Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,228 C→A 15.3% intergenic (+23/+29) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2280CA15.3% 331.7 / 42.9 137intergenic (+23/+29)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (54/56);  new base A (16/5);  total (72/65)
Fisher's exact test for biased strand distribution p-value = 3.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-02

Only 100 of 143 total aligned reads displayed.

AAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAC  >  NC_000913/2519129‑2519327
                                                                                                   |                                                                                                   
aaaaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                     <  1:2827637/100‑1 (MQ=255)
aaaaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                     >  2:391304/1‑100 (MQ=255)
aaaaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                     <  1:571623/100‑1 (MQ=255)
 aaaaGTCGCGCAATTTTTTTCACGTGAATGAATTCGCAATTTCCTCGCCCGTCACGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtct                                                                                                    <  2:3254219/100‑1 (MQ=255)
     gTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCt                                                                                                 >  1:2924821/1‑99 (MQ=255)
      tCGCGCAATTTTTGTCACGTGAAGTAATTTGCAATTTCCTCGTCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                               <  1:58430/100‑1 (MQ=255)
      tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                               <  1:135125/100‑1 (MQ=255)
      tCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                               <  2:1089354/100‑1 (MQ=255)
      tCGCGCAATTTTTGTCACGTGAAGGAATTCGAAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                               <  1:2714595/100‑1 (MQ=255)
       cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCGGTCTCGATATTGAGGAAGCAGCATAAATAAAAAACGCCAGGATATTAGGTCCTga                                                                                               >  2:3931802/1‑98 (MQ=255)
       cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                               >  2:3660943/1‑99 (MQ=255)
        gcgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGTTATTATCTCCTGCCg                                                                                             <  2:1414804/100‑1 (MQ=255)
        gcgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCg                                                                                             >  1:1672816/1‑100 (MQ=255)
         cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGcc                                                                                              <  2:1398414/98‑1 (MQ=255)
         cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGt                                                                                            <  1:3532470/100‑1 (MQ=39)
          gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                           <  1:3717622/100‑1 (MQ=255)
          gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATAAAAAAAAAAAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                           <  2:2813860/100‑1 (MQ=255)
          gcAATTTTTGTCACATGAAGGAATTCGCACTTTCCTCCCCCGTCTCTATATTAACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTTCTg                                                                                                >  2:509162/1‑95 (MQ=255)
           cAATTTTGGGCACGGGAAGGAAGTTACAATTTCCAAGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                <  2:265645/94‑1 (MQ=255)
                   gTCACGTTAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                  <  1:1048137/100‑1 (MQ=255)
                   gTCACGTGAAGGAATTCGCAATTTCCTCTCCCGTCTCGATTTTTACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                  <  1:80651/100‑1 (MQ=255)
                     cACGTGAAGGAATTCGCAATTTGCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttttatttatg                                                                                <  2:2257620/100‑11 (MQ=25)
                     cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTTGATATTTACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttttatttatg                                                                                <  1:3605721/100‑11 (MQ=21)
                     cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                                <  1:3319408/100‑1 (MQ=255)
                     cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                                >  2:2263867/1‑100 (MQ=255)
                     cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTac                                                                                >  1:2353473/1‑100 (MQ=255)
                       cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTacac                                                                              >  1:1034520/1‑100 (MQ=255)
                         tGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                            >  1:41829/1‑100 (MQ=255)
                          gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCt                                                                           <  1:3437352/100‑1 (MQ=255)
                           aaGGAATTCGCAATTACATCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAATCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTacac                                                                              <  2:867434/96‑1 (MQ=255)
                               aaTTCGCAATTTCCTCGCCCGTCTCGATATTGACGCATCAGCATAAATAAACAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTa                                                                      >  1:2871637/1‑100 (MQ=255)
                                aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                     >  1:309134/1‑100 (MQ=255)
                                aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                     >  1:309702/1‑100 (MQ=255)
                                aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                     <  1:1485165/100‑1 (MQ=255)
                                aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                     >  1:3215408/1‑100 (MQ=255)
                                aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACAGCAAGATATTAAATCCTACCGTTTATCTTTTTACACGCTAATTa                                                                      >  1:3503104/1‑99 (MQ=255)
                                 ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAct                                                                    >  2:348354/1‑100 (MQ=255)
                                   cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgc                                                                  >  2:3840235/1‑100 (MQ=255)
                                    gCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                 <  1:201035/100‑1 (MQ=255)
                                       aTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCATGAAGATAATCTTTTTACACGCTAATTACTGCTGAt                                                              >  1:115537/1‑100 (MQ=255)
                                           ccTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                          >  1:2307167/1‑100 (MQ=255)
                                            cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTTCTGAtttt                                                           >  2:1207853/1‑98 (MQ=255)
                                            cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                         <  2:2321643/100‑1 (MQ=255)
                                             tCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgc                                                        <  1:391310/100‑1 (MQ=255)
                                              cGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                       <  2:2099205/100‑1 (MQ=255)
                                                  cGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCa                                                   <  1:2331095/100‑1 (MQ=255)
                                                    tctcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                 <  1:2009137/100‑1 (MQ=255)
                                                    tctcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                 <  1:1067656/100‑1 (MQ=255)
                                                    tctcAATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                 <  2:347416/100‑1 (MQ=255)
                                                      tcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTAAGCaa                                               <  1:3586599/100‑1 (MQ=255)
                                                         atatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATTTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATa                                             <  1:3106616/99‑1 (MQ=255)
                                                          tatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa                                           >  2:149622/1‑100 (MQ=255)
                                                             tGACGAATCAGCATAAATAAAAAACTGCTGTATTTTATTTCCTGCCGTTTATCTTTTAACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc                                        <  1:1247773/100‑1 (MQ=255)
                                                             tGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc                                        <  1:464580/100‑1 (MQ=255)
                                                             tGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATc                                        >  2:3753042/1‑100 (MQ=255)
                                                                cGAATCAGCATAAATAAAAAACGGCAGGATATTATTTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg                                     <  1:578680/100‑1 (MQ=255)
                                                                cGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg                                     >  2:1559969/1‑100 (MQ=255)
                                                                cGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg                                     <  1:937867/100‑1 (MQ=255)
                                                                     cAGCATAAATAAAAAACTGCAGGATATTATCTCATGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTGAGCAATAAAATCCAGCGCt                                 >  2:437766/1‑99 (MQ=255)
                                                                     cAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtt                                <  1:3350183/100‑1 (MQ=255)
                                                                         taaaaagataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                            >  2:3452223/10‑100 (MQ=25)
                                                                         ataaataaAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                            <  2:2939432/100‑1 (MQ=255)
                                                                          taaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgttg                           <  2:2829229/100‑1 (MQ=255)
                                                                          taaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATACAGCGCTttgttg                           >  1:3452547/1‑100 (MQ=255)
                                                                          taaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCACGTTCAGAAATAAAATCCAGCGCTttgtt                            >  1:1348356/1‑99 (MQ=255)
                                                                           aaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa                          <  1:1196268/100‑1 (MQ=255)
                                                                           aaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAACAAAATCCAGCGCTTTGTTGa                          <  2:2386965/100‑1 (MQ=255)
                                                                            aataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                         <  2:41827/100‑1 (MQ=255)
                                                                            aaagataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTTATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                         >  2:1011373/7‑100 (MQ=37)
                                                                             ataaaAAACGGCAGGATATTATCTCCTGCTGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa                        <  1:1525091/100‑1 (MQ=255)
                                                                             agaaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa                        >  1:2653019/3‑100 (MQ=255)
                                                                               gataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCACGTTCAGCAATAAAATCCAGCGCTTTGTTGATACg                      >  1:944107/4‑100 (MQ=37)
                                                                                ataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGc                     >  1:3189229/3‑100 (MQ=38)
                                                                                  aaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                   >  1:2541151/1‑100 (MQ=38)
                                                                                  aaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                   >  2:2624535/1‑100 (MQ=39)
                                                                                  aaaCAGCAGGTTATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTGCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                   <  2:2658844/100‑1 (MQ=255)
                                                                                   aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                   >  2:1987628/1‑99 (MQ=39)
                                                                                   aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                  >  1:817800/1‑100 (MQ=39)
                                                                                   aaCGGCAGGAGACCATATCCTGCAGCTTATCTTTTTACACGATAATTAATGCTGATGTTCGCGTTCAGCAATAGAATGACGCGCTTTGTTGATACACTc                   >  2:163420/1‑99 (MQ=255)
                                                                                    aCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGa                 >  1:2524684/1‑100 (MQ=255)
                                                                                    aCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGa                 >  1:3130222/1‑100 (MQ=39)
                                                                                       gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc              >  2:3910405/1‑100 (MQ=255)
                                                                                       gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc              >  2:3617862/1‑100 (MQ=255)
                                                                                       gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCACGATCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc              >  1:1039044/1‑100 (MQ=255)
                                                                                        cAGGATATTATCTCCTGCCGTTTATCTTTTTATTTTTTTTTTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAACGCTTTGTTGATACGCTCGATACt             >  2:246888/1‑100 (MQ=255)
                                                                                         aGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTg            >  2:898652/1‑100 (MQ=255)
                                                                                          ggATATTATCTCCTGCCGTTTATCTTTTTACACTCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGc           >  2:3397704/1‑100 (MQ=255)
                                                                                          ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGc           >  2:1032880/1‑100 (MQ=255)
                                                                                           aatatTATCTCCTGCCGTTTATCTTATTACACACTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTTATACGCTCGATACTGCg          >  2:230946/2‑100 (MQ=255)
                                                                                             tattatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCggg        <  2:309696/100‑1 (MQ=255)
                                                                                             tattatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCggg        <  2:309128/100‑1 (MQ=255)
                                                                                             tattatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCggg        <  1:2831353/100‑1 (MQ=255)
                                                                                             tattatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCggg        <  2:1363741/100‑1 (MQ=255)
                                                                                              attatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt       <  1:3723856/100‑1 (MQ=255)
                                                                                               ttatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTc      >  1:3645828/1‑100 (MQ=255)
                                                                                               ttatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTc      <  1:2589674/100‑1 (MQ=255)
                                                                                               ttatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTc      <  1:2589454/100‑1 (MQ=255)
                                                                                                tatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCt     >  2:680902/1‑100 (MQ=255)
                                                                                                  tctcCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTa   >  1:394684/1‑100 (MQ=255)
                                                                                                   ctcCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc  <  1:2664602/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAC  >  NC_000913/2519129‑2519327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: