Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,755,639	G→A	100%	intergenic (‑498/‑59)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,639	0	G	A	99.0%	302.3 / ‑4.7	96	intergenic (‑498/‑59)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (54/40);  total (56/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

CTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA  >  NC_000913/1755542‑1755735                                                                                                  |                                                                                                 cTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACa                                                                                                >  2:3264867/1‑100 (MQ=255)  tGAGACACAAGCACACATTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAg                                                                                               >  2:515791/1‑100 (MQ=255)  tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAg                                                                                               <  2:3101170/100‑1 (MQ=255)    agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTg                                                                                             <  2:1733410/100‑1 (MQ=255)     gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCTCCTCTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                            <  2:1406972/100‑1 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTTTATTTTTTTTTTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                          >  2:2732675/1‑100 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGCCAGTgcgc                                                                                          >  2:1446281/1‑100 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                          >  2:3338988/1‑100 (MQ=255)       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAAAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                          >  1:1666355/1‑100 (MQ=255)        acaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGcc                                                                                         <  1:194505/100‑1 (MQ=255)        acaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGcc                                                                                         <  1:2266982/100‑1 (MQ=255)         caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                        <  2:504627/100‑1 (MQ=255)         caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                        <  2:611787/100‑1 (MQ=255)          aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                       >  2:862277/1‑100 (MQ=255)           aGCACACATTCCTCTGCACGCTTTTTCGCTGTCACCTATCCTTAGAGCGAAGCACCACCACTATCGTAAAACCGGATTCGATTTCCGACAGTGCGCCAAg                                                                                      >  1:658434/1‑100 (MQ=255)           aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                      >  2:1568867/1‑100 (MQ=255)             cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGaa                                                                                    >  1:2803026/1‑100 (MQ=255)              acacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAAGCACCACCACTTTCGTAATACAGAATTCGCTTTCCGACAGTGCGCCCAGaaa                                                                                   >  1:202799/1‑100 (MQ=255)               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                  >  2:3602846/1‑100 (MQ=255)               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                  >  1:934920/1‑100 (MQ=255)               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                  >  2:243790/1‑100 (MQ=255)                acaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGATCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAATGCGCCCAGAAAg                                                                                  >  1:3009120/1‑99 (MQ=255)                 caTTCCTCTGGAGGCTTTTTAGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGAATTCGGTTTCCGACAGTGCGTCCAGGAAg                                                                                  >  2:2913907/1‑98 (MQ=255)                 caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCa                                                                                >  2:1906714/1‑100 (MQ=255)                     ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                            >  1:942206/1‑100 (MQ=255)                      ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGttt                                                                           >  1:3114084/1‑100 (MQ=255)                       tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGTACTATCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtc                                                                          <  1:1151809/100‑1 (MQ=255)                       tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCAGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtc                                                                          >  1:335574/1‑100 (MQ=255)                        ctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGAATTCAATTTCAAACAGAGCGCCCAGAAAGCAAGTTtct                                                                         >  1:697097/1‑100 (MQ=255)                         tGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtctc                                                                        <  2:758093/100‑1 (MQ=255)                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACCGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       >  2:1198752/1‑100 (MQ=255)                           cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                      >  1:3081835/1‑100 (MQ=255)                           cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCAGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                      >  1:1388999/1‑100 (MQ=255)                               cTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                  <  2:578108/100‑1 (MQ=255)                               cTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                  >  1:384481/1‑100 (MQ=255)                                tttttCGATGTCACCTATCCTTAGAGCGAGGTACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                 <  1:1519327/100‑1 (MQ=255)                                tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                 >  1:1481273/1‑100 (MQ=255)                                tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                 >  1:1481410/1‑100 (MQ=255)                                tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                 <  1:917717/100‑1 (MQ=255)                                   ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                              >  2:1418436/1‑100 (MQ=255)                                    tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc                                                             <  2:934909/100‑1 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGGACCACCACTTTCGTAATACCGGCTTCGCTTTCCGACAGTGCGCCCAGCAAGCAAGTTTCTCCCATCCTTCTCa                                                            >  2:2645962/1‑100 (MQ=255)                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCAAGAAAGCAAGATTCTCCCATCCTTCTCa                                                            >  1:2412281/1‑100 (MQ=255)                                     cGATGTCACCTAACCTGCGAGCGAGGCACCAACACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAAAAAGCAAGTTTCTCCCATCCTTCTCa                                                            <  2:261511/100‑1 (MQ=255)                                        tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                         >  1:786453/1‑100 (MQ=255)                                        tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                         <  1:3414237/100‑1 (MQ=255)                                         gTCACCTATCCTTAGAGCGAGGACACACCAACTTCGTAATACCGGATTCGCTTTTCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                         <  2:2330460/99‑1 (MQ=255)                                           cACCTATCCTTAGAGCGAGGCACCACTACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAATCAAGTTTCTCCCATCCTTCTCAACTTaa                                                      >  2:295718/1‑100 (MQ=255)                                            aCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCTACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaaa                                                     >  1:524590/1‑100 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    <  1:2552759/100‑1 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    >  1:2185244/1‑100 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    <  2:863393/100‑1 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    <  2:942195/100‑1 (MQ=255)                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCACAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    >  1:1371501/1‑100 (MQ=255)                                              cTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaaga                                                   <  2:3452247/100‑1 (MQ=255)                                              cTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaaga                                                   <  1:2623198/100‑1 (MQ=255)                                               tttCCTTAGAGCGAGGCACCACCACTTTCGTAATTCCTGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaaga                                                   <  2:1326627/97‑1 (MQ=255)                                               tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                  >  1:3437944/1‑100 (MQ=255)                                                aTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagact                                                 >  2:3480548/1‑100 (MQ=255)                                                  ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCACAGAAAGCAAGTTTATCCCATCCTTCTCAACTTAaagactaa                                               >  1:3610849/1‑100 (MQ=255)                                                    ttAGAGCGAGTCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                             <  2:197272/100‑1 (MQ=255)                                                    ttAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                             <  2:254101/100‑1 (MQ=255)                                                      agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaaga                                             >  2:120772/1‑98 (MQ=255)                                                         gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCATAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTc                                        <  2:2169851/100‑1 (MQ=255)                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                       <  1:1254118/100‑1 (MQ=255)                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                       <  2:1675904/100‑1 (MQ=255)                                                           gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                      >  1:74066/1‑100 (MQ=255)                                                            aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                     <  1:3330164/100‑1 (MQ=255)                                                             ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                    >  1:2267601/1‑100 (MQ=255)                                                                accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGTCTAAAAATGtaaaaaaaa                                 <  2:1146950/100‑9 (MQ=255)                                                                accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                 >  1:1871260/1‑100 (MQ=255)                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                               <  2:524583/100‑1 (MQ=255)                                                                   accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa                              >  2:2145387/1‑100 (MQ=255)                                                                   accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa                              >  2:1152228/1‑100 (MQ=255)                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                               <  1:1299713/98‑1 (MQ=255)                                                                     cacTTTCGTAATACCGGATTCTCTTTCCGACAGTGCTCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAATACTAAGAATGAAATGAAAAAGAcc                            <  2:1342410/100‑1 (MQ=255)                                                                       cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                          >  2:2425773/1‑100 (MQ=255)                                                                            gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGGCTGTCATGAAAAAGACCaaa                         >  2:2403035/1‑96 (MQ=255)                                                                             tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt                    >  2:3561965/1‑100 (MQ=255)                                                                             tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt                    >  1:2902244/1‑100 (MQ=255)                                                                              aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                   <  1:1960620/100‑1 (MQ=255)                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                  <  2:1567255/100‑1 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGTGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                 >  2:2238467/1‑100 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                 >  2:173394/1‑100 (MQ=255)                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                 <  2:642739/100‑1 (MQ=255)                                                                                 aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc                <  2:2808421/100‑1 (MQ=255)                                                                                  ccGGATTCGCTTTCCGACAGTGCGCACAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGAATAAGACTGTCATGAAAAAGACCACAATTGTTTGCa               >  2:845404/1‑100 (MQ=255)                                                                                       ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc          <  2:74063/100‑1 (MQ=255)                                                                                        tCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCg         <  2:2016746/100‑1 (MQ=255)                                                                                         cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg        >  2:2861375/1‑100 (MQ=255)                                                                                         cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg        >  1:3571075/1‑100 (MQ=255)                                                                                          gCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGa       <  2:1871245/100‑1 (MQ=255)                                                                                           cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGa       >  1:688851/1‑99 (MQ=255)                                                                                           cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      <  1:944770/100‑1 (MQ=255)                                                                                               ccGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGaa  >  1:896649/1‑100 (MQ=255)                                                                                                cGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACAATCGGACCGaa  >  1:3155063/1‑99 (MQ=255)                                                                                                  |                                                                                                 CTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA  >  NC_000913/1755542‑1755735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑