Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,652,855 (G)6→7 coding (362/846 nt) sseA → 3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,652,8491.G96.6% 413.8 / 6.3 117coding (356/846 nt)sseA3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base G (53/60);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 131 total aligned reads displayed.

AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAT  >  NC_000913/2652750‑2652943
                                                                                                    |                                                                                                 
  gATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGCGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gg                                                                                                >  2:2161433/1‑100 (MQ=255)
   aTAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggg                                                                                               >  1:2882109/1‑100 (MQ=255)
     aaGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggg                                                                                            <  2:1750242/100‑1 (MQ=255)
      aGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggg                                                                                            >  1:1526396/1‑99 (MQ=255)
       gCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggg                                                                                          >  1:2584375/1‑99 (MQ=255)
        cACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggg                                                                                          >  1:1067304/1‑98 (MQ=255)
         aCCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtg                                                                                        >  2:24262/1‑97 (MQ=255)
           cTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtgga                                                                                      >  1:1087095/1‑95 (MQ=255)
           cTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtgga                                                                                      >  2:416077/1‑95 (MQ=255)
           cTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTAT‑‑gggggggtgga                                                                                      >  2:663508/1‑95 (MQ=255)
            tGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGAc                                                                                     >  2:1003802/1‑100 (MQ=255)
              aTTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACtt                                                                                   <  1:47346/100‑1 (MQ=255)
               ttGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTg                                                                                  <  2:1612342/100‑1 (MQ=255)
               ttGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTg                                                                                  <  1:958253/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGCGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGc                                                                                 >  2:702133/1‑100 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGGTGGACTTg                                                                                  <  2:2987657/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGc                                                                                 <  1:715646/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGc                                                                                 <  1:40432/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGc                                                                                 >  2:702100/1‑100 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                >  2:1151216/1‑100 (MQ=255)
                  tCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAg                                                                               <  1:2991956/100‑1 (MQ=255)
                  tCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAg                                                                               <  2:1098429/100‑1 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAgg                                                                              >  1:2428709/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             >  1:371660/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             >  1:3086474/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             >  2:1575226/1‑100 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCTACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                          <  2:1770652/100‑1 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                          <  1:1003813/100‑1 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                          >  1:1570764/1‑100 (MQ=255)
                             ggTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAgcgc                                                                    >  1:160350/1‑100 (MQ=255)
                             ggTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAgcgc                                                                    <  2:844712/100‑1 (MQ=255)
                              gTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGcgcg                                                                   >  1:675285/1‑100 (MQ=255)
                              gTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGcgcg                                                                   <  2:56758/100‑1 (MQ=255)
                              gTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGcgcg                                                                   <  2:29121/100‑1 (MQ=255)
                                aaTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgat                                                                 >  1:557189/1‑100 (MQ=255)
                                aaTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgat                                                                 <  1:435598/100‑1 (MQ=255)
                                aaTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgat                                                                 <  1:1858566/100‑1 (MQ=255)
                                aaTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgat                                                                 <  2:1763188/100‑1 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatg                                                                >  2:90744/1‑100 (MQ=255)
                                   cTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatgat                                                              >  1:794995/1‑100 (MQ=255)
                                   cTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatgat                                                              >  2:2092196/1‑100 (MQ=255)
                                   cTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatga                                                               <  1:2729833/99‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  2:55066/100‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             >  1:1655738/1‑100 (MQ=255)
                                      ttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctg                                                           <  2:2951218/100‑1 (MQ=255)
                                        ctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        <  1:1189368/100‑1 (MQ=255)
                                         tcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        <  2:1756473/100‑1 (MQ=255)
                                           tgCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGtt                                                      <  2:335426/99‑1 (MQ=255)
                                            gCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTa                                                     >  1:1387840/1‑100 (MQ=255)
                                              cccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTaga                                                   >  1:2546372/1‑100 (MQ=255)
                                                cACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaag                                                 <  1:231951/100‑1 (MQ=255)
                                                 aCGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaaga                                                <  2:2758231/100‑1 (MQ=255)
                                                    aGCATGGGGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                             <  1:2973645/100‑1 (MQ=255)
                                                      cATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                             <  1:1673761/98‑1 (MQ=255)
                                                      cATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTg                                           >  1:629211/1‑100 (MQ=255)
                                                      cATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTg                                           >  2:345900/1‑100 (MQ=255)
                                                       aTGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGc                                          <  1:1151226/100‑1 (MQ=255)
                                                       aTGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGc                                          >  1:237345/1‑100 (MQ=255)
                                                       aTGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGc                                          <  1:1902196/100‑1 (MQ=255)
                                                        tggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCa                                         >  2:1401333/1‑100 (MQ=255)
                                                          gtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGt                                       <  1:1733007/100‑1 (MQ=255)
                                                           tggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTa                                      <  1:1180296/100‑1 (MQ=255)
                                                           tggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTa                                      <  1:1195319/100‑1 (MQ=255)
                                                            ggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTag                                     >  1:1065753/1‑100 (MQ=255)
                                                             ctttctctacACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTaga                                    >  2:1922269/10‑100 (MQ=255)
                                                              aTGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                   >  1:3068035/1‑100 (MQ=255)
                                                              aTGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                   <  2:2648873/100‑1 (MQ=255)
                                                               tgcggcGCACCTGTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                   <  2:914406/99‑1 (MQ=255)
                                                                 ctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTg                                <  1:2437065/100‑1 (MQ=255)
                                                                  tgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGc                               >  1:1127805/1‑100 (MQ=255)
                                                                   gcgcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGcc                              <  2:2596151/100‑1 (MQ=255)
                                                                   gcgcACCTTCGGTGTAGAGACAGTGTCGATTCG‑‑GGGGGGCTGGACTTGCAGGCTGGGAGCGCTATGATCTGCGCTTAGACGAAGGTGGCGTagagacgc                               >  2:2485091/1‑95 (MQ=255)
                                                                    cgcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCg                             <  1:2479588/100‑1 (MQ=255)
                                                                    cgcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCg                             <  1:2260199/100‑1 (MQ=255)
                                                                     gcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCgg                            <  1:916769/100‑1 (MQ=255)
                                                                      taCCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGa                           <  1:2149040/99‑1 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                        <  2:1294895/100‑1 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                        >  1:1154240/1‑100 (MQ=255)
                                                                             ggTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGagag                    >  1:2604963/1‑100 (MQ=255)
                                                                              gtgtAGAGAAAGTGTCGATTCT‑‑GGGGAGGAGGACTTAAAGGCTGGTAGCGGGATGATCTGCTGTTAGAAGAAGGTGAAGTAGAGCTGCCTGaa                          >  2:2427763/1‑93 (MQ=255)
                                                                               tgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCGGTAGAGCTGCCGGAAGGAGAttt                  >  2:1625772/1‑100 (MQ=255)
                                                                                  agagAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTaa               >  2:1412234/1‑100 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTAGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAc              <  2:196007/100‑1 (MQ=255)
                                                                                     gaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcgc            <  1:24263/100‑1 (MQ=255)
                                                                                      aaaGTGTCGATTCTGGGGGGGTGGGCTTGCTGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcgcc           >  2:2346471/1‑100 (MQ=255)
                                                                                        aGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcgccgc         >  1:2990424/1‑100 (MQ=255)
                                                                                         gtgtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCATTAGAGCTGCCGGAAGGAGAGTTTAACGCcgcg        <  2:629209/100‑1 (MQ=255)
                                                                                          tgtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGt       >  1:307370/1‑100 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      <  1:2898478/100‑1 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      >  2:1168738/1‑100 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      <  1:848758/100‑1 (MQ=255)
                                                                                            tCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGttt     <  2:192468/100‑1 (MQ=255)
                                                                                            tCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGttt     <  2:1843043/100‑1 (MQ=255)
                                                                                             cGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTa    <  2:2251789/100‑1 (MQ=255)
                                                                                             cGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTa    <  1:2098634/100‑1 (MQ=255)
                                                                                              gATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTaa   >  2:1624506/1‑100 (MQ=255)
                                                                                              gATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTaa   >  1:1964613/1‑100 (MQ=255)
                                                                                              gATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTaa   <  1:1401345/100‑1 (MQ=255)
                                                                                              gATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTa    >  2:1797117/1‑99 (MQ=255)
                                                                                               aTTCTGGGGGGGTGGACGTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGGGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAt  >  2:2934387/1‑100 (MQ=255)
                                                                                                    |                                                                                                 
AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAT  >  NC_000913/2652750‑2652943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: