Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,706,0480GT39.5% 292.5 / 250.6 210intergenic (‑258/+50)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (58/63);  new base T (79/0);  total (147/63)
Fisher's exact test for biased strand distribution p-value = 3.05e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 215 total aligned reads displayed.

TGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCA  >  NC_000913/3705949‑3706147
                                                                                                         |                                                                                                   
tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCgg                                                                                                     <  2:3061108/100‑1 (MQ=255)
 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGa                                                                                                    >  2:189679/1‑100 (MQ=255)
 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGa                                                                                                    <  2:2614562/100‑1 (MQ=255)
   gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAgc                                                                                                  >  1:2501464/1‑100 (MQ=255)
   gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAgc                                                                                                  <  2:526730/100‑1 (MQ=255)
    gattttgtaggCCGGATAAGGCGTTAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAgcg                                                                                                 >  1:3066054/11‑100 (MQ=17)
    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAgcg                                                                                                 <  2:211501/100‑1 (MQ=255)
      ttttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATTGTGTAAATTCAATAAATTGAT‑CG‑ATC‑‑‑CACGTAGGCCTGATAAGCGTAGCGCa                                                                                               >  2:1769391/9‑100 (MQ=21)
      ttttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAGCACGCAAACTCAATATATTGCA‑GAGATC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCa                                                                                               >  1:676191/9‑100 (MQ=17)
       tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑ACC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCAt                                                                                              >  1:818561/8‑100 (MQ=18)
        ttgtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTAC‑AAATTCTTCCAAATTCAATATATTGCA‑GA‑AAT‑‑‑CATGTAGGCCTGATAAGCGTAGCGCATc                                                                                             >  1:2376834/7‑100 (MQ=18)
        ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GA‑GTT‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATc                                                                                             >  1:2104585/7‑100 (MQ=17)
        ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑ACC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATc                                                                                             >  1:1200845/7‑100 (MQ=18)
        ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAGATCGTGCAAATTCAATATATTGCA‑TG‑AGC‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATc                                                                                             >  1:1046052/7‑100 (MQ=17)
         tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCa                                                                                            >  1:2206600/1‑100 (MQ=255)
          ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAg                                                                                           <  1:472640/100‑1 (MQ=255)
          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑ACC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                                           >  1:1897810/5‑100 (MQ=18)
          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCATGAAAATTCAATACATTGCA‑AG‑ATT‑‑‑TTCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                           >  2:1329482/5‑100 (MQ=17)
          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACTGTGCAAATTCAATATATTGCA‑GG‑AAA‑‑‑CACGTAGGCCTGATAAGCGAAGCGCATCAg                                                                                           >  2:751615/5‑100 (MQ=18)
          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAACCGCGCAAATTCAATATATTGCG‑GA‑GAA‑‑‑AATGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                          >  2:1919829/5‑100 (MQ=18)
           taggCCGGATAAGGCGTTTACGCCGCATCTGGCCTAC‑AAATTCTTCCAAATTCAATATATTGCA‑GA‑AAT‑‑‑CATGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                          >  1:2012230/4‑100 (MQ=18)
           taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAACATATTGCA‑AT‑TCT‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                          >  1:103512/4‑100 (MQ=18)
           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAGTCGTATAAATTCAATGTATTGCA‑GT‑TTA‑‑‑TTGGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                                          >  2:3001535/4‑100 (MQ=21)
           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAACATGCAAATTCAATAGATTGCA‑GAGATT‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                          >  1:1764877/4‑100 (MQ=17)
           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAACATGCAAATTCAATAGATTGCA‑GAGATT‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                          >  1:2416884/4‑100 (MQ=17)
            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAACCTGCAAATTCAATATATTGCA‑GG‑ATA‑‑‑CGTGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                                         >  2:2741117/3‑100 (MQ=18)
             tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCCTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                                                        >  2:1694942/1‑100 (MQ=255)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                        >  2:2342456/2‑100 (MQ=18)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                        >  2:1984487/2‑100 (MQ=17)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GA‑GTT‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                        >  2:622228/2‑100 (MQ=17)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAATGTGCAAATTCAATAAATTGC‑‑‑A‑ATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc                                                                                         >  2:470613/2‑100 (MQ=18)
             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑ACC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                        >  1:1835910/2‑100 (MQ=18)
             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAGTCGTATAAATTCAATGTATTGCA‑GT‑TTA‑‑‑TTGGTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                                                        >  1:1372238/2‑100 (MQ=25)
             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑ATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                        >  2:3055516/2‑100 (MQ=21)
             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACTTTGCAAATTCAATATATTGCA‑AT‑CTC‑‑‑CGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                        >  2:2820538/2‑100 (MQ=18)
              gCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                                       >  1:2808389/1‑100 (MQ=255)
              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                                       >  2:1452139/1‑99 (MQ=17)
              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                                       >  2:2873465/1‑99 (MQ=18)
              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GA‑GTT‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                                       >  2:1181244/1‑99 (MQ=17)
              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAACAGTGCAAATTCAATATATTGCA‑AT‑CTT‑‑‑CTGGTAGGCCTGATAAGCATCGCGCATCAGGCaa                                                                                       >  1:96037/1‑99 (MQ=18)
              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑ATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                                                       >  2:84063/1‑99 (MQ=21)
              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑ATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                                                       >  1:1001982/1‑99 (MQ=21)
              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACTGTGCAAATTCAATATATTGCA‑GG‑AAA‑‑‑CACGTAGGCCTGATAAGCGAAGCGCATCAGGCag                                                                                       >  1:1479721/1‑99 (MQ=18)
              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAACCTGCAAATTCAATATATTGCA‑GG‑ATA‑‑‑CGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                                                       >  2:8733/1‑99 (MQ=18)
               ccggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAACAGTGCAAATTCAATATATTGCA‑AT‑CTT‑‑‑CTGGTAGGCCTGATAAGCATCGCGCATCAGGCaag                                                                                      >  1:773606/9‑98 (MQ=18)
               ccggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑ATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                                                                                      >  1:1614901/9‑98 (MQ=21)
               ccggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑ATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                                                                                      >  1:41349/9‑98 (MQ=21)
                cggataaggcgTTCACGCCTTATCCGACCCAC‑AAAGTCTTGCAAAATCAATAAATTGCAGGA‑ACG‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaact                                                                                     >  2:1047157/8‑97 (MQ=25)
                 ggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAAGCGTGCAAATTCAATACATTGCATGG‑GCC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                                                    >  2:581700/7‑100 (MQ=18)
                 ggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GA‑GTT‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                                                    >  1:2612360/7‑100 (MQ=17)
                 ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑ATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGCaattc                                                                                    >  2:540422/7‑96 (MQ=21)
                  gATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAttttt                                                                                   >  1:588275/1‑100 (MQ=255)
                   ataaggcgTTAACGCCGCATCCGGCATAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAttttt                                                                                   >  1:2969173/5‑99 (MQ=18)
                    taaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑ACC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgc                                                                                 >  1:1687843/4‑100 (MQ=18)
                    taaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑ACC‑‑‑AT‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgc                                                                                 >  1:1708063/4‑100 (MQ=18)
                    taaggcgTTCACGCCGCATCCGGCCTAC‑AAAAGCATGAAAATTCAATATATTGCA‑GG‑AGC‑‑‑TGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttggc                                                                                 >  2:1840739/4‑97 (MQ=18)
                     gcggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                                                                                <  2:2668338/100‑1 (MQ=255)
                      cggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                                                               >  1:634828/1‑100 (MQ=255)
                           ttAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc                                                                          <  2:1613808/100‑1 (MQ=255)
                           ttAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                                                           <  2:1398535/99‑1 (MQ=255)
                           ttAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc                                                                          >  1:2250763/1‑100 (MQ=255)
                             aacgccgcaTCCGGCATAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt                                                                        >  1:2880541/9‑100 (MQ=21)
                                gCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg                                                                     >  1:2297876/1‑100 (MQ=255)
                                  cTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc                                                                   >  2:2502035/1‑100 (MQ=255)
                                       ccGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                                                              >  1:1944552/1‑100 (MQ=255)
                                       ccGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                                                              >  1:124412/1‑100 (MQ=255)
                                       ccGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                                                              <  1:2936274/100‑1 (MQ=255)
                                         ggCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATg                                                            >  1:28947/1‑100 (MQ=255)
                                         ggCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATg                                                            >  1:3010278/1‑100 (MQ=255)
                                           ccTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGc                                                          >  1:2108197/1‑100 (MQ=255)
                                             tAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                                                        >  1:184775/1‑100 (MQ=255)
                                             tAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                                                        >  1:1114653/1‑100 (MQ=255)
                                              aC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCttt                                                       >  2:783634/1‑100 (MQ=255)
                                              aC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCttt                                                       >  1:2624005/1‑100 (MQ=255)
                                               c‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                                                      <  1:1530980/100‑1 (MQ=255)
                                               c‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                                                      <  2:1192245/100‑1 (MQ=255)
                                                 aaaaTCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                                                      <  1:1352937/99‑1 (MQ=255)
                                                  aaaTCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTaa                                                    <  2:353682/100‑1 (MQ=255)
                                                   aaTCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                                                   <  2:9520/100‑1 (MQ=255)
                                                   aaTCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                                                   <  2:928456/100‑1 (MQ=255)
                                                    aTCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatt                                                  <  2:28946/100‑1 (MQ=255)
                                                        tGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGCCTTGTATGGCTTTTAATTATTc                                              <  2:1798010/100‑1 (MQ=255)
                                                          ctaaTTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTAAATTATTCGa                                            <  2:1874952/98‑1 (MQ=255)
                                                          cAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa                                            <  2:2108173/100‑1 (MQ=255)
                                                            aaTTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa                                          >  2:3036044/1‑100 (MQ=255)
                                                              ttCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATaga                                        <  2:275695/100‑1 (MQ=255)
                                                                   tACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGt                                   <  2:2297853/100‑1 (MQ=255)
                                                                          cA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGaa                            >  1:1594124/1‑100 (MQ=255)
                                                                          cA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGaa                            >  1:155422/1‑100 (MQ=255)
                                                                           a‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAg                           <  1:898905/100‑1 (MQ=255)
                                                                             gA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGt                          >  1:3084405/1‑100 (MQ=255)
                                                                                 tt‑‑‑ttCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGAt                       <  1:2432491/100‑1 (MQ=255)
                                                                                         gTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTg                  >  2:263843/1‑100 (MQ=255)
                                                                                          tAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGc                 >  2:428548/1‑100 (MQ=255)
                                                                                           aGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGcc                <  1:683449/100‑1 (MQ=255)
                                                                                            ggCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCt               >  1:426107/1‑100 (MQ=255)
                                                                                            ggCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCt               >  1:2158163/1‑100 (MQ=255)
                                                                                                tGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATg           <  2:184774/100‑1 (MQ=255)
                                                                                                 gATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATgg          >  1:1325001/1‑100 (MQ=255)
                                                                                                   tAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGAt        <  1:2335972/100‑1 (MQ=255)
                                                                                                         |                                                                                                   
TGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑ATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGTTTGCCTAATGGATCAACCA  >  NC_000913/3705949‑3706147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑