BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,226,832	T→G	19.0%	F78C (TTT→TGT)	ycgJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,832	0	T	G	19.0%	300.7 / 74.8	137	F78C (TTT→TGT)	ycgJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (57/54); new base G (15/11); total (72/65)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-02

Only 100 of 143 total aligned reads displayed.

TGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCA  >  NC_000913/1226733‑1226931

tgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAAtt                                                                                                     <  1:2540291/100‑1 (MQ=255)

   aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTAc                                                                                                  >  1:3077553/1‑100 (MQ=255)

    cTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACa                                                                                                 >  1:2333763/1‑100 (MQ=255)

      cAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                                                               >  1:2879016/1‑100 (MQ=255)

      cAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                                                               <  1:432777/100‑1 (MQ=255)

       aGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTc                                                                                              <  2:549972/100‑1 (MQ=255)

        gATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGTGATAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCg                                                                                             <  1:1160325/100‑1 (MQ=255)

         aTGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGAGAATTTACATTAGc                                                                                            >  2:3317313/1‑100 (MQ=255)

          tGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGAT‑CCAGCGAATGTAAATTCGCtg                                                                                          <  2:3311969/100‑2 (MQ=255)

          tGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAAGCTACGATCCCAGCGAATTTACATTCGCt                                                                                           >  1:1643114/1‑100 (MQ=255)

           ggCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtg                                                                                          <  1:3644618/100‑2 (MQ=255)

            gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAGAACTTACAATCATTACAAGGCTACGATCCCTGCGAATGTAAATTCGCtgg                                                                                         <  2:1506671/100‑3 (MQ=255)

            gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTaa                                                                                         <  1:3676044/100‑1 (MQ=255)

            gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTaa                                                                                         >  2:3555083/1‑100 (MQ=255)

            gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                         <  1:61527/100‑3 (MQ=255)

            gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                         <  1:1919252/100‑3 (MQ=255)

            gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtgg                                                                                         <  1:1397870/100‑3 (MQ=255)

              aTTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACg                                                                                       <  1:3237173/100‑1 (MQ=255)

                 tccgaagagTTAACAAGGAAATATCTCGACGAAAAATCCGCTGAAAACTTACAATCATTACTAGGTTACGATCCCTGTGAATGTAAATTCGCtggga                                                                                       <  2:766683/92‑5 (MQ=255)

                  ccccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGgt                                                                                     >  2:3478319/1‑98 (MQ=255)

                   cccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                                                                  >  2:3818231/1‑100 (MQ=255)

                    ccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGACTACGATCCCAGCGAATTTACATTCGCTAACGGTGTAt                                                                                 >  1:1899432/1‑100 (MQ=255)

                      agagggaaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTAttt                                                                               <  2:3009959/94‑1 (MQ=255)

                         gTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGc                                                                            <  1:3566687/100‑1 (MQ=255)

                           tAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGa                                                                          <  2:177252/100‑1 (MQ=255)

                           tAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGa                                                                          >  2:18128/1‑100 (MQ=255)

                            aaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGAt                                                                         >  2:2629219/1‑100 (MQ=255)

                              cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTATGATACCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGt                                                                       >  1:3273582/1‑100 (MQ=255)

                              cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGt                                                                       >  1:3154325/1‑100 (MQ=255)

                              cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGt                                                                       >  1:2872272/1‑100 (MQ=255)

                               aaGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                                                      <  2:3077525/100‑1 (MQ=255)

                                aGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTa                                                                     <  1:3653204/100‑1 (MQ=255)

                                 ggAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                    >  2:909472/1‑100 (MQ=255)

                                 ggAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                    >  1:1011285/1‑100 (MQ=255)

                                 ggAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGAGTATTTTGCGATGTTaa                                                                    >  1:1310347/1‑100 (MQ=255)

                                   aaaTATCATGGCGAAAAAGCCGGTGAAAACTTACAATCATTACAAGTCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAg                                                                  <  2:1117956/100‑1 (MQ=255)

                                    aaTATCTTGGTGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAg                                                                  <  1:1427661/99‑1 (MQ=255)

                                       atCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAATCGCTAACGGTGTATTTTGCGATGTTAAAGaaaa                                                              <  1:1825921/100‑1 (MQ=255)

                                         ctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAt                                                            >  1:1485416/1‑100 (MQ=255)

                                          tcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAtt                                                           >  1:1086552/1‑100 (MQ=255)

                                           cGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTa                                                          <  1:2521583/100‑1 (MQ=255)

                                            ggCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTAt                                                         <  2:1853363/100‑1 (MQ=255)

                                             gCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATg                                                        <  1:1133963/100‑1 (MQ=255)

                                             gCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATACCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATg                                                        <  2:3214042/100‑1 (MQ=255)

                                               gAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTGAAAGAAAAATTATGTc                                                      >  2:2286937/1‑100 (MQ=255)

                                                 aaaaGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGt                                                    <  1:690219/100‑1 (MQ=255)

                                                  aaaGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGt                                                    >  1:1844648/1‑99 (MQ=255)

                                                   aaGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtga                                                  <  1:279967/100‑1 (MQ=255)

                                                       cGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                              <  2:1186568/100‑1 (MQ=255)

                                                         cTGAAAACTTACAATCATTACAAGGCTACGATCCAAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                              >  2:914079/1‑98 (MQ=255)

                                                               aCTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAttt                                      <  1:1041920/100‑1 (MQ=255)

                                                                cTTACAATCATTACAAGGCTACGATCTCAGTGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAtttt                                     <  1:3217027/100‑1 (MQ=255)

                                                                cTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAtttt                                     >  1:567092/1‑100 (MQ=255)

                                                                  tACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                   <  1:853869/100‑1 (MQ=255)

                                                                  tACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                   >  2:3467036/1‑100 (MQ=255)

                                                                  tACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                   <  2:3664961/100‑1 (MQ=255)

                                                                  tACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                   <  1:1667281/100‑1 (MQ=255)

                                                                  tACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTg                                    >  2:332149/1‑99 (MQ=255)

                                                                   aCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgt                                  <  2:1785596/100‑1 (MQ=255)

                                                                       tCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTgg                              >  2:3847005/1‑100 (MQ=255)

                                                                         aTTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGAt                            <  1:449164/100‑1 (MQ=255)

                                                                         aTTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGAt                            >  2:2834221/1‑100 (MQ=255)

                                                                          ttACAAGGCTTCGATCCCAGCGAATTTACTTTCGCTAACGGTGTATTTTGCGATTTTAAAGTAAAAGTATGTCGTGATGATCGTTATTTTGGTGTGGATg                           <  2:2418721/100‑1 (MQ=255)

                                                                            aCAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGt                         >  2:1199476/1‑100 (MQ=255)

                                                                            aCAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGt                         >  2:1981217/1‑100 (MQ=255)

                                                                             cAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGTATGGTa                        >  1:2913700/1‑100 (MQ=255)

                                                                               aGGCTACGATCCCAGCGAATTTACATTCGCTAACGGGGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAg                      >  2:2736258/1‑100 (MQ=255)

                                                                                ggCTACGATCCCAGCGAATTTACATTCGCTAAGGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGc                     <  1:532293/100‑1 (MQ=255)

                                                                                 cttccGATCTCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                    <  2:3186287/96‑1 (MQ=255)

                                                                                     cGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGt                >  2:1080948/1‑100 (MQ=255)

                                                                                      gATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTg               >  1:3341400/1‑100 (MQ=255)

                                                                                      accgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTg               >  2:25724/7‑100 (MQ=255)

                                                                                       ccgttaGCGAATGTAAATTCGCTGACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              >  2:1964055/6‑100 (MQ=255)

                                                                                       ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              >  2:2398183/6‑100 (MQ=255)

                                                                                       ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              >  1:150191/6‑100 (MQ=255)

                                                                                       ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              >  2:2512007/6‑100 (MQ=255)

                                                                                       ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              >  1:1129514/6‑100 (MQ=255)

                                                                                       ccgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTg               >  1:1697959/6‑99 (MQ=255)

                                                                                       aTCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              <  1:2155403/100‑1 (MQ=255)

                                                                                       aTCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTg               <  1:2464224/99‑1 (MQ=255)

                                                                                        tCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGa             <  1:1199559/100‑1 (MQ=255)

                                                                                        cgttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTgg              >  2:3800924/5‑99 (MQ=255)

                                                                                         gttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaa            >  1:3481101/4‑100 (MQ=255)

                                                                                         gttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaa            >  2:2235783/4‑100 (MQ=255)

                                                                                          ccAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaa           <  1:3047678/100‑1 (MQ=255)

                                                                                          ccAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTAGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaa            >  2:917588/1‑99 (MQ=255)

                                                                                           cAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCgagatcggaag          >  1:3186318/1‑90 (MQ=255)

                                                                                           cAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTAGTGTGGATGGTAAGAGGAGTGGaaaa          >  1:3768836/1‑100 (MQ=255)

                                                                                            aGCGAATTTACATTCGCTAACGTTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaaaa         <  2:1110437/100‑1 (MQ=255)

                                                                                            aGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTTGTAATGATCGTTATTATGTTATGAATGGTAAGCAGAGTGGaaaaa         >  1:674691/1‑100 (MQ=255)

                                                                                            aGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaaaa         >  2:1534004/1‑100 (MQ=255)

                                                                                             gCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaat        >  1:273736/1‑100 (MQ=255)

                                                                                             gCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaat        >  2:945615/1‑100 (MQ=255)

                                                                                                aaTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaa     <  1:3059564/100‑1 (MQ=255)

                                                                                                 aTTTACATTCGCTAACGGTGTATTTTGCGCTGCTAAAGCAAAATTATGTCGTGATGCTCGTTATTTTGGTGTGGCTGGTAAGCGAAGTGGAAAaatcaat    <  2:2126038/100‑1 (MQ=255)

                                                                                                 aTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGTAGTGGAAAaatcaat    >  1:3862828/1‑100 (MQ=255)

                                                                                                 aTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaat    <  1:95832/100‑1 (MQ=255)

                                                                                                 aTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaat    <  1:2786574/100‑1 (MQ=255)

                                                                                                 aTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatcaa     <  1:273196/99‑1 (MQ=255)

                                                                                                   ttACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAatcaatca  <  2:1641731/100‑1 (MQ=255)

TGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCA  >  NC_000913/1226733‑1226931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`