Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 308,054 (C)7→6 coding (1279/2526 nt) ecpC ← ECP production outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913308,0480C.97.9% 423.2 / 1.6 95coding (1285/2526 nt)ecpCECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base . (50/43);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 108 total aligned reads displayed.

ACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGG  >  NC_000913/307949‑308143
                                                                                                   |                                                                                               
acaGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGc                                                                                                 <  2:1919964/100‑1 (MQ=255)
 caGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcc                                                                                                <  2:1186914/100‑1 (MQ=255)
 caGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcc                                                                                                <  2:2545627/100‑1 (MQ=255)
   gCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcccc                                                                                              <  1:107534/100‑1 (MQ=255)
   gCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcccc                                                                                              <  2:2185830/100‑1 (MQ=255)
   gCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcccc                                                                                              >  2:2099615/1‑100 (MQ=255)
   gCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcccc                                                                                              >  2:643532/1‑100 (MQ=255)
     gAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGcccccc                                                                                            <  1:2040865/100‑1 (MQ=255)
        cTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGccccccaag                                                                                         >  2:2879271/1‑97 (MQ=255)
         tAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGccccccaagc                                                                                        <  1:2851395/100‑5 (MQ=255)
         tAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGccccccaagc                                                                                        <  2:61126/100‑5 (MQ=255)
         tAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGccccccaagc                                                                                        <  2:61305/100‑5 (MQ=255)
          aaaGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGCGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCg                                                                                      <  1:584304/100‑1 (MQ=255)
            aGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGc                                                                                    <  1:1503735/100‑1 (MQ=255)
                tCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCAAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCg                                                                                <  2:796573/100‑1 (MQ=255)
                 cccGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGt                                                                               >  2:259615/1‑100 (MQ=255)
                 cccGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGt                                                                               <  2:324996/100‑1 (MQ=255)
                        gagTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACgg                                                                        >  1:2997222/1‑100 (MQ=255)
                         agTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACggg                                                                       <  2:2729197/100‑1 (MQ=255)
                          gTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGt                                                                      >  2:2862609/1‑100 (MQ=255)
                          gTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGt                                                                      >  1:1374477/1‑100 (MQ=255)
                              cgcTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTca                                                                  >  1:312065/1‑100 (MQ=255)
                              cgcTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTca                                                                  >  2:2947652/1‑100 (MQ=255)
                              cgcTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTca                                                                  <  2:388622/100‑1 (MQ=255)
                               gcTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTcac                                                                 >  2:2906421/1‑100 (MQ=255)
                                 tGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTcaccc                                                               <  1:3078715/100‑1 (MQ=255)
                                 tGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTcaccc                                                               <  1:2595290/100‑1 (MQ=255)
                                   aTGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTcacccac                                                             >  2:695101/1‑100 (MQ=255)
                                     gctgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCg                                                           >  1:1401009/1‑100 (MQ=255)
                                       tgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGcc                                                         >  1:60488/1‑100 (MQ=255)
                                       tgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGcc                                                         <  2:2905642/100‑1 (MQ=255)
                                       tgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGcc                                                         >  2:2878298/1‑100 (MQ=255)
                                       tgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGcc                                                         >  1:157628/1‑100 (MQ=255)
                                       tgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGcc                                                         <  1:1524574/100‑1 (MQ=255)
                                        gcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCt                                                        <  1:1037352/100‑1 (MQ=255)
                                         ccGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                       >  1:655983/1‑100 (MQ=255)
                                         ccGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                       <  2:655980/100‑1 (MQ=255)
                                          cGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGa                                                      <  2:2493968/100‑1 (MQ=255)
                                          cGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGa                                                      >  2:2368646/1‑100 (MQ=255)
                                           gATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGAt                                                     >  1:268512/1‑100 (MQ=255)
                                              gctgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTAt                                                  >  2:2457446/1‑100 (MQ=255)
                                               ctgctCNATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATc                                                 <  1:2626066/100‑1 (MQ=255)
                                               ctgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATc                                                 >  1:1998949/1‑100 (MQ=255)
                                                tgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCg                                                <  1:1474733/100‑1 (MQ=255)
                                                tgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCg                                                <  1:480689/100‑1 (MQ=255)
                                                tgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCg                                                <  2:1259396/100‑1 (MQ=255)
                                                 gctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGt                                               >  1:146306/1‑100 (MQ=255)
                                                   tCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTAt                                             <  2:2208529/100‑1 (MQ=255)
                                                    ccATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATc                                            >  2:1537880/1‑100 (MQ=255)
                                                    ccATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATc                                            >  2:1464673/1‑100 (MQ=255)
                                                      aTGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCa                                          <  2:735500/100‑1 (MQ=255)
                                                      aTGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCa                                          <  1:259616/100‑1 (MQ=255)
                                                       tGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCat                                         <  1:2204127/100‑1 (MQ=255)
                                                       tGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCat                                         >  1:67873/1‑100 (MQ=255)
                                                        gAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCata                                        <  1:2013142/100‑1 (MQ=255)
                                                        gAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCata                                        <  1:423926/100‑1 (MQ=255)
                                                          gCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATc                                      <  2:1375528/100‑1 (MQ=255)
                                                          gCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATc                                      >  2:1730935/1‑100 (MQ=255)
                                                           cTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATcc                                     >  2:1597853/1‑100 (MQ=255)
                                                            tGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATccc                                    <  1:1865016/100‑1 (MQ=255)
                                                              tCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGt                                  <  2:698291/100‑1 (MQ=255)
                                                               cACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGtt                                 <  1:2186090/100‑1 (MQ=255)
                                                               cACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGtt                                 >  2:822574/1‑100 (MQ=255)
                                                               cACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGt                                  >  2:1315853/1‑99 (MQ=255)
                                                                aCTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTg                                >  2:2458268/1‑100 (MQ=255)
                                                                 cTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGtt                                 >  1:2961375/1‑98 (MQ=255)
                                                                 cTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgc                               <  1:643534/100‑1 (MQ=255)
                                                                   ggCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccg                             <  2:144825/100‑1 (MQ=255)
                                                                   ggCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccg                             >  2:594649/1‑100 (MQ=255)
                                                                     ccAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccgcc                           >  2:1037752/1‑100 (MQ=255)
                                                                     ccAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccgcc                           >  1:2846597/1‑100 (MQ=255)
                                                                      cAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGccc                          >  2:2244525/1‑100 (MQ=255)
                                                                      cAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGccc                          >  1:105166/1‑100 (MQ=255)
                                                                       aGCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGccc                          <  1:1955801/99‑1 (MQ=255)
                                                                        gCATATTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAg                        >  1:51456/1‑100 (MQ=255)
                                                                           tatTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTa                     >  1:1066847/1‑100 (MQ=255)
                                                                           tatTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTa                     >  2:1311589/1‑100 (MQ=255)
                                                                           tatTTTGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTa                     >  1:187278/1‑100 (MQ=255)
                                                                             ttttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAg                   >  1:2280363/1‑100 (MQ=255)
                                                                             ttttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAg                   >  1:739831/1‑100 (MQ=255)
                                                                             ttttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAg                   >  1:259627/1‑100 (MQ=255)
                                                                              tttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGc                  >  2:718444/1‑100 (MQ=255)
                                                                              tttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGc                  <  2:755734/100‑1 (MQ=255)
                                                                               ttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCg                 <  1:1537896/100‑1 (MQ=255)
                                                                               ttGCAGGTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCg                 <  1:3011466/100‑1 (MQ=255)
                                                                                tGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCg                 >  1:2121898/1‑100 (MQ=255)
                                                                                    ggTTAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTc            <  2:481668/100‑1 (MQ=255)
                                                                                      ttAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCaa          <  1:1482509/100‑1 (MQ=255)
                                                                                      ttAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCaa          <  1:843139/100‑1 (MQ=255)
                                                                                      ttAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCaa          >  2:1056494/1‑100 (MQ=255)
                                                                                       tAACGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAAt         >  1:2298465/1‑100 (MQ=255)
                                                                                          cGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAg      >  2:470364/1‑100 (MQ=255)
                                                                                          cGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAg      >  2:361156/1‑100 (MQ=255)
                                                                                          cGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAg      <  2:1400997/100‑1 (MQ=255)
                                                                                          cGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAg      <  2:312063/100‑1 (MQ=255)
                                                                                          cGTTGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAg      >  1:305062/1‑100 (MQ=255)
                                                                                            ttGATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGcc    <  1:2057139/100‑1 (MQ=255)
                                                                                              gATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCgg  >  1:515617/1‑100 (MQ=255)
                                                                                              gATCGCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCgg  <  2:2426841/100‑1 (MQ=255)
                                                                                               aTCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCgg  <  1:806502/100‑1 (MQ=255)
                                                                                                   |                                                                                               
ACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGG  >  NC_000913/307949‑308143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: