BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,246,073	C→T	100%	G435D (GGC→GAC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,073	0	C	T	100.0%	343.3 / NA	104	G435D (GGC→GAC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (67/37); total (67/37)

Only 100 of 107 total aligned reads displayed.

AGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCG  >  NC_000913/1245978‑1246169

aGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgtcg                                                                                                >  2:2901826/1‑98 (MQ=255)

 gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTACCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                             >  1:433744/1‑100 (MQ=255)

 gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGAGCATCCCATTGTTGCCCAATTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                             >  1:1188657/1‑100 (MQ=255)

  ttttGTAATCCTTCTGTAGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCgg                                                                                            >  2:1802582/1‑100 (MQ=255)

   tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                           >  1:1569917/1‑100 (MQ=255)

   tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                           >  1:1678576/1‑100 (MQ=255)

     tGTAATCCTTCTGTCGCGACCCACTGTAACTGTGCCCAGCCATCTGGCGCATCCCATTGTTGCCCACTTTTCACCTTCGTGGTGTTCAGGTGGCCGGGtt                                                                                         <  2:2062788/100‑1 (MQ=255)

       tAATCCTTCTGTCGCGACCCACTGTAACGGTGCTCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                       <  1:2084743/100‑1 (MQ=255)

       tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                       >  1:3472597/1‑100 (MQ=255)

         aTCCTTCTTTCGCGACCCACTGTAACGGTGCCCAGCTATTTGGCGCATCCCATTGTTGCACACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgca                                                                                      <  1:1724794/99‑1 (MQ=255)

         aTCCTTCTGTCGCGACCCACTGTAACGCTGCCCAGCCATTTGGCGCATCCCAGTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcag                                                                                     <  2:132239/100‑1 (MQ=255)

          tCCTTCTGTCGCGACCCACTGTATCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcag                                                                                     <  1:1053618/99‑1 (MQ=255)

          tCCTTCTGTCGCGACCCACTGTAACGGTGCCCACCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagc                                                                                    >  2:2350930/1‑100 (MQ=255)

            cTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGcagcag                                                                                  >  2:2111628/1‑100 (MQ=255)

            cTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGcagcag                                                                                  >  2:1676485/1‑100 (MQ=255)

            cTTCTGTCGCGACCCACTGTAACGGTGACCAGCCATTTGGCGCATCCAATTGTTGGACACTGTTAACCGACGTGGTGTTCAGGTAGCCGGGTTg                                                                                        >  2:3423876/1‑94 (MQ=255)

              tCTGTCGCGACCCACTGTAACGGTGCGCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAt                                                                                <  1:1911817/100‑1 (MQ=255)

              tCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAt                                                                                <  2:1816961/100‑1 (MQ=255)

               cTGTCGCGACCCACTGTAACGATGCCCAGCCATTTGGCACATCCCATTGTTGCCCCCTTTTCACCGACGTCGTGTTCAGGTCGCCGGGTTGCAGCAGa                                                                                 >  2:1723468/1‑98 (MQ=255)

                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                             <  1:3233918/100‑1 (MQ=255)

                 gTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGAAATCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                             >  1:1326825/1‑100 (MQ=255)

                   cgcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGtt                                                                           >  2:2475162/1‑100 (MQ=255)

                   cgcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGtt                                                                           >  2:3873959/1‑100 (MQ=255)

                    gcgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGttt                                                                          >  1:2997608/1‑100 (MQ=255)

                      gACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtc                                                                        >  1:3717717/1‑100 (MQ=255)

                       aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                       <  1:1500783/100‑1 (MQ=255)

                       aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                       <  1:2901852/100‑1 (MQ=255)

                       aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                       <  1:1499305/100‑1 (MQ=255)

                        cccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgt                                                                      >  1:3664825/1‑100 (MQ=255)

                        cccACTGTAACGGTGACCAGCCAATTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAAGTCGCCGGGTTGCAGCAGATGTGTTTtcgt                                                                      >  2:5416/1‑100 (MQ=255)

                         ccACTGTAGCGGGGCCCAGTCATGTGGCGCATCCCATTGTTGCCCACTTTTCACCGCCGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtgtg                                                                             <  2:1326203/92‑1 (MQ=255)

                         ccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                                                     >  2:1971538/1‑100 (MQ=255)

                          cACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                                    >  2:162458/1‑100 (MQ=255)

                          cACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                                    >  2:2594861/1‑100 (MQ=255)

                          cACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAAGTCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                                    >  1:745074/1‑100 (MQ=255)

                            cTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAACGACGTGGTGTTCAGGTAGCCGGGTTGCAGAAGATGTGTTTTcgtcgcc                                                                  >  1:1100484/1‑100 (MQ=255)

                              gTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                                >  2:318152/1‑100 (MQ=255)

                              gTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTAGTATTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                                >  1:409618/1‑100 (MQ=255)

                                ctcGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcg                                                              <  1:2195657/98‑1 (MQ=255)

                                aaCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcg                                                              <  1:1676500/100‑1 (MQ=255)

                                  cGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtcgag                                                            >  2:2195637/1‑98 (MQ=255)

                                  cGGTGCCCAGCCATTTGGCGCATCCAATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCgtcgccgtcgcc                                                            >  2:255121/1‑100 (MQ=255)

                                   ggTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCgtcgccgtcgcc                                                            <  1:2769911/99‑1 (MQ=255)

                                    gTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAt                                                          <  1:3549144/100‑1 (MQ=255)

                                    gTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAt                                                          <  1:3873998/100‑1 (MQ=255)

                                    gTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAt                                                          >  1:3037120/1‑100 (MQ=255)

                                     tGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtt                                                         >  2:2200782/1‑100 (MQ=255)

                                      gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                        >  2:2104578/1‑100 (MQ=255)

                                       cccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                                       >  1:383486/1‑100 (MQ=255)

                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGTTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                      <  2:49360/100‑1 (MQ=255)

                                        ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                      <  2:1256079/100‑1 (MQ=255)

                                           gCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgttg                                                   <  1:1978588/100‑1 (MQ=255)

                                             cATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgc                                                 >  2:268644/1‑100 (MQ=255)

                                              aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                                >  1:3415523/1‑100 (MQ=255)

                                              aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                                >  1:3415407/1‑100 (MQ=255)

                                                ttGGCGCATCCCATTGTTGCCCACTTTTCACCGTCGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGcgcg                                              <  1:211821/100‑1 (MQ=255)

                                                 tGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGa                                             <  2:1857164/100‑1 (MQ=255)

                                                  ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                            >  1:3634812/1‑100 (MQ=255)

                                                  ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                            >  1:3892053/1‑100 (MQ=255)

                                                  ggCACATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                            >  1:3337677/1‑100 (MQ=255)

                                                        tCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                                      <  1:1736279/100‑1 (MQ=255)

                                                         cccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                                     <  1:2320165/100‑1 (MQ=255)

                                                          ccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCACCATTTTGTTGGCGCGATCTTTCGCt                                    >  1:1909296/1‑100 (MQ=255)

                                                           cATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGCGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                                   >  2:1920662/1‑100 (MQ=255)

                                                            aTTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGc                                  <  1:2564708/100‑1 (MQ=255)

                                                             ttgttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGCGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGcc                                 <  1:1920678/100‑1 (MQ=255)

                                                                ttgCCCACTTTTCACCGACGTAGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                              <  2:2592094/100‑1 (MQ=255)

                                                                 tgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                             >  1:3087514/1‑100 (MQ=255)

                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                            >  1:1034146/1‑100 (MQ=255)

                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                            >  1:3578335/1‑100 (MQ=255)

                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                            >  2:1567953/1‑100 (MQ=255)

                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAtt                            >  1:938382/1‑100 (MQ=255)

                                                                  gCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTAGCCATTTTGTTGGAGCGATCTTTCGCTGCCGCAtt                            >  1:559934/1‑100 (MQ=255)

                                                                   cccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGGTGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGAGATCTTTCGATGCCGCATTg                           >  1:911296/1‑100 (MQ=255)

                                                                    ccACTTTTCACCGACGTGGTGTTCAGTTCGCCGGTTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTg                           <  1:1141383/99‑1 (MQ=255)

                                                                    ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                          >  2:1420480/1‑100 (MQ=255)

                                                                      aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGTCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                          <  2:2126126/98‑1 (MQ=255)

                                                                       cTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGAc                         <  2:1721044/98‑1 (MQ=255)

                                                                       cTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGt                       >  1:1322258/1‑100 (MQ=255)

                                                                        ttttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTa                      >  2:1836826/1‑100 (MQ=255)

                                                                           tCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAg                   >  2:3790971/1‑100 (MQ=255)

                                                                           tCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAg                   >  1:3231716/1‑100 (MQ=255)

                                                                           tCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAg                   >  2:85532/1‑100 (MQ=255)

                                                                             aCCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCg                 >  1:3852412/1‑100 (MQ=255)

                                                                             aCCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTAGTCGCCGTCGCCATTTTGTTGGCGCGTTCTTTCGCTGCCGCATTGACGTACAGc                  >  2:1774310/1‑99 (MQ=255)

                                                                               cGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCggg               <  1:2728215/100‑1 (MQ=255)

                                                                                gACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGa              >  1:2480341/1‑100 (MQ=255)

                                                                                    tggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg          >  2:3021850/1‑100 (MQ=255)

                                                                                     ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAACTTTCGCTGCCGCATTGACGTACAGAGGGAACAgg         >  1:1843300/1‑100 (MQ=255)

                                                                                     ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGACGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg         >  1:1455511/1‑100 (MQ=255)

                                                                                     ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGACGCCATTTTGTTGGCGCGGTGTTTTGCTGGGGGATTGACGTATAGTGGGaa             >  2:3862571/1‑96 (MQ=255)

                                                                                      gtgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTATTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAggg        >  1:2148820/1‑100 (MQ=255)

                                                                                       tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc       >  1:803718/1‑100 (MQ=255)

                                                                                       tgtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc       >  2:1908661/1‑100 (MQ=255)

                                                                                        gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg      <  2:3032701/100‑1 (MQ=255)

                                                                                          tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcggc    <  2:3593401/100‑1 (MQ=255)

                                                                                          tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcgac    <  1:2651406/100‑3 (MQ=255)

                                                                                           cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc   >  2:3382368/1‑100 (MQ=255)

                                                                                            aGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCcg  >  1:2473603/1‑100 (MQ=255)

                                                                                            aGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCAATCTTTCGCTGCAACATTGACGTACAGCGGGAACAGCGCGGCcg  >  1:910/1‑100 (MQ=255)

AGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCG  >  NC_000913/1245978‑1246169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`