BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,567	C→T	V73I (GTC→ATC)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,567	0	C	T	99.2%	418.2 / ‑4.7	122	V73I (GTC→ATC)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (56/65); total (57/65)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 122 total aligned reads displayed.

ATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAG  >  NC_000913/674470‑674663

aTGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGt                                                                                                <  1:1417902/100‑1 (MQ=255)

 tgttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGtt                                                                                               >  2:203570/1‑100 (MQ=255)

  gttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGttt                                                                                              <  2:2809013/100‑1 (MQ=255)

    tgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGttttt                                                                                            >  2:2469247/1‑100 (MQ=255)

    tgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGttttt                                                                                            >  2:1040838/1‑100 (MQ=255)

     gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGttttt                                                                                            >  2:2729450/1‑99 (MQ=255)

     gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTg                                                                                           >  2:2015823/1‑100 (MQ=255)

     gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTg                                                                                           >  1:2922717/1‑100 (MQ=255)

         tACGTCCACGGTGTCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCa                                                                                       <  2:1435459/100‑1 (MQ=255)

         tACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCa                                                                                       <  1:2371066/100‑1 (MQ=255)

         tACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCa                                                                                       >  1:368326/1‑100 (MQ=255)

          aCGTCCGCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAg                                                                                      <  2:1678443/100‑1 (MQ=255)

           cGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGc                                                                                     >  1:2057965/1‑100 (MQ=255)

            gTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCa                                                                                    >  2:828769/1‑100 (MQ=255)

             tCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCa                                                                                    <  1:27317/99‑1 (MQ=255)

             tCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCAt                                                                                   <  1:2654245/100‑1 (MQ=255)

             tCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCAt                                                                                   >  2:2335709/1‑100 (MQ=255)

              ccACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATa                                                                                  <  1:709975/100‑1 (MQ=255)

              ccACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATa                                                                                  >  2:570785/1‑100 (MQ=255)

              ccACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATa                                                                                  <  1:871082/100‑1 (MQ=255)

                 cGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCAGCAGGCAGACCAAAAGCGTCCCAGCCGATCGGGTGCAGGATGTTTTTGCCCAGCATACGc                                                                               >  2:2533873/1‑100 (MQ=255)

                  ggTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCt                                                                              <  1:2015845/100‑1 (MQ=255)

                   gTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTg                                                                             <  2:2437000/100‑1 (MQ=255)

                    tGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTgg                                                                            <  1:2204722/100‑1 (MQ=255)

                            cGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGc                                                                    <  1:47852/100‑1 (MQ=255)

                               tgttttTTTTCGCCGCCGCGCCTTCCGCAGGCAGACCAAACGCCTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATATGCTGGTAGCGGGCGAt                                                                 <  2:2616188/100‑1 (MQ=255)

                               tgttgtTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                 >  2:1481260/1‑100 (MQ=255)

                                gttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                <  2:1773924/100‑1 (MQ=255)

                                gttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                <  1:2277297/100‑1 (MQ=255)

                                gttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCAATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                <  2:1583836/100‑1 (MQ=255)

                                 ttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCa                                                               <  1:132826/100‑1 (MQ=255)

                                 ttgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCa                                                               <  1:3001923/100‑1 (MQ=255)

                                  tgttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCAc                                                              <  2:2565386/100‑1 (MQ=255)

                                    tttttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGt                                                            >  1:131043/1‑100 (MQ=255)

                                    tttttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGt                                                            <  1:2256222/100‑1 (MQ=255)

                                    tttttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGt                                                            >  1:2514138/1‑100 (MQ=255)

                                       ttCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAc                                                         <  1:828774/100‑1 (MQ=255)

                                        tCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                        <  1:2911919/100‑1 (MQ=255)

                                        tCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                        <  1:2819743/100‑1 (MQ=255)

                                        tCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                        <  2:2593054/100‑1 (MQ=255)

                                           ccgccgCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGa                                                      >  2:1356108/1‑99 (MQ=255)

                                           ccgccgCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGAt                                                     >  1:314024/1‑100 (MQ=255)

                                             gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                   <  2:16656/100‑1 (MQ=255)

                                             gccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                   <  1:1895146/100‑1 (MQ=255)

                                               cgcgCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgtg                                                 <  2:1660456/100‑1 (MQ=255)

                                               cgcgCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgtg                                                 >  2:713986/1‑100 (MQ=255)

                                                   ccTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGt                                             >  1:919401/1‑100 (MQ=255)

                                                   ccTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGt                                             >  1:1671708/1‑100 (MQ=255)

                                                    cTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                            >  2:1331728/1‑100 (MQ=255)

                                                    cTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                            >  1:865759/1‑100 (MQ=255)

                                                       ccGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacg                                         <  2:428795/100‑1 (MQ=255)

                                                       ccGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacg                                         <  1:2469270/100‑1 (MQ=255)

                                                        cGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                        >  1:610021/1‑100 (MQ=255)

                                                        cGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                        <  1:203571/100‑1 (MQ=255)

                                                        cGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                        >  1:354964/1‑100 (MQ=255)

                                                         gcaggcagACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgta                                       >  1:606405/1‑100 (MQ=255)

                                                            ggcagACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTacgtacgt                                    <  2:2004880/100‑1 (MQ=255)

                                                            ggcagACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTacgtacgt                                    >  2:36980/1‑100 (MQ=255)

                                                              cagACCAAACGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTgg                                  <  2:1273650/100‑1 (MQ=255)

                                                                    aaaCGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtg                            >  1:73225/1‑100 (MQ=255)

                                                                    aaaCGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtg                            <  1:1409600/100‑1 (MQ=255)

                                                                    aaaCGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtg                            >  2:1542607/1‑100 (MQ=255)

                                                                     aaCGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtgt                           <  2:567252/100‑1 (MQ=255)

                                                                      aCGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtgtg                          <  1:36980/100‑1 (MQ=255)

                                                                      aCGCGTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtgtg                          <  2:2767462/100‑1 (MQ=255)

                                                                        gcgTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTa                        <  1:2663639/100‑1 (MQ=255)

                                                                        atcctgcAGCCGATCGGCTGCAGGATGTTTTTTCTTTTCATTCGCTGCTAGCGGGCGATCACGTCACCTATGGTGTAGTTACGTACGTGTCCCATGTGTa                        >  2:3078149/7‑100 (MQ=255)

                                                                         tcctgcAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAg                       >  1:2639580/6‑100 (MQ=255)

                                                                         cgTCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAg                       <  1:2515188/100‑1 (MQ=255)

                                                                          cctgcAGCCGATCGGCTGCAGGATGTTTTTTTTTTTTTTTTGCTGGTAGCGGGCGATCACGTCACCGTTGTTGTAGTTTCGTGCGTGGCCCTTgtgt                         >  2:2452317/5‑97 (MQ=255)

                                                                          cctgcAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGt                      >  1:581203/5‑100 (MQ=255)

                                                                           tCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                     <  1:1356120/100‑1 (MQ=255)

                                                                           tCCCAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                     >  2:1386206/1‑100 (MQ=255)

                                                                           ctgcAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                     >  1:1154457/4‑100 (MQ=255)

                                                                            tgcAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                    <  2:1154447/98‑1 (MQ=255)

                                                                            cccAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                    <  2:1738759/100‑1 (MQ=255)

                                                                             ccAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGa                   <  2:1444849/100‑1 (MQ=255)

                                                                             ccAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGa                   >  2:464290/1‑100 (MQ=255)

                                                                              cAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                  >  2:844435/1‑100 (MQ=255)

                                                                              cAGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                  >  1:1253748/1‑100 (MQ=255)

                                                                               aGCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                 <  1:570787/100‑1 (MQ=255)

                                                                                gCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCa                >  1:2016028/1‑100 (MQ=255)

                                                                                gCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCa                <  2:34011/100‑1 (MQ=255)

                                                                                gCCGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCa                <  2:287798/100‑1 (MQ=255)

                                                                                  cGATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGa              >  1:2765462/1‑100 (MQ=255)

                                                                                   gATCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGaa             >  1:2838639/1‑100 (MQ=255)

                                                                                   gATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGaa             >  2:533184/1‑100 (MQ=255)

                                                                                    aTCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGCCCCTGTGTAGTGGACAAGaa             >  2:2422621/1‑99 (MQ=255)

                                                                                     tCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTGGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAgg           <  1:1353858/100‑1 (MQ=255)

                                                                                     tCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAgg           <  1:2729477/100‑1 (MQ=255)

                                                                                     tCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAgg           <  1:713989/100‑1 (MQ=255)

                                                                                     tCGGCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAGCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAg            <  1:1104803/99‑1 (MQ=255)

                                                                                       ggCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGAt         >  2:3013620/1‑100 (MQ=255)

                                                                                        gCTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATa        <  1:1593611/100‑1 (MQ=255)

                                                                                         cTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAg       >  2:582779/1‑100 (MQ=255)

                                                                                         cTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAg       <  2:73225/100‑1 (MQ=255)

                                                                                         cTGCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAg       <  2:2210930/100‑1 (MQ=255)

                                                                                           gCAGGATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAggg     >  1:1728104/1‑100 (MQ=255)

                                                                                              ggATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAg  <  1:2739755/100‑1 (MQ=255)

                                                                                              ggATGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAg  >  1:1904350/1‑100 (MQ=255)

ATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAG  >  NC_000913/674470‑674663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`