Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,391,417 C→G 17.9% W30S (TGG→TCG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,391,4170CG17.9% 311.7 / 55.0 133W30S (TGG→TCG) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (62/46);  new base G (10/14);  total (73/60)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 136 total aligned reads displayed.

ACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACG  >  NC_000913/2391318‑2391516
                                                                                                   |                                                                                                   
aCGTCCATAGCGCCCGCCTGGCCAACACACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGATAGCGTAGCGTTGTTGAAATGATTGCGTCGcc                                                                                                     <  2:348304/100‑1 (MQ=255)
aCGTCCATAGCGCCCGCCTGGCCAACAAACCTGAGCGAAACCAGCGCCACGTTAAGACCAATAAACGAAAGCGTAGCGTTGAGGAAATGATTGCGTCGcc                                                                                                     >  1:65100/1‑100 (MQ=255)
  gcccACAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCTCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCAc                                                                                                   <  2:1300954/98‑1 (MQ=255)
     cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                                <  1:1376120/100‑1 (MQ=255)
       tAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTAGCCACGCaa                                                                                               >  2:1705064/1‑99 (MQ=255)
        aGCGCCCGCCTTGCCAACAAACCCAAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGTAAATGATTGCGTGGCGACGCAAtc                                                                                             <  2:2709889/100‑2 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtca                                                                                            <  2:532231/100‑3 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                            >  1:2168308/1‑100 (MQ=255)
          cgcccgccTGTCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGTGTTGAGGAAATGATTGCGTGGCGACGCAAtcat                                                                                           <  2:2575001/100‑4 (MQ=255)
            cccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCTAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgaga                                                                                         <  2:552185/100‑1 (MQ=255)
            cccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcattt                                                                                         <  2:2906107/100‑6 (MQ=255)
            cccgccTGGCAAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgaga                                                                                         >  1:3749689/1‑100 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  1:3718009/100‑8 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  2:1024702/100‑8 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGc                                                                                       <  2:3839937/100‑1 (MQ=255)
               gccTGGCCAACAAACCAGAGTGAAACCAGCGCCGTGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCa                                                                                      <  1:2234842/100‑1 (MQ=255)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcct                                                                                      <  1:1206330/100‑9 (MQ=38)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGGAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCa                                                                                      <  2:2137928/100‑1 (MQ=255)
                 cTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGAGTAGCGTTGGGGAAATGATTGGGTCGCCACGCAATGGAGAGCATc                                                                                    >  2:600880/1‑100 (MQ=255)
                  tGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                   <  2:1101546/100‑1 (MQ=255)
                   ggCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATcac                                                                                  <  2:774217/100‑1 (MQ=255)
                   ggCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATcac                                                                                  >  1:420040/1‑100 (MQ=255)
                     ccAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACaa                                                                                >  1:1715233/1‑100 (MQ=255)
                     ccAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGCCGAGGAAATGATACCGTCGCCACGCAATCgagcgtctcagca                                                                                >  2:3282316/1‑90 (MQ=255)
                      caacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAac                                                                               >  2:1198138/1‑100 (MQ=255)
                       aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacc                                                                              <  1:2890876/100‑1 (MQ=255)
                       aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacc                                                                              <  1:776321/100‑1 (MQ=255)
                        acaaACCAGAGCGAAACCAGTGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAacca                                                                             <  1:1439470/100‑1 (MQ=255)
                            aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTACGTCACCACGCAATGGAGAACATCACAaccaccac                                                                         >  1:1481342/1‑100 (MQ=255)
                             ccAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAAGAAATGATTGCGACGCCACGCAATGGAGAGCATCACAaccaccacc                                                                        >  1:133828/1‑100 (MQ=255)
                              cAGAGCGAAACCAGCGCCGTGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                       <  1:954939/100‑1 (MQ=255)
                              cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                       <  1:3151543/100‑1 (MQ=255)
                              cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCg                                                                       >  1:679351/1‑100 (MQ=255)
                               agagCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGt                                                                      <  2:848507/100‑1 (MQ=255)
                                 agCGAAACCAGCGCCGCGTTAAGCCCAATAACCTAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGt                                                                      <  1:600887/98‑1 (MQ=255)
                                  gCGAAACCAGGGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGGGGAAATGATGGCGTCGCCACGCAAAGGAGAGCATCACAaccagcac                                                                         >  2:3436274/1‑94 (MQ=255)
                                  gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGCGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCaa                                                                   >  1:1787950/1‑100 (MQ=255)
                                  gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCaa                                                                   <  1:1697617/100‑1 (MQ=255)
                                  gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACCCAAAGGAGAGCATCACAACCACCACCGTCaa                                                                   >  1:1930268/1‑100 (MQ=255)
                                  gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGATGAGGAAATGATTGCGCCGCCACGCAATGGAGAGCATCACAACCACCACCGTCaa                                                                   >  2:2298939/1‑100 (MQ=255)
                                   cGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAg                                                                  <  2:276600/100‑1 (MQ=255)
                                   cGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAg                                                                  >  1:1104658/1‑100 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAg                                                                  <  2:2891423/99‑1 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                 >  1:1399403/1‑100 (MQ=255)
                                       aCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcga                                                              >  2:1507775/1‑100 (MQ=255)
                                       aCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTACGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcga                                                              >  1:503191/1‑100 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                             <  2:2704942/100‑1 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcga                                                              >  2:1010285/1‑99 (MQ=255)
                                             gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                        >  2:3581853/1‑100 (MQ=255)
                                               cgcgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGc                                                      <  2:3484943/100‑1 (MQ=255)
                                                gcgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCa                                                     <  1:2067374/100‑1 (MQ=255)
                                                  gTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACAGTCAAGCCGACGATCAGCaa                                                    >  1:1921649/1‑99 (MQ=255)
                                                       gCCCAATAACCGAGAGCGTAGCGTTGTGGAAATGATTGCGTCGCCACGCAATGGCGAGCATCAGAATCACCACCGTGAAGCCGACAATCAGCAACGGTAg                                              >  2:1745470/1‑100 (MQ=255)
                                                       gCCCAATAACCGAGAGCGTAGCGTTGAGTAAATGATTGCGTCGCCACGCAATTGAGAGCATCACAACCACCACCGTCAATCCGACGATCAGCAACGGTa                                               <  2:3749650/99‑1 (MQ=255)
                                                       gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAg                                              >  1:3349598/1‑100 (MQ=255)
                                                       gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAg                                              >  1:1559055/1‑100 (MQ=255)
                                                        gatcttAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  2:2368252/95‑1 (MQ=255)
                                                        cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  1:1045195/100‑1 (MQ=255)
                                                        cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             >  2:3093577/1‑100 (MQ=255)
                                                             tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAgatc                                        >  1:2368274/1‑97 (MQ=255)
                                                             tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                        >  2:297943/1‑100 (MQ=255)
                                                             tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                        <  2:110538/100‑1 (MQ=255)
                                                                ccGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGTGCATCACAACCACCAACGACAAGCCGACGATCAGCAACGGTAGCAGTGCGAt                                     >  2:1737161/1‑100 (MQ=255)
                                                                ccGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                      >  2:1062614/1‑99 (MQ=255)
                                                                 cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATc                                    >  1:277490/1‑100 (MQ=255)
                                                                  gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCATGTAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCa                                   <  1:1245889/100‑1 (MQ=255)
                                                                  gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCa                                   <  2:2071848/100‑1 (MQ=255)
                                                                  gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCa                                   >  2:2345766/1‑100 (MQ=255)
                                                                    gagCGTAGCGTTGAGGAAATGATTGCGTTGCCACGCAATGGAGAGTATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAgg                                 <  1:15548/100‑1 (MQ=255)
                                                                     agcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGt                                <  2:918/100‑1 (MQ=255)
                                                                      gcgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                               <  2:519355/100‑1 (MQ=255)
                                                                       cgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGttt                              <  2:252567/100‑1 (MQ=255)
                                                                            cggtGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTgg                         >  2:3275512/4‑100 (MQ=255)
                                                                             gtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                        <  2:3351686/100‑1 (MQ=255)
                                                                               tGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAg                       >  1:1944879/1‑99 (MQ=255)
                                                                                gAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGtt                     >  1:3018307/1‑100 (MQ=255)
                                                                                gAGGAAATGATTGCGTCGCAACGCAATGGAGAGCATAACAACCACCACAGTCAAGCCGACGAGCAGCAACAGTAGCAGTGCGATCAGGGTTTGTGGAGtg                     >  2:2274233/1‑99 (MQ=255)
                                                                                 aGGAAATGATTGCGTCGCCACGCAATGGAGAGTATTACAATCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTa                    <  1:1259903/100‑1 (MQ=255)
                                                                                  ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAt                   >  1:1346001/1‑100 (MQ=255)
                                                                                   gAAATGATTGCTTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                  <  1:381703/100‑1 (MQ=255)
                                                                                    tctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAt                   >  1:2931735/6‑98 (MQ=255)
                                                                                    tctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 >  2:3616551/6‑100 (MQ=255)
                                                                                    aaaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                  >  2:2140013/1‑99 (MQ=255)
                                                                                    aaaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 >  2:2043535/1‑100 (MQ=255)
                                                                                     ctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGt                >  2:773017/5‑100 (MQ=255)
                                                                                      aTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTc               <  2:124593/100‑1 (MQ=255)
                                                                                       tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  1:2101793/1‑100 (MQ=255)
                                                                                       cccttGCGTGGCGACGCAATGGAGAGCATCAAAACCACCCCCGTCAAGCGGACGATCCGCAACGCCAGCAGTACGATCAAGTTTTGAGGAGTTATTGt                >  2:105987/4‑98 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  1:1061599/3‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGAAGTGCGATCAGGTTTTGTGGAGTTATTGtga              >  2:3708807/3‑98 (MQ=255)
                                                                                        caTTGCGTGGCGACGCAATGGCGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCAt             >  1:2967805/2‑100 (MQ=255)
                                                                                           ctcGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGc          <  2:2157228/98‑1 (MQ=255)
                                                                                            gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCTATCAGTTTTTGTGGAGTTATTGTCATgga          >  1:828059/1‑98 (MQ=255)
                                                                                            gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCg         <  2:3028136/100‑1 (MQ=255)
                                                                                            gCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAAAAGTGCGATCAGGTTTTGAGGAGTTATTGTCATGGCg         >  1:258918/1‑100 (MQ=255)
                                                                                               tCGCCACGCAATGGAGAGGATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAtt      <  2:1025268/100‑1 (MQ=255)
                                                                                                cGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCCGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAttt     >  2:1444788/1‑100 (MQ=255)
                                                                                                cGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAttt     >  2:1444840/1‑100 (MQ=255)
                                                                                                cGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAttt     >  2:1273545/1‑100 (MQ=255)
                                                                                                   cACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg  >  1:1652743/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACG  >  NC_000913/2391318‑2391516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: