BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,380,380	T→C	26.6%	E88E (GAA→GAG)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,380,380	0	T	C	26.6%	188.4 / 76.8	129	E88E (GAA→GAG)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand): ref base T (42/51); new base C (9/25); total (51/78)
Fisher's exact test for biased strand distribution p-value = 6.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

CTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGC  >  NC_000913/2380283‑2380479

ntttACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTc                                                                                                   >  2:1436238/2‑100 (MQ=255)

cTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTc                                                                                                   >  2:716333/1‑100 (MQ=255)

  ttACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCg                                                                                                 <  1:546009/100‑1 (MQ=255)

    aCCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCggg                                                                                               >  2:3629666/1‑100 (MQ=255)

     ccGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGt                                                                                              >  1:2155861/1‑100 (MQ=255)

      cGCCACAGTGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGTCCGGCGATGGGTCAAAAGCATTCAGCCCCTAAATGCGTAAGTCGGCGTGTTCCGGGTg                                                                                             <  2:1263469/100‑1 (MQ=255)

      cGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAATGCATTCAGCCCTCAAATGCGTAAGTCGGCGTGTTCCGGGTg                                                                                             <  2:1262467/100‑1 (MQ=255)

       gCCACAGCGTCGCCATTCGAGGCGTGGTAAAAGTAAATTTCCCTGCGACGGGTCAAACGCATTCAGCCCCCAGATGCGTAAGTCGGCGTGTTCCGGGTGc                                                                                            <  1:1230002/100‑1 (MQ=255)

       gCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGc                                                                                            >  2:1388232/1‑100 (MQ=255)

       gCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGCCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTCTTCCGGGtac                                                                                            >  2:382797/1‑98 (MQ=255)

        ccACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCt                                                                                           >  2:1680213/1‑100 (MQ=255)

          acaGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTgg                                                                                         <  1:3226273/100‑1 (MQ=255)

              cGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaa                                                                                     >  1:329581/1‑100 (MQ=255)

              cGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCgaa                                                                                     <  2:1239452/100‑1 (MQ=255)

               gTCGCTATTTCTGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaag                                                                                    <  1:1752983/100‑1 (MQ=255)

               gTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaag                                                                                    <  1:805013/100‑1 (MQ=255)

               gTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaag                                                                                    >  2:2097505/1‑100 (MQ=255)

               gTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCgaag                                                                                    <  2:1065541/100‑1 (MQ=255)

                tCGTCATTCCAGTCGTGTTAAAAGTTATTTTCCTTGCGATGGGTCAAAAGCATGCAGCCCACAAAAGCGGAAGTCGGCGTGTTCCGGGTGCTGGCgaag                                                                                    <  1:577209/99‑1 (MQ=255)

                tCGCAATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCgaag                                                                                    <  2:3498861/99‑1 (MQ=255)

                 ctccATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCgaag                                                                                    <  2:69673/96‑1 (MQ=255)

                 cGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaagaa                                                                                  >  1:14671/1‑100 (MQ=255)

                 cGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaagaa                                                                                  >  1:3745609/1‑100 (MQ=255)

                 cGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCATAAATCGGCGTGCTCCAGGTGCTGGCgaagaa                                                                                  >  1:1750858/1‑100 (MQ=255)

                   ccATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGaaaa                                                                                <  1:3036749/100‑1 (MQ=255)

                   ccATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGaaaa                                                                                >  2:488789/1‑100 (MQ=255)

                    cATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGaaaa                                                                                <  2:3764338/99‑1 (MQ=255)

                     aTTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACg                                                                              <  1:3137456/100‑1 (MQ=255)

                      ttCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGt                                                                             >  2:3925470/1‑100 (MQ=255)

                       tCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGtt                                                                            <  1:921028/100‑1 (MQ=255)

                         cAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTtg                                                                           <  2:807540/99‑1 (MQ=255)

                          aGGCGGGGTAAAAGTAAATTGCCTTGTGATGGTTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTtgtg                                                                         <  1:675284/100‑1 (MQ=255)

                          aGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTtgtg                                                                         >  1:494838/1‑100 (MQ=255)

                          aGGCGAGTTAAAAGTAAATTGCCCTGCGACCGGTCAAGAGCATTCGGCCCACATTTGCGTAAGTCGGCGTGTTCCTGGTGCTGGCGAAGTAAGCGTtgtg                                                                         <  2:370588/100‑1 (MQ=255)

                             cGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGTGTGCTGGCGAAGAAATCGTTGTGcc                                                                       <  2:1029075/99‑1 (MQ=255)

                             cGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCt                                                                      >  2:475445/1‑100 (MQ=255)

                               gggTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTgg                                                                    <  2:399312/100‑1 (MQ=255)

                                ggTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGt                                                                   >  2:3093093/1‑100 (MQ=255)

                                  tAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTcc                                                                 >  2:3018057/1‑100 (MQ=255)

                                  tAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGTTGCTGACGAAGAAAACGTTGTGCCTGGTcc                                                                 >  1:941634/1‑100 (MQ=255)

                                   aaaaGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCa                                                                >  2:1346172/1‑100 (MQ=255)

                                     aaGTAAATTGCCTTTCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAac                                                              <  1:3715597/100‑1 (MQ=255)

                                      aGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGAGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAac                                                              >  1:3487269/1‑99 (MQ=255)

                                       gTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAacga                                                            >  1:2487542/1‑100 (MQ=255)

                                         aaaTTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAacgacg                                                          <  1:2880588/100‑1 (MQ=255)

                                         aaaTTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAacgacg                                                          <  1:1384223/100‑1 (MQ=255)

                                          aaTTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGt                                                         <  2:198068/100‑1 (MQ=255)

                                               cccTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAAc                                                    >  1:835382/1‑100 (MQ=255)

                                               cccTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACAACGTAAAAc                                                    <  2:1637776/100‑1 (MQ=255)

                                                ccTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACg                                                   <  1:341550/100‑1 (MQ=255)

                                                 cTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCGGGCGAAGAAAACGTTTTGCCTGGTCCAACGACGTAAAACgg                                                  <  2:2136625/100‑1 (MQ=255)

                                                  tGCGACGGTTCAAAAGCATTCAGCCCCCAAATGCGTCAGTCGTCGTGTTCCGGGTGCTGGCCAAGAAAACTTTGTGCCTGGTCCAACGACGTAAAACggg                                                 <  2:917498/100‑1 (MQ=255)

                                                  tGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACggg                                                 >  1:2302446/1‑100 (MQ=255)

                                                   gCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGt                                                <  2:2159656/100‑1 (MQ=255)

                                                    cGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGTCTGGTCCAACGACGTAAAACGGGTa                                               <  2:3470872/100‑1 (MQ=255)

                                                      aCGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAAt                                             >  1:530666/1‑100 (MQ=255)

                                                       cGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATc                                            <  1:3827171/100‑1 (MQ=255)

                                                       cGGGTCAAAAGCATTCAGCCCCAAAATGCGTAAGTCGGCGTGTTCCGAGTGCTGACGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATc                                            >  2:796436/1‑100 (MQ=255)

                                                        gggTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATcg                                           <  2:2178311/100‑1 (MQ=255)

                                                         ggTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATcgc                                          >  2:3202653/1‑100 (MQ=255)

                                                         ggTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATcgc                                          >  2:2244044/1‑100 (MQ=255)

                                                          gTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATcgcg                                         <  1:1221616/100‑1 (MQ=255)

                                                           tCAAAAGCATTCAGCTCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCATGGTCCAACGACGTAAAACGGGTAATCgcgc                                        >  2:1365903/1‑100 (MQ=255)

                                                             aaaaGCATTCAGCCCCCAAATTCGTAAGTCGGCGTGTTCTGTGTGCTGGCGAAGAAAACGTAGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGccc                                      <  1:1807233/100‑1 (MQ=255)

                                                             aaaaGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCTAACGACGTAAAACGGGTAATCGCGccc                                      <  2:28635/100‑1 (MQ=255)

                                                              aaaGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCa                                     <  1:906299/100‑1 (MQ=255)

                                                                 gCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAg                                    <  2:722714/98‑1 (MQ=255)

                                                                  cATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgac                                 >  1:2716409/1‑100 (MQ=255)

                                                                   aTTCAGCCCCCAAATTCGTAAGGCGGCCTGTTCCGGGTGCTGGCGAAGAAAACTTTTTGCTTGGCCCAACCACCTAAAACGGTTAATCGCGCTCAg                                    <  2:1501660/96‑1 (MQ=255)

                                                                    ttCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgacga                               >  1:2985029/1‑100 (MQ=255)

                                                                    ttCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCACAGgacga                               >  1:238980/1‑100 (MQ=255)

                                                                     tCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgacgac                              <  1:2214689/100‑1 (MQ=255)

                                                                     tCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgacgac                              >  1:2047512/1‑100 (MQ=255)

                                                                     tCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgacaga                              >  2:3704466/1‑97 (MQ=255)

                                                                     tCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgacgac                              <  2:3248539/100‑1 (MQ=255)

                                                                     tCAGCCCCCAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGgacgac                              <  1:3389759/100‑1 (MQ=255)

                                                                      cAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACa                             <  1:1477475/100‑1 (MQ=255)

                                                                       aGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAg                            <  1:3156935/100‑1 (MQ=255)

                                                                         cccccAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGTGTAATCGCGCCCAGGACGACAGct                          <  2:326662/100‑1 (MQ=255)

                                                                         cccccAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGct                          >  1:2584326/1‑100 (MQ=255)

                                                                         cccccAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGct                          >  1:3861174/1‑100 (MQ=255)

                                                                         cccccAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGcn                          <  2:1578346/100‑2 (MQ=255)

                                                                           cccAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATAGCGCCCAGGACGACAGcttc                        >  2:3320271/1‑100 (MQ=255)

                                                                           cccAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACAGGTAATCGCGCCCAGGACGACAGcttc                        >  1:2081817/1‑100 (MQ=255)

                                                                           cccAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGctt                         >  2:3134350/1‑99 (MQ=255)

                                                                            ccAAATGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGcttct                       <  1:851867/100‑1 (MQ=255)

                                                                              aaaTGCGTAAGTCGGCGTGGTCCGGGTGCTGGCGAAGAAAACTCTGTGCCTGTTCTAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCttcttc                     <  2:3335147/100‑1 (MQ=255)

                                                                                aTGCGTAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTtcat                   <  2:2445368/100‑1 (MQ=255)

                                                                                 tGCGTAAGTCGGCGTGCTCCGGGTTTTGTTGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTtcatc                  <  1:1497931/100‑1 (MQ=255)

                                                                                    gTAAGTCGGCGTGGTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACTTAAAACTGGTAATCGCGCCCAGGACGACAGCTTCTtcatca                 <  2:746177/98‑1 (MQ=255)

                                                                                     tAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCa              <  2:3560316/100‑1 (MQ=255)

                                                                                     tAAGTCGGCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCa              <  2:330147/100‑1 (MQ=255)

                                                                                        gTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTa           <  2:846279/100‑1 (MQ=255)

                                                                                           ggCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACgt        <  2:2631298/100‑1 (MQ=255)

                                                                                           ggCGTGCTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACgt        >  2:1395127/1‑100 (MQ=255)

                                                                                            gCGTGCTCCGTGTGCTGGCGAATAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACgt        <  2:941623/99‑1 (MQ=255)

                                                                                             cGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACgttg      <  1:1388247/100‑1 (MQ=255)

                                                                                                gtTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGttgttg   <  2:1662853/100‑1 (MQ=255)

                                                                                                gtTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGttgttg   >  2:1035837/1‑100 (MQ=255)

                                                                                                 ttCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGc  <  2:447210/100‑1 (MQ=255)

CTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGC  >  NC_000913/2380283‑2380479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`