Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG43.3% 239.5 / inf 217intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (49/74);  new base G (93/1);  total (142/75)
Fisher's exact test for biased strand distribution p-value = 1.85e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-02

Only 100 of 218 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTC  >  NC_000913/3705885‑3706082
                                                                                                   |                                                                                                           
   ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                          <  2:2071471/100‑1 (MQ=255)
    tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                         >  2:2970780/1‑100 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                     >  1:275696/1‑100 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                     <  2:2181276/100‑1 (MQ=37)
             gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                               >  2:2419358/1‑100 (MQ=255)
             gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                               <  2:2229045/100‑1 (MQ=255)
             gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                               >  1:1464307/1‑100 (MQ=255)
                 tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                           <  1:2934439/100‑1 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                          >  1:2845674/1‑100 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                         <  2:715507/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                       <  1:1966050/100‑1 (MQ=255)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                      <  2:904642/100‑1 (MQ=255)
                          agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                  <  2:229758/100‑1 (MQ=255)
                             cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                               >  2:2132377/1‑100 (MQ=255)
                             cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                               >  1:1837613/1‑100 (MQ=255)
                             cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                               <  1:832330/100‑1 (MQ=255)
                               caGCGCTACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                             <  1:1286753/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                             <  1:3011887/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                             <  2:2857986/100‑1 (MQ=255)
                                   gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGCTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACa                                                                          >  1:433216/1‑99 (MQ=255)
                                       ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                     >  1:1375973/1‑100 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                   <  1:2558830/100‑1 (MQ=255)
                                         gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                                  >  1:658298/1‑100 (MQ=255)
                                           gTATACAGATTGTTGACAAAGTGGGCTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                                               <  1:910844/100‑1 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACTAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑tt                                                        <  2:2038711/100‑1 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑tt                                                        >  2:2933668/1‑100 (MQ=255)
                                               aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTc                                                       <  2:2212386/100‑1 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                    <  2:2369302/100‑1 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                    <  2:2478712/100‑1 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                                  <  2:1376593/100‑1 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTa                                                  <  1:1924279/100‑1 (MQ=255)
                                                   aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAg                                                 <  2:1922372/100‑1 (MQ=255)
                                                    ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAgg                                                <  2:426588/100‑1 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                              <  2:390663/100‑1 (MQ=255)
                                                       ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCt                                             <  1:2044402/100‑1 (MQ=255)
                                                       ttgACAAAGTGCGCTTTGTTGATGCCGGATGCGGCGTTAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCt                                             <  2:2772865/100‑1 (MQ=255)
                                                         gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                                           <  2:2683300/100‑1 (MQ=255)
                                                                tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                                    <  2:3061109/100‑1 (MQ=255)
                                                                tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                                    <  1:1640142/100‑1 (MQ=255)
                                                                 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                                   >  2:189679/1‑100 (MQ=255)
                                                                    gattttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                >  1:3066054/11‑100 (MQ=17)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                >  1:1369024/1‑100 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                <  1:1788351/100‑1 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                >  1:968139/1‑100 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                <  1:1879093/100‑1 (MQ=255)
                                                                       tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                             >  1:1605329/1‑100 (MQ=255)
                                                                        ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATc                            >  1:1200845/7‑100 (MQ=18)
                                                                        ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATc                            >  1:1046052/7‑100 (MQ=17)
                                                                         tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                           >  1:2281188/6‑100 (MQ=17)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                          >  2:2590805/5‑100 (MQ=18)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                          >  2:1329482/5‑100 (MQ=17)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                          >  1:502569/5‑100 (MQ=17)
                                                                           taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCT‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                         >  1:103512/4‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAgg                         >  2:3001535/4‑100 (MQ=21)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                         >  1:2416884/4‑100 (MQ=17)
                                                                           tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                         <  2:2636986/100‑1 (MQ=255)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:78956/3‑100 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc                        >  1:937427/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  1:1760422/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:2741117/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:2109148/3‑100 (MQ=17)
                                                                             tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCCTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                       >  2:1694942/1‑100 (MQ=255)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                      >  1:989317/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:474452/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:622228/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:1835910/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcga                      >  1:2884951/2‑98 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:3055622/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:595827/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTC‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:2820538/2‑100 (MQ=18)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                      >  2:1181244/1‑99 (MQ=17)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                      >  1:680887/1‑99 (MQ=17)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaa                      >  1:425815/1‑99 (MQ=25)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                      >  2:1744419/1‑99 (MQ=21)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                      >  2:84063/1‑99 (MQ=21)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                      >  1:1001982/1‑99 (MQ=21)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                      >  1:2612654/1‑99 (MQ=17)
                                                                              gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                      >  2:1773120/1‑100 (MQ=18)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTT‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaag                     >  1:773606/9‑98 (MQ=18)
                                                                               ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                     <  2:974133/100‑1 (MQ=255)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagtt                    >  2:1582028/8‑97 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                   >  2:581699/7‑100 (MQ=18)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                   >  1:2107367/7‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                    >  1:1127671/7‑97 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                   >  2:530611/7‑100 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCATACAAAACTGTGCAAATTCAATATATTGCAGGAAA‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCAgttt                   >  1:2333910/7‑100 (MQ=18)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt                  >  1:1487741/6‑100 (MQ=21)
                                                                                    taaggcgTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattttac                >  2:3040789/4‑98 (MQ=18)
                                                                                    taaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgc                >  1:1687843/4‑100 (MQ=18)
                                                                                    taaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttggc                >  2:1840739/4‑97 (MQ=18)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct              >  1:634828/1‑100 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct              >  2:896604/1‑100 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct              >  2:1440127/1‑100 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc          <  1:2227399/100‑1 (MQ=255)
                                                                                           ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc          <  2:1398535/99‑1 (MQ=255)
                                                                                           ttAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc         >  1:2250763/1‑100 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑TTTGTTTTTTTAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa        >  2:2337558/1‑100 (MQ=21)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa        <  1:2176383/100‑1 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt       <  1:35092/100‑1 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt   >  2:683446/1‑100 (MQ=255)
                                                                                                   |                                                                                                           
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTC  >  NC_000913/3705885‑3706082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: