Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,143,641 G→A 100% H243Y (CAT→TAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,6410GA99.4% 415.2 / ‑6.1 124H243Y (CAT→TAT) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (64/59);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 124 total aligned reads displayed.

ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143544‑1143736
                                                                                                 |                                                                                               
aTCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACt                                                                                               <  1:3098154/100‑1 (MQ=255)
 tCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTg                                                                                              <  1:2747060/100‑1 (MQ=255)
   tgtgACTCGATCTGGTAGTGGCTGATCTGCTGTGTTCCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCTGGTGTCCTAATGCAGCTATATACTGAc                                                                                            <  2:3518685/100‑1 (MQ=255)
   tgtgACTCGAGCTGGGAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACGTAATGCAGCGATATACTGa                                                                                             <  2:2322175/99‑1 (MQ=255)
      gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         >  2:1325277/1‑100 (MQ=255)
       aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGc                                                                                        >  2:2569516/1‑100 (MQ=255)
        ntCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       >  2:1614395/2‑100 (MQ=255)
        cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  1:94694/100‑1 (MQ=255)
        cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  1:3393118/100‑1 (MQ=255)
        cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       >  1:2454062/1‑100 (MQ=255)
          cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     >  2:3393080/1‑100 (MQ=255)
           gATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     <  1:1608190/99‑1 (MQ=255)
           gATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGt                                                                                    >  1:855220/1‑100 (MQ=255)
            aTCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTAGAGTTTGATTTTGTTGCTGAAATCCGGGCGACCTAATGTAGCGATATACTGACGTGCCAGtt                                                                                   >  2:3128462/1‑100 (MQ=255)
            aTCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGtt                                                                                   >  1:1628979/1‑100 (MQ=255)
            aTCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGtt                                                                                   >  1:1629298/1‑100 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATATTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  2:2494386/100‑1 (MQ=255)
                 gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAATTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc                                                                              <  1:776057/100‑1 (MQ=255)
                  tAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCa                                                                             <  1:3071217/100‑1 (MQ=255)
                  tAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCa                                                                             >  1:3307517/1‑100 (MQ=255)
                      ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  2:1634503/1‑100 (MQ=255)
                      ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  2:1799578/1‑100 (MQ=255)
                        cTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCg                                                                       >  1:2517203/1‑100 (MQ=255)
                        cTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCg                                                                       >  2:1050206/1‑100 (MQ=255)
                          gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     >  1:3540075/1‑100 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  >  1:3295509/1‑100 (MQ=255)
                              aGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  <  2:2698886/99‑1 (MQ=255)
                              aGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTAt                                                                 <  1:2790338/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTGCAGTTTGGTTTTGCTGCTGAGATCCGGGTGACCTAATGCAGGGATATGCTGACGTGCCAGTTCGTGCGCTTTCGGGTTagc                                                                <  2:1559560/100‑3 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:442098/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGCTTGATTTTGCCGCTGAAATCCGCGCGACCAAATGCAGCGATAAACAGCCGTGCCAGTCCGAACAATTTCAGCTTaac                                                                >  2:2107397/1‑98 (MQ=255)
                                cGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCg                                                               >  1:1878143/1‑100 (MQ=255)
                                 gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                              <  2:2454039/100‑1 (MQ=255)
                                 gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                              >  2:482817/1‑100 (MQ=255)
                                 gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGa                                                              <  1:153734/100‑1 (MQ=255)
                                   gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAAGCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa                                                            >  2:2089826/1‑100 (MQ=255)
                                    aTCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaa                                                           <  1:2569540/100‑1 (MQ=255)
                                    aTCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaa                                                           <  1:651299/100‑1 (MQ=255)
                                        cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGAAGTGCCAGTTCGAGCACTTTCGGGTTATCTATAAGGAt                                                       >  2:2406231/1‑100 (MQ=255)
                                         gCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAtt                                                      >  2:3087152/1‑100 (MQ=255)
                                            ggTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   >  2:322367/1‑100 (MQ=255)
                                            ggTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   <  2:2333275/100‑1 (MQ=255)
                                            ggTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   <  2:2851556/100‑1 (MQ=255)
                                               gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg                                                <  1:1089058/100‑1 (MQ=255)
                                                tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               <  1:2549235/100‑1 (MQ=255)
                                                tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               >  2:1015164/1‑100 (MQ=255)
                                                 aCAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGAt                                              <  1:3429665/100‑1 (MQ=255)
                                                  cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             <  2:266266/100‑1 (MQ=255)
                                                  cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             <  1:608113/100‑1 (MQ=255)
                                                  cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAACACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             >  1:262105/1‑100 (MQ=255)
                                                   aGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGt                                            <  1:3443883/100‑1 (MQ=255)
                                                   aGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAACACTATCGGGTTATCGATAAGGATTTCGCCGATGt                                            >  1:1074589/1‑100 (MQ=255)
                                                    gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                           >  2:2815764/1‑100 (MQ=255)
                                                     tttGATTTTGCTGCTGAAATCCGGGCGATCTTATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  1:106381/100‑1 (MQ=255)
                                                     tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  1:3803076/100‑1 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGTGATATACTGACGTGGCAGGTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:1126579/100‑1 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:2433078/100‑1 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:2311985/100‑1 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         >  2:830599/1‑100 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGAGCGACCTAATGCAGCGATATACTGACGTGCTAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCAGATGTCCt                                         >  2:239294/1‑100 (MQ=255)
                                                        gATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa                                       <  2:855210/100‑1 (MQ=255)
                                                          ttttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                      <  1:1727845/99‑1 (MQ=255)
                                                          ttttCCTGCTGAAATCCTGGCGACCTAATGAAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGTTTATCGATAAGGATTTCGCCGATGTCCTGa                                       <  2:1891499/98‑1 (MQ=255)
                                                            ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgta                                   <  1:3248791/100‑1 (MQ=255)
                                                              gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 <  1:1989726/100‑1 (MQ=255)
                                                                tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  2:2144902/1‑100 (MQ=255)
                                                                tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  1:550685/1‑100 (MQ=255)
                                                                 gctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagtaa                              >  2:1781044/1‑100 (MQ=255)
                                                                  ctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagtaa                              >  2:3478137/1‑99 (MQ=255)
                                                                  ctgAAATCCGGGCGACCTAATGCAGAGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt                             >  2:2474652/1‑100 (MQ=255)
                                                                   tgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATc                            >  1:1481317/1‑100 (MQ=255)
                                                                    gAAATCCGGGCGACCTACTGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcg                           <  2:2334537/100‑1 (MQ=255)
                                                                     aaaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgc                          <  2:787347/100‑1 (MQ=255)
                                                                      aaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgcg                         >  2:2311688/1‑100 (MQ=255)
                                                                      aaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgcg                         >  2:1880916/1‑100 (MQ=255)
                                                                       atacGGGCGACATAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        <  2:2523633/97‑1 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCCATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGCTAAGGATTTCGTCGATGTCATGACgtaacta                               >  2:2431465/1‑93 (MQ=255)
                                                                         ccGGGCGACCTAATGCAGCGATATACTGACGTGGCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      <  2:53916/100‑1 (MQ=255)
                                                                         ccGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      >  2:1534840/1‑100 (MQ=255)
                                                                          cGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt                     >  2:512981/1‑100 (MQ=255)
                                                                          cGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGACGATGTACTGACGTAAGTAATCGCGGAAt                     >  1:1449956/1‑100 (MQ=255)
                                                                           gggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGCTAAGGATTTCGCCGATGTCCTGACGTAAGGAATCGCGGAATg                    >  2:532942/1‑100 (MQ=255)
                                                                           gggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg                    <  2:3297394/100‑1 (MQ=255)
                                                                           gggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg                    <  1:986323/100‑1 (MQ=255)
                                                                             gCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  >  2:392441/1‑100 (MQ=255)
                                                                             gCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  <  1:1230230/100‑1 (MQ=255)
                                                                             gCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  <  1:2837898/100‑1 (MQ=255)
                                                                              cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGATATCGATAAGGATTTCGACGATGTCCTGACGTAAGTAATCGCGGAATgcg                  >  2:3364504/1‑99 (MQ=255)
                                                                                aCCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGa               >  2:555018/1‑100 (MQ=255)
                                                                                 ccTAATGCAGCGATATACCGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa              >  2:778213/1‑100 (MQ=255)
                                                                                   tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg            <  2:3488883/100‑1 (MQ=255)
                                                                                    aaTGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGa           >  2:1616377/1‑100 (MQ=255)
                                                                                      tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  1:2252134/1‑100 (MQ=255)
                                                                                       gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        <  2:2675298/100‑1 (MQ=255)
                                                                                       gCAGCGATATACTGACGTGCCAGTTCGAGCAATTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        <  1:203321/100‑1 (MQ=255)
                                                                                        cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       >  1:1858200/1‑100 (MQ=255)
                                                                                        cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       >  1:3315137/1‑100 (MQ=255)
                                                                                         aGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACg      <  2:1968646/100‑1 (MQ=255)
                                                                                          gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGAATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt     >  1:40391/1‑100 (MQ=255)
                                                                                             atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  1:1534855/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143544‑1143736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: