Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,400 G→A 16.9% R405W (CGG→TGG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,4000GA16.9% 358.6 / 54.3 161R405W (CGG→TGG) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (65/67);  new base A (12/15);  total (78/83)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-02

Only 100 of 164 total aligned reads displayed.

CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTT  >  NC_000913/2140301‑2140498
                                                                                                   |                                                                                                  
cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCg                                                                                                    <  2:3893832/100‑1 (MQ=255)
cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCg                                                                                                    >  1:2356499/1‑100 (MQ=255)
  gCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                                                  <  2:2726031/100‑1 (MQ=255)
  gCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                                                  <  2:1498307/100‑1 (MQ=255)
  gCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGAACCAATCTCAACGTTTTCCAGCCGTg                                                                                                  >  1:1714380/1‑100 (MQ=255)
     ccGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCt                                                                                               <  2:1512723/100‑1 (MQ=255)
       ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                                             >  2:1456204/1‑100 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGTCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                          <  2:2167295/100‑1 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTGAGAATGTTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                          <  1:2700287/100‑1 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                          <  2:3782515/100‑1 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                          <  1:2691386/100‑1 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                          <  1:580729/100‑1 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACTAAATCGTATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                         <  2:3912528/100‑3 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAgn                                                                                         <  2:2459775/100‑2 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGt                                                                                         <  2:809799/100‑1 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                         <  1:2866890/100‑3 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                         <  1:2257074/100‑3 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                         <  1:2133927/100‑3 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCTGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                        <  1:3399621/100‑4 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                        <  1:2587356/100‑4 (MQ=255)
             aTCACCATACAGTGACATTTTTCCGGTCAACGCAATCGGATAGTTAAACGCCTTCAGAATAGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                                                       <  2:1832905/100‑5 (MQ=255)
             aTCACCAGCCAGTGACATTTTTTTGTTTATTTAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAGCGCAACTTTTTCCAGCCCTTGCTGTAAGtt                                                                                        >  2:3325917/1‑99 (MQ=255)
              tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaactt                                                                                      <  1:3639001/100‑6 (MQ=255)
               cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCATAATGGTAACAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTAt                                                                                     >  2:3243982/1‑100 (MQ=255)
               cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgtt                                                                                     <  2:1971037/100‑7 (MQ=255)
                aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgttg                                                                                    <  1:1921868/100‑8 (MQ=255)
                  cagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcg                                                                                  >  2:310003/1‑100 (MQ=255)
                  cagccagTGACATTGTTCCTGTCATCGAATTTGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAATGTTTTCCTGCCACGGCTGGaaaacgtt                                                                                     <  2:1766202/97‑7 (MQ=255)
                    gccagTGACATTTTTCCGGTCAACTAAATCGGATAGTTTAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgcg                                                                                <  1:200071/100‑1 (MQ=255)
                    gccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgcg                                                                                <  1:2888356/100‑1 (MQ=255)
                    gccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgcg                                                                                <  1:3919884/100‑1 (MQ=255)
                      cagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGa                                                                              >  1:3052874/1‑100 (MQ=255)
                            cATTTTTCCGGTCATCGAAATCGGATAGTTAACCCCCTTCAGAATGGTCCCTATCTCAACGTTTTCCAGCCGTGGCTGTAAGTTTCTCCGCGGATTTAt                                                                         <  2:3765357/99‑1 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAACCGCCTTCAGAATGGTACCATTCTCAACGTTTTCCAGCCGTGTCTGTAAGTTTATCCGCGGATTTAt                                                                         >  2:3406807/1‑99 (MQ=255)
                              tttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGa                                                                      <  2:3042273/100‑1 (MQ=255)
                              tttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGa                                                                      >  2:3363174/1‑100 (MQ=255)
                               ttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGAt                                                                     <  2:831008/100‑1 (MQ=255)
                                tttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATg                                                                    >  1:253927/1‑100 (MQ=255)
                                    cGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcg                                                                 <  2:3464656/99‑1 (MQ=255)
                                     tgTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTACAGCCGTGGCTGGAAGTTTATCCGAGGATTTATTGATGTcgcg                                                               >  1:979098/2‑100 (MQ=255)
                                     ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                               >  2:3801243/1‑100 (MQ=255)
                                     ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                               >  1:979971/1‑100 (MQ=255)
                                     ggTCAACGAAATCGGATAGCTAAACGCCTTCAGACTGGTACCAATCTCAACGTTTTCCAGCCGTGGATGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                               >  2:802044/1‑100 (MQ=255)
                                      gTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                              <  2:2396718/100‑1 (MQ=255)
                                      gTCAACGAAATCGGATAGTTAAACGACTTCAGAATGGTGCCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATATcacg                                                               >  2:579338/1‑99 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                            <  2:15525/100‑1 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                            >  2:2107521/1‑100 (MQ=255)
                                        cAACGAAATCGGATAGATAAACGGCTTCAGAATCGTCCTAATCTCAACGTTTTCGAGCCGTGACTCGAAGTTTATCCGCGGATTTATTGATTTCGCGTcc                                                            >  2:2983144/1‑100 (MQ=255)
                                         aaCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCa                                                           >  1:3852926/1‑100 (MQ=255)
                                         aaCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCa                                                           >  2:1360660/1‑100 (MQ=255)
                                          aCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCa                                                           >  2:132921/1‑99 (MQ=255)
                                              aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                      <  2:513863/100‑1 (MQ=255)
                                                tCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGcc                                                    >  1:512086/1‑100 (MQ=255)
                                                 cGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGccc                                                   >  2:625413/1‑100 (MQ=255)
                                                 cGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGccc                                                   <  2:3747716/100‑1 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                  >  2:1580733/1‑100 (MQ=255)
                                                  ggATAGTTAAACGCATTCAGAATGGTACCAATCTCAAGGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTAGCGTCCAGCGt                                                       >  2:1147187/1‑95 (MQ=255)
                                                    aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCgg                                                 >  2:3229939/1‑99 (MQ=255)
                                                    aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCgg                                                 <  1:3444937/99‑1 (MQ=255)
                                                      aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                              <  1:2902913/100‑1 (MQ=255)
                                                      aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                              <  2:1854631/100‑1 (MQ=255)
                                                      aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                              <  1:1360675/100‑1 (MQ=255)
                                                       gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                             >  1:1752880/1‑100 (MQ=255)
                                                        ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTg                                            <  1:3341731/100‑1 (MQ=255)
                                                          aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                          <  1:2452069/100‑1 (MQ=255)
                                                          aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                          <  1:2304015/100‑1 (MQ=255)
                                                           aaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaaa                                         <  1:1401659/100‑1 (MQ=255)
                                                            aCGCCTTCAGAATGGTACCAATCTCAACTTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                        <  1:112945/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                       <  1:2379859/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                       <  1:625420/100‑1 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCGGCGTGCCCGGCAGTGAAACCTGt                                    >  1:813150/1‑100 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                    >  1:2511157/1‑100 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                    <  1:3181538/100‑1 (MQ=255)
                                                                    aGAATGTTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCAc                                <  1:2739934/100‑1 (MQ=255)
                                                                       acggTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACATGTCCACCg                              >  1:2636531/3‑99 (MQ=255)
                                                                        tGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAATAATGTCGAGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGtt                            >  1:3126129/1‑100 (MQ=255)
                                                                          ggaCCAATCTCAATGTTTTCCAGCCGTGGCTGGAAGTTTATCAGCGGATTTATTGATGTCGCGTCCAACGTGCCCGGCAGTGAAAACTGTCCACCGTTaa                          >  1:2650463/3‑100 (MQ=255)
                                                                              cAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGttt                      >  1:2238826/1‑100 (MQ=255)
                                                                              cAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGAGTGACAGGCAGTGAAACCTGTCCACCGTTAAGtt                       >  1:778394/1‑99 (MQ=255)
                                                                               aaTCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTg                     >  2:3642944/1‑100 (MQ=255)
                                                                                 tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATACGCTGATTTATTGATGTCGCGTCCAGCGTGACCGGCAGTGTAACCTGTCCACCGTCAAGTTTGcc                   >  2:498921/1‑100 (MQ=255)
                                                                                  ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCTTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAATTTGcc                   >  1:3326402/1‑99 (MQ=255)
                                                                                    cAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTTGCTTTCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTg                <  1:1059876/100‑1 (MQ=255)
                                                                                     aaCGTTTTCCAGCCGTGGCTGGAAGTTTATTCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc               <  1:309290/100‑1 (MQ=255)
                                                                                     aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc               <  1:1476176/100‑1 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCa              <  1:1214091/100‑1 (MQ=255)
                                                                                       cGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg             <  1:1986265/100‑1 (MQ=255)
                                                                                       aaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg             >  1:1374716/5‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAACGTGCCCGGCAGTGAAACCTGTCAACCGTTAAGTTTGCCCTGCAg             >  1:3430902/4‑99 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt           >  1:1069982/3‑100 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt           >  1:1261035/3‑100 (MQ=255)
                                                                                          tttCCAGCCTTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg          <  1:933260/100‑1 (MQ=255)
                                                                                          tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTTACGTCGCGTCCAGCGTGCCGGGCAGTGCAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg          <  2:2126582/100‑1 (MQ=255)
                                                                                          tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg          <  2:3087330/100‑1 (MQ=255)
                                                                                          cttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCAGGAAGTGAAACCTGTACACCGTTAAGTTTGCCCTGCAGTTg          >  1:3319041/2‑100 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg        >  1:726767/1‑100 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg        <  1:685728/100‑1 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg        >  1:1464229/1‑100 (MQ=255)
                                                                                             ccAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGt       <  1:523585/100‑1 (MQ=255)
                                                                                                  cGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAAttt  <  2:2796082/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTT  >  NC_000913/2140301‑2140498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: