Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,285,193 T→A 15.6% W19R (TGG→AGG)  narJ → molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,285,1930TA15.6% 395.5 / 58.6 160W19R (TGG→AGG) narJmolybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
Reads supporting (aligned to +/- strand):  ref base T (74/61);  new base A (18/7);  total (92/68)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 161 total aligned reads displayed.

CGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACG  >  NC_000913/1285095‑1285291
                                                                                                  |                                                                                                   
   aTCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgca                                                                                                 <  2:3480468/100‑1 (MQ=255)
   aTCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgca                                                                                                 >  2:2842201/1‑100 (MQ=255)
    tCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgcag                                                                                                >  2:1797009/1‑100 (MQ=255)
     cGATGCCATCGATGTGACGAGCAAAACGGGGCCGCGTCCATGATCGAATTCGTGTTTGTATCGCGCCTCCTTGAATATCCAGATGCTGCCTTATGgcagc                                                                                               <  2:2189796/100‑1 (MQ=255)
     cGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgcagc                                                                                               >  1:3235065/1‑100 (MQ=255)
       aTGCCATCGATGTGACCAGCAAATCGGAGTCGCATCCATGATCGATCTCGTGATTGTATCGCGTCTCCTTGATTTTCCGTATGCTGCCTTATGGCAGCAt                                                                                             <  2:505936/100‑1 (MQ=255)
        tGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATc                                                                                            <  2:3189543/100‑1 (MQ=255)
        tGCCATCGATGTGACCAGCAAAAAGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATc                                                                                            >  2:1581662/1‑100 (MQ=255)
         gCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCGTATGGCAGCATca                                                                                           <  1:2630370/100‑1 (MQ=255)
          ccATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTTAATATCCGGATGCTGCCATAAGGCAGCATccg                                                                                          <  2:1353353/100‑3 (MQ=255)
          ccATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCTTATGCTGCCATAAGGCAGCATccg                                                                                          <  2:1829290/100‑3 (MQ=255)
          ccATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATccg                                                                                          <  1:2107261/100‑3 (MQ=255)
            aTCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATcaaca                                                                                        >  1:3415629/1‑100 (MQ=255)
               gATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAaga                                                                                     >  2:830577/1‑100 (MQ=255)
               gATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAaga                                                                                     >  2:3378111/1‑100 (MQ=255)
                aTGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAagag                                                                                    >  2:2881204/1‑100 (MQ=255)
                  gtgACCAGCAAAACGGAGCAGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGCATATCCGGATGCTGCCTTATGGCAGCATCAACAagag                                                                                    >  2:2532467/1‑98 (MQ=255)
                   tgACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATg                                                                                 >  1:901850/1‑100 (MQ=255)
                   tgACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATg                                                                                 <  2:333597/100‑1 (MQ=255)
                    gACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGt                                                                                >  1:1807227/1‑100 (MQ=255)
                    gACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATAGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATg                                                                                 >  2:529541/1‑99 (MQ=255)
                     aCCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGACTGTATCGCGTCTCCTTGAACATACGGACGCTGCCTAATGGCAGCATCAACAAaaga                                                                                   >  2:3436636/1‑96 (MQ=255)
                      ccAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGttt                                                                              <  2:3595135/100‑1 (MQ=255)
                      ccAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATACCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGtt                                                                               >  1:892477/1‑99 (MQ=255)
                       cAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTg                                                                             <  1:3472558/100‑1 (MQ=255)
                       cAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTg                                                                             >  1:2026338/1‑100 (MQ=255)
                           aaaaCGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGc                                                                         >  1:3624975/1‑100 (MQ=255)
                               cGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATACTGCCTTATAACAACATCAACAAGAGATGTTTGAGGCGAtt                                                                     >  1:302736/1‑100 (MQ=255)
                                   gCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCcg                                                                 >  1:251963/1‑100 (MQ=255)
                                   gCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCcg                                                                 >  2:442532/1‑100 (MQ=255)
                                   gCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCcg                                                                 >  1:3600685/1‑100 (MQ=255)
                                    ccGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACCAGAGATGTTTGAGGCGATTg                                                                    <  2:2332855/96‑1 (MQ=255)
                                     cGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCcgcg                                                               <  1:1914402/100‑1 (MQ=255)
                                       cATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTc                                                             <  1:3884275/100‑1 (MQ=255)
                                       cATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTc                                                             <  2:2242217/100‑1 (MQ=255)
                                        aTCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCg                                                            <  1:2228244/100‑1 (MQ=255)
                                         tCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGa                                                           >  1:1522638/1‑100 (MQ=255)
                                          ccATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGaa                                                          >  2:1124232/1‑100 (MQ=255)
                                          ccATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGaa                                                          >  1:2288962/1‑100 (MQ=255)
                                            aTGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGaaaa                                                        >  2:1003804/1‑100 (MQ=255)
                                             tGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGaaaaa                                                       >  1:1648205/1‑100 (MQ=255)
                                              gATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAAt                                                      >  2:2564150/1‑100 (MQ=255)
                                              gATCGAACGCGTGTTTGTATCGCGTCTCCTTGAATATCCGTATGCTGCCTTATGGCATCATCAACAAGTTATGTTTGAGGCGATTGCCGCGTCGaa                                                          <  2:1058753/96‑1 (MQ=255)
                                                 cGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTg                                                   <  1:2118395/100‑1 (MQ=255)
                                                  gAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGc                                                  >  2:2979726/1‑100 (MQ=255)
                                                  gAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGc                                                  >  1:783921/1‑100 (MQ=255)
                                                  gAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAAAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTg                                                   >  1:1797215/1‑99 (MQ=255)
                                                   aaCTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGcc                                                 <  2:3235034/100‑1 (MQ=255)
                                                    aCTCGTGATTGTATCGCGTCTCCTTGAATATCCGTATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCa                                                <  2:444004/100‑1 (MQ=255)
                                                     cTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGCGATGTTTGAGGCGTTTGCCGCGTAGAAAAATTTGCCa                                                >  1:1618985/1‑99 (MQ=255)
                                                     cTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCaa                                               >  1:1851533/1‑100 (MQ=255)
                                                          gATTGTATCGCGTCTCCTTGAATATTCGGATTCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAagag                                          <  1:1402944/100‑1 (MQ=255)
                                                          gATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAagag                                          >  2:3619587/1‑100 (MQ=255)
                                                                aacgcgTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCTAAAAGAGGATGcc                                    <  2:1870701/98‑1 (MQ=255)
                                                                  cgcgTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCa                                  >  2:3749277/1‑100 (MQ=255)
                                                                    cgTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGTCAAAAGAGGATGCCCa                                  <  2:2485622/98‑1 (MQ=255)
                                                                         ccgttaaTATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATTTTTGAGGCGATTGCCACGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTg                          <  2:1749568/95‑1 (MQ=255)
                                                                         ccTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCt                           >  2:1217742/1‑99 (MQ=255)
                                                                         ccTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTg                          <  1:2357917/100‑1 (MQ=255)
                                                                         ccTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCACGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTg                          >  1:1454372/1‑100 (MQ=255)
                                                                          cTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                         >  2:3556995/1‑100 (MQ=255)
                                                                          cTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                         <  2:3229176/100‑1 (MQ=255)
                                                                          cTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                         >  1:3456852/1‑100 (MQ=255)
                                                                           ttGAATTTTCGGATGTTGCCTTATGGCAGCATCAACACGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                         <  1:2688904/99‑1 (MQ=255)
                                                                           ttGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAGAAATCTGCCAAAAGAGGATGCCCATGCGCTggg                        >  2:2805120/1‑100 (MQ=255)
                                                                           ttGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTggg                        <  2:142043/100‑1 (MQ=255)
                                                                           ttGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTggg                        <  1:975186/100‑1 (MQ=255)
                                                                           ttGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTggg                        <  1:762056/100‑1 (MQ=255)
                                                                            tGAATATCCGGATGCTGCCTTATGGAAGCATCAAGAATAGGTGTTTGAGGCGATTGCCGCGTCAAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGc                       <  2:415672/100‑1 (MQ=255)
                                                                             gAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCa                      <  1:2842227/100‑1 (MQ=255)
                                                                               atatCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGACGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCAtt                    >  1:168648/1‑100 (MQ=255)
                                                                                tatCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCAttt                   >  1:219532/1‑100 (MQ=255)
                                                                                 ttgttgATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCAtttt                  >  1:1045558/6‑100 (MQ=39)
                                                                                  tgttgATGCTGCCATAAGGCAGCATCCACAAGAGATGTTTGAGGCGATTGCCGCATCGAAAAATCTGCCAAAAGAGGATGCACATACGCTGGGCAttt                   >  1:631715/5‑98 (MQ=37)
                                                                                  tgttgATGCTGCCATAAGGCAGCATCAACAAGAGTTGTTTGAGGTGATTGTTGTGTCGAAAAATATGTTAAAAGAGGATGCCCATGCGCTGGGCAtttt                  >  1:3262525/5‑99 (MQ=25)
                                                                                  tCCGGATGCTGCCTTATGGTAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCAtttt                  <  1:940832/99‑1 (MQ=255)
                                                                                  tCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCAGCGCATGGGCAtcctc                 <  2:699720/100‑5 (MQ=255)
                                                                                   gttgATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTcc                >  2:632172/4‑100 (MQ=255)
                                                                                   gttgATGCTGCCATAAGGCACCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTcc                >  1:3694385/4‑100 (MQ=255)
                                                                                   ccGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCAGCGCATGGGCAtcctct                <  1:1808509/100‑6 (MQ=255)
                                                                                   ccGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCAGCGCATGGGCAtcctct                <  1:3669979/100‑6 (MQ=255)
                                                                                    ttgATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCt               >  1:118155/3‑100 (MQ=255)
                                                                                     tgATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTg              >  1:3728311/2‑100 (MQ=255)
                                                                                      gATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTgc             >  1:639590/1‑100 (MQ=255)
                                                                                      gATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTgc             >  2:156492/1‑100 (MQ=255)
                                                                                      gATGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTgc             >  1:1665165/1‑100 (MQ=255)
                                                                                       aTGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTgcg            <  2:490958/100‑1 (MQ=255)
                                                                                       aTGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTgcg            <  1:2980541/100‑1 (MQ=255)
                                                                                       aTGCTGCCATAAGGCAGCATCAACAAGCGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAAAGGATGCCAATGCACTGGGCATTTTCCTgcg            >  1:2467660/1‑100 (MQ=255)
                                                                                        tgctgcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGTCACAAGAGGATGCCCATGCGCTGGCCATTTTCCTgcgc           <  2:2413035/100‑1 (MQ=255)
                                                                                        tgctgcCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTgcgc           >  1:1365199/1‑100 (MQ=255)
                                                                                          ctgcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGa         >  2:1401361/1‑100 (MQ=255)
                                                                                          ctgcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGa         >  2:3300931/1‑100 (MQ=255)
                                                                                          ctgcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGa         >  2:1724478/1‑100 (MQ=255)
                                                                                           tgcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAt        <  1:1473204/100‑1 (MQ=255)
                                                                                           tgcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAt        <  2:1772460/100‑1 (MQ=255)
                                                                                            gcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAtt       >  2:1111558/1‑100 (MQ=255)
                                                                                             ccTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAttt      >  2:2872652/1‑100 (MQ=255)
                                                                                               ttATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTaa    <  1:715370/100‑1 (MQ=255)
                                                                                               ttATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTaa    >  2:3711963/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACG  >  NC_000913/1285095‑1285291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: