Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,3640CT23.1% 232.6 / 64.0 117intergenic (+17/+26)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base T (18/9);  total (63/54)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 130 total aligned reads displayed.

GTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTC  >  NC_000913/1778265‑1778463
                                                                                                   |                                                                                                   
gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGc                                                                                                     <  1:863574/100‑1 (MQ=255)
 tgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGct                                                                                                    >  1:1893966/1‑100 (MQ=255)
  gAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGctt                                                                                                   >  2:1376509/1‑100 (MQ=255)
   aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttc                                                                                                  >  2:2322747/1‑100 (MQ=255)
    aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttct                                                                                                 <  1:928954/100‑1 (MQ=255)
    aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttct                                                                                                 <  2:1354039/100‑1 (MQ=255)
    aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCATGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttct                                                                                                 <  2:682430/100‑1 (MQ=255)
      gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTa                                                                                               <  2:78343/100‑1 (MQ=255)
       tATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTaa                                                                                              >  2:2181606/1‑100 (MQ=255)
       tATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTaa                                                                                              >  2:1637443/1‑100 (MQ=255)
        aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                             >  2:1363508/1‑100 (MQ=255)
        aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                             <  1:854187/100‑1 (MQ=255)
         tCGGCGGCGGTACAGACGAAATTATGATTTACATAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACa                                                                                            <  1:2319581/100‑1 (MQ=255)
          cggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACaa                                                                                           >  1:1953655/1‑100 (MQ=255)
            gcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAAc                                                                                         <  1:1588005/100‑1 (MQ=255)
            gcggcggTACAGACGAAATTATGATTTACGTAGCAGGTAGGCAGATCCTGAAAGACTACCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACaaan                                                                                         <  2:1686633/100‑2 (MQ=255)
               gcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgc                                                                                      <  2:415897/100‑1 (MQ=255)
                  gTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgcc                                                                                   <  1:914298/100‑1 (MQ=38)
                  gTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                   <  1:2115957/100‑1 (MQ=255)
                  gTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                   >  1:2882085/1‑100 (MQ=255)
                   tACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccg                                                                                  >  1:2454037/1‑100 (MQ=255)
                     cAGACGAAATTCTGACTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                   <  2:2104910/97‑1 (MQ=255)
                     cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcc                                                                                <  2:2907550/100‑1 (MQ=38)
                     cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcc                                                                                <  2:2749842/100‑1 (MQ=38)
                     cAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCgccgcc                                                                                <  1:1335953/100‑1 (MQ=255)
                      aGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCa                                                                               >  2:977216/1‑100 (MQ=255)
                      aGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCa                                                                               >  1:1737397/1‑100 (MQ=255)
                      aGACGAAATTATGATTTACGTAGCAGGTAGGCAGATCCTGAAAGACTATCAGTACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGACGCCa                                                                               >  2:1535098/1‑100 (MQ=255)
                       gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAg                                                                              >  1:697011/1‑100 (MQ=255)
                       gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAg                                                                              >  2:1286490/1‑100 (MQ=255)
                       gACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAg                                                                              >  2:2088625/1‑100 (MQ=255)
                         cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcctgca                                                                            <  2:188536/100‑5 (MQ=25)
                         cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAt                                                                            <  1:1780533/100‑1 (MQ=255)
                         cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAt                                                                            <  2:2598991/100‑1 (MQ=255)
                          gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcctgcag                                                                           <  1:1125314/100‑6 (MQ=25)
                          gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAtt                                                                           <  1:3077245/100‑1 (MQ=255)
                           aaaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAttt                                                                          >  2:2672829/1‑100 (MQ=255)
                             aTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTAt                                                                        >  1:2543391/1‑100 (MQ=255)
                             aTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTAt                                                                        >  1:2543436/1‑100 (MQ=255)
                               tATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGCgccgcctgcagattat                                                                      <  1:2287455/100‑11 (MQ=21)
                               tATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATcc                                                                      <  1:1153663/100‑1 (MQ=255)
                                  gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTCAACAAACTGCGCCGCCAGATTTATCcaac                                                                   <  1:3013989/100‑1 (MQ=255)
                                   aTTTACGTAGCAGGGCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaaca                                                                  <  2:2098147/100‑1 (MQ=255)
                                     ttACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAg                                                                <  1:99197/100‑1 (MQ=255)
                                      tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGa                                                               <  2:27554/100‑1 (MQ=255)
                                         gTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACtt                                                            >  1:2362112/1‑100 (MQ=255)
                                          tAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                           >  1:2936757/1‑100 (MQ=255)
                                          tAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                           >  1:2809754/1‑100 (MQ=255)
                                           aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAc                                                          >  1:2583229/1‑100 (MQ=255)
                                            gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAcc                                                         >  2:2992039/1‑100 (MQ=255)
                                            gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAcc                                                         >  1:105966/1‑100 (MQ=255)
                                            gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAcc                                                         >  1:2829332/1‑100 (MQ=255)
                                                 tcgacAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTg                                                    <  2:938814/96‑1 (MQ=255)
                                                  cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGa                                                   <  1:1504212/100‑1 (MQ=255)
                                                  cGGCAGATCCAGAAAGAATATGAGAACAGATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTg                                                    <  2:409914/99‑1 (MQ=255)
                                                   ggCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                  >  1:3035182/1‑100 (MQ=255)
                                                   ggCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                  <  2:1178311/100‑1 (MQ=255)
                                                    gCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAgg                                                 <  2:188569/100‑1 (MQ=255)
                                                     cAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGa                                                >  2:637510/1‑100 (MQ=255)
                                                       gATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                              >  2:3012800/1‑100 (MQ=255)
                                                        aTCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                             <  2:2338362/100‑1 (MQ=255)
                                                        aTCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                             <  2:2813036/100‑1 (MQ=255)
                                                           cTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCa                                          <  1:1200117/100‑1 (MQ=255)
                                                            tGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAg                                         >  1:2760415/1‑100 (MQ=255)
                                                            tGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAg                                         <  1:819916/100‑1 (MQ=255)
                                                                 gACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                    >  2:152671/1‑100 (MQ=255)
                                                                   cTATCAGAACAAATAATCTGCTGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCCGTGGCGGATATTCAGATAGGAt                                  >  2:890782/1‑100 (MQ=255)
                                                                       cAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCt                              >  2:703138/1‑100 (MQ=255)
                                                                        aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGCCTTACCGGTTGAGGAAATTCAGATAGGATTTCtt                             >  1:1337923/1‑100 (MQ=255)
                                                                        aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCtt                             <  2:790081/100‑1 (MQ=255)
                                                                         gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                            >  1:1532954/1‑100 (MQ=255)
                                                                         gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                            >  1:2528767/1‑100 (MQ=255)
                                                                            cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                         >  1:1556687/1‑100 (MQ=255)
                                                                            cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                         <  1:1081129/100‑1 (MQ=255)
                                                                             aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                        >  1:2606042/1‑100 (MQ=255)
                                                                             aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                        <  1:2691732/100‑1 (MQ=255)
                                                                                taatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCa                     <  1:452577/100‑1 (MQ=255)
                                                                                gataaatctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCa                     >  2:2821722/10‑100 (MQ=21)
                                                                                gataaatctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCa                     >  1:1245903/10‑100 (MQ=21)
                                                                                  taaatctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCaaa                   >  2:474968/8‑100 (MQ=25)
                                                                                    cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTCGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                 <  1:2181629/100‑1 (MQ=255)
                                                                                    aatctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                 >  1:165146/6‑100 (MQ=25)
                                                                                      tctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAg               >  1:1507062/4‑100 (MQ=37)
                                                                                         ggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt            >  1:2638691/1‑100 (MQ=38)
                                                                                         ggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt            >  2:1129410/1‑100 (MQ=38)
                                                                                         ggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt            >  1:867791/1‑100 (MQ=38)
                                                                                         ggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt            >  1:753846/1‑100 (MQ=38)
                                                                                         ggcggcGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt            >  1:1390961/1‑100 (MQ=255)
                                                                                           cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt          >  2:938775/1‑100 (MQ=38)
                                                                                           cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt          >  1:1033596/1‑100 (MQ=38)
                                                                                           cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt          >  2:581672/1‑100 (MQ=38)
                                                                                           cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt          >  1:1632741/1‑100 (MQ=38)
                                                                                           cggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt          >  1:2109678/1‑100 (MQ=38)
                                                                                           ccgcgcAGTTTGTGAAGAAGCTGCGCCGCCAAATTTATCCAAGAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt          >  1:2084821/3‑100 (MQ=255)
                                                                                            ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTa         >  2:1104374/1‑100 (MQ=38)
                                                                                            ggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTa         <  1:70668/100‑1 (MQ=255)
                                                                                             gagcAGCTTCTTAACAAACTGCGCCGCCAGAATTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa        >  1:2076930/3‑100 (MQ=255)
                                                                                                 aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAAtag    <  1:2088649/100‑3 (MQ=255)
                                                                                                   cttcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTc  >  2:2689387/1‑100 (MQ=255)
                                                                                                   cttcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTc  <  2:1957529/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTC  >  NC_000913/1778265‑1778463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑