Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,397,557 G→T G49V (GGT→GTT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,5570GT99.1% 385.1 / NA 111G49V (GGT→GTT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (62/48);  minor base A (1/0);  total (63/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397461‑4397652
                                                                                                 |                                                                                               
gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTgg                                                                                              >  1:2753308/1‑100 (MQ=255)
gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTgg                                                                                              >  1:2205435/1‑100 (MQ=255)
 tctggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTggg                                                                                             <  1:2587956/97‑1 (MQ=255)
   aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGc                                                                                           <  2:1226045/100‑1 (MQ=255)
   aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGc                                                                                           <  1:1955171/100‑1 (MQ=255)
    ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTgggaga                                                                                          >  2:2587933/1‑97 (MQ=255)
    ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCg                                                                                          >  2:935613/1‑100 (MQ=255)
       ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaaa                                                                                       >  2:2507002/1‑100 (MQ=255)
       ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaaa                                                                                       >  2:869841/1‑100 (MQ=255)
       ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaaa                                                                                       >  2:660801/1‑100 (MQ=255)
       ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGaaa                                                                                       >  2:2903307/1‑100 (MQ=255)
        gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCCCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAAc                                                                                      <  1:2463533/100‑1 (MQ=255)
        ggcGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAAc                                                                                      <  1:2507971/98‑1 (MQ=255)
         tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACt                                                                                     >  1:983619/1‑100 (MQ=255)
         tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACt                                                                                     <  2:602005/100‑1 (MQ=255)
           gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTa                                                                                   <  1:2685739/100‑1 (MQ=255)
            aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTAt                                                                                  >  1:2640971/1‑100 (MQ=255)
            aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTAt                                                                                  >  1:572771/1‑100 (MQ=255)
              tgACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATcc                                                                                <  1:2340417/99‑1 (MQ=255)
               gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATccn                                                                               <  2:2350734/100‑2 (MQ=255)
               gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCg                                                                               <  1:14303/100‑1 (MQ=255)
                 ccTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCa                                                                             >  2:705570/1‑100 (MQ=255)
                  cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCAt                                                                            >  1:1995004/1‑100 (MQ=255)
                   tGCGTCGGTAGTCAAAGCACTAGTGGAAAACAGCCTCGATACAGGTGAGACGCGTATCGATATTGATATCGAACGCGATGGGGCGAAACTTATCCGCAtt                                                                           >  1:1410193/1‑100 (MQ=255)
                   tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCAtt                                                                           >  1:1097734/1‑100 (MQ=255)
                   tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCAtt                                                                           >  1:1542751/1‑100 (MQ=255)
                      gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGt                                                                        >  2:2860251/1‑100 (MQ=255)
                       tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       >  2:1228460/1‑100 (MQ=255)
                       tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       <  1:42343/100‑1 (MQ=255)
                             gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 >  2:2992557/1‑100 (MQ=255)
                             gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 >  1:982593/1‑100 (MQ=255)
                               cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1403108/1‑100 (MQ=255)
                               cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:218633/1‑100 (MQ=255)
                               cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:3031079/1‑100 (MQ=255)
                                   gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCg                                                           >  2:1697879/1‑100 (MQ=255)
                                   gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCg                                                           <  2:1988308/100‑1 (MQ=255)
                                   gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCg                                                           >  2:95238/1‑100 (MQ=255)
                                   gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCg                                                           <  1:2992587/100‑1 (MQ=255)
                                    aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATCTCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  2:912212/100‑1 (MQ=255)
                                     aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                         <  1:2457373/100‑1 (MQ=255)
                                      cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        <  1:95238/100‑1 (MQ=255)
                                       tAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        >  1:192126/1‑99 (MQ=255)
                                       tAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAt                                                       <  1:1745889/100‑1 (MQ=255)
                                        aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      >  2:2958385/1‑100 (MQ=255)
                                         gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     <  2:1704922/100‑1 (MQ=255)
                                         gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     <  2:2390280/100‑1 (MQ=255)
                                         gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  1:2226071/1‑100 (MQ=255)
                                           ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  1:606399/1‑100 (MQ=255)
                                            gAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaa                                                  <  2:72548/100‑1 (MQ=255)
                                            gAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaa                                                  >  2:48716/1‑100 (MQ=255)
                                             aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 >  2:1730971/1‑100 (MQ=255)
                                             aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 >  2:449090/1‑100 (MQ=255)
                                             aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 >  2:53428/1‑100 (MQ=255)
                                              aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  2:1044346/100‑1 (MQ=255)
                                              aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  1:2849600/100‑1 (MQ=255)
                                               aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                               <  1:394507/100‑1 (MQ=255)
                                               aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                               <  2:1928467/100‑1 (MQ=255)
                                                   gCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGa                                           <  2:218632/100‑1 (MQ=255)
                                                   gCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGa                                           >  2:1933878/1‑100 (MQ=255)
                                                    ccTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAg                                          <  1:1697897/100‑1 (MQ=255)
                                                        gATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgg                                      >  2:1697458/1‑100 (MQ=255)
                                                          tGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                    >  2:1562247/1‑100 (MQ=255)
                                                           ttcgccccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgc                                   >  1:1249880/10‑100 (MQ=21)
                                                             aGGTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctg                                 <  1:3088167/100‑1 (MQ=255)
                                                              ggTGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                >  1:928703/1‑100 (MQ=255)
                                                              gccccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                >  1:2752706/7‑100 (MQ=25)
                                                               ccccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgccggcgctggc                               >  2:2620089/6‑100 (MQ=25)
                                                                tGCGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                              >  1:1335366/1‑100 (MQ=255)
                                                                 ccaaCGCGTTCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                             >  1:1834647/4‑100 (MQ=37)
                                                                  cGACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                            <  1:177143/100‑1 (MQ=255)
                                                                   gACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           >  1:2415837/1‑100 (MQ=255)
                                                                   gACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  2:166794/100‑1 (MQ=255)
                                                                   gACGCGTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  2:2420378/100‑1 (MQ=255)
                                                                     cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  1:2903337/100‑1 (MQ=255)
                                                                     cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  1:2863212/100‑1 (MQ=255)
                                                                     cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  2:3056834/100‑1 (MQ=255)
                                                                     cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  2:2971804/1‑100 (MQ=255)
                                                                     cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  2:1329808/100‑1 (MQ=255)
                                                                     cgcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCAATAACAAAAAAGATGAgctggcgctggcgctggc                         <  2:1107024/100‑1 (MQ=255)
                                                                      gcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAACAAAGATGAgctggcgctggcgctggc                         >  2:2002622/1‑99 (MQ=255)
                                                                      gcgTATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                        <  2:2312436/100‑1 (MQ=255)
                                                                      cgcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  1:2242994/6‑100 (MQ=37)
                                                                      cgcgttCGATATCAATATCGATACGCGTCGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  1:1665509/6‑100 (MQ=25)
                                                                        cgttCGATATCAATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATTAGCTGGCGCTGGCGCTGGCTCg                      >  1:2461891/4‑100 (MQ=255)
                                                                         tATCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGt                     <  1:1965620/100‑1 (MQ=255)
                                                                          aTCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTc                    <  2:1729114/100‑1 (MQ=255)
                                                                          aTCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTc                    <  2:2489532/100‑1 (MQ=255)
                                                                           tCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   >  1:2178854/1‑100 (MQ=255)
                                                                           tCGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   <  2:572769/100‑1 (MQ=255)
                                                                            cGATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt                  <  1:256758/100‑1 (MQ=255)
                                                                             gATATTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 <  2:2597602/100‑1 (MQ=255)
                                                                               tatTGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               <  1:950393/100‑1 (MQ=255)
                                                                                 ttGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  1:2343755/1‑100 (MQ=255)
                                                                                  tGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            >  2:2181944/1‑100 (MQ=255)
                                                                                  tGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            >  1:805877/1‑100 (MQ=255)
                                                                                  tGATATCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  2:1994982/100‑1 (MQ=255)
                                                                                      atCGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGt         <  1:2205847/99‑1 (MQ=255)
                                                                                        cGAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa      >  1:870215/1‑100 (MQ=255)
                                                                                         gAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     <  1:449092/100‑1 (MQ=255)
                                                                                         gAACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     >  2:2531972/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397461‑4397652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: