  1:3685678/100‑1 (MQ=255)

                                                                                 cTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcagca                  <  2:2809000/100‑1 (MQ=255)

                                                                                  tGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCAt                 >  2:442416/1‑100 (MQ=255)

                                                                                  tGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCAt                 <  1:896851/100‑1 (MQ=255)

                                                                                   ggCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGTATTTAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                <  1:357561/100‑1 (MQ=255)

                                                                                   ggCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                >  1:273396/1‑100 (MQ=255)

                                                                                   ggCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                >  1:407178/1‑100 (MQ=255)

                                                                                   ggCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                >  2:1129952/1‑100 (MQ=255)

                                                                                    gCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATgg               <  2:3597463/100‑1 (MQ=255)

                                                                                     cGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGt              <  2:443938/100‑1 (MQ=255)

                                                                                      ggggAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTc             <  2:2968243/100‑1 (MQ=255)

                                                                                      ggggAATGTCAGCGAGCTGACAGAGGAGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGCGCATATTGATCCGCTGCAAACTGAAATTGGCAGAATGGTc             >  2:3692485/1‑100 (MQ=255)

                                                                                      cacctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTc             >  1:3504710/7‑100 (MQ=255)

                                                                                        ggAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa           <  2:2986687/100‑1 (MQ=255)

                                                                                        cctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa           >  1:1407614/5‑100 (MQ=255)

                                                                                        cctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa           >  1:2794738/5‑100 (MQ=255)

                                                                                        cctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa           >  2:2920091/5‑100 (MQ=255)

                                                                                           aTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTTATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGc        <  2:1420905/100‑1 (MQ=255)

                                                                                           aTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATg         >  2:1134367/1‑99 (MQ=255)

                                                                                           aTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGc        <  2:3264279/100‑1 (MQ=255)

                                                                                           aTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGc        <  1:3684774/100‑1 (MQ=255)

                                                                                           aTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGc        >  2:1619806/1‑100 (MQ=255)

                                                                                             gTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCgg      <  2:1868592/100‑1 (MQ=255)

                                                                                             gTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTACAAACAAAAATTGGCAGCATGGTCTATGCgg      >  2:104394/1‑100 (MQ=255)

                                                                                               cAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg    >  2:632581/1‑100 (MQ=255)

                                                                                               cAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg    <  1:1379183/100‑1 (MQ=255)

                                                                                               cAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTAGCAGCATGGTCTATGCGGTg    >  2:2241713/1‑100 (MQ=255)

                                                                                                aGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGa   >  1:1958682/1‑100 (MQ=255)

                                                                                                 gCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGTTGGTTATGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaa  <  1:1219889/100‑1 (MQ=255)

CTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAA  >  NC_000913/805213‑805409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`