Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,679,4300G.21.7% 353.2 / 104.9 129intergenic (‑63/+34)yphF/yphGputative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (51/50);  new base . (13/15);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 8.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 134 total aligned reads displayed.

TAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGG  >  NC_000913/2679332‑2679529
                                                                                                  |                                                                                                    
taataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAgc                                                                                                     >  2:1444019/1‑100 (MQ=255)
 aataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAgcg                                                                                                    <  2:125245/100‑1 (MQ=255)
  ataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAgcgc                                                                                                   <  2:1197838/100‑1 (MQ=255)
   taGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGcgcg                                                                                                  <  2:2278795/100‑1 (MQ=255)
    aGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑t                                                                                                <  1:1371623/100‑1 (MQ=255)
     gATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑Tg                                                                                               <  2:2773024/100‑1 (MQ=255)
     gATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑Tg                                                                                               >  1:1519840/1‑100 (MQ=255)
       ttgcGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTACTGTAGGGTTTTTAGTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGc                                                                                              <  2:1998443/96‑1 (MQ=255)
          cGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCt                                                                                          >  1:1011916/1‑100 (MQ=255)
          cGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCt                                                                                          >  2:34426/1‑100 (MQ=255)
          cGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCt                                                                                          <  1:1253699/100‑1 (MQ=255)
           gggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTc                                                                                         <  1:2259955/100‑1 (MQ=255)
           gggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTc                                                                                         <  1:2259815/100‑1 (MQ=255)
             gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCaa                                                                                       <  1:1386368/100‑1 (MQ=255)
             gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCaa                                                                                       >  1:2564486/1‑100 (MQ=255)
              ttgttCTCGTTTTTGTAGGCATAGTGCCCCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatc                                                                                      <  2:2901968/100‑3 (MQ=255)
              ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCaaa                                                                                      <  2:592579/100‑1 (MQ=255)
              ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatc                                                                                      <  1:1822637/100‑3 (MQ=255)
              ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatc                                                                                      <  2:1188666/100‑3 (MQ=255)
              ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatc                                                                                      <  1:142535/100‑3 (MQ=255)
              ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatc                                                                                      <  1:3058067/100‑3 (MQ=255)
               tgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAat                                                                                     <  2:3056760/100‑1 (MQ=255)
               tgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatca                                                                                     <  1:641283/100‑4 (MQ=39)
               tgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatca                                                                                     <  2:2304596/100‑4 (MQ=39)
               tgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatca                                                                                     <  2:2304909/100‑4 (MQ=39)
                gttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAata                                                                                    <  2:691912/100‑1 (MQ=255)
                gttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatcac                                                                                    <  1:2777449/100‑5 (MQ=38)
                 ttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACATCGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAatat                                                                                   >  1:2537275/1‑100 (MQ=255)
                   ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAatatga                                                                                 >  1:1447937/1‑98 (MQ=255)
                     cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTTTTATCTTCGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                               >  2:2778638/1‑100 (MQ=255)
                     cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                               >  1:2876792/1‑100 (MQ=255)
                     cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                               >  2:2448011/1‑100 (MQ=255)
                     cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                               <  2:508836/100‑1 (MQ=255)
                             tAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAg                                                                       >  2:2645559/1‑100 (MQ=255)
                                gCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGt                                                                    >  2:2223235/1‑100 (MQ=255)
                                 cATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGtt                                                                   >  1:1080252/1‑100 (MQ=255)
                                  aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGttt                                                                  >  2:563403/1‑100 (MQ=255)
                                  aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGttt                                                                  <  1:203572/100‑1 (MQ=255)
                                    agagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAg                                                                >  2:166872/1‑100 (MQ=255)
                                    agagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAg                                                                <  2:324353/100‑1 (MQ=255)
                                     gagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGt                                                               <  1:2139568/100‑1 (MQ=255)
                                     gagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGt                                                               >  2:3018838/1‑100 (MQ=255)
                                       gCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCTCTCAAATATTACAGTTATTAGGGTTTAGTGa                                                             <  1:2073049/100‑1 (MQ=255)
                                          tcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcg                                                          <  2:2088162/100‑1 (MQ=255)
                                              gTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGt                                                      >  2:1242252/1‑100 (MQ=255)
                                              gTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGt                                                      <  1:34427/100‑1 (MQ=255)
                                               tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGtt                                                     >  1:1752860/1‑100 (MQ=255)
                                                aGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTa                                                    <  1:2527726/100‑1 (MQ=255)
                                                  ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaa                                                  <  1:281744/100‑1 (MQ=255)
                                                   gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                 >  2:889873/1‑100 (MQ=255)
                                                   gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                 >  1:873560/1‑100 (MQ=255)
                                                   gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                 <  1:2779010/100‑1 (MQ=255)
                                                     ttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGATCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAAt                                               <  1:1924027/100‑1 (MQ=255)
                                                       ttattaACAACGGCTTATTCTAATTATTTTGTGATGACCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaa                                             >  2:2819501/1‑100 (MQ=255)
                                                        tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                            >  2:2966001/1‑100 (MQ=255)
                                                          ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAg                                          <  1:569544/100‑1 (MQ=255)
                                                          ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAg                                          <  1:2678809/100‑1 (MQ=255)
                                                           taACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAgg                                         >  2:623151/1‑100 (MQ=255)
                                                            aacaacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGc                                        <  1:2572170/100‑1 (MQ=255)
                                                              caacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                      >  1:2778427/1‑100 (MQ=255)
                                                              caacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGATAATAGAGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                      >  2:2168846/1‑100 (MQ=255)
                                                               aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATc                                     <  1:819758/100‑1 (MQ=255)
                                                               aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATc                                     >  1:1655077/1‑100 (MQ=255)
                                                                acGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCa                                    <  1:3071437/100‑1 (MQ=255)
                                                                acGGCCTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCa                                    >  2:455898/1‑100 (MQ=255)
                                                                   gCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACgg                                 >  2:2678184/1‑100 (MQ=255)
                                                                     ttATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                               <  1:2996831/100‑1 (MQ=255)
                                                                      tATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAGAAGGCATCACggtgg                              >  1:3034179/1‑100 (MQ=255)
                                                                      tATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtgg                              <  1:2115942/100‑1 (MQ=255)
                                                                         tCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGa                           <  1:2323718/100‑1 (MQ=255)
                                                                          cTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                          >  2:1339256/1‑100 (MQ=255)
                                                                          cTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATAGTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                          >  1:911703/1‑100 (MQ=255)
                                                                           tAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaat                         >  2:1447682/1‑100 (MQ=255)
                                                                            aaTTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaata                        <  1:3046789/100‑1 (MQ=255)
                                                                               ctgtaatatttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATg                     >  2:2634246/11‑100 (MQ=37)
                                                                                   ttGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                 >  1:2546523/1‑100 (MQ=255)
                                                                                    tgtgATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCt                <  2:2150683/100‑1 (MQ=255)
                                                                                    atatttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCt                >  1:1843105/6‑100 (MQ=38)
                                                                                     tatttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCtt               >  1:1320267/5‑100 (MQ=38)
                                                                                      atttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCttt              >  2:815317/4‑100 (MQ=39)
                                                                                      atttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCttt              >  2:1827859/4‑100 (MQ=39)
                                                                                       tttgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTc             >  1:2021241/3‑100 (MQ=255)
                                                                                        ttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg            >  1:1458909/2‑100 (MQ=255)
                                                                                        ttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg            >  1:1266245/2‑100 (MQ=255)
                                                                                        ttgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg            >  2:1887458/2‑100 (MQ=255)
                                                                                        atgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg            >  1:1008298/1‑100 (MQ=255)
                                                                                        atgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg            >  2:1927215/1‑100 (MQ=255)
                                                                                         tgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg           >  1:410639/1‑100 (MQ=255)
                                                                                         tgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg           <  1:708110/100‑1 (MQ=255)
                                                                                         tgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg           >  1:2919200/1‑100 (MQ=255)
                                                                                         tgaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg           >  1:2785432/1‑100 (MQ=255)
                                                                                          gaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGc          <  1:94090/100‑1 (MQ=255)
                                                                                            gCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCa        >  2:1312353/1‑100 (MQ=255)
                                                                                             cGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCaa       >  2:241615/1‑100 (MQ=255)
                                                                                                cAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGcc    >  2:2494683/1‑100 (MQ=255)
                                                                                                cAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGcc    <  1:1614047/100‑1 (MQ=255)
                                                                                                 aGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCg   <  1:2645583/100‑1 (MQ=255)
                                                                                                 aGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCg   >  1:1605657/1‑100 (MQ=255)
                                                                                                  gcgcG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAg      <  2:1519825/96‑1 (MQ=255)
                                                                                                  gcgcG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCgg  >  1:67478/1‑100 (MQ=255)
                                                                                                  |                                                                                                    
TAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGG  >  NC_000913/2679332‑2679529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑