Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,835 A→T 16.3% intergenic (‑92/+19) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8350AT16.3% 339.1 / 54.6 137intergenic (‑92/+19)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (56/58);  new base T (8/14);  total (64/73)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-02

Only 100 of 142 total aligned reads displayed.

TTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAG  >  NC_000913/1441736‑1441934
                                                                                                   |                                                                                                   
ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                     <  1:2026978/100‑1 (MQ=255)
 tcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAt                                                                                                    <  2:1913109/100‑1 (MQ=255)
  cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATg                                                                                                   >  2:849344/1‑100 (MQ=255)
   gtcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGc                                                                                                  >  1:3460470/2‑100 (MQ=255)
     cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGAAATGCAg                                                                                                >  1:3521484/1‑100 (MQ=255)
      aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                               >  1:3223805/1‑100 (MQ=255)
      aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                               <  1:248473/100‑1 (MQ=255)
        tttgtgtcttAATTACAGTTTCTGGTTCAGGACTATTTTAAGCATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                             <  2:3834650/94‑1 (MQ=255)
        tgtgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                               >  2:3669707/1‑98 (MQ=255)
         gtgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGa                                                                                            >  1:771735/1‑100 (MQ=255)
          tgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAg                                                                                           <  1:1501280/100‑1 (MQ=255)
           ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                          <  2:108685/100‑1 (MQ=255)
            gTTCTCCATTACAGTTTCTTACTCAGTACTATTTTCAGTATATAGGATTACAGGTCATTGAGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                          <  2:3319124/99‑1 (MQ=255)
            gTTCTCAATTTAAGTTTCTCACTCAGTTCTATTTTACGAATTGTGGATGAAAGGTCATTGGGGATTATCTGAACCAGCTCCCCTGCATTCCAGGGGAGct                                                                                         <  1:2280261/100‑2 (MQ=255)
              tctcAATTACAGTTTCTGACTCAGGACTATTTTAATAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTTCCCTGCATTCCAGGGGAGctga                                                                                       <  2:672643/100‑4 (MQ=255)
              tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctga                                                                                       <  2:1797048/100‑4 (MQ=38)
                tcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCTGGGGAGc                                                                                          >  2:3707223/1‑95 (MQ=255)
                tcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTAGGGATTATCTGAATCAGCTCACCTGGAATGCAGGGGAGCGGCaa                                                                                     >  1:1600123/1‑100 (MQ=255)
                  aaTTATAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                   <  1:1690455/100‑1 (MQ=255)
                  aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                   <  1:1198113/100‑1 (MQ=255)
                  aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattca                                                                                   <  2:2955368/100‑8 (MQ=38)
                     tACAGTTTCTGTCTCATGACTATTTTAAGAATAGAGTATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattcagat                                                                                <  1:3569862/100‑11 (MQ=21)
                     tACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattcagat                                                                                <  1:3494053/100‑11 (MQ=37)
                     tACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGAGATTATCTGAATCAGCTCCACTGGAATGCAGGGGAGCGGCAAGATTa                                                                                >  2:407556/1‑100 (MQ=255)
                      aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTACCCTGGAATGCAGGGGAGCGGCAAGATTaa                                                                               >  1:2703369/1‑100 (MQ=255)
                      aCAGTGTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                    <  2:2344883/95‑1 (MQ=255)
                       cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGTATGATAGGTTATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                              <  1:1223077/100‑1 (MQ=255)
                        aGTTTCTGAGTCAAGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCCCCGCTGGAATGCAGGGGAGCGGCAAGATTaaag                                                                             <  2:2568179/100‑2 (MQ=255)
                         gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCCTTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                            >  2:454495/1‑100 (MQ=255)
                         gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                            <  1:2193572/100‑1 (MQ=255)
                           ttCTGACTCATGACTATTTTAAGAATAGAGGATGAAAGTTCATTGGGTATTATCTGAATCAGCTCCCCTTGAATTCAGGTTAGCGGCAAGATTAAACCAg                                                                          <  2:1886095/100‑1 (MQ=255)
                             cTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                        <  1:3013611/100‑1 (MQ=255)
                               gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                      >  2:1024623/1‑100 (MQ=255)
                                   cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                  <  1:1963811/100‑1 (MQ=255)
                                      gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcgcg                                                                <  1:3632848/99‑3 (MQ=255)
                                       aCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCa                                                              >  1:2349937/1‑100 (MQ=255)
                                          aTTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGt                                                           >  1:3212089/1‑100 (MQ=255)
                                           ttttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCACCACTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGt                                                           >  2:3436378/1‑99 (MQ=255)
                                            tttAAGAATAGAGGATGAAAGGTCATTGAGGATTATATGAATCAGCTCACCTGGAATGCAGGGGATAGGCAAGATTAAACCAATTCGTTCGGGCa                                                              >  2:1935122/1‑95 (MQ=255)
                                              tAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCg                                                       >  2:581430/1‑100 (MQ=255)
                                              tAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCg                                                       <  2:3701127/100‑1 (MQ=255)
                                               aaGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                      <  1:688129/100‑1 (MQ=255)
                                                aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                     >  2:3354802/1‑100 (MQ=255)
                                                aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                     <  1:1516276/100‑1 (MQ=255)
                                                aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                     <  1:3116378/100‑1 (MQ=255)
                                                 gAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCt                                                    <  1:1623964/100‑1 (MQ=255)
                                                   aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttt                                                  >  1:1290091/1‑100 (MQ=255)
                                                    tAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACAAGTTCGTTCGGGCAGGTTTCGCCtttt                                                 >  1:753440/1‑100 (MQ=255)
                                                      gagGATTAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                     <  2:1178633/94‑1 (MQ=255)
                                                       agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                              <  2:515566/100‑1 (MQ=255)
                                                        ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTc                                               >  2:142804/1‑98 (MQ=255)
                                                        ggATGAAAGGTCATTGGAGATTATCTAAATCAACTCCCCTGGAATGCAAGAAAACGACAAAATTAAACCAGTTCGTTCGAGCAAATTTAGCCTTTTTCCa                                             >  2:2305686/1‑100 (MQ=255)
                                                         gATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                            <  1:3707911/100‑1 (MQ=255)
                                                          attaaaGGTCATTGGGTATTATCTGAATTAGCTTCTCTGGTATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCa                                             <  1:1724202/95‑1 (MQ=255)
                                                           tGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAt                                          >  2:3513409/1‑100 (MQ=255)
                                                           tGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAt                                          >  1:955044/1‑100 (MQ=255)
                                                             aaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTg                                        >  2:760573/1‑100 (MQ=255)
                                                              aaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAttcc                                       <  1:2650040/100‑3 (MQ=255)
                                                                    aTTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGt                                 >  1:1015629/1‑100 (MQ=255)
                                                                      tGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGttt                               <  2:3831977/100‑1 (MQ=255)
                                                                       ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAg                                  >  1:3347888/1‑96 (MQ=255)
                                                                       ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                              <  1:2556629/100‑1 (MQ=255)
                                                                       ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAAAAGTTAGTTAGGGAAGGTTTAGCCTTTTTAAAGATTGCTTAAGtttt                              >  1:2250352/1‑100 (MQ=255)
                                                                           aTTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAg                          <  1:3327154/100‑1 (MQ=255)
                                                                           aTTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAg                          >  2:1385351/1‑100 (MQ=255)
                                                                            ttATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGc                         >  2:3383833/1‑100 (MQ=255)
                                                                             tATCTGAATCAGCTCCCCTGGAATGTAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCg                        <  1:3520648/100‑1 (MQ=255)
                                                                             tATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCg                        <  1:1260270/100‑1 (MQ=255)
                                                                              taatcttgccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                       >  1:724979/11‑100 (MQ=37)
                                                                              aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                       >  1:2690256/1‑100 (MQ=255)
                                                                               tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                       >  1:2897612/1‑99 (MQ=255)
                                                                                cTGAATCAGCTCCCCTGGAATGCAGGGGAGCTGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                     <  1:2207750/100‑1 (MQ=255)
                                                                                 tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                    >  1:191246/1‑100 (MQ=255)
                                                                                    aTCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                 <  2:1290077/100‑1 (MQ=255)
                                                                                     tCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTga                <  1:1589325/100‑1 (MQ=255)
                                                                                      ctgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               <  1:2420411/98‑1 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:3578662/3‑100 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:1733682/3‑100 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:625322/3‑100 (MQ=255)
                                                                                      cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCTGGCATGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               <  1:1011908/100‑1 (MQ=255)
                                                                                      cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               <  1:3894187/100‑1 (MQ=255)
                                                                                       cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              >  1:1200336/2‑100 (MQ=255)
                                                                                       aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              <  2:2983081/100‑1 (MQ=255)
                                                                                        gCTCCCCTGGAATGCAGTGGAGCGGCAAGATTAAACAAGTTCGTTCGGGCAGGTTTCGCCTTTTTACAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa             >  2:716515/1‑100 (MQ=255)
                                                                                        gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgagag             >  2:2420389/1‑99 (MQ=255)
                                                                                        gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa             >  1:3374156/1‑100 (MQ=255)
                                                                                         cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa            <  2:3147521/100‑1 (MQ=255)
                                                                                         cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa            >  2:2601696/1‑100 (MQ=255)
                                                                                          tgcccggtaaTGGAGGGGTTCGGCAGGTTTAAAGGAGTTCGTTTGGGCAGGTTTCGCCTTGTTAAAGATTGCTTAAGTGTTGCAGCGTAGTCTgaga              <  2:1949265/89‑1 (MQ=255)
                                                                                          tCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTCCCAGACAGATTAAGTTTTGCAGCGTAGTCTGAGAAAt           >  1:661244/1‑100 (MQ=255)
                                                                                           ccccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCATGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATa          >  1:960032/1‑100 (MQ=255)
                                                                                           ccccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATa          >  1:87584/1‑100 (MQ=255)
                                                                                            cccTGGAATGCAGGGGGGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCACATTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTATGAGAAATAc         >  1:1077750/1‑100 (MQ=255)
                                                                                             ccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACt        <  2:2690232/100‑1 (MQ=255)
                                                                                             ccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACt        <  2:2972995/100‑1 (MQ=255)
                                                                                                ggAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGt     >  2:2475936/1‑100 (MQ=255)
                                                                                                 gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTc    >  1:568674/1‑100 (MQ=255)
                                                                                                  aaTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTACGTTTTGCAGCGTAGTCTGAGAAATACTGGTCa   <  2:422419/100‑1 (MQ=255)
                                                                                                   aTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCag  <  1:1659785/100‑1 (MQ=255)
                                                                                                   aTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCag  >  2:1477525/1‑100 (MQ=255)
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TTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAG  >  NC_000913/1441736‑1441934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: