Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,414,689 C→G 15.3% intergenic (+17/‑58) ackA → / → pta acetate kinase A and propionate kinase 2/phosphate acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,414,6890CG15.3% 394.7 / 58.2 159intergenic (+17/‑58)ackA/ptaacetate kinase A and propionate kinase 2/phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (67/66);  new base G (18/6);  total (85/74)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01

Only 100 of 174 total aligned reads displayed.

AAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTC  >  NC_000913/2414590‑2414788
                                                                                                   |                                                                                                   
aagaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctc                                                                                                     >  1:3566083/1‑100 (MQ=255)
aagaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctc                                                                                                     <  1:3446842/100‑1 (MQ=255)
  gaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcag                                                                                                   <  1:2137269/100‑1 (MQ=255)
  gaagGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcag                                                                                                   <  2:1968981/100‑1 (MQ=255)
    agGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCcagctcagct                                                                                                 >  1:2197171/1‑100 (MQ=255)
     ggTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTg                                                                                                <  1:1833144/100‑1 (MQ=255)
       tACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGc                                                                                              >  1:3672954/1‑100 (MQ=255)
       tACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGc                                                                                              >  2:2996329/1‑100 (MQ=255)
         cccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCgg                                                                                            <  2:479844/100‑1 (MQ=255)
          ccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGt                                                                                           >  1:1067480/1‑100 (MQ=255)
           cGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTGAGCTGGCGGTg                                                                                          <  1:1435895/100‑1 (MQ=255)
           cGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTGAGCTGGCGGTg                                                                                          <  1:2293968/100‑1 (MQ=255)
            gTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGTGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTGAGCTGGCGGTgt                                                                                         <  1:3028290/100‑2 (MQ=255)
            gTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACGGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGc                                                                                         <  1:1396766/100‑1 (MQ=255)
             tcatGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTGAGCTGGCGGTgtg                                                                                        <  1:1911660/97‑3 (MQ=255)
             tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCt                                                                                        >  2:3876283/1‑100 (MQ=255)
              ccTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTg                                                                                       <  1:2439411/100‑1 (MQ=255)
               cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGt                                                                                      >  2:1665262/1‑100 (MQ=255)
                tGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGtt                                                                                     <  1:2814631/100‑1 (MQ=255)
                tGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGtt                                                                                     <  1:711809/100‑1 (MQ=255)
                 gCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGttt                                                                                    <  1:815062/100‑1 (MQ=255)
                   ggtggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGttt                                                                                    <  2:930826/98‑1 (MQ=255)
                   ggtggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTg                                                                                  <  1:2587636/100‑1 (MQ=255)
                    gtggtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGt                                                                                 <  2:3115344/100‑1 (MQ=255)
                    gtgggTATCACAACCTAGGAAGAACTGTTTAACGCGCAAGACTCGAGCCGCCTGACTGCCTGATTTCACACAGCCAGCTCAGCTGGCGATg                                                                                          <  2:814724/91‑1 (MQ=255)
                     tggtTATTCCAACTAACGAAGAATTTGTTATCGTGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTGAGCTGGCGGTgtggaatcagg                                                                                <  1:1905056/100‑11 (MQ=25)
                          aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCg                                                                           <  2:830766/100‑1 (MQ=255)
                          aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCg                                                                           >  1:3896022/1‑100 (MQ=255)
                           tCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGc                                                                          >  2:223753/1‑100 (MQ=255)
                           tCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCAAGCTCAGATGGCGGTGCTGTTTTGTAACCCGc                                                                          >  1:1285882/1‑100 (MQ=255)
                            cccacccaACGAAGAACGGGTTATCGCGCAAGTCGCGAGCCGCCTGACTGCCTGATTTCACCCCGCCAGCTCAGCTGGCGGTGCTGTTATGTAACCCg                                                                           <  2:89799/98‑1 (MQ=255)
                            cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGTCTGATTTCACACCGCCATCTCAGCTGGCGGTGCTGTTTTGTAACCCg                                                                           <  2:2868489/98‑1 (MQ=255)
                             ccaaccaaCGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCa                                                                        >  2:2230681/1‑100 (MQ=255)
                               aaccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCaaa                                                                      <  2:2589993/100‑1 (MQ=255)
                                  caacGAAGAACTGGGTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCTGCTGGCGGTGCTGTTTTGTAACCCGCCAAAtn                                                                    <  2:2319618/99‑2 (MQ=255)
                                   aacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcgg                                                                  <  2:3555930/100‑1 (MQ=255)
                                   aacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAACcg                                                                   >  2:2896892/1‑99 (MQ=255)
                                    ccGAAGAACTGGTCATCGCGCAAGACGCGAGCCTCGTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggn                                                                 <  2:1796003/99‑2 (MQ=255)
                                    acGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggc                                                                 <  1:3137325/100‑1 (MQ=255)
                                     cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggcg                                                                >  1:2962269/1‑100 (MQ=255)
                                       aagaaCTGGTTATCGCGCAAGACGCGAGTCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGt                                                              <  1:2962088/100‑1 (MQ=255)
                                        agaaCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGATCAGCTGTCGGTGCTGTTTTGTAACCCGCCAAATAGGCGGTa                                                             >  1:369242/1‑100 (MQ=255)
                                           aCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACg                                                          <  1:1009289/100‑1 (MQ=255)
                                           aCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACg                                                          >  1:268718/1‑100 (MQ=255)
                                            cTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTAGCGGCGCTGTTTTGTAACCCGACAAATCGGCGGTAACGa                                                         >  1:96407/1‑100 (MQ=255)
                                                 tATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAAGGAAagag                                                    >  2:2097912/1‑100 (MQ=255)
                                                  aTCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAgg                                                   >  2:153552/1‑100 (MQ=255)
                                                   tctagcAAGACGCGAGCCGCCTGACTGCCTGATTTCACAACGCCAGCTTAGATGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGa                                                  <  2:1463439/96‑1 (MQ=255)
                                                   tCGCGCAAGACGCGAGCCGCCTGTTTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAgg                                                   <  1:1580451/99‑1 (MQ=255)
                                                   tCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAgg                                                   <  1:2624570/99‑1 (MQ=255)
                                                     gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTATCCCGCCAAATCGGAGGTAAAGAAAGAGGATa                                                <  2:1577219/100‑1 (MQ=255)
                                                     gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATa                                                >  2:2780801/1‑100 (MQ=255)
                                                     gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATa                                                >  2:1300320/1‑100 (MQ=255)
                                                     gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATa                                                >  2:1589227/1‑100 (MQ=255)
                                                      cgcAAGACGCGAGCCGCCTGACTGCCTGATCTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                 <  2:681212/98‑1 (MQ=255)
                                                        cAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATaaa                                              >  1:665896/1‑99 (MQ=255)
                                                         aaGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAAcc                                            <  1:1013402/100‑1 (MQ=255)
                                                         aaGACCCGATCCGCCTTACTGCCTTATTTCACACCTGCAGCTCAGCGGGCGGTGCTGTTTTGTAACCCTCCAAATCTGCTGTAACCAAAGAGGATaaccg                                            <  2:3802451/100‑4 (MQ=255)
                                                                 aGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                    <  2:2915572/100‑1 (MQ=255)
                                                                  gccgccTGACTGCCTGTTTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGt                                   <  2:2288437/100‑1 (MQ=255)
                                                                  cccgccTGCCTGCCTGATTTCACACCGCCAGCTCAGCTGGCGCTGCTGTTTTGTAACCCGCCACATCGGCGGTAACGAAAGAGGATAAAcc                                            >  2:1221688/2‑91 (MQ=255)
                                                                     gccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtatt                                <  1:2893226/100‑1 (MQ=255)
                                                                         gACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatta                            <  2:3087168/100‑1 (MQ=255)
                                                                          aCTGCCTGATTTTACACCGCTAGCTTTGCTGGCTGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatta                            <  1:1550858/99‑1 (MQ=255)
                                                                            ttccTGATTTCACACCGCCAACTCAGCTGGCGGTGCTGTTTTGTAACCCACCACATCGGCGTTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGc                         >  2:1203507/3‑100 (MQ=255)
                                                                            tGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGc                         <  1:3421480/100‑1 (MQ=255)
                                                                             gCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTTTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATTCt                        >  2:1751649/1‑100 (MQ=255)
                                                                               cTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTg                       <  2:2716703/99‑1 (MQ=255)
                                                                                caaaacagcacCGCCAGCTGAGCTGGCGGTGTTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACAGTGTCCCGTATTATTATGCTGAt                     >  1:3580946/9‑100 (MQ=37)
                                                                                   tttCGCACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAGCCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGGCCCGTATTATTATGCTGATcc                   <  2:15568/99‑1 (MQ=255)
                                                                                     tCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTa                >  2:3687943/1‑100 (MQ=255)
                                                                                      cacaCCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAc               >  2:2955924/1‑100 (MQ=255)
                                                                                      cacaCCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAc               >  1:2494348/1‑100 (MQ=255)
                                                                                       gcacCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTCACGCAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAcc              >  2:1720381/2‑100 (MQ=255)
                                                                                       gcacCGCCAGCTGAGCTGGAGGTGCTGTTTTGTAACCCGCCAAATCAGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAcc              >  2:2470551/2‑100 (MQ=255)
                                                                                       acacCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAcc              <  1:3478443/100‑1 (MQ=255)
                                                                                       acacCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAcc              <  2:2836968/100‑1 (MQ=255)
                                                                                       acacCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAcc              >  2:1517336/1‑100 (MQ=255)
                                                                                        catcGCCAGCTCAGCTGGCGGTGCTGTTTTGTCACTCGCCTAATCGGTTTTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCg             <  2:3643033/97‑1 (MQ=255)
                                                                                        cacCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCg             >  1:3180036/1‑100 (MQ=255)
                                                                                        cacCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCg             >  1:2714152/1‑100 (MQ=255)
                                                                                        cacCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCg             >  1:3799407/1‑100 (MQ=255)
                                                                                        cacCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCACCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATACCTACCg             >  2:2085723/1‑100 (MQ=255)
                                                                                        cacCGCCAGCTCAGCTGGCGGTGCTTTTTTGTAACCCGCCAAATAGGAGGTAAAGAAAGAGAAAAAACAGTGTAACGTATTATTATGCTGATcaatatag             >  1:1226137/1‑93 (MQ=255)
                                                                                         acggCCAGCTGACCTGGCGGAGCTGTTTTGTAACCCGCCAAAGCGGCGGTAACGAAAGACGATAAACCGTATCCCGTACTATTATGCTGATCACTAcc              >  2:1143965/4‑98 (MQ=255)
                                                                                         acCGCCAGCTGAGCTGGCGGTGTTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCgg            >  1:2327053/1‑100 (MQ=255)
                                                                                         acCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCgg            >  1:2065534/1‑100 (MQ=255)
                                                                                         acCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCACGTATTATTATGCTGATCCCTACCgg            >  1:1350359/1‑100 (MQ=255)
                                                                                         acCGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGCAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCgg            >  2:386426/1‑100 (MQ=255)
                                                                                         acCGCCAGCTGAGCTGGCGGTGCAGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTAGTATTATGCTGATCCCTACCgg            >  2:1957292/1‑100 (MQ=255)
                                                                                          ccGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGAATCCCGAATAATTATGCTGATCCCTACCGGa           >  1:437353/1‑100 (MQ=255)
                                                                                           cGCCAGCTGAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCAGATcggaagagcgt          >  2:1648747/1‑90 (MQ=255)
                                                                                            gCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGCAc         >  2:247420/1‑100 (MQ=255)
                                                                                            gCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAAc         <  1:2599000/100‑1 (MQ=255)
                                                                                             ccAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAAcc        <  1:2059020/100‑1 (MQ=255)
                                                                                                gctcagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCa       >  2:2739973/1‑98 (MQ=255)
                                                                                                  tcagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGt   >  2:1869381/1‑100 (MQ=255)
                                                                                                   cagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTc  >  1:1829505/1‑100 (MQ=255)
                                                                                                   cagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTc  >  1:1129543/1‑100 (MQ=255)
                                                                                                   cagctGGCGGTGCTGTTTTGTAACCCGACAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTc  >  2:3640274/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTC  >  NC_000913/2414590‑2414788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑