Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,323,291 T→A 15.6% intergenic (+40/‑156) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,2910TA15.6% 368.1 / 47.9 139intergenic (+40/‑156)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (59/58);  new base A (16/6);  total (75/64)
Fisher's exact test for biased strand distribution p-value = 6.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

AGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGG  >  NC_000913/2323192‑2323388
                                                                                                   |                                                                                                 
aGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                   >  2:1995074/1‑100 (MQ=255)
aGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                   >  2:1109345/1‑100 (MQ=255)
aGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                   >  1:1835511/1‑100 (MQ=255)
aGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTACTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                   >  1:749554/1‑100 (MQ=255)
  tCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAttt                                                                                                 <  2:2252781/100‑1 (MQ=255)
    gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTc                                                                                               <  2:3094609/100‑1 (MQ=255)
      cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                             <  1:2441302/100‑1 (MQ=255)
      cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                             <  1:2408028/100‑1 (MQ=255)
       gTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGc                                                                                            <  1:971477/100‑1 (MQ=255)
        tgtaTTGATGTATAAATTCCAGGAATACCGTATCGATCCGGCGGATGTATAACACCAAAACTTTCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                             <  2:472193/95‑1 (MQ=255)
        tGCATTGATGTATACACTCCAGGAATACGGTATCGATTCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                             <  1:3527272/98‑1 (MQ=255)
          cATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATa                                                                                         <  1:1088308/100‑1 (MQ=25)
           aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGTCGGATGTATAACACCAAAACTTGCTATGCATAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                        <  2:408281/100‑1 (MQ=25)
           aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCGCAGTGCGCAATTTTCTGCATa                                                                                         >  2:3500097/1‑99 (MQ=255)
           aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAg                                                                                        <  1:2463196/100‑1 (MQ=255)
                tGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                   <  1:1609644/100‑1 (MQ=255)
                  tataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAAATTGATATACAGAAATTTGCACAGTGAGCAATTTTCTGCATAGCCGCTCa                                                                                 >  1:1947395/1‑100 (MQ=255)
                   ataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCAt                                                                                >  1:2360931/1‑100 (MQ=255)
                   ataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCAAAGTGCGAAATTTTCTGAATAGCCGCt                                                                                   >  1:2679845/1‑97 (MQ=255)
                       aCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtctc                                                                            <  1:2077792/100‑1 (MQ=255)
                       aCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtctc                                                                            <  1:2561192/100‑1 (MQ=255)
                        cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                           <  2:2537637/100‑1 (MQ=255)
                        cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                           <  1:780477/100‑1 (MQ=255)
                         tCCAGGAATACGGTATCGATCCGGCTGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCt                                                                          <  2:3716412/100‑1 (MQ=255)
                          ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCtt                                                                         >  1:124795/1‑100 (MQ=255)
                          ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCtt                                                                         >  1:1965002/1‑100 (MQ=255)
                          ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCt                                                                          <  2:101692/99‑1 (MQ=255)
                             ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                      <  1:3600996/100‑1 (MQ=255)
                             ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                      >  1:3176948/1‑100 (MQ=255)
                             ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                      <  1:3119013/100‑1 (MQ=255)
                             ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCACAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                      >  1:3269296/1‑100 (MQ=255)
                             ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGAAAAGTGCGCAATTTTCTGAATAGCCGCTAATTCTCATtata                                                                      >  1:2295878/1‑100 (MQ=255)
                               aaTACGGTATCGATCTGATGTATGTATTAAACATAATTTTGCTAGGCAGACATTTGCACATTGCTCTATTTTGTTTATAGCAACTCATTCACCTta                                                                        <  2:1874935/96‑1 (MQ=255)
                                 tACGGTATTGTTCCGGTGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATc                                                                  <  1:936485/100‑1 (MQ=255)
                                  aCGGTATCGATCCGGCGGATGTATAACGCCAAAACTTGCTATGCAGATATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATcc                                                                 <  2:2595982/100‑1 (MQ=255)
                                  aCGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCTGTTCATTCTCCTTATAAATcc                                                                 <  2:1529814/100‑1 (MQ=255)
                                   cGGTATCTATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCTATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                <  2:2095337/100‑1 (MQ=255)
                                   cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATCAATccc                                                                >  2:458025/1‑100 (MQ=255)
                                   cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                <  2:3233890/100‑1 (MQ=255)
                                   cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                >  1:61057/1‑100 (MQ=255)
                                   cGGTATCATTCCGGCATATGTAGAACACCGAATCTTGTGGTTCATAAACTGGCACAGTGCTCAATTTTCTTCATAGCCGCTCATTCTCCTTATAAATccc                                                                <  2:1835250/100‑1 (MQ=255)
                                      tATCGATCCGGTGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCa                                                              <  1:824827/99‑1 (MQ=255)
                                      tATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATcccc                                                               <  2:1285169/98‑1 (MQ=255)
                                         cGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCa                                                          >  1:717833/1‑100 (MQ=255)
                                          gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCaa                                                         >  1:2241926/1‑100 (MQ=255)
                                               ggcggATGTATAACACCAAAACTTACTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTAt                                                    >  2:3364871/1‑100 (MQ=255)
                                                 cggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATcc                                                  >  1:1477923/1‑100 (MQ=255)
                                                 cggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTAATTCTCCTTATAAATCCCCATCCAATTTATcc                                                  >  1:161394/1‑100 (MQ=255)
                                                   gATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAGTTTATccc                                                 <  2:1584348/99‑1 (MQ=255)
                                                     tGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTc                                              >  1:657806/1‑100 (MQ=255)
                                                      gTATAACACCAAATCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                             <  1:1479371/100‑1 (MQ=255)
                                                      gTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCGTTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                             <  2:1686398/100‑1 (MQ=255)
                                                      gTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCACCATCCAATTTATCCCTTCa                                             >  1:3662517/1‑100 (MQ=255)
                                                      gTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGCGCGCAATTTTCTGCATAGCTGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                             <  2:2124055/100‑1 (MQ=255)
                                                       tataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCat                                            >  1:404481/1‑100 (MQ=255)
                                                       tataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCat                                            >  1:2784823/1‑100 (MQ=255)
                                                       tataACACCAAAACTTGCTATGCAGAAATTTGCACAGAGCTCAATTTTCTGCATAGCCGCTCATTATCCTTATCAATCCCCATCCAATCTCTCCCttta                                             >  2:2400748/1‑97 (MQ=255)
                                                        ataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCata                                           >  2:948944/1‑100 (MQ=255)
                                                         taACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCACCATCCAATTTATCCCTTCatat                                          >  1:929811/1‑100 (MQ=255)
                                                          aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATAtt                                         <  2:962825/100‑1 (MQ=255)
                                                          aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATAtt                                         >  2:4011/1‑100 (MQ=255)
                                                           acacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTc                                        >  2:1402782/1‑100 (MQ=255)
                                                           acacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTc                                        <  1:1109356/100‑1 (MQ=255)
                                                           acacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTc                                        <  2:1319358/100‑1 (MQ=255)
                                                            cacCAAAACTTTCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAACTCTCCATCCAGTTTATCCCTTCATATTCa                                       >  2:3627584/1‑100 (MQ=255)
                                                            cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                       >  1:1242969/1‑100 (MQ=255)
                                                             acCGAAACTTGCTATGCAGAAATGTGCACAGTAAGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAAAACACATACAATTTATGCCTTAATATTCaa                                      >  2:3439074/1‑100 (MQ=255)
                                                                aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTa                                   <  1:1041360/100‑1 (MQ=255)
                                                                aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTa                                   >  1:45608/1‑100 (MQ=255)
                                                                 aaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAg                                  <  1:3677118/100‑1 (MQ=255)
                                                                   cgatcttATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                <  2:594005/94‑1 (MQ=255)
                                                                   aCTTGCTATGCAGATATTTGCACAGCGCGCACTTTTCTGCCTAGCTGCTCATTCTCCTTATTATTCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                <  2:317561/100‑1 (MQ=255)
                                                                   aCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                <  2:900839/100‑1 (MQ=255)
                                                                      ggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTAACACTTCATATTCAATTAGTTaaa                             >  1:2705543/2‑100 (MQ=21)
                                                                         tATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATaa                          >  1:2299203/1‑100 (MQ=25)
                                                                         tATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCAGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATaa                          >  2:1298180/1‑100 (MQ=25)
                                                                           ttcAGAAAATTTCGCACTGTACAAATTTCTAAATAGCCGCACATTCTCCTTAAAAATACCCATCCAATTTATCCCTTCATTTTCAATTATTAAAATaa                          >  2:3294734/3‑98 (MQ=255)
                                                                           tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                        >  2:1848760/1‑100 (MQ=255)
                                                                           tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                        >  1:2918679/1‑100 (MQ=255)
                                                                           tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                        >  2:15416/1‑100 (MQ=255)
                                                                            gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                       >  1:716742/1‑100 (MQ=37)
                                                                            gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                       >  1:1410585/1‑100 (MQ=25)
                                                                             cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                      <  2:2709819/100‑1 (MQ=255)
                                                                             cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                      >  2:2193415/1‑100 (MQ=37)
                                                                             cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                      >  1:2864081/1‑100 (MQ=37)
                                                                             cAGAAAATTGCGCACTGTGCAAATTTCTACATAGCCGCTCATTCTCCTTATAAATACACATCCAATTTATCCCTTCATCTTCAATCAGTTAAATAACTaa                      >  2:2670010/1‑100 (MQ=25)
                                                                              aGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaaa                     >  1:1161930/1‑100 (MQ=255)
                                                                               gAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAAt                    >  2:84850/1‑100 (MQ=255)
                                                                                      gCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaataa             >  2:876245/1‑100 (MQ=255)
                                                                                       cccactGCGCAATTTTCTGCATAGCCACTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAATTATATAACTAAATCCactaat            >  2:473116/6‑100 (MQ=255)
                                                                                        acaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtc           <  1:3047257/100‑1 (MQ=255)
                                                                                         caGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtct          >  2:1995201/1‑100 (MQ=255)
                                                                                             gtgcAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAtt      >  1:1166849/3‑100 (MQ=255)
                                                                                             gcgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAt       >  1:1712840/1‑99 (MQ=255)
                                                                                             gcgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTAAATTAGTAAAATAACTAAATCCAATAATCTCAtt      >  1:1334583/1‑100 (MQ=255)
                                                                                              cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTc     >  1:2941913/1‑100 (MQ=255)
                                                                                               gcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTAAAATAACTAAATCCAATAATCTCATTCt    >  1:1423996/1‑100 (MQ=255)
                                                                                               gcAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCt    <  1:2880790/100‑1 (MQ=255)
                                                                                                 aaTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTgg  <  1:2225390/100‑1 (MQ=255)
                                                                                                 aaTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTgg  <  1:2694258/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
AGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGG  >  NC_000913/2323192‑2323388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: